a Slices from glioma cell line-derived xenografts or patient tumors cultured on porous membranes were treated with drugs in six-well plates (one drug/well) or on the microfluidic device. A cell line drug screen guided drug choice. Responses were measured by fluorescence or tissue staining. b Oncoslice device assembly by bonding of laser-cut PMMA 40-well plate and microchannel layers. Image shows delivery of Cell Tracker Red, Cell Tracker Green, and Hoechst (blue) dyes to U87 xenograft slices. c Device cross-section shows wells, microchannel delivery to tissue slices, and a single syringe pump outlet. Scale bar: 1 cm.