Fig. 3 | npj Precision Oncology

Fig. 3

From: Label-free isolation of prostate circulating tumor cells using Vortex microfluidic technology

Fig. 3

Prostate CTC enumeration criteria. a Collected cells were enumerated using objective criteria based on immunofluorescence staining and cytomorphological characteristics, shown in the table. Each cell was assessed for each criteria in the order listed until the characteristics matched. Scale bars represent 10 µm. b The number of CTCs per 7.5 mL of blood was evaluated for our PC patient cohort (n = 20 samples), healthy donors of same age (n = 5), or donors younger than 30 years old (n = 5). The dotted line represents the threshold defined from the age-matched healthy donors as the mean number of CTCs + 2 SD, calculated to be 3.37 CTCs/7.5 mL. c For all patient samples, CTC and WBC numbers are represented, with 0–93.75 CTCs/7.5 mL and 25.00–316.88 WBCs/7.5 mL, respectively. d A diverse set of CTCs were collected from patients, which varied in size and displayed all combinations of CK and PSA expression. Scale bars represent 20 µm

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