To achieve knockdown rather than knockout of particular genes, a new paper demonstrates a CRISPR/Cas13 method that can efficiently edit mRNA in zebrafish, medaka, killifish, and mouse embryos.
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Leech, R., Sampath, K. A CRISPR cut for messenger RNAs. Lab Anim 49, 317–319 (2020). https://doi.org/10.1038/s41684-020-00661-3
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DOI: https://doi.org/10.1038/s41684-020-00661-3