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Mass-based biocatalyst metrics to guide protein engineering and bioprocess development

Nature Catalysis volume 5, pages 2–4 (2022)Cite this article

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Metrics are a useful way to assess biocatalyst performance and, when compared to techno-economic targets, can help set goals for further enzyme and bioprocess research and development. Here, we outline some of the remaining challenges to ensure wider acceptance of this approach, both in industry and in academia.

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Fig. 1: Benchmarking cycle.

References

  1. Sheldon, R. A. & Woodley, J. M. Chem. Rev. 118, 801–838 (2018).

    Article  CAS  Google Scholar 

  2. Arnold, F. H. Angew. Chem. Int. Ed. 58, 14420–14426 (2019).

    Article  CAS  Google Scholar 

  3. Hauer, B. ACS Catal. 10, 8418–8427 (2020).

    Article  CAS  Google Scholar 

  4. Wu, S., Snajdrova, R., Moore, J. C., Baldenius, K. & Bornscheuer, U. T. Angew. Chem. Int. Ed. 60, 88–119 (2021).

    Article  CAS  Google Scholar 

  5. France, S. P., Hepworth, L. J., Turner, N. J. & Flitsch, S. L. ACS Catal. 7, 710–724 (2017).

    Article  CAS  Google Scholar 

  6. Schrittwieser, J. H., Velikogne, S., Hall, M. & Kroutil, W. Chem. Rev. 118, 270–348 (2017).

    Article  Google Scholar 

  7. Sperl, J. M. & Sieber, V. ACS Catal. 8, 2385–2396 (2018).

    Article  CAS  Google Scholar 

  8. Hönig, M., Sondermann, P., Turner, N. J. & Carreira, E. M. Angew. Chem. Int. Ed. 56, 8942–8973 (2017).

    Article  Google Scholar 

  9. de Souza, R. O. M. A., Miranda, L. S. M. & Bornscheuer, U. T. Eur. J. Chem. 23, 12040–12063 (2017).

    Article  Google Scholar 

  10. Ma, S. K. et al. Green Chem. 12, 81–86 (2010).

    Article  CAS  Google Scholar 

  11. Savile, C. K. et al. Science 329, 305–309 (2010).

    Article  CAS  Google Scholar 

  12. Huffman, M. A. et al. Science 366, 1255–1259 (2019).

    Article  CAS  Google Scholar 

  13. Ringborg, R. H. & Woodley, J. M. React. Chem. Eng. 1, 10–22 (2016).

    Article  CAS  Google Scholar 

  14. Sheldon, R. A. & Brady, D. Chem. Commun. 54, 6088–6104 (2018).

    Article  CAS  Google Scholar 

  15. Lima-Ramos, J., Tufvesson, P. & Woodley, J. M. Green Process Synth. 3, 195–213 (2014).

    CAS  Google Scholar 

  16. Lundemo, M. T. & Woodley, J. M. Appl. Microbiol. Biotechnol. 99, 2465–2483 (2015).

    Article  CAS  Google Scholar 

  17. Van Dien, S. Curr. Opin. Biotechnol. 24, 1061–1068 (2013).

    Article  Google Scholar 

  18. Bligaard, T. et al. ACS Catal. 6, 2590–2602 (2016).

    Article  CAS  Google Scholar 

  19. Kaiser, D., Yang, J. & Wuitschik, G. Org. Proc. Res. Dev. 22, 1222–1235 (2018).

    Article  CAS  Google Scholar 

  20. Takahashi, K. et al. ChemCatChem 11, 1146–1152 (2019).

    Article  CAS  Google Scholar 

  21. Lange, J.-P. Nat. Catal. 4, 186–192 (2021).

    Article  CAS  Google Scholar 

  22. Vennestrøm, P. N. R., Christensen, C. H., Pedersen, S., Grunwaldt, J.-D. & Woodley, J. M. ChemCatChem 2, 249–258 (2010).

    Article  Google Scholar 

  23. Bornadel, A. et al. Org. Proc. Res. Dev. 23, 1262–1268 (2019).

    Article  CAS  Google Scholar 

  24. Bommarius, A. S. Annu. Rev. Chem. Biomol. Eng. 6, 319–345 (2015).

    Article  CAS  Google Scholar 

  25. Woodley, J. M. Curr. Opion. Chem. Biol. 17, 310–316 (2013).

    Article  CAS  Google Scholar 

  26. Kurahashi, R., Tanaka, S.-I. & Takano, K. Enzyme Microb. Technol. 140, 109626 (2020).

    Article  CAS  Google Scholar 

  27. Truppo, M. D. ACS Med. Chem. Lett. 8, 476–480 (2017).

    Article  CAS  Google Scholar 

Download references

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Author notes

  1. Murray P. Meissner

    Present address: FabricNano, London, UK

Authors and Affiliations

  1. Department of Chemical and Biochemical Engineering, Technical University of Denmark, Lyngby, Denmark

    Murray P. Meissner & John M. Woodley

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  1. Murray P. Meissner
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  2. John M. Woodley
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Contributions

J.W. and M.P.M. contributed equally to conceptualizing this work. J.W. wrote the original manuscript. M.P.M. proofread and edited this work and generated the figure.

Corresponding author

Correspondence to John M. Woodley.

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The authors declare no competing interests.

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Peer review information

Nature Catalysis thanks Bruno Bühler, Dunming Zhu and the other, anonymous, reviewer(s) for their contribution to the peer review of this work.

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Meissner, M.P., Woodley, J.M. Mass-based biocatalyst metrics to guide protein engineering and bioprocess development. Nat Catal 5, 2–4 (2022). https://doi.org/10.1038/s41929-021-00728-5

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