Association of systemic inflammatory indices with anthropometric measures, metabolic factors, and liver function in non-alcoholic fatty liver disease

The present cross-sectional study aimed to explore the relationship between systemic inflammatory indices (SIIs) and anthropometric measures, metabolic, and liver function biomarkers in patients with non-alcoholic fatty liver disease (NAFLD). This study was carried out on 238 NAFLD patients with overweight or obesity, aged 18–55 years. Anthropometric measurements were done and body mass index (BMI), waist-to-hip ratio (WHR), and waist-to-height ratio (WHtR) were estimated. Metabolic factors including serum glucose, lipid profile, liver function biomarkers, and complete blood cell count were assessed after a 24-h fasting state. SIIs including the ratios of neutrophil to lymphocyte (NLR), monocytes to lymphocyte (MLR), platelet to lymphocyte (PLR), and monocytes to high-density lipoprotein cholesterol (MHR) were calculated. Results indicate that apart from PLR, all of the SIIs significantly changed by increasing steatosis severity (all p < 0.05). Moreover, changes in NLR showed a significant association with anthropometric indices including waist circumference (p = 0.032), BMI (p = 0.047), and WHtR (p = 0.002), as well as levels of fasting blood sugar (p = 0.045), triglycerides, (p = 0.025) and low-density lipoprotein cholesterol (p = 0.006). The findings also indicate the relations between lipid profile and all studied SIIs, notably MHR and MLR. All of the SIIs exhibited associations with some liver function indices as well. MHR was positively correlated with the metabolic risk factors of NAFLD while, oppositely, PLR was considered as a preventive marker of NAFLD.


Study design
The present cross-sectional study was done on 238 NAFLD patients with overweight or obesity (body mass index (BMI) ≥ 25 kg/m 2 ) (both genders and ages ranged from 18 to 55 years).The diagnosis of NAFLD was done by ultrasonography based on Hamaguchi et al. 13 definition.The patients who were pregnant, lactating, menopause, athletes, bariatric surgery, suffering from other chronic diseases and malignancies, receiving antihypertensive, anti-hyperlipidemic, and anti-diabetic medications as well as oral contraceptives, nutritional supplements three months before the study were excluded.The study protocol was approved by the Ethics Committee of Tabriz University of Medical Science (IR.TBZMED.REC.1401.689).Moreover, informed consent was fulfilled for each subject after considering the study criteria and full explanation regarding study objectives and protocol.

Sample size
The sample size was estimated as 238 according to the prevalence rate of NAFLD in Iran, reported by Moghaddasi far et al. 14 and by considering a 95% confidence level and 6% error using G-power software (Heinrich-Heine-Universität Düsseldorf, Düsseldorf, Germany).The calculation resulted in a total of 231 sample size.Considering the possiblity of nonrespodents during the data collection process, the number of participants was increased by 15%, leading to a total of 264 participants.

Data collection
Personal details including demographic and socioeconomic status as well as behavioral details e.g.smoking, alcohol drinking, medical history, and taking supplements were obtained through a face-to-face interview by a trained nutritionist.Weight and height were measured in fasting state using a calibrated stadiometer (Seca, Hamburg, Germany) with light cloths and no shoes to the nearest 100 g and 5 mm, respectively.Waist circumference (WC) was measured at the midpoint between the lower border of the rib cage and iliac crest with 0.1 cm precision whereas hip circumference (HC) was assessed around the widest part of the buttocks to the nearest

Laboratory assay
After 12-14 h of overnight fasting, 10 ml blood samples were collected and divided into two tubes; 5 ml whole blood in EDTA-containing vacuum blood collection tubes (Vacutainer K2E) and 5 ml vacuumed gel separator tubes to be centrifuged for sera and then stored at -80 •C until the end of the study.Automated and daily-calibrated Coulter CBCH1 counter was used for assessing total white blood cell count, neutrophil, lymphocyte, monocyte count per mm3 as well as platelets count in whole blood and then, the ratios NLR, MLR, PLR and MHR were calculated as SIIs.In addition, the sera were used to determine metabolic parameters including fasting blood sugar (FBS), triglycerides (TG), total cholesterol (TC), and HDL-C using commercial kits (Pars-Azmoon Co., Tehran, Iran), and an auto-analyzer (Hitachi 902, Tokyo, Japan) as well as alanine aminotransferase (ALT) and aspartate aminotransferase (AST) based on the International Federation of Clinical Chemistry procedure to calculate AST to ALT ratio (AST/ALT) 15 .Electrochemiluminescence immunoassay was applied to determine serum ferritin levels.Then, low-density lipoprotein cholesterol (LDL-C) was estimated using Friedewald formula as follows 16 :

Statistical analysis
Data was analyzed using Statistical Package for Social Analysis (SPSS Inc., ver. 20, Chicago, IL, USA).The Kolmogorov-Smirnov test was used to check the normality distribution of continuous variables whereas Chi-square test was used for the relationship between categorical variables.Differences in symmetric and asymmetric continuous variables were done using two-independent sample t-and Mann-Whitney U-tests between the two groups, respectively.For comparing the mean of the studied variables in more than two groups, analysis of variance was applied.The association between continuous variables was checked using Pearson and Spearman correlation coefficient for symmetric and asymmetric data, respectively.p < 0.05 was considered as statistically significant.

Ethics approval and consent to participate
All procedures performed in this study were in accordance with the ethical standards of the Ethics Committee of Tabriz University of Medical Science (IR.TBZMED.REC.1401.689)and all eligible patients signed an informed written consent form.

Results
Table 1 shows participants' characteristics.More than half of the participants were female, married, and with university degrees.Overweight and mild steatosis were found in 71% and 58.8% of the studied patients, respectively.Most of the patients showed central obesity in terms of WC and WHtR, while around one-third of them had high WHR.
Between-gender comparisons in the studied parameters are shown in Table 2.There were significant differences in all anthropometric measures and indices between the genders, i.e. mean WC and WHR were significantly greater in males than females while mean BMI and WHtR were higher in women than men (p < 0.05).Among metabolic factors, significant between-gender differences in serum levels of HDL-C, AST, ALT, AST/ALT, and ferritin were found (p < 0.001).Mean platelets and monocytes were statistically different between genders (p < 0.001 and p = 0.012, respectively).Although MHR in males was significantly greater than females (p < 0.001), MLR and PLR were higher in women than men (p = 0.047 and p = 0.005, respectively).
Table 3 demonstrates differences in anthropometric measures, metabolic factors, liver function, and SIIs by the severity of liver steatosis.By increasing the severity of liver steatosis, all of the anthropometric indices (except for HC) and metabolic parameters ( apart from TC and HDL-C) increased significantly (p < 0.05).Furthermore, liver function indices changed parallel with changes in NAFLD severity in terms of significant increases in ALT, AST, and ferritin as well as decreases in AST/ALT ratio.WBC (p < 0.001), monocytes (p = 0.035), and neutrophils (p < 0.001) also enhanced by the hepatosteatosis severity.Indeed, apart from PLR, all of the SIIs were significantly greater in grade II than in grade I liver steatosis (all p < 0.05) (Table 3).
The correlations between SIIs and anthropometric indices, metabolic and liver function biomarkers are presented in Table 6.Only NLR was significantly correlated with BMI (r = 0.160, p = 0.013) while MLR and PLR were significantly correlated with liver function biomarkers, particularly in females and moderate steatosis.In addition, there were significant correlations between serum ferritin and all SIIs, apart from PLR. Withingender associations between SIIs and the studied factors were tested (Data not presented in Tables), i.e. among females, LDL-C was significantly correlated with MHR (r = 0.266, p = 0.002), MLR (r = 0.191, p = 0.003), and NLR (r = 0.176, p = 0.045) whereas PLR was found to be correlated with serum ALT (r = -0.245,p = 0.004), AST/ALT ratio (r = 0.229, p = 0.008) and ferritin (r = 0.194, p = 0.025).

Discussion
The present study investigated the association of a number of SIIs including MHR, MLR, NLR, and PLR with metabolic and anthropometric factors and also liver function in patients with NAFLD.The detection of chronic inflammation is considered a crucial goal for clinical specialists.Inflammatory biomarkers such as C-reactive protein and pro-inflammatory cytokines (e.g.interleukin 6 and tumor necrosis factor-α have been commonly studied to identify inflammatory states 17 .Furthermore, hepatosteatosis screening and monitoring are very important for the prevention of severe complications such as liver fibrosis and thereby finding reliable and simple markers of NAFLD in the blood seems to be necessary 3 .There is a growing recognition toward the critical role of systemic inflammation (intra-and extra-hepatic) assessment by relatively novel inflammatory indices such as NLR or MHR in NAFLD using a rapid, inexpensive, and routine blood test 2 .
Results of the present study demonstrated that apart from PLR, all of the SIIs significantly changed by increasing steatosis severity.Moreover, changes in NLR showed a significant association with anthropometric indices including WC, BMI, and WHtR, as well as levels of FBS, TG, and LDL-C.The findings also indicate the relations between lipid profile and all studied SIIs, notably MHR and MLR.All of the SIIs exhibited associations with some liver function indices as well.MHR was positively correlated with the metabolic risk factors of NAFLD while, oppositely, PLR was considered as a preventive marker of NAFLD.Our results also showed that anthropometric measures, liver function biomarkers, MHR, and MLR were significantly different between the genders.There is cumulative evidence indicating that inflammatory conditions more frequently occur in men whereas chronic inflammatory diseases with lower mortality rates mostly affect women [18][19][20] .
We documented significant increases in SIIs (in terms of MHR, MLR, and NLR) parallel to the changes of all anthropometric and liver function indices as well as lipidemia, by an increasing trend in hepato-steatosis severity.In this context, the association between inflammation, SIIs, and dyslipidemia has been well established in many conditions such as rheumatoid arthritis, neurological disease, depression, and mood disorders [20][21][22][23] .For example, Gong et al. 24 in their study on patients with ischemic stroke showed that SIIs including NLR, PLR, and lymphocyte-monocyte ratio (LMR) were greater in those with neurological problems than controls.It is suggested that high PLR values could be used for the prediction of thrombosis and inflammatory disturbances www.nature.com/scientificreports/as well as neurological outcomes in patients with acute ischemic stroke 24 .Additionally, the effectiveness of PLR in the prediction of all-cause and cardiovascular mortality in the maintenance of patients undergoing hemodialysis is also documented 25 .Moreover, SIIs have been implicated in the assessment of endocrine, metabolic, and CVDs, as well as cancers 2,6,26 .Despite the relatively weak association found between anthropometric indices (BMI, WC, and WHtR) and serum FBS levels with changes in NLR values in this study, other SIIs failed to present any relation to those factors.The findings also indicate the relations between lipid profile and all studied SIIs, notably MHR and MLR.Although these associations are not well established by previous studies on obesity-related conditions such as Mets and NAFLD yet, patients with Mets have shown significantly higher MHR, NLR, and PLR whereas lower LMR compared with healthy subjects 10,[27][28][29][30] .However, it should be noted that gender, BMI, and fasting hyperglycemia could influence SIIs and thereby, lead to variations in findings 26 .Our data shed light on the use of NLR, MHR, and MLR for the assessment of metabolic outcomes of NAFLD; namely by the hypothesis on the ability of MHR and MLR in the assessment of lipidemia as well as the application of NLR in obesity and glycemia assessment.Based on current evidence, MHR has been suggested for showing the complex interplay between pro-inflammatory/pro-atherogenic factors as well as lipid profile 26 .Therefore, it appears that high MHR might be a useful marker for the evaluation of lipidemia in NAFLD and other metabolic diseases.
Regarding the possible application of NLR for the assessment of glycemia, in line with our results, several studies have reported the relationship between higher NLR and poor glycemic control, macro-and microvascular diabetic complications in T2DM patients 31,32 .It appears that NLR could be used as a predictive marker of glycemic status in metabolic disorders.
As NAFLD is the hepatic manifestation of metabolic dysregulations, it involves a complex interplay among factors including excessive body fat deposition, impaired glucose and lipid homeostasis, oxidative stress, and chronic inflammation 33 .The progression of pro-inflammatory signaling pathways leads to impairments in liver function characterized by hepatic histologic findings 34 .To date, limited studies have investigated the relationship www.nature.com/scientificreports/ between SIIs and NAFLD 2,12,35,36 .In the present study, all of the SIIs showed correlations with a number of liver function biomarkers.These findings suggest increased MHR and PLR values as the progressive and preventive markers of NAFLD, respectively.A recent study reported the relationship between NLR and PLR with NAFLD, after adjusting for potential confounders 12 .Similar to our findings, Zhao et al. 2 reported that MHR was significantly higher in patients with NAFLD than in healthy controls and suggested that MHR is strongly associated with the risk of NAFLD compared with NLR, PLR, and LMR.Indeed, Huang et al. 37 reported that MHR was significantly correlated with the high risk of NAFLD, even after adjusting for age, sex, BMI, WC, ALT, TG, TC, FBS, and blood pressure.MHR is calculated based on monocytes, which are considered an important component of the immune system and a vital mediator of both chronic and acute inflammation, and thereby, induces immune-inflammatory responses 38 .In addition, HDL-C by producing an anti-inflammatory response to macrophages and mediating cholesterol consumption could be necessary for the prevention of inflammatory diseases 39 .Therefore, increased MHR is associated with diabetic nephropathy, predicting Mets and NAFLD 10,40 .
Regarding PLR, several studies similarly suggested that PLR may be more useful as a marker in determining the increased thrombotic state and inflammatory response in morbid obesity, obstructive sleep apnea-hypopnea syndrome, overall survival in patients with NAFLD-related hepatocellular carcinoma 5,36,41,42 .Taken together, it seems that PLR has relatively higher diagnostic accuracy for liver fibrosis 35 .It appears that the combination of NLR and MLR (as the markers of anthropometric and lipid-related changes) with PLR and MHR (as the indicators of liver function and metabolic regulation) could present a comprehensive perspective of NAFLD.
Accordingly, these findings could be explained by the potent role of inflammation, in terms of peripheral blood leukocytes in the progression of NAFLD 43 .Stimulated hepatocyte neutrophil infiltration is followed by macrophage recruitment and cellular damage, which activates pro-inflammatory pathways as well as oxidative stress 43 .In addition, liver stellate cells facilitate the production of hepatic reactive oxygen species and the progression of liver fibrosis through increasing neutrophils survival.Current evidence also suggests an interplay between platelets and pro-inflammatory cells, which is followed by increased chemokine and immune-inflammatory mediator secretion, which subsequently induce NAFLD 43 .Taken together, changes in the balance and the total Table 3. Participants' characteristics by the severity of steatosis.Mean ± standard deviation (SD) is presented for data.Bold values indicate statistically significant p < 0.05.WC waist circumference, HC hip circumference, BMI body mass index, WHR waist to hip ratio, WHtR waist to height ratio, FBS fasting blood sugar, TG triglycerides, TC total cholesterol, HDL-C high-density lipoprotein cholesterol, LDL-C low-density lipoprotein cholesterol, ALT alanine aminotransferase, AST aspartate aminotransferase, AST/ALT AST to ALT ratio, WBC white blood cell count, MHR monocyte to HDL-C ratio, MLR monocyte to lymphocyte ratio, NLR neutrophil to lymphocyte ratio, PLR platelets to lymphocyte ratio.*p-value for Two-independent Sample t-test.**p-value for Mann-Whitney U-test.www.nature.com/scientificreports/count of neutrophils, lymphocytes, and platelets in the blood could reflect a systemic inflammatory state and reflect the risk of NAFLD and its related outcomes 43 .Some limitations should be acknowledged when interpreting the findings of this study.First, the nature of cross-sectional cannot make a judgment regarding the causal relationship between SIIs and NAFLD.Larger sample size with various spectrum of NAFLD severity in different age groups is needed to confirm these results.Furthermore, sarcopenia, which is a progressive and systemic skeletal muscle disease, has attracted considerable attention as one of the pathological conditions involved in the development and progression of NAFLD.Sarcopenia has been reported to be closely associated with NAFLD.It causes sarcopenia-related obesity, which increases the risk of nonalcoholic steatohepatitis, liver fibrosis, and CVD [44][45][46] .However, due to the lack of data on sarcopenia status in our patients, we were unable to explore the association of SIIs with sarcopenia in the current study, which is suggested to be evaluated in future studies.The current study has several strengths too.

Table 2 .
Participants' characteristics by gender.Mean ± standard deviation (SD) is presented for data.Bold values indicate statistically significant p < 0.05.WC waist circumference, HC hip circumference, BMI Body mass index, WHR waist to hip ratio, WHtR waist to height ratio, FBS fasting blood sugar, TG triglycerides, TC total cholesterol, HDL-C high-density lipoprotein cholesterol, LDL-C low-density lipoprotein cholesterol, ALT alanine aminotransferase, AST aspartate aminotransferase, AST/ALT AST to ALT ratio, WBC white blood cell count, MHR monocyte to HDL-C ratio, MLR monocyte to lymphocyte ratio, NLR neutrophil to lymphocyte ratio, PLR platelets to lymphocytes ratio.*p-value for Two-independent Sample t-test.**p-value for Mann-Whitney U-test.

Table 4 .
Association of MHR and MLR with anthropometric indices, metabolic and liver function biomarkers.Mean ± standard deviation (SD) is presented for data.Bold values indicate statistically significant p < 0.05.MHR monocyte to HDL-C ratio, MLR monocyte to lymphocyte ratio, BMI body mass index, WC waist circumference, WHR waist to hip ratio, WHtR waist to height ratio, FBS fasting blood sugar, TG triglyceride, TC total cholesterol, HDL-C high-density lipoprotein cholesterol, LDL-C low-density lipoprotein cholesterol, ALT alanine aminotransferase, AST aspartate aminotransferase, AST/ALT AST to ALT ratio.*p-value for trend (Analysis of Variance (ANOVA)).

Table 5 .
Association of NLR and PLR with anthropometric indices, metabolic and liver function biomarkers.Mean ± standard deviation (SD) is presented for data.Bold values indicate statistically significant p < 0.05.NLR neutrophil to lymphocyte ratio, PLR platelets to lymphocyte ratio, BMI body mass index, WC waist circumference, WHR waist to hip ratio, WHtR waist to height ratio, FBS fasting blood sugar, TG triglyceride, TC total cholesterol, HDL-C high-density lipoprotein cholesterol, LDL-C low-density lipoprotein cholesterol, ALT alanine aminotransferase, AST aspartae aminotransferase, AST/ALT AST to ALT ratio.*p-value for trend (Analysis of Variance (ANOVA)).

Table 6 .
Correlations of systemic inflammatory indices with anthropometric indices, metabolic and liver function biomarkers.Bold values indicate statistically significant p < 0.05.MHR monocyte to HDL-C ratio, MLR monocyte to lymphocyte ratio, NLR neutrophil to lymphocyte ratio, PLR platelets to lymphocytes ratio, BMI body mass index, WC waist circumference, WHR waist to hip ratio, WHtR waist to height ratio, FBS fasting blood sugar, TC total cholesterol, TG triglyceride, HDL-C high-density lipoprotein cholesterol, LDL-C low-density lipoprotein cholesterol, ALT alanine aminotransferase, AST aspartae aminotransferase, AST/ALT AST to ALT ratio.*r(p), p-value for Spearman correlation coefficient.