New Au/chitosan nanocomposite modified carbon paste sensor for voltammetric detection of nicotine

A profoundly touchy voltammetric sensor for detection of nicotine (NIC) in urine and tobacco specimens has been developed in light of the boosted electrochemical response of NIC at gold and chitosan nanocomposite modified carbon paste electrode (ACMCPE). Material characterization techniques Scanning Electron Microscope and Energy Dispersive X-ray (SEM & EDX) were utilized to describe the ACMCPE surface material. The impedance spectroscopy technique (EIS), cyclic voltammetry (CV), chronoamperometry (CA), and differential pulse voltammetry (DPV) were employed to explore the electrochemical sensing of NIC at ACMCPE. The created sensor exhibits an exceptional electrochemical sensitivity to NIC in a universal Britton–Robinson (B-R) buffer solution with a pH range of 2.0 to 8.0. The sensor shows a linear response over NIC concentration ranges of 4.0–320.0 µM, with the detection limit (LOD) of 7.6 µM. The prepared sensor has been shown to be exceptionally viable in detecting NIC with amazing selectivity and reproducibility. We suggest it as a trustworthy and useful electrochemical sensor for NIC location.


Reagents and solutions
The standard NIC analyte sample (99%) was supplied by the Egyptian Smoking Eastern Company and was used without prior refinement.The freshly made 1.62 g L −1 NIC stock solutions in water were then stored in a dark container due to the compound's light sensitivity.Paraffin oil mixed with graphite fine particles, supplied by Merck, were used to set up the CPE.Gold chloride salt (KAuCl 4 ) and chitosan powder were provided by Aldrich (USA).(B-R) buffer solutions (4.0 × 10 -2 M), utilized as supporting electrolytes, were prepared from CH 3 COOH, H 3 BO 3 and H 3 PO 4 at pH values ranging from 2.0-8.0.PH values were changed utilizing HCl (0.2 M) and NaOH (0.2 M).Each experiment produced repeatable findings after being run two to three times at laboratory room temperature.

Modified electrodes construction
A homogenous paste was made by mixing 2 mL of paraffin oil with 5.0 g of graphite powder in a mortar for 5 min to prepare the bare carbon paste electrode (denoted as BCPE) 33,34 .In order to find the optimal sensor composition, several attempts were made to create the modified CPE with chitosan by mixing various amounts of chitosan into the paste and assessing their response toward NIC.On that principle, 0.4 g of CP and 0.1 g of chitosan powder were prepared to create a Chitosan/CPE (denoted as CCPE).Every composite combination was then stuffed into the finish of a Teflon tube (a hole of 3 mm in diameter which equals 0.0706 cm 2 electrode surface area) and polished with ultrafine emery paper to obtain a smooth surface.In order to prepare the Au-NPs/Chitosan/CP electrode (denoted as ACMCPE), the CMCPE was submerged in a solution of gold chloride (0.01 M) for 300 s to deposit Au-NPs on the surface of the CMCPE at a deposition potential of -0.4 V.It was then dried for 5 min by air.

Cell and devices
The electrochemical investigations were carried out at room temperature using a three-electrode cell with a capacity of 25 mL, consisting of an ACMCPE as the working electrode, a platinum auxiliary electrode (CE), and a calomel reference electrode (RE).Electrochemistry techniques such as DPV, CV, CA and EIS were carried out by SP-150 potentiostat connected to the EC-Lab® software package.EIS measurements were carried out at 10 mV ac amplitude in the frequency range of 1.0 mHz to 100 kHz.EC-Lab ® programming was utilized to fabricate the best comparable circuit models [35][36][37] .pH measurements were performed using a pH-meter from Hanna Instruments in Italy.Energy Dispersive X-ray analyzer (Bruker) and SEM (TESCAN VEGA3, Czech Republic) were used to evaluate the electrode's surface morphology.Transmission electron microscopy (TEM) analysis was made using a JEM-1400 Electron Microscope (JEOL, Japan).

Analysis of urine
Real urine samples were examined using a diluted pee solution created by diluting 400 times in 100 mL of B-R buffer pH 2 in order to create a stock solution.A solution containing NIC in B-R buffer at pH = 2 was used to establish standard increments of NIC at different concentrations to get the calibration graph.

Cigarette sample examination
Cigarettes were dried for 30 min on a 40 °C stove after being taken out of their rolling papers.10 mL of water and 0.1 g of tobacco from a combination of 10 cigarettes (from two packs of a comparable brand) were added, and the mixture was then sonicated for 3 h in an ultrasonic water bath and filtered.The B-R buffer (pH 2.0) was mixed with the appropriate volume (100 mL) of the filtrate and evaluated under the same conditions as those used to obtain the calibration graph 5 .

Morphology of the investigated electrodes
SEM micrographs were used to examine the surface morphology of the Au-NPs that had been deposited on Chitosan/CPE.The Au-NPs have an approximately spherical shape and are dispersed randomly at varying altitudes over the electrode surface, as indicated by the SEM images of the ACMCPE surface at several amplifications shown in Fig. 1a-c.This suggests a significant surface area.According to the scale of the image, the size of Au-NPs varies between 30 and 50 nm as depicted in Fig. 1c.Only 85 nm particle was odd one and not repeatable across the whole prepared surface, which enhanced our surface stability and its well preparation procedure.Also TEM images enhanced our findings and the sizes were nearly from 15-30 nm as reported in Fig. 1e.The different particle sizes observed might be explained by nucleation, agglomeration, and subsequent growth.The EDX analysis of the ACMCPE sensor is shown in Fig. 1d, which verifies the presence of C, O, and Au by a percentage of 84.57%, 1.09%, and 14.34% respectively, confirming that Au-NPs were indeed coated on the Chitosan /CPE surface.

Electrochemical measurements
The CV and EIS were used to evaluate the electrochemical properties of NIC at CPE, CCPE, and ACMCPE.Figure 2A shows typical voltammograms of 1.0 mM NIC in a B-R buffer (pH 2) produced by scanning the three working electrodes at a rate of 50 mV s −1 .The oxidation of NIC at ACMCPE results in an irreversible oxidation peak (voltammogram ACMCPE) at + 1050 mV and Ip = 82.35μA.In contrast, no redox response was observed for BCPE and a small Ip = 8.9 µA at a potential of around + 1000 mV was observed for CCPE, suggesting that the electrodeposition of Au-NPs on to the surface of the chitosan/CPE was a successful technique for improving NIC detection.
The Randles-Sevcik equation [38][39][40] was utilized to determine the active surface area of the modified electrode for a known K 4 [Fe(CN) 6 ] concentration: where Ip is the peak current (A), ν is the scan rate (V s −1 ), A is the electrode area, n is the number of transferred electrons, D the diffusion coefficient which equals to 7.6 × 10 -6 cm 2 s −1 , and C is the concentration of K 4 [Fe(CN) 6 ].For 1.0 mmol L −1 K 4 [Fe(CN) 6 ] in 0.10 mol L −1 KCl electrolyte with n = 1.
The bare electrode area was calculated using the hole diameter of 3 mm using πr 2 = 0.0706 cm 2 and after deposition of Au-NPs, the active surface area of ACMCPE was increased to 0.144 cm 2 .
Nyquist plots were exhibited at + 1050 mV (E p from CV) for ACMCPE, CCPE, and BCPE (Fig. 2B).In the high-frequency region, the semicircle diameter of EIS corresponds to the charge transfer resistance (R CT ) at the electrode electrode/electrolyte interface.A comparison of the semicircular regions of the three EIS curves at high frequency shows that the modified electrode ACMCPE has a lower R CT than the bare electrode and CCPE [41][42][43] .The Randles circuit model was used to fit the acquired EIS curve (Fig. 2B inset) including R CT , solution resistance (R s ), constant phase element of capacitance (CPE) and Warburg impedance (Z w ) [44][45][46] .The fitting was done with EC-Lab ® software supplied with the SP-150 workstation.The ACMCPE electrode was shown to have higher values for Z w (1380 Ω cm 2 s −1/2 ), CPE (18.7 µF cm −2 ), and a lower value for the R CT (2243 Ω cm 2 ) demonstrating greater conductivity than bare CPE and CCPE with CPE = 6.3 µF cm −2 , W = 456 Ω cm 2 s 1/2 and R CT = 12,960 Ω cm 2 and CPE = 8.6 µF cm −2 , W = 488 Ω cm 2 s −1/2 and R CT = 11,250 Ω cm 2 respectively.Accordingly, the considerable decrease in R CT for ACMCPE compared to BCPE and CCPE can be attributed to Au-NPs deposited on CMCPE, which forms many electrocatalytic centers, lowering the contact resistance between ACMCPE/electrolyte interfaces.These findings back up the high anodic current I p value obtained from CVs for ACMCPE electrodes.

Influence of pH
The cyclic voltammogram approach was used to assess the impact of pH on NIC oxidation peak utilizing B-R buffer (pH 2-8) at both the BCPE and ACMCPE.According to Fig. 3, the anodic Ep moved negatively with rising pH values, demonstrating that protons are involved in electrode reactions and that the electrocatalytic oxidation of NIC was a pH-dependent process [47][48][49] .In addition, the linear relationship between Ep for NIC oxidation and pH (Ep = − 0.06 pH + 1.16 (R 2 = 0.99) was demonstrated (see Fig. 3 inset B).According to the slope value, the electrode reactions demand an equal number of electrons and protons.At acidic and basic media, it is also obvious that peak potential varies with pH.At pH 2, the maximum peak current signal was obtained, which gradually decreased from 2.0 to 6.0 before increasing at pH 8. Accordingly, depending on the pH of the media, NIC has two pKa values: 3.12 and 8.02, which are equivalent to the monoprotonated (protonation of pyrrolidine nitrogen) and diprotonated (pyridine nitrogen) forms of the NIC molecule, respectively 50,51 .These results could indicate that NIC oxidation took place via protonation of nitrogen atoms on the pyrrolidine ring and was assigned to tertiary nitrogen oxidation.
Figure 4 shows Nyquist plots of NIC at different pH values utilizing ACMCPE at E p vs. SCE.The EIS results are consistent with those obtained from CV.At different pH solutions, the Nyquist plot of ACMCPE shows almost straight lines at lower frequencies and tiny incomplete semicircles at higher frequencies, which correspond to (1) www.nature.com/scientificreports/diffusion-limited and electron transfer-limited processes [52][53][54] .The Nyquist plot at pH 2 shows a substantially smaller semicircle in the high-frequency region than other pH solutions, implying that ACMCPE at pH 2 has greater electron transport capabilities.

The influence of scan rate
In order to investigate the kind of the electrochemical process on the ACMCPE electrode, the impact of the scan rate (v) on the electrooxidation of NIC (1 × 10 -3 M) was studied at various scan rates ranging from 10 to 200 mV s -1 using CV in B-R buffer (pH 2).It is clear that, in Fig. 5, as the scan rate increased, the peak potential (Ep) of the oxidation of NIC went considerably to higher positive values, demonstrating the irreversibility of the reaction 55,56 .A plot of the anodic peak currents (Ip) and the square root of scan rate (v 1/2 ) revealed good linearity (Ip = 15.2 × ν 1/2 + 20.6) with a correlation coefficient of r 2 = 0.988 (see Fig. 4 inset), indicating that the charge transfer was a diffusion-controlled process 57,58 .In addition, the diffusion coefficients, D app, of NIC in B-R buffer (pH 2.0) utilizing ACMCPE and CMCPE were derived from the relationship between the squire root of the scan rate v 1/2 and the anodic peak current I pc (A) [Fig.5 inset] based on the Randles-Sevcik equation [59][60][61] .
In this equation, n is the number of electrons exchanged in oxidation at T = 298 K, C 0 is the analyte concentration (1 × 10 −6 mol cm −3 ), D is the electroactive species diffusion coefficient (cm 2 s −1 ), F is the Faraday constant in C mol −1 , ν is the scan rate in V s −1 , A is the geometrical electrode area (cm 2 ), and R is the gas constant in VC (2) I p = 0.4463 nFAC 0 (nFvD/RT) www.nature.com/scientificreports/K −1 mol −1 .Thus, the apparent diffusion coefficients, D app, of NIC at ACMCPE and CMCPE were found to be 8.841 × 10 −5 cm 2 s −1 and 5.12 × 10 −5 cm 2 s −1 , respectively.

Chronoamperometry
The chronoamperometric response of NIC in B-R buffer (pH 2.0) was recorded at a constant potential (+ 1050 mV vs. SCE) utilizing ACMCPE sensor.The NIC calibration graph, shown in Fig. 6 inset B, was created by plotting NIC concentrations versus oxidation currents at a given time (1 s).A linear relationship was observed from 20 to 600 μM with a linear equation of Ip = 0.062 C + 15.74, and a correlation coefficient of 0.96.Based on the Cottrell equation [62][63][64] , the diffusion coefficient can be calculated from the equation.where i = current, in unit A, F = Faraday constant (96,485 C/mol), n = number of electrons, c 0 = bulk concentration of the analyte in mol/cm 3 ; A = area of the electrode in cm 2 , D = diffusion coefficient for species in cm 2 /s and t = time in s.The slope obtained from the relationship between the anodic peak current and t -1/2 (Fig. 6 inset A) equals nFAc o (D/π) 1/2 , in accordance with the Cottrell equation, so the diffusion coefficient of NIC was calculated to be 7.38 × 10 -5 cm 2 s -1 .The NIC oxidation reaction is shown to be controlled by a diffusion control process, since the diffusion coefficient values estimated from chronoamperometric measurements (CA) are equivalent to those calculated from CV measurements.

Calibration plot
The DPV response of ACMCPE electrode to different NIC concentrations in B-R Buffer (pH 2) was measured at 10 mV s −1 , (Fig. 7).A distinct response was noted with the sequential addition of NIC, as indicated in Fig. 7.These results reveal that Au-NPs coated on a chitosan/CPE surface have a stable and efficient catalytic capability.Under optimal conditions, the response currents and NIC concentrations have a linear relationship in the range 4 × 10 -6 -3.2 × 10 -4 M. The plot of anodic peak currents vs. NIC concentrations (see Fig. 7 inset) demonstrated a linear regression with the following equation: Ipa (A) = 0.065 C + 22.76 and r 2 = 0.96.The following equations have been used to calculate the limits of detection (LOD) and quantification (LOQ): where m is the slope (μA/M) of the calibration curves and s is the standard deviation (SD) (three runs); they were found to be 7.6 × 10 −6 M and 2.3 × 10 −6 M, respectively 65,66 .
Comparisons of data acquired for determination of NIC using different instruments and electrochemical approaches versus ACMCPE were recorded in Table 1.Although it has roughly the same specific selectivity, the sensor has the benefit of employing non-poisonous and less expensive reagents.The ACMCPE sensor is less sophisticated and expensive than spectrometry equipment, HPLC, GC, and flow injection.Additionally, the   pre-treatment and processes for these technologies are challenging, in contrast to how easy and uncomplicated they are for the ACMCPE sensor.

Interference study, reproducibility and stability
The interference effect on the voltammetric response for NIC was used to inspect the selectivity of the constructed sensor.It was tested in the presence of caffeine, ascorbic acid, uric acid, sucrose, lactose, and glucose, which are common in human urine and can interfere with NIC.The voltammetric response of various interferants was measured at anodic peak of Ep = + 1050 mV, with essentially negligible current response.Due to the low concentration of other minor alkaloids (0.2-0.5 percent of total alkaloids), which cannot affect the precision of NIC detection at the sensitivity level of voltammetric tests, the interference of alkaloids that may be present in tobacco was not addressed in this work.Because cotinine and NIC are structurally related, using them together would completely define the selectivity of the proposed sensor.A constant concentration of NIC (500 μM) was spiked with similar and two-fold concentrations of the NIC biomarker "cotinine".The same experimental settings were applied for CV measurements, and recovery data ranging from 99.3% to 100% were obtained, suggesting that the modified ACMCPE might be a useful electrocatalytic sensor for detecting NIC while avoiding interference.The sensor's reproducibility was assessed by contrasting the current responses of five independent constructed ACMCPEs for 100 µM NIC.It gives a similar result, with a variation of only 4.6%.This confirms that the preparation process of the electrode is highly reliable and consistent.The long-term stability of one electrode was also tested by determining its current response every three days; the electrode was kept in the air.After two weeks, the response is 95% of its initial value, and then drops to 90% after three weeks, indicating a relatively consistent performance for NIC detection.

Usage of the ACMCPE sensor in urine
In order to determine the methodology's applicability, the ACMCPE sensor was utilized to count the concentrations of NIC in urine samples.The calibration plot, shown in Fig. 8, produced a straight line that obeyed the linear relationship with r 2 = 0.968, I pa (µA) = 0.074 C + 17.05, and a LOD of 2.07 µM.On the calibration plot, four separate concentrations are chosen, and each is repeated five intervals to reveal the procedure's accuracy and precision.The concentration recovery was judged to be between 96.0-100.6%as shown in Table 2.

Examination of real cigarettes samples
To guarantee that the suggested procedure was validated with actual samples, two products from different cigarette products (Marlboro and L&M) were examined for a real application.This study employed a slightly amended version of the procedure supplied by Suffredini et al. 49 .To acquire the NIC value of the tobacco sample, an appropriate amount of standard NIC solution generated in the supporting electrolyte was added to the previously  www.nature.com/scientificreports/formed NIC content.The recommended method's ability to quantitatively recover NIC quantities demonstrates the accuracy of the NIC detection in tobacco.The recovery data supports this assertion.(See Table 3).

Conclusions
In this study, we constructed a novel NIC sensor by electrodepositing Au-NPs onto a chitosan/CPE surface.This sensor showed outstanding NIC sensing performance due to the increase in the electro-active area developed by Au-NPs as revealed by the CV and EIS experiments.In the electrochemical NIC response, it showed a wide linear detection range (4 × 10 -6 -3.2 × 10 -4 M) with a limit of detection 7.6 × 10 -6 M. The sensor has a good selectivity, sensitivity, and stability for NIC determination.Interference of any accompanying substrates wasn't detected.
Additionally, it has been demonstrated that the sensor can accurately identify NIC in both urine and cigarette samples.As suggestion for future, a transfer from the success of NIC detection to cotinine detection based on ACCMCPE will broaden the analytical applications as Cotinine is the metabolite of NIC and structurally similar to it.Also screen printed electrode or any other graphite derivatives as carbon nanotubes, graphene or graphene oxide for NIC detection may be applied in future investigations.

Figure 7 .
Figure 7. Effect of NIC addition in B-R buffer (pH 2.0) sequentially utilizing ACMCPE at 10 mV s −1 .Inset: NIC calibration curve utilizing ACMCPE.The standard deviations of three repeated tests are shown as error bars.

Table 1 .
Contrasting the recommended method with other techniques and electrodes for NIC measurement.

Table 2 .
The ACMCPE sensor's accuracy and precision for NIC detection in a urine sample.a Mean for five determinations.

Table 3 .
NIC recovery analysis in cigarette tobacco.