Cellular transformations, such as gene expression or temporal protein activities, are controlled by complex stimuli-responsive network circuitries regulated by enzymes, metabolites or transcription factors. Inspired by nature, extensive research efforts are directed to mimic these processes by in vitro chemical systems. Here we report on the assembly of constitutional dynamic networks (CDNs), composed of nucleic acid–enzyme conjugates, that act as modules for triggered, network-driven, biocatalytic cascades and for the intercommunication of network-guided biocatalytic cascades. Two CDNs were assembled—one network includes a constituent module functionalized with glucose oxidase and horseradish peroxidase in spatially close positions, and the second CDN includes a constituent module modified at sterically intimate positions with nicotinamide adenine dinucleotide and alcohol dehydrogenase. Biocatalytic cascades proceed in the two networks and, on the triggered reconfiguration of the CDNs, controlled and switchable biocatalytic cascades in the CDNs are demonstrated. The two CDNs are coupled, and the triggered feedback-driven intercommunication of the networks is realized.
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The data that support the plots within this paper and other findings of this study are available from the corresponding authors upon reasonable request. Source data are provided with this paper.
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Our research is supported by the Israel Science Foundation.
The authors declare no competing interests.
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Time-dependent fluorescence changes generated by the DNAzyme reporter units associated with the constituents in CDNs and time-dependent absorbance changes of ABTS∙– in CDNs.
Time-dependent fluorescence changes generated by the DNAzyme reporter units associated with the constituents in CDNs and time-dependent absorbance changes induced by ADH/NAD+/MB+ cascade and ADH/NAD+/pyruvic acid cascade.
Time-dependent absorbance changes induced by two biocatalytic cascades guided by intercommunicating CDNs.
Time-dependent absorbance changes of two biocatalytic cascades guided by feedback-driven intercommunication of coupled CDNs.
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Wang, C., Yue, L. & Willner, I. Controlling biocatalytic cascades with enzyme–DNA dynamic networks. Nat Catal 3, 941–950 (2020). https://doi.org/10.1038/s41929-020-00524-7
Nature Catalysis (2020)