Volumetric changes in temporomandibular joint space following trans-oral vertical ramus osteotomy in patients with mandibular prognathism: a one-year follow-up study

This study measured and analyzed chronological changes in temporomandibular joint space volume by compartment following transoral vertical ramus osteotomy (TOVRO) using reconstructed 3-dimensional (3D) images of patients with mandibular prognathism. It included 70 joints of 35 patients who underwent TOVRO between January 2018 and December 2021. Computed tomography (CT) or cone-beam CT (CBCT) was performed before surgery (T0) and at 3 days (T1), 6 months (T2), and 12 months postoperatively (T3). These scans were then analyzed using 3D software. The volumes of the overall (Vjs), anterior (Vajs), posterior (Vpjs), medial (Vmjs), and lateral (Vljs) joint spaces were calculated at each time point. A linear mixed model and repeated-measures covariance pattern with unstructured covariance were used to evaluate significant changes in joint space volume over time. Vjs significantly increased to 134.54 ± 34.28 mm3 at T3 compared to T0 (p < 0.001). Vpjas and Vljs increased by 130.72 ± 10.07 mm3 and 109.98 ± 7.52 mm3 at T3 compared to T0, respectively (p < 0.001). However, no significant difference was observed between T0 and T2 in Vajs and Vmjs (p = 0.9999). The observed volume increases in Vpjs and Vljs appeared to contribute to the overall Vjs increase.


Changes in Vmjs and Vljs over time (Fig. 1d,e, Table 3)
The medial joint space (Vmjs) showed a significant increase of 149.03 mm 3 (22.53%)between T0 and T1, but decreased thereafter, with no significant difference between T0 and T2 or between T0 and T3.

Discussion
Immediately after TOVRO, sagging of the mandibular condyle occurs because the proximal segment of the joint, including the mandibular condyle, is free from the surrounding tissues except for the lateral pterygoid muscle, joint capsule, and ligaments located in the condylar region.The influence of the lateral pterygoid muscle located in the mandibular condyle induces rotational movement of the proximal segment, directing it in an anteriorinward or anterior-inward-inferior direction, with the contact area of the proximal and distal bone fragments serving as the center of rotation 9,15,16 .
Recovery of proximal segment displacement involves osseous healing, cortical bone remodeling between the proximal and distal segments, and rehabilitation of the surrounding tissues through functional physical therapy 7 .However, a recent study indicates that the downward and rotational movements of the mandibular condyle, occurring immediately after surgery, only partially recover to the preoperative state during the recovery process, and complete recovery is not achieved even after one year 8,9 .This study delves deeper into the joint space, categorizing it into anterior (Vajs), posterior (Vpjs), medial (Vmjs), and lateral (Vljs) compartments, aiming to pinpoint the specific locations where these changes manifest.
Based on our results, the volumes of Vpjs and Vljs remained elevated at T2 compared to T0, while there was no difference between Vajs and Vmjs between T0 and T2.These findings are consistent with the results of Huang et al. 's study, where 6 months post-surgery, the anterior joint space exhibited no significant change while the posterior joint space increased 17 .Nagata et al. 18 also reported on the movement of the condyle according to overall postoperative time changes after IVRO.Similar to this study, it exhibited an immediate increase after surgery that gradually recovered over time.However, unlike this study, Nagata et al. used two-dimensional measurements without segmentation.In addition, Hong et al. 19 reported that the volume of joint space did not recover even six months after surgery.On the other hand, there were studies that reported no change in joint space or a decrease in posterior joint space compared to pre-surgery levels. 20,21These studies targeted patients undergoing SSO, introducing potential variations from our study.
The observed increase in the posterior and lateral joint spaces at T3 is thought to be due to the incomplete recovery of the condyle to its original position.This deviation occurred immediately after surgery in the anteriormedial-inferior direction.The force exerted anteriorly and superiorly, combined with the recovery of the masseter muscle and medial pterygoid function post-surgery 22 , likely influences the direction of recovery.
There was no significant change in the volume of any joint space between 6 and 12 months postoperatively (Tables 1, 2, 3).Predicting the exact timing of the changes in the joint space was challenging because CT images were obtained at 3 days and at 6 and 12 months postoperatively.The authors believe that bone fragment movement after surgery typically occurs within the first 6 months, aligning with previous reports 23,24 .Additionally, tooth movement, which can affect the change in mandibular position during postoperative orthodontic procedures, usually occurs within the initial 6 months due to the regional acceleration phenomenon 25 .Consequently, it may be predicted that alterations in the TMJ space postoperatively will stabilize around the 6-month mark.
Zhao et al. reported that vertical ramus osteotomy has favorable effects on the TMJ in animal studies 26 .Several other studies have also highlighted improvements in TMJ disorders (TMD) following a vertical ramus osteotomy 27,28 .In patients with TMJ disease with concurrent anterior disc displacement, the retrodiscal tissue resides between the glenoid fossa and the mandibular condyle.The displaced retrodiscal tissue, exposed to excessive loads, can trigger an inflammatory response.This inflammatory response in the retrodiscal tissue, rich in nociceptive fibers, significantly influences TMJ pain associated with disc displacement 29 .In this study, both Vjs and Vpjs increased significantly after vertical osteotomy in patients with prognathism.The increased www.nature.com/scientificreports/joint space remained stable even at 6 months postoperatively.Such changes in the joint space can alleviate the deleterious load applied to the retrodiscal tissue, suggesting a possible reduction in TMJ pain.However, since the patients participating in this study did not complain of TMD symptoms, and TMD symptoms themselves were not investigated, a cautious approach is required.There are certain limitations to this study.First, the study did not account for mandibular asymmetry.In 2020, Kim et al. investigated the difference in condylar space change after TOVRO in patients with facial asymmetry and found no significant difference between the deviated and non-deviated sides 8 .However, since patients with mandibular asymmetry may exhibit different effects, a more comprehensive study, considering the presence of mandibular asymmetry, is required.Second, relying on estimated CBCT values on postoperative day 3 could be considered another limitation.However, images were only taken for patients requiring post-operative status assessment and not for all the patients.Third, there were mixed patients who underwent TOVRO alone (17.1%).This study was conducted to investigate changes in temporomandibular joint space after IVRO.Recent studies have combined patients who underwent LeFort I osteotomies with those who did not [30][31][32] .However, since this may affect the results, future research is needed to distinguish between these patient groups.Moreover, employing computer-aided measurement techniques 33 , or artificial intelligence technology 34 , which has been widely studied recently, can help improve human subjectivity in the future.In this study, CT and CBCT were used for pre-and post-operative analyses.Pellerin et al. 35 reported that the volume of liver lesions measured by CBCT and CT in rabbit experiments was almost the same size [3.8 ± 1.6 cm 3 (range, 1.3-7.3cm 3 ) on CBCT and 3.9 ± 1.6 cm 3 (range, 1.8-7.5 cm 3 ) on MDCT].Trindade et al. 36 also reported that there was no difference in the volume of periapical lesions measured by CBCT and CT.Additionally, the Mimics program used in this study is reported to be quite accurate compared to other software 37 .Nevertheless, a strength of this study lies in the detailed analysis of volumetric changes in the TMJ space, divided into compartments, in patients who underwent TOVRO.The volume of the TMJ space at 1-year follow-up was higher than that before surgery, which is thought to be due to the increase in the posterior and lateral joint spaces.In addition, the joint space appeared to have stabilized six months after surgery.

Materials and methods
This retrospective study involved the medical records of patients who were diagnosed with mandibular prognathism and underwent orthognathic surgery between January 2018 and December 2021 at the Department of Oral and Maxillofacial Surgery, Gangnam Severance Hospital, Seoul, Republic of Korea.The exclusion criteria for this study included the following: (1) patients without CBCT images after surgery; (2) poor or inappropriate image quality; (3) patients with pathological conditions such as degenerative joint disease, condylar osteochondroma, or synovial chondromatosis; and (4) patients with congenital facial anomalies such as cleft lip and palate and hemifacial microsomia.
A total of 35 patients were included in the study.The sample size was calculated using the G-power program based on a previous study 8 (Power = 90%, α = 0.05).Patients underwent preoperative (T0) CT scans for analysis, diagnosis, and simulation surgery, with the fabrication of a rapid prototype model two months before the operation.All patients underwent CBCT at 6 (T2) and 12 months (T3) post-surgery for postoperative follow-up.Of them, 19 patients underwent additional follow-up CBCT on day 3 (T1) post-surgery to assess the surgical results.
Postoperative management adhered to a predefined protocol 7 .During the two weeks following surgery, intermaxillary fixation was performed using surgical archwires and six elastics.Subsequently, physical therapy, including mouth opening and lateral movement, was carried out using two elastics until maximum mouth opening was restored.The splint was then removed, and postoperative orthodontic treatment was initiated.
This study was approved by the Institutional Review Board of Gangnam Severance Hospital (approval No. 3-2022-0466), and a waiver of written informed consent was granted for this retrospective study.This study was conducted in accordance with the principles of the Declaration of Helsinki for human research. 38ndibular prognathism was diagnosed using lateral cephalometric radiography.The Frankfort horizontal line (the line connecting the porion and orbital; the FH line) was set as the horizontal reference line.The vertical reference line was set as the line perpendicular to the horizontal FH line passing through the nasion point (N-perpendicular line).Mandibular prognathism was diagnosed when the pogonion was more than 1 mm anterior to the vertical reference line.

Reconstruction and CT image analysis
CT images were obtained using a Siemens Definition AS + (Siemens, Erlangen, Germany) with constant settings (1 mm slice thickness, 7 s scan time, 120 kV, and 90 mAs).CBCT images were obtained using a PaX-i3D (Vatech Co., Gyeonggi-do, Republic of Korea) with constant settings (21 cm x 19 cm field of view, 0.3 mm slice thickness, 24 s scan time, 106 kV, and 65 mAs).The images were reconstructed and analyzed using Mimics 3D analysis software (Materialise, Leuven, Belgium).This process was done by one oral and maxillofacial specialist (H.S. Yong, 6-year experience).
Based on previous studies, the mid-axial plane (MAP), mid-sagittal plane (MSP), and coronal plane (COP) were established 7 .The MAP was defined as the plane connecting the OrR, OrL, and PoR.The MSP was defined as a plane perpendicular to the MAP passing through the Cg and Ba.The COP was defined as the plane passing through Ba and perpendicular to the MAP and MSP.The plane parallel to the MAP passing through the lateral lip of eminence point (Em) was defined as the eminence plane (EmP).The planes parallel to the MSP and COP and passing through the Gf were defined as GfSP and GfCP, respectively (Fig. 2).The results are summarized in Table 4.

Superimposition of reconstructed 3D images
The preoperative CT images and 3-day, 6-month, and 12-month postoperative CBCT images were superimposed onto the cranial region.
The first overlap was performed using the 3-point superimposition method with three reference points (Na, PoR, and Ba) of the skull region, where there was no change in position or shape before and after surgery in the sagittal, coronal, and horizontal cross-sections Additionally, minor discrepancies were rectified so as to make the cranial region superimposition more accurate.An illustrative example of this superimposition is provided in Fig. 3.

Measurement of volume of joint space on T0
In a previous study 11 , the volume of the glenoid fossa (Vgf) was measured first, and the volume of the joint space (Vjs) was measured by removing the condyle.For this study, all the images were captured by H.S. Yong.Vgf and Vjs were calculated as follows: (1) First, the mandible was separated from the 3D-reconstructed skull (Fig. 4a,b).
(2) A curved plane connecting the lowest rim of the articular eminence and zygomatic arch was formed at the anterior and posterior boundaries, respectively (Fig. 4c,d).
(3) The temporal bone, petrotympanic fissure, and sphenoid bone borders were set as the superior, posterior, and medial borders, respectively.(4) The space bounded above was separated by the eminence plane (EmP), and the volume of the superior space was measured as the volume of the glenoid fossa (Vgf) (Fig. 4e,f).(5) After removing the mandibular condyle from the glenoid fossa formed above, the remaining space was measured as the TMJ space volume (Vjs) (Fig. 5a,b).( 6) The TMJ space (Vjs) was subdivided into the glenoid fossa coronal plane (GfCP), and the anterior and posterior parts were measured as the volume of the anterior joint space (Vajs) and posterior joint space (Vpjs) (Fig. 5c).( 7) Similarly, the TMJ space (Vjs) was subdivided into the glenoid fossa sagittal plane (GfSP), and the medial and lateral areas were measured as the volume of the medial (Vmjs) and lateral joint space (Vljs), respectively (Fig. 5d).

Figure 1 .
Figure 1.Volumetric changes of joint spaces over time.(a)Vjs, the volume of the joint space; (b) Vajs, the anterior joint space; (c) Vpjs, the posterior joint space; (d) Vmjs, the mesial joint space; (e) Vljs, the lateral joint space.

Figure 2 .
Figure 2. Reference planes for 3D analysis.(a) Right side of the skull; (b) Left side of the skull EmPR and EmPL are planes parallel to MAP, passing through EmR and EmL.(c) occlusal view of the mandible GfSP and GfCP are planes passing through Gf and parallel to MSP and COP, respectively (GfSPR and GfCPR indicate right side, and GfSPL and GfCPL indicate left side).Reconstructed joint space is presented in yellow.MAP, Mid-axial plane; MSP, Mid-sagittal plane; COP, Coronal plane; EmPR, Eminence plane right; EmPL, Eminence plane left; Gf, Glenoid fossa (the most superior point of Glenoid fossa); GfSPR, Glenoid fossa sagittal plane right; GfCPR, Glenoid fossa coronal plane right; GfSPL, Glenoid fossa sagittal plane left; GfCPL, Glenoid fossa coronal plane left.

Figure 3 .
Figure 3. Example of Superimposition of 3D Image.(a) Pre-operative image; (b) Superimposition of skull between pre-operative and post-operative 6 M images.(c) Superimposition of skull among pre-operative, postoperative 3 days, and post-operative 6 M images.(d) Superimposition of mandible between pre-operative and post-operative 6 M images (Yellow, skull before surgery; blue, mandible before surgery; purple, post-operative 3 days; red, post-operative 6 months).

Figure 5 .
Figure 5. Measurement of volume of the joint space (Vjs).(a, b) The volume of the joint space (Vjs).The Vjs was measured after removing of reconstructed mandible from Vgf; (c) the anterior and posterior joint space (Vajs and Vpjs).Vjs was divided by GfCP for Vajs and Vpjs; (d) The mesial and lateral joint space (Vmjs and Vljs).Vjs was divided by GfSP for Vmjs and Vljs, Vgf, Volume of glenoid fossa; GfSPR, Glenoid fossa sagittal plane right; GfCPR, Glenoid fossa coronal plane right.

Table 1 .
Changes of overall temporomandibular joint space volume (Vjs) over time.Vjs, the volume of the joint space.

Table 2 .
Changes of anterior and posterior temporomandibular joint space volume (Vajs and Vpjs) over time.Vajs, the anterior joint space; Vpjs, the posterior joint space.

Table 3 .
Changes of medial and lateral temporomandibular joint space volume (Vmjs and Vljs) over time.Vmjs, the mesial joint space; Vljs, the lateral joint space.

Table 4 .
Reference points and planes for 3D analysis.