Fig. 3 | Communications Biology

Fig. 3

From: Anisogamy evolved with a reduced sex-determining region in volvocine green algae

Fig. 3

Sex induction and associated gene expression alternations in isogamous Y. unicocca and anisogamous Eudorina sp. a, b Asexual and sex-induced individuals of opposite sexes of Y. unicocca (plus/minus) (a) and Eudorina sp. (female/male) (b). Mating reactions (mixed, right panels) occurred after mixing induced cultures of the two sexes (middle panels). In Y. unicocca (a), clumping of the colonies and release of single-celled isogametes (arrowheads) were observed 1 h after mixing. In Eudorina sp. (b), sex induction treatment resulted in the formation of sperm packets and the packet dissociated into individual sperm that penetrated into a female colony (arrowheads) within 16 h after mixing. Scale bars, 20 µm. c, d Gene expression pattern of volvocine sex-limited genes in Y. unicocca (c) and Eudorina sp. (d). Semi-quantitative RT-PCR analyses were performed using the same cultures for a and b. All gels were run under the same experimental conditions44, and the cropped gel images are shown. Full-length gel images with size markers are presented in Supplementary Fig. 11

Back to article page