Extended Data Fig. 5: Summary of SDS-PAGE analysis of samples in this study. | Nature Catalysis

Extended Data Fig. 5: Summary of SDS-PAGE analysis of samples in this study.

From: A polyketoacyl-CoA thiolase-dependent pathway for the synthesis of polyketide backbones

Extended Data Fig. 5

a, SDS-PAGE analysis of the soluble expression of BktB and 2-PS in JC01 (DE3) ΔatoB host strain. M: marker; 1: purified BktB; 2: purified 2-PS; 3: soluble fraction of JC01 (DE3) ΔatoB expressing pCDF-bktB, the arrow indicates the soluble BktB protein; 4: soluble fraction of JC01 (DE3) ΔatoB harbouring pCDF-2-ps, the arrow indicates the soluble 2-PS protein. b, SDS-PAGE analysis of the soluble expression of BktB WT and C90S, H350A, C380S mutants. M: marker; 1: soluble fraction of BL21 (DE3) expressing BktB WT; 2: soluble fraction of BL21 (DE3) expressing BktB C90S; 3: soluble fraction of BL21 (DE3) expressing BktB H350A; 4: soluble fraction of BL21 (DE3) expressing BktB C380S. c, SDS-PAGE analysis of the soluble expression of EcFadA and BktB mutants after segment swapping. M: marker; 1: soluble fraction of BL21 (DE3) expressing EcFadA WT; 2: soluble fraction of BL21 (DE3) expressing EcFadA_BktB; 3: soluble fraction of BL21 (DE3) expressing BktB WT; 4: soluble fraction of BL21 (DE3) expressing BktB_EcFadA.

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