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  • Protocol Review
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Humane endpoints, defined

Animals used in biomedical research may experience pain or distress as part of the experimental protocol. By implementing humane endpoints, pain or distress can be prevented or alleviated whilst still meeting scientific aims and objectives. We invited experts from Arizona State University (Samantha Sullivan) and Loma Linda University (Obed Rutebuka), along with USDA and OLAW representatives (Louis DiVincenti and Axel Wolff), to respond to a scenario about the importance of defining humane endpoints in research protocols.

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  1. Institute for Laboratory Animal Research. Guide for the Care and Use of Laboratory Animals 8th edn. (National Academies Press, Washington DC, 2011).

  2. Brown, P. & Goldentyer, B. Lab Anim 51, 91 (2022).

    Article  Google Scholar 

  3. Office of Science and Technology Policy. U.S. Government Principles for the Utilization and Care of Vertebrate Animals Used in Testing, Research, and Training. Federal Register, May 20, 1985, Vol. 50, No. 97, [FR Doc. 85-12059].

  4. Public Health Service. PHS Policy on Humane Care and Use of Laboratory Animals (U.S. Department of Health and Human Services, National Institutes of Health, Bethesda, MD, 2015).

  5. Animal Welfare Act and Animal Welfare Regulations.

  6. Animal Welfare Regulations. 2023. 9 CFR § 2.31(d)

  7. Animal Welfare Regulations. 2023. 9 CFR § 2.31(c)

  8. Animal Welfare Regulations. 2023. 9 CFR § 2.31(e)

  9. Animal Welfare Regulations, 52 F.R. 10302 (proposed March 31, 1987) (to be codified at 9 CFR § 2.35)

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OLAW would like to acknowledge Neera Gopee (Associate Director for Animal Welfare Policy), Jane Na (Director, Division of Assurances), Brent Morse (Director, Division of Compliance Oversight) and Nicolette Petervary (Director, Division of Compliance Oversight) for their contribution to the preparation of the response.

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Correspondence to Lauren Danridge.

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The authors declare no competing interests.

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Disclaimer The views expressed in “A word from OLAW and USDA” represent the positions of OLAW/NIH and USDA, respectively, but the remainder of the contributions in this article do not represent official support or an endorsement by NIH, USDA, or any government agency.

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Danridge, L., Greer, B., Sullivan, S. et al. Humane endpoints, defined. Lab Anim 53, 123–125 (2024).

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