Nat Protoc 13, 1539–1568 (2018)

Researchers in Germany have an eye-tracking solution for those who use zebrafish to study the links between visual input and oculomotor output. They’ve developed ZebEyeTrack and ZebEyeTrack Light, open-source software programs that can track the eye movements of up to six larval zebrafish at a time. The programs can analyze existing videos or, with a compatible camera, record and analyze new ones in real time. Optional external hardware, such as laser diodes and optical fibers for optogenetic manipulations, can be combined with the full version.

The authors assert that ZebEyeTrack is cheaper and more flexible than commercial tracking set-ups and quicker than processing eye movement manually. Hardware and software requirements as well as step-by-step instructions can be found in Nature Protocols.