Injectable hydrogel with doxorubicin-loaded ZIF-8 nanoparticles for tumor postoperative treatments and wound repair

The need for tumor postoperative treatments aimed at recurrence prevention and tissue regeneration have raised wide considerations in the context of the design and functionalization of implants. Herein, an injectable hydrogel system encapsulated with anti-tumor, anti-oxidant dual functional nanoparticles has been developed in order to prevent tumor relapse after surgery and promote wound repair. The utilization of biocompatible gelatin methacryloyl (GelMA) was geared towards localized therapeutic intervention. Zeolitic imidazolate framework-8@ceric oxide (ZIF-8@CeO2, ZC) nanoparticles (NPs) were purposefully devised for their proficiency as reactive oxygen species (ROS) scavengers. Furthermore, injectable GelMA hydrogels loaded with ZC NPs carrying doxorubicin (ZC-DOX@GEL) were tailored as multifunctional postoperative implants, ensuring the efficacious eradication of residual tumor cells and alleviation of oxidative stress. In vitro and in vivo experiments were conducted to substantiate the efficacy in cancer cell elimination and the prevention of tumor recurrence through the synergistic chemotherapy approach employed with ZC-DOX@GEL. The acceleration of tissue regeneration and in vitro ROS scavenging attributes of ZC@GEL were corroborated using rat models of wound healing. The results underscore the potential of the multifaceted hydrogels presented herein for their promising application in tumor postoperative treatments.

Surgical resection stands as the prevailing modality for the treatment of diverse solid tumors.However, patients are confronted with the inherent risk of recurrent tumors and postoperative wounds 1,2 .Tumor recurrence, primarily attributed to the presence of residual tumor cells post-surgery, entails significant mortality implications 3 .Conventional chemotherapy is frequently employed to target residual tumor cells; nonetheless, its efficacy is constrained by suboptimal delivery efficiency and non-selective cytotoxicity 4 .Moreover, chronic wounds at surgical sites may be concomitant with sustained inflammation, potentially exacerbating the likelihood of tumor recurrence 3,5,6 .Hence, there is an exigency to devise a postoperative adjuvant therapy platform proficient in the efficient eradication of residual tumor cells while concurrently establishing an optimal microenvironment at tumor resection sites to expedite wound healing.
In the realm of advanced postoperative tumor treatment, a paramount objective is to attain a heightened drug concentration precisely at the target tumor resection site while mitigating systemic toxicity.Among the diverse materials employed in the formulation of nanomedicines, metal-organic frameworks (MOFs) have emerged as a pivotal category of crystalline porous materials.These frameworks are constructed through the coordination of metal ions with organic linkers and have garnered substantial attention as apt nanocarriers for drug delivery

Materials
All material details can be found in supplementary information.All experimental protocols were approved by the Animal Use and Care Committee of Tianjin Tumor Hospital.All methods were carried out in accordance with relevant guidelines and regulations.

Synthesis of GelMA hydrogel
A detailed procedure for the synthesis of GelMA is available in supplementary information.

Synthesis of ZIF-8@CeO 2 and DOX@ ZIF-8@CeO 2 nanoparticles (NPs)
Ceric oxide nanoparticles (CeO 2 NPs) were synthesized following established protocols with slight modifications 24 .Specifically, 430 mg of cerium (III) acetate hydrate and 3.2 g of oleylamine were dissolved in 15 mL of xylenes and vigorously stirred for 24 h.The mixture was then gradually heated to 90 °C, and 1 mL of deionized (DI) water was swiftly injected.The solution was maintained at 90 °C for 3 h.Ethanol was employed to precipitate the CeO 2 NPs, which were subsequently collected through centrifugation.
The synthesis of DOX@ZIF-8@ CeO 2 (ZC-DOX) followed a previously reported procedure with some adaptations 25 .Specifically, 500 mg of 2-methylimidazole and 5 mg of doxorubicin were combined in 4 mL of methanol and stirred for 10 min at room temperature.Subsequently, 1 mL of a water solution containing 2.5 mg of Zn(NO 3 ) 2 •6H 2 O was introduced to the mixture.The reaction was vigorously stirred for 15 min, resulting in an emulsion-like suspension.The nano-cerium oxide particles and polyvinylpyrrolidone were dispersed in methanol and added to the emulsion-like suspension, followed by stirring for an additional 5 min at room temperature.The final product was obtained through centrifugation, and impurities were removed by washing with methanol.ZIF-8@CeO 2 (ZC) was synthesized using the same protocol.

Structural characterization and properties tests
Scanning electron microscopy (SEM) was utilized for the observation of morphological details of ZC-DOX NPs and the intersection network and elemental mapping of the hydrogel.The structures of the ZC-DOX NPs were characterized using transmission electron microscopy (TEM, JEM2100, JEOL, Japan).
The particle sizes and zeta potentials of ZIF-8@CeO 2 and DOX@ZIF-8@CeO 2 NPs were determined via dynamic light scattering (DLS) at 25℃, utilizing a Zetasizer Nano ZS (Malvern, UK) equipped with a 633 nm laser and a detector set at a detection angle of 173°.Each sample was measured in triplicate.
To assess drug-release kinetics, 3 mL of ZC-DOX@GEL was immersed in 20 mL of PBS containing 1 mg/ mL Tween 80, maintained at 37℃ within a tube.Subsequently, 3 mL of the solution was analyzed using UV-vis spectroscopy to quantify the released drug amount, after which it was returned to the tube.
The infrared spectrum of the hydrogels was acquired by combining freeze-dried gel powders with KBr, using a Fourier transform spectrometer (FTIR, Bruck EQUINOX55) with a measurement wavelength range spanning from 4000 to 400 cm −1 .
The rheological properties of ZC-DOX@GEL hydrogel were investigated utilizing a TA rheometer (AR G2, USA).Typically, the hydrogel was positioned on a parallel-plate with a 20-mm diameter, and the modulus G'

In vitro cell experiments
Cy5-labeled DOX was employed to monitor cellular uptake using a fluorescence microscope.4T1 cells were seeded into 6-well plates at a density of 5 × 10 5 cells per well, incubated overnight, and then treated with Cy5-DOX@ZIF-8@ CeO 2 for 4 h.Subsequently, cells were washed and stained with 1 M DAPI for 30 min, followed by imaging with a fluorescence microscope (Nikon, Japan).
L929 fibroblasts and human epithelial cells (MCF-10A) were seeded into 96-well plates (1 × 10 4 cells per well) pretreated with various hydrogel groups and cultured for 1, 3, and 7 days, respectively.Subsequently, a CCK-8 assay was conducted on the cells, and absorbance at 450 nm was measured using a microplate reader.
To assess tumor cell cytotoxicity, 2 × 10 4 4T1 cells were seeded in 48-well plates with 1 mL complete DMEM medium.After 12 h of incubation, 50 μL of various hydrogels or PBS were added to the wells.After an additional 24 h of incubation, experimental groups were subjected to live/dead staining using Calcein-AM/PI.
Transwell chamber assays were performed using Transwell permeable plates (Corning, USA) consisting of poly-carbonate Transwell inserts (8 μm pore diameter) and a 24-well plate.The 4T1 cells (2 × 10 4 ) were seeded in the upper half of the insert membrane, then we implanted various hydrogel in the lower compartment of the 24-well plate and cultured for 24 h.The cells were fixed with 4% paraformaldehyde for 30 min.After the cells were rinsed with PBS, 0.1% crystal violet staining solution was used to stain them for 10 min.Images of migrating cells in the lower half of each insert were observed and captured, and the number of cells that migrated to the lower part of the insert was counted in three microscopic views per well.Subsequently, the difference in the mean cell number per well was analyzed.
For the evaluation of ROS scavenging ability and the resulting cell-protective effects, 1 × 10 5 BMDMs cells were seeded in 48-well plates with 1 mL complete DMEM medium.After 12 h of incubation, the medium was replaced with 1 mL complete DMEM medium containing 1 mM H 2 O 2 , along with 100 μL of various hydrogels or PBS.After 2 h of incubation, the hydrogels were removed, and the plates were gently washed with PBS.The cells were stained with DCFH-DA and Hoechst 33342, then observed under a fluorescence microscope.
For the quantitative analysis of the anti-inflammatory effect of these hydrogels, RT-PCR was employed.RAW264.7 cells were seeded at a density of 2 × 10 5 cells/well in 6-well plates and cultured with or without hydrogels overnight.Subsequently, they were treated with or without 0.5 μg/mL LPS for 24 h.After stimulation, we extracted total RNA by a Cell Total RNA Isolation Kit (Foregene, China), and converted total RNA to complementary DNA using 5 × RT Master Mix (Toyobo, Osaka, Japan).RT-PCR was performed on a StepOneTM Real-Time PCR system (Applied Biosystems, USA) using 2 × RealStar Green Fast mixture (Genstar, Beijing, China) according to the standard procedure.The primer sequences for IL-6, iNOS, Arg-1, and CD206 are shown in Table S1.To detect the reliability of the primers, we established fusion curves for each reaction system, and there was no nonspecific amplification in the dissolution curves.Relative mRNA expression was normalized to that of the housekeeping gene GAPDH and calculated by using the 2-ΔΔCT method.

Tumor resection and postoperative prevention
To investigate the tumor therapeutic effect of hydrogels, incomplete resection of a breast cancer model was successfully established according to a study of Tong et al. 18 with some modification.In detail, orthotopic breast cancer-bearing Balb/c mice (18-25 g, 4-6 weeks old) were established by injecting 5 × 10 5 4T1 cells into the mammary fat pad under the right breast.Tumors were surgically removed after reaching a volume of 100 mm 3 under anesthesia (leaving about 5% of the tumor mass).On the second day post-surgery, 5 × 10 5 4T1 cells incubated in 50 μL of matrigel were injected into the vicinity of the surgical sites.Subsequently, 100 μL of various hydrogels or PBS were administered to the surgical sites.For DOX@GEL group, every mouse was treated with 100 μL GelMA hydrogels containing 28.2 μg DOX.For ZC-DOX@GEL group, every mouse was treated with 100 μL ZC-DOX@ GEL hydrogels containing 100 μg ZC and 28.2 μg DOX.The mice were categorized into four groups based on different materials and phototherapeutic interventions: PBS, GEL, DOX@GEL, and ZC-DOX@GEL.Body weights and tumor volumes were assessed every 3 days.Tumor size was calculated according to the following formula: On the 21st day following postoperative treatments, the mice were humanely euthanized by neck dislocation, and their heart, liver, spleen, lung, kidney tissues were excised for further analysis.

Wound healing assays
A full-thickness excisional skin model was established in male SD rats (6-8 weeks old) to evaluate the wound healing potential of the hydrogels.Briefly, the rats were anesthetized, shaved, and cutaneous wounds were created using an 8 mm diameter biopsy punch.Subsequently, the rats were randomly assigned to one of three treatment groups (n = 3): PBS treatment alone, GelMA, and ZC@GelMA.At designated time points, skin samples from the wound area were collected and embedded in paraffin.The histopathological analyses of the samples were conducted using H&E and MassFon staining.

Statistical analysis
All the data values were expressed as the mean ± standard deviation (SD).The inter-group differences in the experiments were evaluated using a two-tailed t-test through GraphPad Prism 6 software.Statistical significance was indicated as *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001, respectively.
Tumor volume mm 3

Consent for publication
All authors have reviewed the final version of the manuscript and approved it for publication.The study is reported in accordance with ARRIVE guidelines.

Preparation and characterization of ZC-DOX@GEL
The ZC-DOX NPs were synthesized using the previously reported method 25 .They exhibited a spherical structure with a size of approximately 102 nm, as observed through scanning electron microscopy (SEM) (Fig. 2A and  C).The morphology of ZC-DOX NPs was also observed by transmission electron microscopy (TEM) (Fig. 2B), exhibiting a regular circular shape with uniform dispersed CeO 2 particles attached to its core structure surface.The zeta potential of DOX@ZIF-8@CeO 2 NPs (37.4 ± 4.7 mV) was slightly higher than that of ZIF-8@CeO 2 NPs (24.2 ± 3.7 mV), indicating the successful encapsulation of the DOX drugs (Fig. 2D).The elemental distribution in ZC-DOX@GEL was investigated using SEM (Fig. 2E) and energy dispersive spectrometry (EDS) mapping.After incorporating DOX@ZIF-8@CeO 2 MOFs, the results indicated the presence of common elements such as C, Zn, and N inherited from ZIF-8, and Ce exclusively originating from CeO 2 , as shown in the EDS mapping (Fig. 2F).In the Fourier transform infrared spectrometer (FTIR) data, the bands at 995, 1311, and 1579 cm −1 corresponded to the C-H stretching of 2-MeIM, and the band at 420 cm −1 was attributed to the Zn-N stretch (Fig. 2G).ZIF-8 NPs are pH-responsive due to the dissociation of the coordination between Zn 2+ and 2-MeIM in acidic environments 26 .This property can be utilized as a targeting mechanism, where the release of the loaded drug from ZIF-8 NPs is triggered at tumor sites owing to their relatively lower pH (acidic environment) compared to normal tissues.Furthermore, we conducted another study to determine the effect of the nanoparticles on the loading capacity of the DOX.The drug loading rate and the encapsulation rate of the ZIF8@CeO2 nanoparticles and the DOX with different mass ratios (m (NPs): m (DOX)) were explored and summarized as shown in Table (shown below), which showed that the ZIF8@CeO2 nanoparticles exhibited excellent encapsulation efficiency for the DOX (> 63.9%).When the mass ratio of m (NPs): m (DOX) was 1:0.5, the final DOX drug load efficiency reached up to 28.2% (Table 1).This proves that the encapsulation of nanoparticles is effective.
To investigate the pH-responsive behavior of ZIF-8 NPs, an in vitro release study of DOX was conducted in PBS at various pH values (pH 5.5 and pH 7.4).The release rate of DOX from DOX@ZIF-8@CeO 2 NPs in pH 7.4 PBS was only 23% of the total amount in the NPs after 32 h of incubation.However, the cumulative drug release was 63% in pH 5.5 PBS, indicating a significantly increased release rate under acidic conditions (Fig. 2H).Meanwhile, the NPs incorporated in the hydrogel demonstrated a prolonged release profile, which was beneficial for maintaining local drug concentrations at the tumor treatment site.Dynamic rheological curves (Fig. 2I,J) of the ZC-DOX@GEL sample demonstrated that G' (storage modulus) was greater than G" (loss or viscous modulus), indicating the formation of a stable hydrogel structure.

In vitro anticancer effects and cytocompatibility of ZC-DOX@GEL
The cellular uptake of ZC-DOX NPs was assessed via fluorescence microscopy in 4T1 cells.Cy5-labelled DOX (red) was encapsulated within ZIF-8 NPs instead of DOX for fluorescence detection, and the cell nuclei were stained with DAPI (blue).As depicted in Fig. 3A, the red fluorescence intensity of Cy5-DOX and Cy5-labelled ZC-DOX were notably higher than that in the Control group.Furthermore, the Cy5-DOX@ZIF-8@CeO 2 NPs group exhibited a more robust fluorescent intensity compared to the Cy5-DOX group, indicating that Cy5-DOX@ ZIF-8@CeO 2 NPs enhance cellular internalization.
Good biocompatibility is a critical factor for materials used in biomedical applications.L929 cells and MCF-10A cells were chosen to investigate the biocompatibility of the ZC-DOX@GEL hydrogel.Cytotoxicity toward these cells was evaluated using the CCK-8 assay (Fig. 3B,C).The results demonstrated that hydrogels containing ZC-DOX NPs exhibited relatively favorable cell viability.To assess the therapeutic effect of hydrogels in vitro, 4T1 cells were co-cultured with the hydrogels.Compared to the Control and GEL groups, the remarkable combined treatment effect of ZC-DOX@GEL was visually evident in the live/dead staining images.These images revealed a more intense red fluorescence, indicating a higher number of dead cells (Fig. 3D,E).Besides, we used Transwell experiments to analyze the effects of NPs containing hydrogels on the vertical migration of cancer cells.Crystal violet staining images showed that less migrated 4T1 cells were observed in the ZC-DOX@GEL group 24 h than in the control group (Fig. 3F,G).

The ROS scavenging behaviors and anti-inflammation effects of injectable hydrogels
The anti-oxidant and anti-inflammatory properties of ZC@GEL hydrogels were investigated at the cellular level.Rat BMDMs were stimulated with H 2 O 2 or LPS (lipopolysaccharide), the latter being a potent proinflammatory polysaccharide derived from the Gram-negative bacterial cell wall.The DCFH-DA probe was utilized to detect intracellular reactive oxygen species (ROS).As shown in the fluorescence images (Fig. 4A), ROS generation in H2O2-treated cells exhibited a sharp increase.However, in the presence of ZC@GEL hydrogels, a negligible green fluorescence was observed in the BMDM cells, indicating a significant reduction in cellular ROS levels.
To gain further insight into the anti-inflammatory properties of ZC@GEL hydrogel, quantitative analysis was conducted on the changes in IL-6, iNOS, Arg-1, and CD206 gene expression (Fig. 4B).The treatment with ZC@ GEL hydrogel efficiently suppressed the expression of pro-inflammatory genes (IL-6 and iNOS), while preserving the expression of Arg-1 and CD206 (Fig. 4B).

In vivo tumor recurrence prevention by ZC-DOX@GEL
Based on our in vitro experiments, we administered postoperative treatments of DC-DOX@GEL to Balb/c mice with breast tumors.Following surgery, all mice experienced temporary weight loss, noted on the third day (Fig. 5A), attributed to surgical trauma.Subsequently, the body weights of mice in various treatment groups increased, indicating overall health improvement.Throughout the treatment duration, variations in tumor volume among the groups were observed (Fig. 5B).The PBS and GelMA hydrogel groups showed moderate tumor growth over the testing period.In contrast, both the DOX@GEL group and the ZC-DOX@GEL group Table 1.Encapsulation rate and drug loading rate analysis of nanoparticles.Data were displayed as mean ± SD (n = 3).SD = 0.05.exhibited slight tumor growth, with the ZC-DOX@GEL group demonstrating a more pronounced therapeutic effect (Fig. 5C).After 21 days of observation and measurement, mice from all three groups were euthanized, and tissue samples were collected.Photographs of excised tumors are depicted in Fig. 5D, corroborating in vitro assay results and confirming that ZC-DOX NPs can partially inhibit tumor growth with reduced toxicity and enhanced efficacy compared to DOX alone.The in vivo safety of DC-DOX@GEL was further assessed through H&E staining (Fig. 5E) of major organs 24 h after postoperative treatments.No discernible damage was observed in vital organs (heart, liver, spleen, lungs, and kidneys), indicating minimal toxicity associated with DC-DOX@ GEL treatment.

Wound healing effects of ZC-DOX@GEL
A full-thickness excisional skin model in rats was established to simulate the wound healing process post-surgery in vivo.The mice were divided into three groups: Control (treated with PBS only), GEL (treated with GelMA hydrogel only), and ZC@GEL (treated with ZIF-8@CeO 2 -loaded GelMA hydrogel).On predetermined days, the wounded areas were photographed and the changes in the wounds were recorded.The images displayed varying degrees of wound healing in the three groups (Fig. 6A and B).Statistically, the hydrogel-treated wounds exhibited significantly faster healing compared to the control group over time.Specifically, the ZC@GelMA group demonstrated the most robust healing results, with wound closure area reaching nearly 90% after 12 days.The wound closure for the control and GEL groups achieved 76.3% and 82.38%, respectively.These blended hydrogels exhibited outstanding wound healing behavior.
To further investigate the wound closure performance of the hydrogels, H&E staining was employed.Histological analysis of the wound sites revealed varying degrees of healing in these groups after treatment for 13 days (Fig. 6C,D).Compared with the other groups, the ZC@GelMA group showed superior repair effects, with epithelial and connective tissues exhibiting a more regular structure and the presence of hair follicles on the wound sites.At higher magnification, the overall organization of the skin appeared basically normal in the ZC@GelMA group, indicating a clear structure of the stratum corneum.Additionally, abundant collagen deposition in the GelMA and DC@GelMA groups indicated satisfactory skin repair.These results collectively suggest that the mitigation of inflammation and accelerated wound healing can be achieved with DC@GEL treatment.

Discussion
Preventing tumor recurrence and promoting wound healing are paramount concerns following surgical resection.Injectable hydrogels hold great promise as matrices and multifunctional platforms for the immediate elimination of residual tumor cells and as long-term aids in wound healing.Additionally, various bioactive hydrogels have been designed to enhance tissue regeneration by providing physical or chemical cues 26 .Hydrogels endowed with the capacity to scavenge reactive oxygen species (ROS) play a crucial role in tissue regeneration.They shield cells from oxidative stress and facilitate the polarization of macrophage cells towards the M2 phenotype [27][28][29] .Despite considerable efforts to enhance anticancer efficacy through multimodal therapies and to foster wound healing via anti-inflammatory strategies, there remains a need for an adaptable matrix that supports tissues and ensures continuous ROS scavenging for improved tumor postoperative treatment.
In this study, we engineered ZIF-8@CeO 2 nanoparticles loaded with DOX.The presence of both the ZIF-8 shell and CeO 2 components within the nanoparticles ensured that the integrated drug delivery system exhibited a higher affinity towards cells and could respond effectively to the acidic microenvironment of tumors.The pathway for DOX diffusion from ZC-DOX@GEL involved a drug-containing core and the hydrogel matrix.Prior to being released from the spaces between GelMA chains, DOX had to traverse the network composed of water-filled pores in the ZIF-8@CeO 2 nanoparticle.This multi-faceted diffusion mechanism endowed ZC-DOX@ GEL with a slower initial release, thereby mitigating the issue of burst release commonly observed in free drugloaded hydrogels.Furthermore, it has been established that nanomedicines integrated into hydrogels extend the retention time of drugs in the vicinity of tumors 30,31 .Our release experiments demonstrated that ZC-DOX@GEL exhibited a gradual initial burst followed by sustained DOX release.This drug release profile aligns well with the requirements for effective tumor postoperative treatments.www.nature.com/scientificreports/Furthermore, zinc ions released from ZIF-8@CeO 2 nanoparticle might play a crucial role in regulating cellular antioxidant defense mechanisms.They participate in various mechanisms within cells to maintain the balance of oxidation-reduction processes and intra microenvironment stability, thus aiding in the neutralization of harmful ROS 32,33 .Specifically, zinc ions interact with antioxidant enzymes such as superoxide dismutase and catalase, as well as metallothionein, enhancing their activity and stability 34 .These enzymes and molecules work to eliminate free radicals and other detrimental oxidative substances, thereby reducing cellular damage caused by oxidative stress.Additionally, zinc ions influence intracellular redox reactions, modulating the cellular oxidation-reduction state.They form stable complexes with thiols (sulfur-containing molecules) and proteins, thereby affecting the cellular redox balance 35 .By regulating redox reactions, zinc ions reduce the production of free radicals within cells, preventing cellular damage induced by oxidative stress.Therefore, zinc ions might play a pivotal role in cellular antioxidant defense by enhancing antioxidant enzyme activity, regulating redox reactions, and maintaining intracellular redox balance.Maintaining appropriate levels by controlled release of zinc ions from ZIF-8@CeO 2 nanoparticle is essential for cellular antioxidant defense and would healing.
The rheological analysis (Fg.2I and J) consistently demonstrated solid-like elastic behavior, a characteristic trait of hydrogels, as indicated by the storage modulus (G') surpassing the loss modulus (G'') across the frequency range 36,37 .The injectability of our fabricated hydrogels stems from their specific shear-thinning behavior.The incorporation of ZC-DOX NPs in the hydrogel matrix provides a reservoir for chemotherapeutic drugs, such www.nature.com/scientificreports/as DOX, enabling efficient tumor elimination (Fig. 3D).Moreover, ROS, generated in wounds due to bacterial infection or by normal cells at the wound site, can accumulate excessively, triggering an inflammatory response that impedes tissue repair and damages healthy cells 38,39 .The CeO 2 component exhibits CAT-like and SOD-like catalytic activities, along with the ability to scavenge hydroxyl radicals in the hydrogel system, ensuring sustained ROS scavenging (Fig. 4A and B) 40,41 .Excessive levels of ROS can lead to elevated levels of inflammation through directly modifying signaling proteins or by inducing oxidative stress and then lead to the maturation and secretion of pro-inflammatory cytokines such as interleukin-1β (IL-1β) and interleukin-18 (IL-18) 42,43 .Additionally, excessive ROS can induce the expression of pro-inflammatory genes by activating transcription factors such as NF-κB and activator protein-1 (AP-1) 44,45 .These transcription factors bind to the promoters of pro-inflammatory genes and stimulate their transcription, leading to the production of cytokines, chemokines, and adhesion molecules that recruit immune cells to the site of inflammation 46,47 .The relationship between inflammation and wound repair is a complex and dynamic process that involves various cellular and molecular interactions 38,48,49 .When tissue damage occurs, inflammatory cells such as neutrophils and macrophages are recruited to the site of injury, where they release cytokines and chemokines, promoting inflammation and attracting other immune cells to clear debris and pathogens from the wound area, thereby initiating a crucial initial inflammatory response 50 .Inflammatory cells release growth factors and cytokines that stimulate various aspects of the wound healing process.For example, transforming growth factorbeta (TGF-β) is released by macrophages and plays a central role in promoting the proliferation of fibroblasts and the synthesis of extracellular matrix proteins such as collagen 51 .Additionally, inflammatory cytokines such as interleukin-1 (IL-1) and tumor necrosis factor-alpha (TNF-α) stimulate the migration of keratinocytes and fibroblasts to the wound site 52 .However, failure to resolve inflammation can lead to chronic inflammation and impaired wound healing 53 .As the wound healing process progresses, anti-inflammatory signals are released to dampen the inflammatory response and promote tissue repair.Macrophages undergo a phenotypic switch from a pro-inflammatory (M1) phenotype to an anti-inflammatory (M2) phenotype, which promotes tissue remodeling and angiogenesis 54 .Additionally, inflammation also promotes angiogenesis, the formation of new blood vessels, which is essential for delivering oxygen and nutrients to the wound site 55 .
Both in vivo and in vitro experiments unequivocally confirmed the prevention of tumor recurrence and the acceleration of wound healing.Local chemotherapy administration demonstrated remarkable anticancer effects, leading to complete elimination of residual tumors.Furthermore, the injectable hydrogels induced a notably accelerated wound healing process compared to commercial wound dressings (Fig. 6).Hence, these multifunctional platforms, based on injectable hydrogels integrating multi-modal cancer therapies and ROS scavenging, present themselves as promising candidates for postoperative tumor treatments.

Conclusion
In conclusion, we have successfully developed an inflammation-responsive injectable hydrogel system loaded with DOX, demonstrating potent localized tumor-suppressive and wound-healing effects.The notable antitumor efficacy of the composite hydrogels arises from the pH-sensitive release of DOX precisely at tumor sites.Concurrently, these composite hydrogels exhibit remarkable capabilities for efficient wound repair, achieved through the alleviation of wound-related inflammation and the creation of a conducive reparative microenvironment.Collectively, these findings offer valuable insights for the advancement of a postoperative adjuvant platform, effectively addressing clinical challenges associated with surgery, including wound healing post-resection and the prevention of tumor recurrence.This work not only introduces a valuable adjunct for postoperative therapy but also presents a straightforward methodology for synergistically harnessing diverse substances to achieve a myriad of biological applications.

Figure 3 .
Figure 3.In vitro anticancer activity tests.(A) CLSM images of cellular uptake of DOX and DOX@ZIF8@ CeO 2 .(B) Cell viability of L929 with different treatments.(C) Cell viability of MCF-10A with different treatments.(D) Live/dead 4T1 cell viability evaluation of Control, GEL, ZC@GEL and ZC-DOX@GEL.(E) Quantitatively analysis of 4T1 cell viability.(F) Crystal violet staining of the migrated 4T1 cell.(G) Quantitatively analysis of migrated 4T1 cell in different groups (n = 3).

Figure 5 .
Figure 5.In vivo anticancer effect to 4T1 bearing balb/c mice (n = 4).(A) Body weight of 4T1 bearing balb/c mice as a function of time after different treatments.(B) H&E staining of major organs of balb/c mice treated with PBS or DZCG hydrogel, respectively.(C) Tumor volume of 4T1 bearing balb/c mice as a function of time after different treatments.(D) Tumor weights of 4T1 tumor-bearing mice with various treatments.(E) Photographs of the excised tumors.

Figure 6 .
Figure 6.Wound healing ability.(A) Photography of representative wounds at different time after different treatments (scale bar: 5 mm).(B) Quantitative evaluation of wound healing ratio as a function of time after treatment.H&E (C) and Masson (D) staining of healing wounds from rat 13 days received different treatments, respectively (scale bar: 100 μm.
= width 2 × length/2.All experimental protocols were approved by the Animal Use and Care Committee of Tianjin Tumor Hospital.