The UF-5000 Atyp.C parameter is an independent risk factor for bladder cancer

Bladder carcinoma (BC) accounts for > 90% of all urothelial cancers. Pathological diagnosis through cytoscopic biopsy is the gold standard, whereas non-invasive diagnostic tools remain lacking. The “Atyp.C” parameter of the Sysmex UF-5000 urine particle analyzer represents the ratio of nucleus to cytoplasm and can be employed to detect urinary atypical cells. The present study examined the association between urinary Atyp.C values and BC risk. This two-center, retrospective case–control study identified clinical primary or newly recurrent BC (study period, 2022–2023; n = 473) cases together with controls with urinary tract infection randomly matched by age and sex (1:1). Urinary sediment differences were compared using non-parametric tests. The correlations between urinary Atyp.C levels and BC grade or infiltration were analyzed using Spearman’s rank correlation. The BC risk factor odds ratio of Atyp.C was calculated using conditional logistic regression, and potential confounder effects were adjusted using stepwise logistic regression (LR). Primary risk factors were identified by stratified analysis according to pathological histological diagnosis. The mean value of urinary Atyp.C in BC cases (1.30 ± 3.12) was 8.7 times higher than that in the controls (0.15 ± 0.68; P < 0.001). Urinary Atyp.C values were positively correlated with BC pathological grade and invasion (r = 0.360, P < 0.001; r = 0.367, P < 0.001). Urinary Atyp.C was an independent risk factor for BC and closely related with BC pathological grade and invasion. Elevated urinary Atyp.C values was an independent risk factor for BC. Our findings support the use of Atyp.C as a marker that will potentially aid in the early diagnosis and long-term surveillance of new and recurrent BC cases.

Bladder carcinoma (BC) is the most common urothelial malignancy, with high incidence and recurrence rates 1,2 .The gold standard for BC diagnosis is biopsy via cystoscopy, a painful, costly, and invasive type of endoscopy that places a heavy burden on both patients and healthcare providers 3,4 .Risk factors for BC development include cigarette smoking and exposure to cyclic chemicals, dyes, rubbers, textiles, and paints.Non-invasive evaluation using computed tomography (CT) revealed that bladder occupation had a suggestive effect on the early BC diagnosis, whereas the early suggestive effect of the clinical test method is unreported 4,5 .
Because the bladder is the exclusive reservoir organ for urinary cells, urinary sediments provide a source of exfoliated bladder tumor cells 6 .Using urinary samples to explore BC-related factors is cost-effective and non-invasive.Traditional urine cytology remains a mandatory screening method for patients at high risk of urothelial carcinoma in clinical practice 7,8 .The diagnostic accuracy depends on the sample type and adequacy, treatment method, underlying clinical conditions, tumor grade, and level of morphological knowledge of the examiner.In reality, the diagnostic accuracy can additionally be impacted by the presence of poorly preserved cells, inflammation, infection, and a small number of cells with irregular morphology.Thus, it can be difficult for cytopathologists to make a definitive diagnosis.The ambiguous diagnostic category of "atypical cells" has caused great distress to cytopathologists and clinicians 9,10 .Moreover, urine cytology is a time-consuming and wearisome examination 11 .Given such shortcomings, its efficacy in BC screening is greatly reduced 11 .Hence, exploring more simple and convenient urine screening markers for BC that are accurate and do not increase patient burden is necessary.
The Sysmex UF-5000 uses fluorescence flow cytometry and hydrodynamic focusing for urine sediment analysis.Intracellular particles and nucleic acids are stained with specific fluorescent dyes and then stimulated by a semiconductor laser to produce different scattered light and fluorescence signals 12,13 .The counting and

Statistical analysis
Statistical analyses were performed using IBM SPSS Statistics version 21.0 and the stats package, and plots were constructed with the ggplot2 package in R (version 4.2.1).Two-sided P-values < 0.05 were considered as statistically significant.The Mann-Whitney U test was used to analyze urinary Atyp.C and non-squamous epithelial cell (N.SEC), squamous epithelial cell (SEC), and white blood cell (WBC) count differences between the cases and controls.The significance of the difference in the distribution of Atyp.C and N.SEC, SEC, and WBC counts among pathological types was evaluated with the Kruskal-Wallis test.The correlation between urinary Atyp.C values and pathological grades and invasion was assessed using Spearman's rank correlation.The OR for urinary Atyp.C as a risk factor for BC was calculated using conditional logistic regression analysis, and the effects of potential confounders such as SEC, N.SEC, WBC, occult blood (BLD), leukocyte esterase (LEU), protein (PRO), and urinary color (COL) and turbidity (CLA) were adjusted by stepwise logistic regression.

Ethical approval
This study has been approved by the Research Ethics Committee of the University of Medical Sciences, the Second Affiliated Hospital of Medical School of Xi'an Jiaotong University (Approval No.: 2023469).

Distribution and difference analysis of urinary sediments in different pathologic types of BC
The distribution of urinary Atyp.C, N.SEC, SEC, and WBC were analyzed to account for the correlations between urinary Atyp.C and different pathological BC types.The median urinary Atyp.C gradually reduced, as the pathologic grade went from high to low or invasive to non-invasive,while N.SEC, SEC, and WBC did not show these changes (Table 2).The Kruskal-Wallis test revealed significant differences in urinary Atyp.C, N.SEC and SEC counts across the seven pathological types of BC (P < 0.05), except WBC (Fig. 3D).Furthermore, post-hoc testing (Dunnett's method) revealed that urinary Atyp.C in HMIBC was the highest and was statistically different compared with LNMIBC, other pathological types, and controls (P < 0.05).Urinary Atyp.C in the HNMIBC and PUCII group was respectively higher than that controls (P < 0.05).Urinary Atyp.C did not differ between HNMIBC, LMIBC, LNMIBC, PUNLMP, PUCII, and other pathological types (P > 0.05) (Fig. 3A).There was almost no difference in N.SEC and SEC among the different pathological types of BC, and only a slight difference was observed compared to the controls (P < 0.05) (Fig. 3B,C).Thus, the changes in Atyp.C levels were associated with the high grade and infiltration of BC, but N.SEC, SEC, and WBC did not show a corresponding relationship with BC.

Correlation between urinary Atyp.C and BC pathological grade and infiltration
The correlation coefficient (r) between urinary Atyp.C levels and pathological grade was 0.360 for controls (P < 0.001) and that between urinary Atyp.C values and infiltration was 0.367 for BC (P < 0.001).A moderate positive correlation was found between urinary Atyp.C levels and BC pathologic grades and infiltration (Fig. 4).

Urinary Atyp.C is an independent risk factor for BC
Potential risk factors for BC Random allocation was performed in a 1:1 ratio, with 473 case-control patients matched for age and sex (Table 1).Only factors with P-values of < 0.05 in univariable analysis were subsequently estimated with ORs and 95% confidence intervals (CIs) using conditional logistic regression multivariate analysis.The OR was 3.358 (95% CI, 2.33-4.841)for urinary Atyp.C; N.SEC, SEC, BLD (3+), LEU (3+), PRO, COL, and CLA were all risk factors for BC (Table 3).The Atyp.C OR was 3.488 (95% CI, 2.280-5.337),when urinary N.SEC, SEC, BLD, PRO, LEU, COL, and CLA were included in the covariates for step-by-step regression adjustment, and it was stable compared with the baseline model.The ORs for BLD (3+), LEU (3+), and COL were statistically significant and significantly changed compared with the baseline model.The ORs for urinary N.SEC, SEC, PRO, and CLA levels were not statistically significant and were excluded from the new model (Table 3).Urinary Atyp.C, BLD (3+), LEU (3+), and COL levels were identified as risk factors for BC.

Stability of potential risk factors in different BC types
The stability of the risk factors in different BC pathological types was further assessed by dividing the 237 cases with complete pathological information into five categories: high-grade BC, low-grade BC, other BCs (including PUNLMP, PUCII, and other pathological types), invasive BC, and non-invasive BC.Stratified analysis of pathological classification using conditional logistic analysis showed that only urinary Atyp.C levels significantly www.nature.com/scientificreports/correlated with BC in each type; higher urinary Atyp.C values correlated with higher ORs and a higher degree of pathological grade and invasion (Fig. 5A).Stratified analysis revealed that the correlation between BLD (3+), LEU (3+), and COL was greatly reduced with the occurrence of each BC pathological type (Figs.5B-D).In summary, elevated urinary Atyp.C levels were an independent risk factor for BC.

Discussion
The greatest obstacles to BC treatment are the high tumor recurrence rates and lack of non-invasive, inexpensive, and sensitive specific biological markers for tumor monitoring 17,18 .Over the past decades, the limitations of cystoscopy, including invasiveness and high cost, have led to the development of several urinary-based diagnostic methods for BC surveillance, including urinary cytology 10,19 , fluorescence in situ hybridization 20,21 , urinary protein detection (BTA-STAT) 22,23 , NMP22 [24][25][26] , and others 27 .However, all these biomarkers have limitations and must be combined with others for successful malignancy monitoring, which adds to the financial burden on patients 27 .This study expected to screen more risk factors from routine inspection that could detect early BC stages to help quicken diagnosis and treatment, avoid missed diagnoses, and reduce the burden on patients.Urinary Atyp.C is a parameter used in UF-5000 that is measured using a non-invasive, fast, and inexpensive assay.As atypical/malignant cells tend to exhibit excessive chromatin proliferation, the dye assessed by UF-5000     urinary samples can cross the cell membrane and bind to nucleic acids, leading to differences in the fluorescent staining of uroepithelial cells based on the nucleic acid content.Atypical cells with abnormally high nucleic acid levels can be detected using the lateral fluorescent signal waveform region.This technique could be a key tool for the early diagnosis and monitoring of BC.Urinary Atyp.C has been employed to diagnose urothelial carcinoma, with a 59.0% sensitivity, 82.1% specificity, 75.0%positive predictive value, 68.8% negative predictive value, and 71.1% coincidence rate with urinary cell exudation 16 .These results show that urinary Atyp.C analysis can indicate the number of typical/malignant urothelial cells, which is consistent with urinary Atyp.C being an independent risk factor for BC.Despite these findings, their sample size was small, and the specific cutoff range was not determined.The current study increased the sample size and found that urinary mean Atyp.C levels in BC (1.30 ± 3.12) were 8.7 times higher than those in controls without BC (0.15 ± 0.68).Levels were additionally higher in high-grade and/or invasive BC, which is consistent with the conclusion of Ren et al. that urinary Atyp.C intensity may be a good indicator of histological diagnosis of high-grade urothelial carcinomas 16 .The present study found that urinary Atyp.C values positively correlated with tumors' pathologic types (r = 0.360) and infiltration (r = 0.367).Urinary Atyp.C, N.SEC, SEC, BLD (3+), LEU (3+), PRO, COL, and CLA were identified as risk factors for BC with univariate or age-and sex-adjusted ORs estimation using logistic regression analysis.The OR for urinary Atyp.C was 3.358 (95% CI 2.33-4.814).Only urinary Atyp.C, BLD (3+), LEU (3+), and COL were statistically significant after multiple stepwise regression analysis on the above potential risk factors, with the OR for urinary Atyp.C being 3.488 (95% CI 2.28-5.337).The change in the OR for urinary Atyp.C remained stable after multi-factor adjustment.The adjusted risk factors were verified after stratification of the pathological diagnosis, and only the OR for urinary Atyp.C was always greater than 1, which was statistically significant.Higher ORs for urinary Atyp.C levels correlated with a greater risk of increasing grade or invasion.In conclusion, the increase in urinary Atyp.C values was an independent risk factor for BC in urinary sediment analysis, and the levels of urinary Atyp.C positively correlated with the degree of grade or invasion.
This study had some limitations that should be acknowledged.First, only Atyp.C was analyzed as a risk factor for BC, with other known risk factors for BC, such as smoking, heavy use of antibiotics, and common history, omitted.Moreover, urinary Atyp.C values were easily affected by high squamous cell counts and pyuria, and its repeatability was unstable owing to different urinary sample qualities and clinical treatments and interventions in the same patient 15,16 .Therefore, using urinary Atyp.C levels alone as a diagnostic index is difficult.However, the construction of a diagnostic model combining urinary Atyp.C values with other risk factors may reduce the uncertainty from its influencing factors and improve diagnostic stability.Hence, we will focus on this in future research.
In summary, this study demonstrated that urinary Atyp.C was significantly elevated in patients with BC and was associated with tumor grade and invasion.Elevated Atyp.C levels may be an independent risk factor in patients with BC.Our results provide a potentially useful diagnostic indicator for the early diagnosis of patients with BC and suggest that urinary Atyp.C may be an important diagnostic tool that deserves further investigation.

Figure 2 .
Figure 2. Differences among the distributions of Atyp.C, N.SEC, SEC and WBC in urinary sediment analysis results of cases and controls.N.SEC, non-squamous epithelial cell; SEC, squamous epithelial cell; WBC, white blood cell.

Figure 4 .
Figure 4. Correlation analysis between urinary Atyp.C and BC pathological grade or infiltration.BC, bladder carcinoma.