The associations between plant-based dietary indices with depression and quality of life and insomnia among Iranian adolescent girls in 2015

Although previous research has explored the link between plant-based diets and mental health outcomes, there has been limited study on the quality levels of plant foods in this context. This study was conducted on 733 adolescent girls from cities in northeastern Iran. The validated Iranian version of the Insomnia Severity Index, SF-12v2 questionnaire and Persian version of the Beck Depression Inventory used to assess insomnia and poor quality of life (QoL) and depression, respectively. Dietary intakes assessed using a valid and reliable food frequency questionnaire. The association of scores of plant based dietary index (PDI) and poor QoL, depression and insomnia explored by binary logistic regression. The unadjusted model showed subjects in the highest quartile of healthy PDI had lower chances of insomnia than those in the lowest quartile (OR: 0.50; 95% CI 0.27–0.91, P = 0.024). The association persisted across various adjusted models. Subjects in the highest quartile of unhealthy PDI (uPDI) had higher chances of depression than those in the lowest quartile (OR: 1.83; 95% CI 1.09–3.08, P = 0.022). The significance of the association was maintained after adjusting for other confounders. A healthy plant-based dietary index is associated with a lower odds of insomnia. An unhealthy plant-based dietary index was associated to an increased chance of depression. Findings need to be confirmed by future studies.


Demographic and anthropometric assessments
Data about age, smoking status, menstruation status, medical history, supplement intake, taking psychological treatment and chronic diseases were assessed through a demographic questionnaire which was administered.Trained investigators measured anthropometric variables including weight, height and waist circumference using the standard protocols.Body mass index (BMI) is computed as weight (kg) divided by the square of height (m 2 ).Level of physical activity measured by the validated Modifiable Activity Questionnaire 23 .Physical activity was calculated based on metabolic equivalent task minutes per week.

Dietary assessment
A 147-item food frequency questionnaire (FFQ) was utilized to assess dietary intake patterns 24 .The validity and reliability of the questionnaire have been documented in prior studies 25 .Participants answered questions regarding their daily, weekly, monthly and yearly consumption, as well as the frequency of food items consumed over the past year.The reported portion sizes in the FFQ were converted to grams using household measures, and the energy and nutrient intakes were calculated using the Nutritionist IV software 26 .
We created 3 versions of a plant-based diet index using dietary data: PDI; hPDI; and uPDI, as established in prior research 27 .Eighteen food groups are categorized into three main classes: healthy, unhealthy plant foods and animal foods.Healthy food groups include fruits, vegetables, whole grains, legumes, vegetable oils, nuts, tea and coffee.Less healthy food groups include sugar-sweetened beverages, refined grains, fruit juices, potato, sweets and desserts.Animal food groups include dairy products, eggs, animal fats, fish and seafood, poultry and red meat, and miscellaneous animal-based foods.These eighteen food groups were ranked into quintiles and given scores between 1 and 5.For making PDI, the highest quintile of a healthy and less healthy received a score of 5 and the lowest quintile received a score of 1. Participants in the highest quintile of animal food groups received a score of 1 and those to the lowest quintile received a score of 5.For hPDI, a positive score was considered for healthy plant food groups and a reverse score was considered for unhealthy plant food groups and animal food groups.For creating uPDI, a score of 1 was given to the lowest quintile of less healthy plant food groups and 5 for the highest quintile, whereas reverse scores were applied to a healthy plant and animal food groups.For calculating indices, 18 food group scores for an individual were summed, with a theoretical range of 18 (lowest possible score) to 90 (highest possible score).

Assessment of psychological health
In this study Persian version of the Beck Depression Inventory (BDI) was used for the assessment of depression.This questionnaire comprises 21 items that evaluated a range of depression symptoms including feelings of guilt, feelings of hopelessness, sadness, crying, sleep disturbance, fear and loss of appetite over the past 2 weeks.The range of these scores was between 0 and 63 points.If the BDI score was < 16, persons was considered as not and they were characterized as depressed if the subject's score was > 16.The validity and reliability of BDI were assessed in previous studies 28 .
For the assessment of health-related QoL, the SF-12v2 questionnaire was used.This questionnaire is a short form of the SF-36 questionnaire and an improved version of SF-12v1 29 .The validity and reliability of this questionnaire were approved in Iran by a previous study 30 .
We used a validated Iranian version of the Insomnia Severity Index (ISI) questionnaire for the assessment of insomnia 31 .The ISI questionnaire included seven questions.Range of scores between 0 and 4 which is stratified into four categories as follows: 0 (None), 1 (Mild), 2 (Moderate), 3 (Severe) and 4 (Very Severe).A total score of ISI ranges between 0 to 28 points.If the total score of ISI was > 7, the participants were considered to have Insomnia.

Statistical methods
Participants were divided into four groups based on quartiles of PDI; hPDI; and uPDI score.One-way-ANOVA analyses was used for comparing general characteristics and anthropometric indices of the study population across quartiles of PDI; hPDI; and uPDI scores.Univariate and multivariate regression were applied in crude and adjusted models to investigate the association quartile of PDI; hPDI; and uPDI score and poor QoL, depression and insomnia.In the adjusted models, we adjusted for age and energy intake in Model I. Additionally, adjustment was done for percentile BMI in Model II and finally, age, energy intake, BMI percentile, physical activity, menstrual status, father's job and mother's job were adjusted in the model III.All statistical analyses were performed using the SPSS v 23.P-values less than 0.05 were characterized as statistically significant.

Ethical approval and consent to participate
The ethical committee of Mashhad University of Medical Sciences approved the written informed consent (code number: 931188).The written informed consent was signed by all participants before the beginning study.

General characteristics study participants
The mean age of the participants was 14.5 years.The prevalence of depression, poor QoL and insomnia were 24%, 49% and 49.8% respectively.General characteristics and anthropometric indices of the participants across quartiles of PDI, hPDI, uPDI are demonstrated in Table 1.Level of age, scores of the QoL and insomnia were not different between quartiles of PDI, hPDI, uPDI.Participants in the lowest quartile of uPDI had lower depression scores compared the highest quartile.Percentile BMI and weight was significantly higher in the lowest adherence to uPDI than those in highest adherence to uPDI.

Dietary intake of study participants
The dietary intake of study participants across quartiles of PDI, hPDI, uPDI is shown in Table 2. Participants in the highest quartiles of PDI compared with the participants who were in the lowest quartile had higher intakes of energy, protein, carbohydrate, fat, whole grain, nuts, legumes, fruits, vegetables, vegetable oils, tea and coffee, potato, SSBs, fruit juice and refined grain.Intake of whole grain, tea and coffee and vegetable oil was significantly higher in subjects in the highest quartiles of hPDI compared with the participants who were in the lowest quartile.
Participants in the first quartile of uPDI had a higher intake of energy, carbohydrate, fat, protein, whole grain, legumes, nuts, vegetables, vegetable oil and fruit compared with the fourth quartile.

Association between food groups and depression, poor quality of life
Multi-variable adjusted odds ratios (ORs) for depression, poor QoL and insomnia categories across quartiles of food intake are represented in Table 3.There was no association between a score of PDI and poor QoL, depression and insomnia.Subjects in the highest quartile of hPDI had lower odds of insomnia compared with the subjects in the first quartile (OR: 0.50; 95% CI 0.27-0.91,P = 0.024) in the crude model.This association remained significant after adjustments for age, energy intake, BMI percentile, physical activity, menstruation, father's job, and mother's job (OR: 0.47; 95% CI 0.24-0.93,P = 0.032).However, a score of hPDI was not significantly associated with the odds of depression and poor QoL.Highest adherence to an uPDI increased the odds of depression (OR: 1.83; 95% CI 1.09-3.08,P = 0.022).There was no association between uPDI and odds of insomnia and poor QoL.

Discussion
The present study suggests that a higher hPDI score is associated with a reduced chance of insomnia.High scores on the uPDI was associated with an increased risk of depression.There is limited cross-sectional evidence on the association between PDI scores and sleep quality, mental health profile and poor QoL.In agreement with us, Haghighatdoost et al. 32 found that higher compliance with an uPDI was associated with a higher risk of depression whereas PDI and hPDI were not related to it, As Daneshzad et al. 33 and zamani et al. 34 , both reported that a high adherence of an uPDI increased risk of depression in diabetic women and healthy, respectively.Some potential explanations for the correlation between a low PDI score and an increased risk of depression could be attributed to the dietary intakes of the participants in this study.So that, those in the upper quartile had lower consumption of healthful plant foods such as whole grains, legumes, nuts, vegetables, vegetable oils and fruits, and higher consumption of unhealthy plant foods such as refined grains and SSBs, which may influence on inflammation and antioxidant levels, potentially influencing brain function 35,36 .Additionally, a potential contributing factor to the risk of depression could be the deficiency of nutrients like vitamin D and B12, typically found in animal products, while participants with higher uPDI scores in this study, reported significantly lower consumption of these types of foods, such as dairy products, eggs, fish or seafood, meat, animal fats and other animal-based foods 37,38 .However, a large cross-sectional study involving Iranian adults observed no association between the uPDI and the odds of depression 39 .Differences in findings may stem from the varied methodologies used to assess depression and dietary intake.
Our findings showed no significant link between overall PDI, hPDI, and uPDI scores and poor QoL after adjusting for all confounding factors.No study has yet evaluated the relationship between PDI indices and QoL.However, a cross-sectional, multicentric study aligns with our findings, indicating that a higher uPDI is associated with an increased risk of depression, whereas PDI and hPDI show no association with depression 32 .In this line, the Nurses' Health Study demonstrated that a decline in the quality of a plant-based diet was correlated with a lower QoL 40 .A possible explanation based on previous studies may be an increased depression due to a lower intake of antioxidant vitamins and fibers from fruits and vegetables 41,42 which may influence on QoL However, the mechanisms by which dietary quality directly influences QoL are still not fully understood; therefore, specific studies are required to assess the relationship between plant-based diet indices and QoL.
Recent research indicates a connection between diet and sleep quality.A systematic review 15 has revealed that a diet rich in healthy foods correlates with improved sleep quality, whereas a diet high in processed and sugarrich foods s associated with poorer sleep patterns.Data from a cohort study 43 suggested that individuals with poor quality of sleep consumed fewer healthful plant-based foods including fruits, vegetables, whole grains and legumes.In our population a higher adherence of the hPDI was correlated to lower odds insomnia.Consistent with our findings, Ferranti et al. reported a positive association between fruits and vegetables intake and sleep duration 44 .A healthful plant-based diet that includes foods rich in vitamins, minerals, antioxidants, phytochemicals may through to stop inflammation and to reduce oxidative stress, are related to sleep disturbances 45  www.nature.com/scientificreports/tea, coffee) and phenolic acids in (fruits, coffee, pulses, nuts) and phytoestrogens like isoflavones and lignans in (legumes), may be associated with improved sleep quality among individuals 45 .Numerous studies have demonstrated that certain flavonoids, such as apigenin and hydroxycinnamic acids, enhance cellular antioxidant defense against oxidative stress in the central nervous system 46 .Moreover, lignans have been shown to exert anti-oxidative and anti-inflammatory effects on neurons, as well as protect the blood-brain barrier from inflammatory cells by mitigating oxidative stress, inflammation, and permeability 47 .Additionally, melatonin found in legumes 48 and fruits and vegetables 49 may help alleviate sleep disorders.St-Onge et al. 50and lin et al. 51 , has indicated that fruits such as tart cherries, rich in melatonin and phytonutrients, along with kiwifruit, abundant in folate and serotonin, can potentially enhance sleep quality.Furthermore, antioxidant vitamins like vitamin C and E may bolster sleep by protecting against damaging free radicals 51 .It has been suggested that a diet high in protein could boost alertness by increasing tyrosine levels, an amino acid, and by stimulating the production of catecholamines 52,53 .This contrasts with our study population, where individuals with higher adherence to the hPDI reported lower protein consumption.Previous reviews have indicated that consuming foods rich in tryptophan, may be associated with enhanced sleep quality 54 A study has reported that the intake of tryptophan is positively correlated with sleep duration 55 .However, some research has not established a connection between tryptophan-rich healthy plant-based diets and sleep quality.This lack of association could be attributed to the suboptimal tryptophan dosage for participants, changes in sleep patterns over time, and the impact of meal timing and body composition on sleep 56,57 .This study is the first to explore the association between plant-based diet indices and conditions such as insomnia, depression, and poor QoL in Iranian adolescent girls.We used a valid and reliable FFQ to assess dietary intakes.However, this study had some limitations, such as the cross-sectional design that cannot determine causality and therefore, it required prospective studies to verify findings.Potential misclassification may occur due to measurement errors in FFQ.Our study did not account for hormonal changes as a potential confounding factor affecting mood during menstruation in girls.Additionally, it should be noted that our study population was confined to adolescent girls.

Conclusions
We have shown that higher adherence to an unhealthy plant-based diet correlates with an increased risk of depression, whereas a stronger compliance to healthy plant foods is linked to a reduced risk of insomnia.However, no significant relevance was found between PDI, hPDI, and uPDI scores and poor QoL.Nonetheless, our findings require validation through longitudinal or follow-up studies.

Figure 1 .
Figure 1.Flowchart of the data collection process of study.

Table 1 .
General characteristics and Anthropometric indices of study participants by quartiles of PDI, uPDI, hPDI.PDI: plant dietary index, uPDI: unhealthy dietary index, hPDI: healthy dietary index, BMI: body mass index, Q: Quartile, Values are means ± SD.