Fig. 5 | Communications Biology

Fig. 5

From: Rapid and gentle hydrogel encapsulation of living organisms enables long-term microscopy over multiple hours

Fig. 5

Encapsulation of a living pygmy squid hatchling in PEG hydrogel for light-sheet imaging. a Three-day-old squid hatchling was stained with 1 µM BODIPY C3 succinimidyl ester and embedded in a 1.2 mm thick, 4 mm diameter hydrogel disk and viewed in brightfield (a, b) and on a fluorescent dissecting microscope (c). d Raw light-sheet image slices of one squid arm indicated in panel c (box) are shown at 5 µm increments. Scale bars: 1 mm (a), 250 µm (b, c), 50 µm (d)

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