Quantitative [68Ga]Ga-PSMA-11 PET biomarkers for the analysis of lesion-level progression in biochemically recurrent prostate cancer: a multicentre study

[68Ga]Ga-PSMA-11 PET has become the standard imaging modality for biochemically recurrent (BCR) prostate cancer (PCa). However, its prognostic value in assessing response at this stage remains uncertain. The study aimed to assess the prognostic significance of radiographic patient-level patterns of progression derived from lesion-level biomarker quantitation in metastatic disease sites. A total of 138 BCR PCa patients with both baseline and follow-up [68Ga]Ga-PSMA-11 PET scans were included in this analysis. Tumour response was quantified at the lesion level using commonly used quantitative parameters (SUVmean, SUVmax, SUVpeak, volume), and patients were classified as systemic, mixed, or no-progression based on these response classifications. A total of 328 matched lesions between baseline and follow-up scans were analysed. The results showed that systemic progressors had a significantly higher risk of death than patients with no progression with SUVmean demonstrating the highest prognostic value (HR = 5.70, 95% CI = 2.63–12.37, p < 0.001, C-Index = 0.69). Moreover, progressive disease as measured by SUVmean using the radiographic PSMA PET Progression Criteria (rPPP) was found to be significantly prognostic for patient overall survival (HR = 3.67, 95% CI = 1.82–7.39, p < 0.001, C-Index = 0.65). This work provides important evidence supporting the prognostic utility of PSMA response quantitation in the BCR setting.


Patient cohort
This retrospective study utilised a cohort of 238 patients with BCR PCa who were imaged as part of a prospective trial registered with the Australian and New Zealand Clinical Trials Registry (ACTRN12615000608561) 8 .Patients were imaged at one of two hospitals in Perth, Western Australia-Sir Charles Gairdner Hospital (SCGH) or Fiona Stanley Hospital (FSH).To be included in this prospective trial, patients had to meet the following criteria: (1) present with biochemically recurrent disease after definitive primary therapy, based on serum prostate-specific antigen (PSA) values, defined as either a PSA level > 0.2 ng/mL at > 6 weeks post radical prostatectomy or a PSA level 2 ng/mL above the previous nadir measurement at 3 months post external beam radiotherapy, and; (2)  have either oligometastatic (≤ 3 lesions) or negative disease on conventional staging imaging (abdominopelvic contrast CT and bone scintigraphy).Out of the 238 patients, 199 received both baseline and follow-up [ 68 Ga] Ga-PSMA-11 PET/CT scans approximately 6 months later, and therefore, 39 patients were excluded from the analysis.Between the baseline and follow-up scans, patients were treated according to the standard of care as determined by the treating physician, including: active surveillance, radiotherapy to either the prostatic bed or metastatic disease sites, additional surgical procedures, systemic hormonal treatment, or chemotherapy.All methods carried out in this study were conducted in accordance with the ethical standards of the SCGH Human Research Ethics Committee, and appropriate ethics approval was obtained (RGS1736).The research was conducted in line with the principles of the Declaration of Helsinki.An overview of the patient inclusion criteria and study design is provided in Fig. 1.

Scan acquisition
[ 68 Ga]Ga-PSMA-11 PET/CT scans were acquired 60 min after the intravenous injection of 2 MBq/Kg of [ 68 Ga] Ga-PSMA-11 on either a Siemens Biograph 64 or a Siemens Biograph 128 PET/CT scanner (CTI Inc, Knoxville TN).Patients were asked to void their bladders just prior to commencement of imaging.PET data was acquired immediately after a low dose CT acquisition (50 mAs, 120 kvP) for attenuation correction, with identical fields of view.PET images were reconstructed to an axial plane pixel size of 4.07 × 4.07 mm 2 , while CT images were reconstructed to a pixel size of either 0.98 × 0.98 mm 2 or 1.52 × 1.52 mm 2 .Additional scanning protocol information is provided in Supplementary Table 1.

Manual lesion delineation
An expert nuclear medicine physician (J.O.) retrospectively analysed and segmented PCa lesions in the patient scans.Interpretation of scans was done in accordance with published E-PSMA 5-point scoring guidelines 26 .Areas of elevated tracer uptake were determined to be PCa lesions if they were interpreted as either 'definitely' or 'probably' positive according to these guidelines.Disease sites were segmented with a semi-automated approach, beginning with a threshold of 3 SUV normalised to the patient body weight (SUV bw ), applied to the PET image.The segmentation mask generated by this threshold was then manually altered, removing any physiological uptake that was mistakenly included, and adding any pathologic uptake areas that were missed.All segmentations were performed on the MIM Encore software (MIM Software Inc., Cleveland, OH, USA).Lesions were manually matched between baseline and follow-up imaging to enable the quantification of imaging biomarkers

Lesion-level biomarker quantification
Lesion-level response was assessed using a variety of different imaging biomarkers quantified for each lesion at both baseline and follow-up imaging.The imaging biomarkers included: SUV max , SUV mean , SUV peak , and volume.Given the non-standardised definition of SUV peak , a multitude of different SUV peak definitions were tested.Six   www.nature.com/scientificreports/different spherical radii were used (ranging from 2.5 to 8.75 mm in increments of 1.25 mm) for two different types of SUV peak definitions (centered on the lesion voxel with the maximum uptake, or iterated over all lesion voxels to determine highest uptake region), yielding 12 different definitions of SUV peak that were investigated (see Supplementary Table 2 for a further description of the biomarkers).The percentage change in biomarkers for each lesion between baseline and follow-up was quantified and used to classify lesions into three response categories-partial response, stable, and progressing.Lesion-level response classifications were determined using three different response assessment thresholds: ± 20%, ± 30%, and ± 40% change in each considered biomarker.

Patient-level patterns of progression
Using the lesion-level classifications for each biomarker at each different response assessment threshold, patientlevel patterns of progression were determined and analysed.These patterns were predefined prior to the conduction of the analysis according to previously published patterns of progression for patients with metastatic disease 21 , in which patients were classified as having: (1) systemic progression, (2) mixed progression, or (3) no progression.These patterns are defined in Table 1.
A special case of the patient-level patterns described above should be noted.Considering a ± 30% threshold for lesion progression, and combining the two categories of "systemic" and "mixed" progression into a single progression category, "progressive disease" (PD), the criteria elucidated above become almost equivalent to the PPP criteria 14 .The exception is that as per the aims of this study, progression is assessed purely radiographically, without the requirement of confirmatory clinical or laboratory data.We may therefore define the radiographic PPP (rPPP) interpretation, where patient-level PD results from one of the following: (1) Increase in the size or uptake of any lesion by ≥ 30%, or (2) Appearance of 1 or more new PSMA-positive lesions.This definition is consistent with the PSMA PET/CT response assessment consensus statement 27 , where the appearance of even one new lesion in early recurrent disease is to be considered PD.

Statistical analysis
Comparisons of continuous variables (baseline PSA values and tumour burden) between progression groups were conducted using the Mann-Whitney U-test.Two-way comparisons of the quantified biomarker distributions between baseline and follow-up were performed using the Wilcoxon-signed rank test.Both tests were performed using the SciPy Python package version 1.7.3.The association between patient-level patterns of progression categories and patient OS (measured from the date of classification of progression pattern at follow-up imaging until the date of death) was assessed using the Kaplan-Meier method with the log rank test and univariate Cox regression analysis.Hazard ratios (HRs) were derived, and Harrell's concordance index (C-index) was calculated.Survival analysis was performed with the Lifelines package version 0.27.1.In the case of deriving HRs for all candidate biomarkers at the three thresholds investigated, the Bonferroni correction was applied to correct for multiple testing by multiplying calculated p values by the number of tests conducted.In all cases, a p-value of less than 0.05 was considered to be a statistically significant difference.All statistical analysis were conducted in Python version 3.9.

Ethics approval
Ethics approval for undertaking this study was acquired from the Sir Charles Gairdner Hospital Human Research Ethics Committee (RGS1736).

Consent to participate
Informed consent was obtained from all individual participants included in the study.

Patient and lesion characteristics
In total, 199 patients with both baseline and follow-up PSMA imaging were included in this study.42 of these patients (21.1%) had no measurable disease at baseline imaging, resulting in no lesions to match with the followup scan.A further 19 patients were excluded for having follow-up imaging greater than 1 month away from the specified 6-month time point due to the potential for this to affect the analysis conducted.From the remaining 138 patients, 536 lesions were identified at baseline imaging, with a median number of 2 lesions identified per patient (range: 1-34).The demographic and clinical characteristics of these patients are presented in Table 2.The majority of patients (n = 75, 54.3%) included in the analysis underwent Androgen Deprivation Therapy (ADT) between scans, 43 (31.2%)underwent radiotherapy, and 5 (3.6%) were administered chemotherapy treatments.
Table 1.Lesion-level patterns of progression defined.Progressing lesions includes lesions that are progressing between baseline and follow-up, as well as new lesions identified at follow-up imaging 21 .PD, Progressive disease.www.nature.com/scientificreports/Additionally, 39 patients (28.3%) did not receive any treatment between scans and underwent disease surveillance instead.Among the baseline lesions, 208 were not identified at follow-up imaging and were therefore classified as completely responding, leaving a total of 328 matched lesions for which the percentage differences in baseline and follow-up biomarkers could be quantified.Summary statistics of all biomarkers extracted from these lesions at both baseline and follow-up are presented in Table 3, and boxplots of the percentage response according to each biomarker are presented in Fig. 2. A total of 136 new lesions were identified between baseline and follow-up scanning, with 28 patients (20.3%) presenting new disease sites.This was heavily skewed by one particular patient who presented with 46 new disease sites at follow-up imaging.To enable survival analysis,

Classification discordance
Overall, there was substantial heterogeneity in lesion-level classifications depending on the choice of imaging biomarker and response assessment threshold.When the response assessment threshold was held constant, a considerable number of lesions were classified differently across the range of imaging biomarkers.Specifically, for the ± 20%, ± 30%, and ± 40% response assessment thresholds, 206 (62.8%), 201 (61.3%), and 205 (62.5%) lesions, respectively, had discordant classifications (refer to Table 4).An example of a lesion classified as all response categories is provided in Supplementary Fig. 1.The full lesion classifications for all biomarkers and response assessment thresholds are provided in Supplementary Table 3. Subgroup analysis focusing on specific imaging biomarkers revealed less heterogeneity among the various SUV peak measurements (17.7%, 12.8% and 12.2% of lesions for the ±20%, ±30% and ±40% response assessment thresholds, respectively, Supplementary Table 4) and, excluding only volume, the SUV biomarkers (26.2%, 22.9%, and 23.2%, respectively; refer to Supplementary Table 5), indicating that volume contributes substantially to the observed classification heterogeneity.Out of the 138 patients with identified lesions at baseline, 97 (70.3%), 96 (69.6%), and 98 (71.0%) had at least one lesion with a discordant classification for the response assessment thresholds of ±20%, ±30%, and ±40%, respectively.Conversely, when the biomarker was held constant and the response assessment threshold was changed, the discordance in lesion-level classification ranged from 13.1 to 22.9%, depending on the imaging biomarker (see Table 5).

Patterns of progression
For all investigated biomarkers and response assessment thresholds, systemic progressors had a significantly higher risk of death compared to those with no progression (Fig. 3, Supplementary Table 6).After applying the Bonferroni correction to the p values for the 45 different hazard ratios calculated, systemic progressors still exhibited a significantly increased risk of death for all imaging biomarkers at each threshold, with the exception of tumour volume (p > 0.05 for all response assessment thresholds).Among the biomarkers, SUV mean consistently yielded the largest C-Index when comparing patients with systemic progression to those with no progression.In particular, SUV mean at the ± 30% threshold demonstrated the highest overall C-Index (HR = 5.70, 95% CI = 2.63-12.37,p < 0.001, C-Index = 0.69).Patterns of progression were further defined and analysed for the best performing biomarker (as measured by the C-Index), in this case the SUV mean at the ±30% threshold.Out of the total patient cohort, 85 patients (61.6%)   4), but not for mixed progression versus no progression (median OS not reached for both, p = 0.052).Supplementary Figs. 2 and 3 provide this analysis repeated for SUV max and the PERCIST-recommended biomarker of SUV peak on the highest uptake tumour region with a spherical volume of 1 mL (equating to approximately the radius of 6.25 mm used in this study).Baseline tumour burden was found to be statistically higher in patients with systemic progression relative to those with no progression (median of 4.76 mL vs. 1.69 mL, p = 0.049, Fig. 5a).This was not the case, however, for baseline PSA levels between systemic progressors and those with no progression (median of 5.3 ng/mL vs. 3.3 ng/mL, p = 0.22, Fig. 5b).There were  Results are presented for: (a) ± 20% lesion-level response assessment threshold for progressing and responding lesions, (b) ± 30%, and (c) ± 40%.In all cases, SUV mean yielded the equal or highest C-Index.Vertical dotted line is plotted at a hazard ratio of 1 for easy visual comparison.All p values are less than 0.05, with the exception of volume for all investigated thresholds (after correcting for multiple testing using the Bonferroni method).Completely responding lesions (i.e., lesions that were not identified on follow-up imaging), are here classified as "responding".The number of patients that are still at risk at a given time point, defined as those patients that have either not experienced death or been censored, are shown below plot (b) (time points in the table align with the x-axes of the plot).www.nature.com/scientificreports/no statistically significant differences for either the baseline tumour burden or baseline PSA values for all other group combinations (Fig. 5).Intra-patient lesion response heterogeneity was found to be substantial when analysed for the SUV mean biomarker at the ± 30% threshold.Of the 82 patients that presented with multiple disease sites at baseline imaging, a majority of them (49, 59.8%) had a mixture of responding, stable, and progressing lesions.Considering only the patients treated with systemic hormone therapy, 44% (33/75) had a mixture of classifications at the lesion level.The median tumour response percentage for the 328 matched lesions according to SUV mean was − 14.8% (range: − 91.1-884.9%).Bone lesions had the best response among lesion sub-types (median = − 20.4%), followed by nodal lesions (median = − 19.5%) and local prostate lesions (median = − 2.8%).Only bone and prostate lesions were found to have a significantly different tumour response distribution (p = 0.017).However, the differences in tumour response distributions between the other groups were not found to be statistically significant (p > 0.05 in pairwise comparison between all groups, Fig. 5c).A majority of the patients overall had at least one responding lesion (88/138, 63.8%), and this remained true when considering only patients with multiple disease sites at baseline (65/82, 79.3%).

Discussion
PSMA-targeted PET imaging is increasingly being used in response assessment settings for PCa patients, but the evidence of its prognostic utility in early disease BCR PCa patients is currently limited.One of the important advantages that whole-body molecular imaging confers to clinicians is the ability to assess progression at individual disease sites, which contrasts with biochemical progression measurements in the form of PSA, which have no ability to detect heterogeneous response across different disease sites 28 .In this work, we comprehensively  The SUV peak measurement used in this analysis is the PERCIST-recommended one-centered on the highest uptake part of the tumour with a spherical volume of 1 mL.The number of patients that are still at risk at a given time point, defined as those patients that have either not experienced death or been censored, are shown below each plot (time points in the table align with the x-axes of the plot).
of patient OS.To assess the prognostic ability of the biomarkers at each threshold, we characterised the progression dynamics according to pre-defined patterns of progression.Overall, regardless of the response assessment threshold chosen, the SUV mean had the equal or highest C-index for predicting decreased survival in patients with systemic progression compared to those without progression.The ±30% threshold performed the best, with an HR of 5.70 (95% CI = 2.63-12.37,p < 0.001) and a C-Index of 0.69.These findings suggest that SUV mean is a valuable biomarker for assessing lesion-level progression.However, it is important to note that all other biomarkers tested (except for volume after adjusting for multiple testing) also demonstrated significant increases in the risk of death for systemic progressors relative to patients without progression.Thus, despite the substantial heterogeneity found in lesion-level classifications, most biomarkers retain prognostic significance when these lesion-level classifications are packaged into patterns of progression, and considering other factors, this may lead to an alternative choice of biomarker for use depending on the clinical context.For instance, SUV mean , requires full delineations of each metastatic site to calculate, and while semi-automated and automated AI-based solutions exist for segmenting metastatic disease from PSMA PET scans [30][31][32] , not all centres around the world will have access to these technologies.This could make such an approach infeasible for patients with high disease burden.SUV max could be used as a more practical alternative in high disease burden cases, where the maximum voxel in a high uptake area can be localised without precise tumour delineations 33 .SUV max quantification in prostate lesions has demonstrated high inter-reader reproducibility, however, the use of the single highest voxel value in a region for tumour response quantification can be adversely affected by noise in the acquired image 34,35 .An alternative to SUV max is the PERCIST-recommended biomarker, SUV peak , which is more robust to noise due to volume averaging.However, response quantification using SUV peak is dependent on both the size of the spherical volume defining the region of interest and where that sphere is placed, as demonstrated not only in this study, but also an investigation by Vanderhoek et al. 25 .There is also the possibility that the specified region of interest over which the SUV peak is quantified might include voxels outside of the tumour volume.It's worth noting also that due to the limited spatial resolution of PET imaging, partial volume effects can affect biomarker quantitation accuracy 36 .This leads to an underestimation of lesion activity because of activity dilution at the borders of the region of interest from volume averaging, and makes the lesion appear larger than it is in reality.
A closer look at the patterns of progression for the SUV mean biomarker at the ±30% threshold illuminates more clinically relevant conclusions.Patients with systemic, mixed and no progression all had different survival outcomes, with systemic progressors demonstrating statistically lower survival probabilities than both patients with mixed (p = 0.017) and no progression (p < 0.001).This accords with the results of a study by Osorio et al. 21, that analysed lesion-level patterns of progression for two cohorts of patients, one with non-small cell lung cancer (NSCLC) and one with metastatic carcinoma with mismatch repair deficiency (MMRD) who were treated with programmed cell death protein (PD-1) blockade therapies.In both cohorts examined, mixed progressors were found to have improved OS relative to systemic progressors, although not to statistical significance in the MMRD cohort (p = 0.07).Notably, our analysis used the same definitions of progression patterns as this study, suggesting that they retain their prognostic significance across a range of metastatic cancer types and can be applied to PCa at the stage of BCR.Intra-patient heterogeneity of lesion-level response was also very common in patients with multiple disease sites, with a majority of them (59.8%)having a mixture of progressing, stable and responding lesions between imaging assessments.44% of patients who underwent systemic hormone therapy also presented with a mixture of classifications at the lesion level.This implies underlying biological heterogeneity between metastatic disease sites, of which previous studies have shown evidence for in PCa as well as other cancer types such as melanoma, NSCLC and gastric cancer 19,20 .Furthermore, a majority of patients in this study demonstrated response in at least one metastatic disease site.This finding highlights the potential importance of therapy continuation in cases where certain disease sites are responding favourably, even if other sites show limited response or progression.Such valuable information can only be captured with a lesion-level assessment of disease progression.It emphasizes the significance of incorporating such analyses in future studies and clinical trials to enhance our understanding of treatment responses and guide clinical decision-making.
This work also confirms the prognostic value of a radiographic interpretation of the PPP criteria (rPPP) for patient progression in the BCR setting using SUV mean , SUV max and the PERCIST-recommended SUV peak .The rPPP response framework validated in this work is consistent with the PSMA PET/CT response assessment consensus statement published by Fanti et al. 27 , where the presence of one new lesion in early recurrent PCa is enough to be considered PD.This work also adds evidence that confirms the value of the recently published PROMISE v2 framework which recommends the PPP be used for response assessment in PCa patients with limited metastatic disease, and we show that the assessment can be done purely radiographically while still maintaining prognostic value 37 .Furthermore, the prognostic value of the rPPP is maintained even when sub-grouping for just the patients that received anti-hormone therapy between scans.This sub-group analysis was conducted because of the growing evidence that androgen blockade can increase PSMA expression and affect PSMA PET quantitation 38,39 , potentially resulting in the conflation of true progression with receptor up-regulation in response to anti-hormone therapy.We acknowledge that this work does not account for this phenomenon, but note that the maintenance of prognostic value in the sub-group analysis is promising evidence that the rPPP framework can be used in the BCR setting even in such populations.Further subgroup analysis by treatment type also demonstrated that the rPPP remains highly prognostic for all treatment sub-groups for these three biomarkers with the exception of patients who underwent no therapy, suggesting that disease progression despite treatment is associated with a much poorer patient prognosis than progression when no treatment is administered.This conclusion should be tempered, however, by the relatively small number of patients in the no treatment sub-group (n = 39).Future prospective clinical trials with standardised treatment regimens are necessary to confirm these results.
This study has some limitations that should be noted.The retrospective nature of this work means that the findings warrant further prospective validation.It would be infeasible for such prospective clinical trials to evaluate all biomarkers-rather, the results of this work in combination with other pertinent considerations mentioned above can be used to narrow down a small number of candidate biomarkers.Such studies should also ideally incorporate delineations from more than one nuclear medicine physician if possible, as this was a limitation of the present work.Additionally, the patient cohort overall had relatively low disease burden PCa (median 2 lesions per patient) Therefore, the conclusions drawn from this study are applicable only to this cohort and may not extend to patients with more extensive disease burden.Further research is required to validate the prognostic utility of lesion-level analysis with respect to patient OS in more advanced disease populations.Indeed, it is possible that the patterns of progression definitions used in this work may need to be refined for patients with high disease burden.One can imagine a scenario, for example, where a patient with considerably advanced disease presenting with potentially ~ 100 disease sites is classified as having "systemic progression" in response to 177 Lu-PSMA therapy because a very small minority of those sites (say three) are unresponsive to therapy and increasing in uptake, even when the remainder may respond well.Response criteria incorporating overall tumour burden measurements, such as RECIP 1.0, might be more informative in such cases, as recommended by the PROMISE v2 framework 37 .Lastly, the patient cohort in this work were not administered a standardised treatment regimen, which limits the conclusions that can be drawn for individual treatment modalities.The overall conclusions about the prognostic utility of these patterns of progression and the rPPP will thus need to be verified for individual treatment modalities in future works, which would ideally be prospective.It is also possible that subsequent treatments administered to patients after the follow-up scan could confound the survival analysis conducted, and this is something that future works investigating the prognostic power of imaging metrics in this early disease setting should investigate.
In conclusion, evaluating radiographic response from 68 Ga-PSMA PET images in metastatic PCa at the lesion level is subject to significant heterogeneity among imaging biomarkers, which could have important implications for assessing therapy response at individual metastatic sites.However, despite this heterogeneity in lesion-level classifications, almost all biomarkers remained prognostically significant when patient-level patterns of progression were defined from these lesion-level classifications, with our results suggesting SUV mean is the most prognostically significant.The three progression groups had distinct survival outcomes, with systemic progressors at the highest risk of death, demonstrating the prognostic significance of lesion-level assessments in PSMA PET in the BCR setting.rPPP was also shown to be prognostic in this cohort, demonstrating that response assessment using PSMA PET is prognostically relevant even at early stages of metastatic PCa.Future prospective clinical trials should aim to validate the promising findings of this work.

Figure 2 .
Figure 2. Tumor response percentages, quantified as a percentage change in the respective biomarker between baseline and follow-up imaging, for: (a) SUV peak measurements which vary with type (centered on SUV max of the tumor, or the highest uptake region of the tumor) and radius of spherical volume (between 2.5 and 8.75 mm in increments of 1.25 mm), and (b) SUV mean , SUV max , and Volume.

Figure 3 .
Figure 3.Hazard ratios with 95% confidence intervals of patients classified as having systemic progression relative to patients classified as having no progression for each lesion-level imaging biomarker investigated.Results are presented for: (a) ± 20% lesion-level response assessment threshold for progressing and responding lesions, (b) ± 30%, and (c) ± 40%.In all cases, SUV mean yielded the equal or highest C-Index.Vertical dotted line is plotted at a hazard ratio of 1 for easy visual comparison.All p values are less than 0.05, with the exception of volume for all investigated thresholds (after correcting for multiple testing using the Bonferroni method).

Figure 4 .
Figure 4.In depth analysis of the properties of the SUV mean biomarker at the ± 30% response assessment threshold.Figure shows: (a) pie chart showing percentage of patients with each pattern of progression; Kaplan-Meier curves with annotated log rank p values for (b) systemic versus no progression, (c) mixed versus no progression, (d) systemic versus mixed progression, and; (e) waterfall plot showing the percentage of each patients' lesions classified in each lesion-level response category (progressing, stable, or responding).Completely responding lesions (i.e., lesions that were not identified on follow-up imaging), are here classified as "responding".The number of patients that are still at risk at a given time point, defined as those patients that have either not experienced death or been censored, are shown below plot (b) (time points in the table align with the x-axes of the plot). https://doi.org/10.1038/s41598-023-45106-2

Figure 5 .
Figure 5. Associations between patterns of progression categories as measured by best performing lesion-level biomarker, SUV mean , and (a) baseline tumour volume; and (b) baseline PSA values.No significant differences were found in tumour response between tumour subgroups of local prostate lesions, nodal lesions and bone lesions as measured by SUV mean (c).

Figure 6 .
Figure 6.Kaplan-Meier curves showing difference in survival probabilities between patients with PD and those without PD according to the rPPP.Analysis is conducted for: (a) SUV mean (b) SUV max and (c) SUV peak .The SUV peak measurement used in this analysis is the PERCIST-recommended one-centered on the highest uptake part of the tumour with a spherical volume of 1 mL.The number of patients that are still at risk at a given time point, defined as those patients that have either not experienced death or been censored, are shown below each plot (time points in the table align with the x-axes of the plot).
# Risk categories assigned based on gleason scores and PSA levels at referral.Low risk: PSA < 10 ng/mL and gleason score = 6.Intermediate risk: PSA between 10 and 20 ng/mL or gleason score = 7. High risk: PSA > 20 ng/mL or gleason score > 7. Risk categories not calculated for 3 patients with missing gleason scores.Continuous data is presented as the median with the range in parentheses, while nominal data is presented as the number with percentage of the whole in parentheses.PSA, Prostate specific antigen; ADT, Androgen deprivation therapy.

Table 4 .
Discordance of lesion-level progression classifications between measured quantitative biomarkers at each percentage change threshold.PR, Partial response.

Table 5 .
Discordance of lesion-level progression classifications between response assessment thresholds for each imaging biomarker investigated.PR, Partial Response; SUV, Standardised Uptake Value.