Fig. 1 | npj Precision Oncology

Fig. 1

From: Label-free isolation of prostate circulating tumor cells using Vortex microfluidic technology

Fig. 1

Sample processing workflow with the microfluidic Vortex HT chip. Whole blood samples are collected from donors in EDTA-coated tubes. Blood is diluted 10-fold in PBS before processing through the Vortex HT microfluidic device. Purified CTCs are released in a small volume and collected in a 96-well plate. Cells are fixed, immunostained, and imaged before manual enumeration. Mutation analysis is performed using a targeted NGS panel. The Vortex HT chip consists of 16 parallel channels and 12 serial reservoirs in each channel (blue box). At high flow rates (8 mL/min), laminar microvortices develop in the rectangular reservoirs and trap larger cancer cells, while smaller blood cells (RBCs and WBCs) either pass through (red dotted box) or are not stably trapped during the wash step. Sample processing involves (i) priming the device with wash solution to remove air bubbles, (ii) infusing the sample and capturing target cells, (iii) switching to a wash solution at the same flow rate, and (iv) releasing captured cells by lowering the flow rate to dissipate the vortices

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