Knowledge and handling practices for raw hen's eggs during purchase, preparation, storage, and consumption: a cross sectional study

The study aimed to assess the hen’s eggs knowledge, handling practices, and consumption among Palestinian consumers using the cross-sectional survey design. Online questionnaire was sent via social media platforms including well-known Facebook and WhatsApp groups in West bank, Gaza and Jerusalem, along with an invitation explaining the research objectives and the voluntary participation. The online questionnaire consisted of three main sections: socio demographic, knowledge and practices for hen’s eggs during purchase, preparation, storage, and consumption. The items were developed based on previous literature and international guidelines for hen's eggs purchase and handling, and subjected to content validity process, followed by a pilot study to determine the reliability of the questionnaire using Cronbach’s alpha test. The final data were analysed based on the dichotomous Rach model for knowledge and polytomous Rasch model for practices using Winsteps version 5.1.3. The Rasch SPSS output interval data files were used in the inferential analyses tests (one-way ANOVA test and independent samples t-test). The Rasch analysis showed that on average the participants had quite low level of knowledge of hen's eggs consumption safety, (person ability Mean 0.10 logit). The item difficulty measures ranged between + 1.65 (most difficult) and − 2.24 logit (easiest). It was not easy for the participants to endorse the items pertaining their practices in handling the hen’s eggs during purchase, preparation, storage and consumption, (person ability Mean − 0.11 logit). The item difficulty measures ranged between + 2.68 logits (most difficult) to − 2.45 logit (easiest). In addition, female participants significantly outperformed males in knowledge scores; and the level of hen's eggs hygiene practices and storage during purchase depended on participants’ knowledge level. The research recommended interventional programs to enhance Palestinians’ awareness and knowledge about hen's eggs knowledge, handling practices, and consumption. Further quantitative and qualitative research studies were also recommended.


Factors influencing egg quality and consumer habits
Consumer habits for specific hen's eggs characteristics vary from place to place.Many prefer farm hen's eggs than industrial hen's eggs as they think that they are more flavorful with better texture and color 7 .
Literature has reported abnormalities that can be attributed to hen's age-when older hens lay eggs with bad quality-or may be due to certain diseases, changes in their hormones, certain medications, and stress 4 .Moreover, low level of calcium in the hens' feed result in reduction in egg shell quality, egg size, shell thickness, and cracked shells 4 .Improper food handling practices as storing temperature, time, and cross contamination are associated with food borne disease 6 .Improper storage can accelerate bacterial growth, potentially compromising the integrity of the hen's eggs and increasing the likelihood of foodborne illnesses.
Hen's eggs borne human Salmonellosis can be explained by the contamination of the egg shell with Salmonella or its ability to gain entrance inside the egg during egg formation in hens' utero 8 .When egg shells contaminated by fecal or environmental Salmonella, Salmonella can penetrate inside the egg through the pores present in the egg shell 8 .The risk of Salmonella enteritis in hen's eggs increased in poor refrigeration, consumption of raw hen's eggs and time and temperature abuse 9 .As a result of salmonellosis, patients experience diarrhea, fever, abdominal pain or cramps, vomiting, headaches, and nausea.Incubation lasts between 8 and 72 h.Symptoms can last up to a week.Salmonella infections vary from mild to severe and are occasionally fatal.Other bacterial infections such as E. coli and Campylobacter are also possible health risks associated with hen's eggs consumption, emphasizing the critical importance of proper handling, storage, and cooking to mitigate these health risks.
According to United States Department of Agriculture (USDA) regulations, only intact shell eggs are allowed to be marketed to consumers, while cracked eggs may be processed to dry egg products 10 .For shell egg cleanliness, USDA guideline requires shell eggs washing 8 .
Numerous studies have assessed consumers' knowledge and practices related to the handling of chicken meat and eggs 2,3,6 .In the Palestinian context, published studies examining consumers' knowledge and practices regarding hen's eggs safety are scarce.Additionally, Palestine's unique geopolitical situation and specific challenges related to food security and access may impact consumer perceptions of food safety.These factors are expected to play a critical role in shaping the understanding of hen's eggs safety among consumers in Palestine, warranting a specific examination in this context.Thus, the purpose of this study was to determine participants' knowledge regarding hen's eggs safe purchase practices, storage, and consumption practices.It also aims to examine the differences in knowledge levels among various consumers' groups and characteristics, as well as the relationship between knowledge levels and practices when it comes to hen's eggs purchase, storage, and consumption.Understanding consumer behavior and knowledge gaps is vital for food safety and public health.Given the rising concern for foodborne illnesses, this study is essential for safeguarding the well-being of Palestinians.Raising awareness about hen's eggs safety and proper handling can empower individuals to make informed choices and minimize the risk of diseases.Additionally, our research has practical implications for policymakers and regulatory agencies, facilitating the development of more effective guidelines to ensure hen's eggs product quality and safety.This study serves as a key resource for implementing evidence-based strategies to enhance food safety and promote better hygiene practices for the Palestinian community.

Study design and settings
The current cross sectional online survey was conducted in West Bank, Gaza, and Jerusalem in Palestine.The study covered nine governorates: Nablus, Jenin, Tolkarem, and Qalqiliah in the north of West Bank; RamAllah, Jerusalem, and Bethlehem in the middle; Hebron in the south, and Gaza.The data were collected online using Google forms due to COVID-19 pandemic, and the link was shared on social media platforms such as wellknown and large Facebook groups and popular WhatsApp groups.The participants were provided with a data collection link, which included an invitation statement outlining the study's objectives and emphasizing the voluntary nature of participation.Following this, participants were presented with an item asking if they were willing to participate in the survey, with response options of "Yes" or "No".Those who selected "Yes" were subsequently directed to the subsequent sections.The data collection period lasted one month, started in May

Questionnaire development
The questionnaire items were developed using data from the literature regarding hen's eggs safety behaviors, handling practices, preparation, and storage 3,6,11 , in addition to the European Commission's regulations and standards (EC) No. 589/2008 5 and national regulations, and Palestinian Standards Institutions 12 .The research team developed the initial draft of the questionnaire, which consisted of four sections: Sect."Introduction" contained four questions about hen's eggs purchase, Sect."Methods" contained fifteen questions on consumer knowledge of hen's eggs handling, storage, and preparation, Sect."Results" contained fifteen questions on consumer practices of hen's eggs handling, storage, and preparation, and Sect."Discussion" contained six questions about consumer perceptions of hen's eggs quality in local markets.Each section of the questionnaire was written in the participant's native language (i.e., the Arabic language).Five experts in assessment and food safety verified and approved the questionnaire's content validity.They recommended eliminating two items from the knowledge section and rewording three items in the practices section to improve clarity.All the reviewers' suggestions and corrections were taken into account, and the final questionnaire was modified accordingly.A pilot study with 45 subjects was conducted to evaluate the questionnaire's internal reliability using SPSS™ version 21.Cronbach's alpha coefficients were 0.85 and 0.75 for knowledge section and practices section respectively, indicating that the items had an acceptable level of internal consistency.The responses that were collected in the pilot study were subsequently eliminated from the analysis of the main study.The knowledge items were presented as statements, and subjects were required to indicate whether each statement was "true, " "false, " or "I don't know." The responses were coded as follows during the analysis phase: 1 point for the correct response and 0 point for the incorrect or "I don't know" response.The sum of the answers was calculated to determine the subject's overall score out of thirteen.Whereas the practices section consisted of fifteen items with three-point Likert scale: always, occasionally, or never.

Data analysis
The final collected data were analysed based on the dichotomous Rasch model (i.e.knowledge with 0, 1) and polytomous Rasch model (i.e.practices with a 3-point scale) using Winsteps version 5.1.3 13.The Rasch model for measurement was utilised because it enabled researchers to get the difficulty measure for each item and ability measure for each participant and place them on the same interval scale to see their distributions.The most difficult items to answer (knowledge) or endorse (practices) and most able participants are positioned toward the upper part of the scale and vice versa 13,14 .In addition, the statistical package for the social sciences SPSS version 21 used the Rasch output interval data files to conduct the inferential analyses (One-way analysis of variance (ANOVA) and Independent samples t-test) comparing mean scores of the knowledge construct across certain demographic variables (e.g., gender).All the results were displayed in Tables and Figures. Vol

Results
This study aimed to determine Palestinian consumers' knowledge level based on their responses to items on raw hen's eggs, and to identify their practices in handling these hen's eggs during purchase, preparation, storage and consumption.The Rasch model analyses showed the overall levels for knowledge and practices, and which knowledge items the participants got most correct and which practices items they highly endorsed.These results were followed by the inferential analysis using the interval data produced by the Rasch Model.

Participants' recruitment
A total of 620 participants responded to the online invitation and signed the online consent form.A total of 577 participants (88 Males and 489 Females) included in the final analysis because forty three participants were excluded due to their age (< 18 years old), never eat or buy hen's eggs, and missing data.

Participants' sociodemographic variables
Participants' characteristics are summarized in

Participants' knowledge of hen's eggs consumption safety
The Rasch analysis showed that on average, the participants were marginally/slightly more able than the item difficulty since the person ability mean was 0.10 logit, higher than the mean of item difficulty 0.0 logit as shown in Fig. 1 and Table 2, indicating quite low level of knowledge of hen's eggs consumption safety hen's eggs.More specifically, the item map (Fig. 1) shows that the person ability measures spanned about 7.29 logits (from − 4.37 to + 2.92) while item difficulty measures spread was about 3.89 logits (from − 2.24 to + 1.65).It also clearly displays the distribution/hierarchy of all items on one interval scale.The mostly corrected items are placed towards the lower part, and the least corrected ones are placed towards the upper part of the scale.The most difficult items for the participants to answer were item 4 (The shelf-life of the hen's eggs is 10-14 days at room temperature, 1.64 logit); item 10 (The safety of hen's eggs consumption is not affected by the presence of farm wastes on the shells,1.06logit) ; item 1(The color of the hen's eggs yolk is affected by the beta carotene content, and the yellow color increases when beta-carotene increases, 0.98 logit); item 8 (It is recommended to wash hen's eggs with water before using it to get rid of bacteria on their shells, 0.80); item 11 (The presence of blood spots inside the hen's eggs means bad storage

Practices
Overall, the Rasch analysis revealed that it was not easy for the participants to endorse or agree on the items pertaining their practices in handling the raw hen's eggs during purchase, preparation, storage and consumption as shown in (Fig. 2 and Table 3).The Mean of participants' ability was (− 0.11 logit), lower than the Mean of item difficulty (0.0 logit).The Item-Map (Fig. 2) shows that the most difficult item to be endorsed by the participants was item 15 (I eat raw hen's eggs, 2.68 logits); followed by item 3 (I buy hen's eggs from farms only, 1.08 logit); item 14 (I eat half-cooked (i.e.still semi liquid) hen's eggs, 0.94 logits); item 1 (I buy hen's eggs only if the expiry date is printed on the hen's eggs box, 0.80 logits); item 12 (I check the expiry date of hen's eggs when I use, 0.78 logits); item 5 (I buy the hen's eggs if there is chicken feces or blood spot on the eggshells), 0.76 logits); item 7 (I clean hen's eggs with a brush if they have obvious dirty on the shells, 0.49 logits); item 9 (I used the hen's eggs if the shell is broken or cracked, 0.34 logits); and item 6 (I wash the hen's eggs when I buy it if it are dirty, 0.10 logits).On the other hand, the easiest items to be endorsed by the participants were items 10 (I store the hen's eggs in the fridge when I buy, − 2.45 logit); item 11 (I wash my hands after I touch or handle the hen's egg, − 1.56 logits); item 13 (When you eat the hen's egg, you eat it after it is perfectly cooked (solid)., − 1.45 logit); item 2 (I don't use hen's eggs when it is expired., − 1.36 logit); item 4 (I buy the hen's eggs only if I confident about the grocery and storage temperature, − 0.89 logits); and item 8 (I wash the hen's eggs just before using it if it is dirty, − 0.27 logits).It seems that the participants are not so concerned about checking the expiry dates or dirtiness of the hen's eggs when they purchase hen's eggs.However, they were very particular about being clean when preparing or cooking hen's eggs and the place where they buy the hen's eggs and proper storage.

Relationship between consumers' knowledge of hen's egg safety with selected variables
The relationship between consumers' knowledge and sociodemographic variables The sociodemographic variables and their effect on consumers' knowledge were reported in Table 4.The results demonstrated that there were no statistically different effects according to the areas, marital status, educational level, work status and income in the consumers' knowledge and practices.Whereas, participants gender showed significant differences p < 0.05 in terms of knowledge scores, where females had significantly high knowledge scores as compared to males' , p < 0.05 (Table 4).

The relationship between participants' knowledge and hen's egg purchase variables
For the hen's eggs purchase practices, Table 5 shows there were no significant differences in knowledge scores for the item related to involvement in buying hen's egg.However, the participants who usually buy the hen's eggs for their family scored lower than participants who sometimes do or they don't do.Moreover, consumers who purchased hen's eggs had higher knowledge regarding hen's eggs quality and safety.

The relationship between consumers' knowledge and practices variables
Table 6 illustrates the relationship between consumers' knowledge and practices.The results revealed there a was significant difference p < 0.05 in terms of knowledge scores with the item (I clean the hen's eggs with a brush if they have obvious dirt on the shells); participants who usually do or sometimes do scored higher scores as compared to participants who do not do.

Discussion
The current study was performed originally to assess consumers' knowledge and practices regarding hen's eggs handling practices during purchase, preparation, storage and consumption, and to verify whether consumers' knowledge is correlated with their practices.Based on the current literature, this is the first research of this kind conducted in Palestine.Therefore it provides essential information to propose other interventional and educational programs in this field.www.nature.com/scientificreports/

Knowledge about hen's egg safety
The reported results showed that only 32.8% of the participants knew that the hen's egg yolk color changes according to the concentration of beta-carotene in their feeds.Roberts (2019) reported that variation in yolk color is due to the source of the pigmentation (natural or synthetic) and their levels of utilization and combinations between xanthophylls, stability and availability of xanthophylls, feed composition, stress, genetics, health status, as well as other factors 4 .This lack of knowledge potentially indicates a limited awareness of the essential nutrients present in hen's eggs and their potential health benefits.Most respondents (67.6%) declared that old hen's eggs become more watery than fresh hen's eggs.Moreover, the highest participants (71.8%) declared that hen's egg fluidity increases when it is stored in a wrong way.This reflects that most of the participants used to cook or utilize hen's eggs.A total of 40.7% of participants were aware that hen's eggs last 10-14 days at room temperature, www.nature.com/scientificreports/while 55.1% knew their shelf-life is up to 28 days from laying.Only 65 participants routinely checked hen's egg expiry dates.Moreover, 65.3% recognized the risk of Salmonella on eggshells, and 87.9% believed hen's egg quality depends on the hen's diet.Additionally, 36.5% recommended washing hen's eggs before use.The Food Agriculture Organization Food and Agriculture Organization (FAO) conducted a survey with Palestinian consumers to determine their knowledge and practices related to the consumption of hen's eggs and showed that 48.4% respondents wash hen's eggs before cracking hen's eggs shells 15 .In the current study, only 39.9% of respondents believed that the presence of cracks on eggshells indicates that eggs are not fit for consumption.
It is crucial to handle hen's eggs with care and to discard those with extensive cracks, minor hairline cracks do not necessarily render the hen's eggs unsafe for consumption if they are used promptly and properly cooked.Moreover, 31.2% and 41.1% of the participants considered the presence of farm wastes on the hen's egg shells and egg box cartons do not affect hen's eggs safety, respectively.Farm waste poses a serious risk to food safety because it can include dangerous bacteria and diseases that can infect eggs.Ignoring the possible effects of farm waste on the safety of hen's eggs could result in incorrect handling procedures, which could expose consumers to foodborne illnesses.Promoting a thorough awareness of the value of hygiene measures in guaranteeing the safety and quality of hen's eggs requires educating customers about the significance of maintaining cleanliness throughout the hen's egg production and distribution process.In order to lower the risk of foodborne illnesses and provide a safer environment for food consumption, it is important to emphasize the importance of good sanitation practices on farms and during packaging.Surprisingly, just 37.6% of participants knew that blood spots in hen's eggs suggest poor storage.However, 82% were aware of the necessary boiling time to make hen's eggs safe to eat.The survey showed that awareness of hen's egg safety is lower than anticipated, raising questions about the causes of this knowledge gap.This finding goes with 16 who found out that in Australia there was a lack of knowledge surrounding the risks of handling and using hen's eggs collected from domestic poultry.
Gender differences are found in various studies.For instance, a study conducted in the United States of young adults (mean age 19.9) demonstrated that females significantly identified safer food handling practices compared to males 17 .The gender difference in hand washing behavior when handling raw hen's eggs is particularly interesting, since several previous studies have reported that women wash their hands differently from men 18,19 ; indicating that food preparation is a task to be conducted by females in the household.Other studies conducted in Korea and Thailand, surveying 100 participants from each country, investigated hen's egg purchase, storage, and preparation practices.They discovered that female respondents were more likely to wash hen's eggs using soap compared to their male counterparts 20 .

Storage and handling practices
In this study, several noteworthy findings emerged concerning participants' hen's egg purchasing habits and their corresponding levels of knowledge about hen's egg quality and safety.The study found that regular hen's egg buyers for their families scored slightly lower on knowledge about hen's egg quality and safety than those who buy hen's eggs less often or not at all, although the difference was not statistically significant.These results were reported by 20 .Increasing knowledge regarding feeding and methods of production significantly affect hen's egg purchase 3 .The hen's egg source does not affect consumers' knowledge.The majority of respondents (368) answered that they buy hen's eggs from neighboring groceries.This finding was similar to the results obtained by Hessel et al., (2019), where 95.3% of respondents bought hen's eggs from local markets and greengrocers.However, 354 participants buy 20-30 hen's eggs each time, implying that they highly consume hen's eggs 6 .
According to the findings of the present study, 331 participants never check the expiry date on hen's eggs when they purchase; while only 67 individuals locate the expiry date on the hen's egg box.It is important to mention that according to the mandatory technical instruction of hen's egg marketing number 30-2011 by Palestinian Standard Institution (PSI), it is recommended that each hen's egg box should display the expiry date 12 .Regarding the question "I don't use hen's eggs when it is expired" 414 responded as always.The lack of small hen's egg producers and difficulty in locating producers in some cities may explain why 350 participants never buy hen's www.nature.com/scientificreports/eggs directly from the producers.309 participants buy hen's eggs when they are confident about the grocery and the storage temperature.It is observed that in Palestine as well as many other countries such as India, hen's eggs are sold at room temperature because local grocery stores do not have enough cold storage facilities 21 .
Only 298 respondents said that they never buy hen's eggs if there are chicken feces or blood spots on the eggshell; while 233 said sometimes buy hen's eggs with the presence of chicken feces or blood spots on the shell.This indicates that the consumers' knowledge regarding the safety is low.One recommendation by PSI asserts that raw hen's eggs should be free from any abnormalities, chicken residue or blood spot 4,12 Regarding the washing hen's eggs, 167 participants always wash eggs if they are dirty, 177 sometimes wash eggs, while 232 never do that.As stated in the Commission Regulation (EC) No 589/2008 5 , it is not recommended to wash Class A eggs due to the potential damage to the physical barriers, including cuticle, caused by washing 5 .Furthermore, eggs  22 .Dirty eggs should not be sold for raw consumption but can be used for processing or animal feed.The USDA recommends not washing raw eggs to prevent cross-contamination and foodborne illnesses 23 .Many food safety guidelines mention that the inner contents of the hen's egg can shrink if the wash water is unsuitable.As a result, wash water and pathogens might be drawn into the egg through the shell pores 8 .Washing dirty hen's eggs can burden egg washing systems and, if done, should follow best practices: wash immediately after collection, discard very dirty eggs, use water at 41-44 °C with a pH ≥ 10.5 for sanitizers, avoid soaking during washing, and always dry eggs afterwards to reduce the risk of microbial ingress 22,24 .

Practices in cleaning and washing hen's eggs
The study found a significant relationship (p < 0.05) between participant knowledge and the practice of cleaning hen's eggs with a brush, with most (290) never or sometimes using this method, potentially indicating a lack of awareness about effective hen's egg cleaning techniques such as brushing, which can remove contaminants and reduce hazards.It is interesting to report that a big number wash dirty hen's eggs before use as recommended by 15 FAO 2017 to clean eggs shells with soap and water before cooking them 25 .
The survey revealed that while 248 participants safely never use broken or cracked hen's eggs, 325 do use them always or sometimes, reflecting inadequate knowledge on proper hen's egg usage practices.This lack of awareness could explain why consumers often find such hen's eggs in purchased cartons, differing from an Australian study where only three participants used cracked hen's eggs 16 .The broken or cracked hen's eggs should be excluded from the uncracked eggshells.Hen's eggs that have cracked shells are extremely prone to contamination by bacteria, thereby reducing the quality and posing health hazards to consumers.Cracked or broken hen's eggs should not be sold 26 .In addition, bacteria can enter hen's eggs contents when they are broken 24 .
A considerable number of respondents (505) store hen's eggs at home in their refrigerators, showing a correct safety practice.To prevent the growth of pathogenic bacteria such as Salmonella, spp present in hen's eggs, eggs can be stored below 8 °C5,15 .Recently, Sabbah and Al-Asmar 27 , evaluated the quality and safety of the raw hen's eggs in the north Palestinian market, and they concluded that Escherichia coli contamination was detected on eggshells sourced from the Jenin governorate.Concurrently, eggshells from the Tulkarm governorate were found to be contaminated with Salmonella enteritidis.This study conclusively demonstrates the absence of essential sanitary practices such as grading, cleaning, and cooling prior to distribution.This neglect poses a potential microbiological health risk to consumers.
The absence of cold storage facilities in local grocery stores is a significant factor that impacts hen's egg safety in the Palestinian context.Hen's eggs storage may be expose to risk in the absence of sufficient refrigeration, which raises the possibility of bacterial growth and contamination.Of the survey respondents, 422 always wash their hands after handling hen's eggs, reducing the spread of potential microbes from the egg surfaces to the kitchen or ready-to-eat foods.Shell contamination may also stem from nesting materials, dust, feed, containers, and contact with humans and animals 6 .
Challenges and local climate may increase the risk of bacterial contamination and spoilage of hen's eggs.A small proportion of participants (65) always check hen's egg expiry dates, while many (322) never do, indicating limited awareness of food shelf-life.Additionally, most participants (422) eat well-cooked hen's eggs, whereas fewer (63) eat them half-cooked, and only a few (10) consume them raw.The survey conducted by FAO showed that 76.6% respondents adhered to correct practices-i.e., they cook the hen's egg until the yolk is completely solid.As mentioned by 23 that no one should eat foods containing raw hen's eggs.Pasteurized hen's eggs can, however, be eaten without cooking.
Based on the findings, to improve hen's egg safety, local stores must prioritize cold storage to lessen contamination risks, and stringent quality control must be enforced throughout hen's egg production and distribution.To ensure safer hen's eggs consumption, education campaigns need to highlight proper handling, storage, and cooking.Practical tips can help consumers make informed choices and foster better hygiene practices.Collaborative efforts from government, community, and food industry stakeholders are essential to promote consumer education and enforce safety measures for improved public health in Palestine.

Conclusion
The survey results show participants lack knowledge about hen's egg safety practices.They easily understood the nutritional value and cooking time for hen's eggs but struggled with safety-related questions.Storing hen's eggs in the fridge was simple for them, but eating raw hen's eggs was challenging.There were no major differences in hen's egg safety knowledge across sociodemographic groups, except between genders.The study identified the relationship between consumer's knowledge and practices variables showed significant differences p < 0.05; different knowledge regarding the cleaning practices of hen's eggs, eating the raw hen's eggs without good handling or suitable heat treatment.The most participants involved in this survey bought about 20-30 hen's eggs each time from the neighboring groceries.Many participants used cracked or dirty hen's eggs, against national and international standards.Unsafe practices, like storing dirty hen's eggs with ready-to-eat foods, risk cross-contamination.The study found significant discrepancies between actual hen's egg handling, purchasing, and storage practices and the safety guidelines set by PSI, FDA, and USDA.Hen's eggs can harbor dangerous pathogens like Salmonella, which can cause serious illness or even be fatal, particularly for vulnerable populations such as the elderly, infants, pregnant women, and immunocompromised individuals.Understanding where the knowledge gaps and unsafe practices exist can lead to targeted education and interventions to prevent outbreaks.It is recommended to increase the consumer awareness regarding the best method for purchasing, storage and handling the hen's eggs.Wherefore, Arabic and English booklet and public seminars was prepared by the authors :.(1234567890)
Vol.:(0123456789) Scientific Reports | (2024) 14:7122 | https://doi.org/10.1038/s41598-024-56288-8www.nature.com/scientificreports/conditions, 0.73 logit); item 9 (The presence of cracking on the eggshells indication that the hen's eggs are not fit for consume, 0. 62 logits), and item 12 (The present of farms waste on the hen's eggs containers has no effect on hen's egg safety, 0.56 logits).On the other hand, the easiest items to answer were item 7 (The quality of the hen's eggs is influenced in terms of nutritional value by the way chickens are raised (regular or free-range farm) − 2.23 logit); item 13 (There is a minimum boiling time for hen's eggs, to assure the safety before consuming the boiled eggs., -1.68 logit); item 3 (An egg's fluidity increases if it's stored in wrong way,− 0.99 logit); item 2 (The old hen's eggs becomes

Table 2 .
Difficulty measures of knowledge items.

Table 4 .
Participants' knowledge according to their sociodemographic distribution presented in mean ± sd.*Significant, p < 0.05 using Independent sample t-test. 1 one Way Anova, 2 Independent sample t-test.

Table 5 .
Participants' knowledge according to hen's egg purchase practices.*significant p < 0.05 using one way ANOVA test.

Table 6 .
Participants' knowledge according to practice.*significantp < 0.05 using one way ANOVA test.that are dirty may contain harmful bacteria that can enter eggs; and if they are not cooked properly, they can potentially cause food poisoning.Cracked eggs increase the risk of food poisoning