Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

GENETICS

A CRISPR cut for messenger RNAs

To achieve knockdown rather than knockout of particular genes, a new paper demonstrates a CRISPR/Cas13 method that can efficiently edit mRNA in zebrafish, medaka, killifish, and mouse embryos.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

Fig. 1

References

  1. 1.

    Pray, L. A. Nat Educ. 1, 100, https://www.nature.com/scitable/topicpage/discovery-of-dna-structure-and-function-watson-397/ (2008).

  2. 2.

    Fritz, A., Rozowski, M., Walker, C. & Westerfield, M. Genetics 144, 1734–1745 (1996).

    Google Scholar 

  3. 3.

    Solnica-Krezel, L., Schier, A. F. & Driever, W. Genetics. 136, 1401–1420 (1994).

    CAS  PubMed  PubMed Central  Google Scholar 

  4. 4.

    Chen, L., Stuart, L. & Ohsumi, T. K. et al. BMC Cancer. 13, 93, https://doi.org/10.1186/1471-2407-13-93 (2013).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  5. 5.

    Carroll, D. Genetics 188, 773–782, https://doi.org/10.1534/genetics.111.131433 (2011).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  6. 6.

    Christian, M., Cermak, T. & Doyle, E. L. et al. Genetics 186, 757–761, https://doi.org/10.1534/genetics.110.120717 (2010).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  7. 7.

    Urnov, F. D., Rebar, E. J., Holmes, M. C., Zhang, H. S. & Gregory, P. D. Nat Rev Genet 11, 636–646, https://doi.org/10.1038/nrg2842 (2010).

    CAS  Article  PubMed  Google Scholar 

  8. 8.

    Zhang, J.-H., Adikaram, P., Pandey, M., Genis, A. & Simonds, W. F. Bioengineered 7, 166–177, https://doi.org/10.1080/21655979.2016.1189039 (2016).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  9. 9.

    Wang, H., La Russa, M. & Qi, L. S. Annu Rev Biochem. 85, 227–264, https://doi.org/10.1146/annurev-biochem-060815-014607 (2016).

    CAS  Article  PubMed  Google Scholar 

  10. 10.

    Qiu, X.-Y., Zhu, L.-Y. & Zhu, C.-S. et al. ACS Synth Biol. 7, 807–813, https://doi.org/10.1021/acssynbio.7b00446 (2018).

    CAS  Article  PubMed  Google Scholar 

  11. 11.

    McCarty, N. S., Graham, A. E., Studená, L. & Ledesma-Amaro, R. Nat Commun. 11, 1281, https://doi.org/10.1038/s41467-020-15053-x (2020).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  12. 12.

    Abrams, E. W. & Mullins, M. C. Curr Opin Genet Dev. 19, 396–403, https://doi.org/10.1016/j.gde.2009.06.002 (2009).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  13. 13.

    Chen, G. R., Sive, H. & Bartel, D. P. Mol Cell. 68, 1095–1107.e5, https://doi.org/10.1016/j.molcel.2017.11.032 (2017).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  14. 14.

    Stainier, D. Y. R., Raz, E. & Lawson, N. D. et al. PLOS Genet. 13, e1007000, https://doi.org/10.1371/journal.pgen.1007000 (2017).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  15. 15.

    Joris, M., Schloesser, M., Baurain, D., Hanikenne, M., Muller, M. & Motte, P. Nucleic Acids Res. 45, 9547–9557, https://doi.org/10.1093/nar/gkx638 (2017).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  16. 16.

    Gentsch, G. E., Spruce, T., Monteiro, R. S., Owens, N. D. L., Martin, S. R. & Smith, J. C. Dev Cell. 44, 597–610.e10, https://doi.org/10.1016/j.devcel.2018.01.022 (2018).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  17. 17.

    Lai, J. K. H., Gagalova, K. K., Kuenne, C., El-Brolosy, M. A. & Stainier, D. Y. R. Dev Biol. 454, 21–28, https://doi.org/10.1016/j.ydbio.2019.06.008 (2019).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  18. 18.

    Pauli, A., Montague, T. G., Lennox, K. A., Behlke, M. A. & Schier, A. F. PLoS One. 10, e0139504, https://doi.org/10.1371/journal.pone.0139504 (2015).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  19. 19.

    Jing, X., Xie, B. & Chen, L. et al. Nucleic Acids Res. 46, e90, https://doi.org/10.1093/nar/gky433 (2018).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  20. 20.

    Aman, R., Mahas, A., Butt, H., Aljedaani, F., Mahfouz, M. Viruses. 10 2018). https://doi.org/10.3390/v10120732

  21. 21.

    Abudayyeh, O. O., Gootenberg, J. S. & Essletzbichler, P. et al. Nature. 550, 280–284, https://doi.org/10.1038/nature24049 (2017).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  22. 22.

    Kushawah, G., Hernandez-Huertas, L., Abugattas-Nuñez del Prado, J., et al. Dev Cell. August 2020. https://doi.org/10.1016/J.DEVCEL.2020.07.013

  23. 23.

    Liu, X., Homma, A., Sayadi, J., Yang, S., Ohashi, J. & Takumi, T. Sci Rep. 6, 19675, https://doi.org/10.1038/srep19675 (2016).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  24. 24.

    Lund, E., Sheets, M. D., Imboden, S. B. & Dahlberg, J. E. Genes Dev. 25, 1121–1131, https://doi.org/10.1101/gad.2038811 (2011).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  25. 25.

    Nelles, D. A., Fang, M. Y. & O’Connell, M. R. et al. Cell. 16, 488–496, https://doi.org/10.1016/j.cell.2016.02.054 (2016).

    CAS  Article  Google Scholar 

  26. 26.

    Zhang, C., Konermann, S. & Brideau, N. J. et al. Cell. 17, 212–223.e17, https://doi.org/10.1016/J.CELL.2018.09.001 (2018).

    Article  Google Scholar 

  27. 27.

    Fujita, T. & Fujii, H. Biochem Biophys Res Commun. 439, 132–136, https://doi.org/10.1016/j.bbrc.2013.08.013 (2013).

    CAS  Article  PubMed  Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to Karuna Sampath.

Ethics declarations

Competing interests

The authors declare no competing interests.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Leech, R., Sampath, K. A CRISPR cut for messenger RNAs. Lab Anim 49, 317–319 (2020). https://doi.org/10.1038/s41684-020-00661-3

Download citation

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing