Evaluation of growth adaptation of Cinnamomum camphora seedlings in ionic rare earth tailings environment

The root system is an important organ for nutrient uptake and biomass accumulation in plants, while biomass allocation directly affects essential oils content, which plays an essential role in plant growth and development and resistance to adverse environmental conditions. This study was undertaken to investigate the differences and correlation of biomass allocation, root traits and essential oil content (EOC), as well as the adaptations of camphor tree with different chemical types to the ionic rare earth tailing sand habitats. Data from 1-year old cutting seedlings of C. camphora showed that the biomass of C. camphora cuttings was mainly distributed in root system, with the ratio of root biomass 49.9–72.13% and the ratio of root to canopy 1.00–2.64. The total biomass was significantly positively correlated with root length (RL), root surface area (RSA) and dry weight of fine roots (diameter ≤ 2 mm) (P < 0.05). Root biomass and leaf biomass were negatively and positively with specific root length (SRL) and specific root surface area (SRSA), respectively. Leaf biomass presented a positive effect on EOC (P < 0.05), with the correlation coefficient of 0.808. The suitability sort of these camphor trees was as follows: C. camphora β-linalool, C. camphora α-linaloolII, C. camphora α-linaloolI being better adapted to the ionic rare earth tailings substrate, C. camphora citral being the next, and C. porrectum β-linalool and C. camphora borneol being the least adaptive. EOC played a positive role in the adaptation of C. camphora (R2 = 0.6099, P < 0.05). Therefore camphor tree with linalool type is the appropriate choice in the ecological restoration of ionic rare earth tailings. The study could provide scientific recommendations for the ecological restoration of ionic rare earth tailings area combined with industrial development.

not clear is called C. camphor miscellaneous.According to previous researches, the same camphor tree could have different chemical types, such as camphor type, borneol type, 1,8-cineole type, α-rolinalool type, citral type, isonerolidol type, safrole type, and other chemotypes 6 .In addition, different camphor tree species may also have the same chemotype.For example, Cinnamomum camphora, Cinnamomum bodinieri, Cinnamomum porrectum, and Cinnamomum tenuipilum all have citral chemotypes 7 .At present, C. camphora are grown under dwarf cultivation in large areas in the southern provinces in China, and the aboveground parts of the stubble plants are extracted every year to extract essential oils.Therefore, the biomass allocation characteristics of C. camphora directly affect the production of essential oils.
Root system is an important organ for plants to absorb nutrients, playing a vital role in the biomass accumulation.Fine root (diameter ≤ 2 mm) traits (root length (RL), root surface area (RSA), specific root length (SRL), etc.) have proven to be important indicators of root morphology and structure, ecological adaptability and resource acquisition capacity.These indicators play a crucial role in nutrient uptake and transportation [8][9][10] .It has been shown that root architecture is strongly influenced by the environment and adapts to changing environments through root plasticity 11,12 .Plants with high carbon investments in poor soils allocate more biomass to fine roots for root synthesis and access to nutrients by increasing RL 13,14 , while plants with low carbon investments uptake through morphological and physiological plasticity of the root system 15 , implying a trade-off between resource foraging and resource conservation 14 In contrast, under eutrophic conditions, plants absorb soil nutrients by increasing fine root SRL and RSA and decreasing RL 16,17 .Some studies also showed that root dry weight, fine root RL and fine root RSA were all significantly and positively correlated with yield in soybean at bulging stage under water-nitrogen treatment 18 .Fast-growing species have higher SRL and lower root tissue density than slowgrowing species 19 .These inconsistent results may be related to soil infertility and plant species.
The chemical composition and structure of the soil strongly affect the growth and development of plants.Ecophysiologically, the effects of soil are translated in changes in plant primary and secondary metabolism (essential oils) 20 .Therefore it is necessary to screen the chemotypic camphor species with high adaptability and high essential oil content (EOC) for the ionic rare earth mine tailings area.On this basis, we provide the following assumptions: (1) there are diversity in the root morphological characteristics of camphor tree seedlings with different chemotypic essential oils in ionic rare earth backfilling matrix; (2) root morphological characteristics (such as RL, RSA, SRL, etc.) play a vital role in the biomass allocation of camphor tree seedlings; (3) the essensial oils in camphor tree seedlings help enhance the adaptability of plants to ionic rare earth backfilling.In order to solve the above assumption, we compared and analyzed the biomass allocation, root traits and EOC of 7 different chemical types of camphor tree seedlings in the ionic rare earth tailings matrix, and illustrated the relationship between root morphology and biomass allocation of camphor tree seedlings, and comprehensively analyzed the ecological and economic adaptations of different chemical camphor trees.The results of the study could provide scientific recommendations and management strategies for the ecological restoration of ionic rare earth tailings combined with industrial development.

Plant growth status
The plant height of No. 5 and No. 6 camphor trees was significantly higher than that of No. 3 and No. 7 camphor trees, while there was no significant difference with miscellaneous camphor trees.Camphor No. 1, No. 5 and No. 6 had 32.5-60.3%higher crowns than the other chemotypes (Table 1).

Biomass allocation
The leaf biomass in No. 5-7 C. camphora was significantly higher than that of No. 1-3 camphor trees.The No. 1 C. camphor miscellaneous had the most stem, root and total biomass, followed by No. 4-6 camphor trees and No. 7 C. porrectum β-linalool was the lowest, in which the highest was 5.44, 3.96 and 3.91 times the lowest (Table 2).
In terms of biomass distribution, the biomass of all camphor trees was mainly distributed in the root system, with a distribution ratio of 49.9-72.13%.The biomass in leaf accounted for 2.41-9.81%and that in stem accounted for 24.09-45.08%.Among them, the highest proportions of root biomass and leaf biomass were No. 1 camphor, and No. 7 C. porrectum β-linalool, respectively (Fig. 1).The root-to-shoot ratios of the 7 species of camphor trees ranged from 1 to 2.64, with that in No. 1 C. camphor miscellaneous was the highest (Table 2).Table 1.Growth status of camphor seedlings.Each value represents the mean ± SE.Means within a column followed by the same letter(s) are not significantly different (P > 0.05).

Root weight
The dry weight of fine roots and coarse roots of the No. 1 C. camphor miscellaneous was significantly higher than that of other camphor trees, and the weight of fine roots was about 3-5 times that of other camphor trees.
There was no significant difference between the weights of fine roots of other camphor trees.In terms of finecoarse root weight ratio, that of No. 1, 3, 5, 6, and 7 camphor trees ranged from 0.91 to 1.53, with no significant differences (Table 4).

SRL and SRSA
The SRL and SRSA reflect the ability of plants to absorb nutrient resources.The SRL of No. 2, No. 3, No. 5, and No. 6 camphor trees was significantly higher than that of No. 1, No. 4 and No. 7 camphor trees, with the ratio 36.8-110.9%.Among them, the fine root of the No. 5 camphor tree had the largest SRL.For the ratio of SRL and SRSA varied in the range of 87.88-400.6 and 43.56-105.15,respectively.The SRSA of the fine roots and the whole roots of No. 3 and No. 5 camphor trees were significantly higher than those of other camphor trees (Table 5).

EOC
With the exception of C. camphora miscellaneous, the oil yield of the leaves of the six different chemotypes of camphor was 0.42-2.88%,and the EOC was 2.25-3.02mg/plant.The highest EOC was found in chemotypes 5 and 6, which were 8.2%, 11.6% and 30.2%, 34.2% higher than chemotypes 2 and 3, respectively.All the above shows that the EOC of linalool-type camphor is relatively higher in the ionic rare earth tailing sand matrix (Table 6).

Relationship between plant biomass, EOC and root traits indexes
There was a significant and positive correlation between the total biomass (TB) and several root characteristics, including RL, RSA, and root biomass (RB) (P < 0.05) as well as stem biomass (SB) (P < 0.01).Both RSL and SRSA exhibited a negatively effect on RB and a positive effect on leaf biomass (LB) (Fig. 2).The results show that camphor mainly accomplishes biomass accumulation by establishing root system, extending RL and expanding RSA, rather than changing root system diameter to achieve this purpose.Through the above process, camphor tree completed the adaptation to the ionic rare earth tailings environment.Principal Component Analysis was conducted to evaluate the relationship between plant biomass, EOC and root traits index (Fig. 2).PCA1 accounted for 88.88% of the variations, while PCA2 accounted for 7.04%.Of the Table 4. Biomass of Fine and coarse roots in camphor trees with different chemical types.Each value represents the mean ± SE.Means within a column followed by the same letter(s) are not significantly different (P > 0.05).

Chemical type
Root biomass (g)

Fine-coarse root weight ratio Fine root
Coarse root

Relationship between adaptive ranking and EOC
Different chemotypes of camphor were ranked in order of their adaptability to ionic rare earth tailings from high to low (1-6), and the serial numbers were plotted against the essential oil content as shown in Fig. 3.It showed that as the adaptability decreased, the EOC decreased, and the two indicators were significantly and linearly correlated with each other (P < 0.05) (Supplementary Information).

Discussion
Biomass accumulation indicates the ability of plants to utilize and accumulate space resources, and its distribution characteristics can reflect adaptation strategies of plant to environment [21][22][23] .In this study, the soil structure of the ionic rare earth tailings was damaged, and the water retention capacity was poor, as well as the nutrients  were poor 24 .Thus, plants were under severe water and nutrient stress.All camphor trees had the highest biomass in the root, followed by stems, and the leaf had the lowest biomass (Table 2, Fig. 1), indicating that camphor tree seedlings allocated more resources to the root system to obtain nutrients in order to cope with the unfavorable conditions of ionic rare earth tailings.Among the tested plants, the roots and total biomass of C. camphor Miscellaneous were significantly higher than those of other chemotype camphor trees (Table 2), indicating that the C. camphor Miscellaneous had better adaptability and growth potential to the ionic rare earth tailings matrix by increasing root length, root surface area and root biomass, and dedicating more resources to root system construction to improving adaptation to the ionic rare earth tailings environments.It is mainly because these essential oils are secondary metabolites of camphor tree, and the synthetic precursors from carbohydrates produced by photosynthesis, mainly affects the synthesis and accumulation of secondary metabolites by affecting the synthesis and accumulation of primary metabolites 25 .Therefore, the accumulation of secondary metabolites in camphor tree with typical chemical substances consumes some photosynthetic products, and the substances used for plant biomass accumulation and distribution would be limited [26][27][28] .For this reason, the biomass accumulation of all chemical camphor trees was lower than that of C. camphor Miscellaneous.It was shown that the root proportion and root-to-shoot ratio of C. camphora citral and C. porrectum β-linalool were significantly higher than those of C. camphora α-linalool and C. camphora β-linalool (Fig. 2), indicating that in the ionic rare earth tailings matrix, the growth of C. camphora citral and C. porrectum β-linalool may be severely stressed.As a result, the plants used more resources for building root systems and improved the adaptability of plants to ionic rare earth tailings by increasing the root-to-shoot ratio [29][30][31] , which was consistent with Wang et al. 32 on the anatomical structure characteristics and drought resistance characteristics of leaves of camphor trees for different chemical types of oil.The drought resistance of C. camphora citral and C. porrectum β-linalool was lower than that of linalool (α-linalool and β-linalool type), and they were vulnerable to drought stress under the condition of low water retention in ionic rare earth tailings.The proportion of leaf biomass in C. porrectum β-linalool was significantly higher than in other camphor trees (Fig. 2), mainly because the leaf area of sassafras (C.platyphyllum) was larger than that of other chemotype camphor trees, which required more resources for leaf growth and photosynthesis and promoted the accumulation of leaf biomass, which was another reason for weaker adaptivity in C. porrectum β-linalool than other chemical type camphor.
Most studies have shown that the more biomass allocated to fine roots indicates that the root system can better absorb nutrients, potentially increasing the ability of plants to adapt to the soil environment [33][34][35] .In the current study, the large differences in the biomass of different tissues and their percentage, as well as in the root indexes among different chemotypes of camphor (Tables 2, 3, 4, 5) indicated that there was great variance in the adaptability of these camphor trees to the ionic rare earth tailing sand substrate due to the factors of plant species and chemotypes.The PCA results showed that total biomass was significantly and positively correlated with RL, RSA and dry weight, while root biomass and leaf biomass were negatively and positively correlated with fine root SRL and SRSA, respectively.The RL, RSA, root weight and SRSA of the fine roots of C. camphora citral, C. camphora α-linaloolIand C. porrectum β-linalool are the lowest, which limited the use of carbon sources in the soil 36 , In comparison with C. camphora α-linalool I and C. porrectum β-linalool, C. camphora citral with higher SRL of fine root had a higher capacity to absorb nutrients in soil 31 .However, C. camphora citralr transported absorbed nutrients to the leaves for photosynthesis, and root system construction is compromised, making it the least adapted to ionic rare earth tailings.
The composition of essential oils with different chemotypes is an expression of biodiversity within plant species 37 .As the secondary metabolites, essential oils play an important role in plant growth and development and resisting external adverse environment 38 .Previous studies have shown that the secondary metabolite essential oil produced by camphor belongs to terpenes, and the biosynthetic pathway of terpenes is usually divided into www.nature.com/scientificreports/three stages: the formation stage of C5 precursor isopentenyl diphosphate (IPP) and its double bond isomer dimethylallyl diphosphate (DMAPP); The generation stage of direct precursors (farnesyl diphosphate FPP, tauryl diphosphate GPP, tauryl tauryl diphosphate GGPP, etc.) 39 ; Terpene formation and modification stage (redox, acylation, glycosylation, etc.).Among the three stages, the first two stages are shared by all terpenoids, and the third stage determines the structural diversity of terpenoids 40 .It could be seen that differences in the formation pathways of essential oils from different chemotypes of camphor 41 .In addition, the adaptability of C. camphora citral to ionic rare earth tailings was lower than that with linalool type and borneol type, which might be due to the difference in metabolic pathways and energy consumption of secondary metabolites, such as citral, borneol and linalool produced by camphor trees, so that camphor trees with different chemical type had differences in tissue construction and biomass distribution.However, the pathway of natural secondary metabolites in camphor tissue is not clear, which needs to be further revealed.
C. camphora in this study enriched with different types and contents of essential oils (Table 6), and their adaptability to the ionic rare earth tailings substrate increased with increasing essential oil content (Fig. 3).Lajayer et al. (2017) ***made similar observations that low levels of copper and zinc stress promoted carbon sources utilised in the terpene biosynthetic pathway, may positively influence essential oil quality and quantity [42][43][44] .In some cases, stressed plants are induced to change the quantity and quality of their secondary metabolites, such as phenolic compounds and essential oils, in order to cope and adapt to the concomitant environment.All of the above suggests that the amount of essential oil content may be one of the strategies used by Camphor to cope with ionic rare earth tailings habitats.
In summary, the adaptation of different chemotypes of camphor to ionic rare earth tailings sand habitats is a complex process that requires biomass partitioning between above-and below-ground tissues and among different organs, and even EOC.Our experimental results confirmed the pre-hypothesis.The conclusions were as follows.Camphor accumulates total biomass by increasing RL, RSA and dry weight, and increases the capacity of fine roots to absorb nutrients by altering root diameter indexes (SRL, SRSA), which is used for leaf biomass accumulation aboveground.Preliminary evaluation of the adaptability of different chemotypes of camphor seedlings in ionic rare earth tailings sand resulted in C. camphora β-linalool, C. camphora α-linalool II and C. camphora α-linalool I being better adapted to the ionic rare earth tailings sand substrate, C. camphora citral being the next best, and C. porrectum β-linalool and C. camphora borneol being the least well adapted.Plant adaptation is positively correlated with plant EOC, i.e., an increase in the EOC of camphor had a positive effect on its adaptation to ionic rare earth tailings.Therefore, C. camphora with linalool type could be considered as economic and industrial trees in the vegetation restoration in combined with industrial development in the ionic rare earth tailings area.

Experimental materials
The tested plants were 1-year-old Cinnamomum camphora cuttings of excellent chemotypic monocots prescreened by the subject group of Jiangxi Province Engineering Research center of Seed-breeding and Utilization of Camphor Trees.The group collected wild camphor seeds from the southern provinces of China (wild distribution area of Cinnamomum camphora) in 2018.In the second year, the seeds were sown to obtain live seedlings of different seed sources, after which the leaves of the live seedlings were collected.Then the phytochemical type was determined by the odor method, and the essential oil was extracted.The relative content of the main components in the leaf essential oil was detected by the water distillation method and the gas chromatography-mass spectrometry method.Finally, the 3-year old live seedlings of Camphoria with a high EOC and high content of the main components were taken as cuttings with the 1-year old semi-lignified branches.The cuttings were finally obtained and used as test materials for this experiment.The tested plants were sourced in compliance with relevant institutional, national and international guidelines and legislation.
The tested soil was obtained from an abandoned ionic rare earth mine in Xunwu County, Jiangxi Province in China.The physical and chemical data of the tested ionic rare earth tailings were as follows: soil pH was 5.08 (strongly acidic); the conductivity was as high as 79.12 dS/m; the soil organic carbon, organic matter, total nitrogen, and total phosphorus contents in the tailings were 6.29 g/kg, 10.85 g/kg, 0.20 mg/kg and 1.21 g/kg, respectively; and the available phosphorus and available potassium contents were 13.78 mg/kg and 225.97 mg/ kg, respectively.

Experiment method
The experiment was carried out in the Biotechnology Test Base of Nanchang Institute of Technology in Nanchang, Jiangxi Province in China.The base belongs to the subtropical humid monsoon climate, with annual rainfall of 1600-1700 mm, rainy season from April to June, seasonal drought period from July to September, annual average relative humidity of 78.5%, and annual sunshine duration of 1723-1820 h.
Based on the excellent camphor tree species screened by the research group based on "three highs" (high biomass, high essential oil content, and high content of main components), 1-year-old cutting seedlings of camphor trees for each chemical type (about 15 cm in height, 12 cm in average crown width, and about 5.5 mm in basal diameter) were selected and planted in pots (29 cm in diameter, 26 cm in height) in March 2020.Each pot was filled with 8 kg of ionic rare earth tailings matrix, and there were 10 pots of camphor trees for each chemical type, totaling 70 pots.The initial stage of the experiment was in the local rainy season, and the potted plants were grown under natural conditions.During the high temperature and dry period in July and August, the water was completely replenished every 10 days, and the water was sufficient when it flowed out from the bottom of the pots.

Biomass determination
At the end of the plant growing season, all the plants for each chemotype were harvested, and the plant height and crown width were measured.We removed the aerial parts of the plants uniformly, and then divided each camphor tree into stems and leaves and put them into an envelope and marked them, respectively.The samples were baked at 80 °C to constant weight to obtain the leaf and stem biomass of the plant.The sum of the leaf and stem biomass is called the above-ground biomass.

Root trait determination
The whole plant was excavated using the "whole root method".Along the direction of each root, we used tweezers and a small shovel to get the whole root system of the plant, rinsed it with clean water in a mesh bag, and divided the root system into fine roots (diameter ≤ 2 mm) and coarse roots (diameter > 2 mm).Through the root analysis system WinRHIZO Pro software (Reagent Instruments, Quebec, Canada), the RL and RSA of the plants were obtained.Afterward, the roots were scanned, put into envelopes, and baked at 80 °C to constant weight.The ratio of root length to root weight is called SRL, and the ratio of RSA to root weight is called SRSA.The sum of biomass of fine and coarse roots is called the underground biomass.

Essential oil content determination
All leaves of camphor tree were collected and grouped in November according to chemical type, and then took about 200 g fresh samples of the leaves from different chemical types.The essential oil was obtained by steam distillation through the patented product "Portable camphor essential oil steam distiller" developed by Jiangxi Province Camphor Breeding and Development and Utilization Engineering Research Center of Nanchang Engineering College.At the same time, the water content of leaves was measured quickly by a rapid moisture meter (Sartorius MA150).The amount of essential oil/dry mass of leaf biomass was used as the index of essential oil content and the essential oil yield of the total yield per plant were also calculated based on the dry mass per plant 29,45,46 .

Statistical analysis
One-way analysis of variance (one-way ANOVA) was used to compare the significance of differences in growth status, biomass distribution, and root traits of camphor tree seedlings with different chemotypes.The Duncan method was used for multiple comparisons, and the significance level was set at P < 0.05.All data were reported as mean ± SE from 10 plants per chemical type of camphor tree.Correlations between the plant biomass and the root trait indexes, and that of the essential oil content with the biomass and root morphology indexes were obtained by Pearson's analysis, and the analysis results were shown in the form of an annex.The principal component analysis (PCA) was used to comprehensively evaluate the relationship between biomass allocation indexes, root traits parameters and EOC of plants, as well as the adaptive of plants to the ionic rare earth tailings.The multiple linear regression was used for analyzing the relationship between EOC with the biomass allocation and root traits.All analyses were conducted using SPSS 14.0 (SPSS Inc., Chicago, USA) and figures were generated by Origin 8.0 (OriginLab Corporation, Northampton, MA, USA).

Figure 1 .
Figure 1.Biomass allocation ratio of different organs in camphor seedlings with different chemical types.

Figure 2 .
Figure 2. PCA (principal component analysis) among the plant biomass, root traits indexes and essential oil content in camphor seedlings of different chemical types.TB total biomass, SB stem biomass, SR stem ratio, LB leaf biomass, LR leaf ratio, RB root biomass, RR root ratio, EOC essential oil content, FRL fine root length, CRL coarse root length, RLR root length ratio of fine root to coarse root, FRSA fine root surface area, CRSA coarse root surface area, SRSA specific root surface area, FRW fine root weight, CRW coarse root weight, RWR root weight ratio of fine root to coarse root, SFRL specific fine root length, SCRL specific coarse root length, SRLR specific root length ratio of fine root to coarse root, SFRSA specific fine root surface area, SCRSA specific coarse root surface area, STRAS specific total root surface area.The blue arrows represent plant biomass and essential oil content, the gray arrows represent root traits.

Table 2 .
Biomass allocation characteristics of camphor seedlings with different chemical types.Each value represents the mean ± SE.Means within a column followed by the same letter(s) are not significantly different (P > 0.05).

Table 3 .
Root length and root surface area of camphor seedlings of different chemical types.Each value represents the mean ± SE.Means within a column followed by the same letter(s) are not significantly different (P > 0.05).RL root length, RSA root surface area, ratio of RL was the ratio of fine root length to coarse root length, ratio of RSA the ratio of fine root surface area to coarse root surface area.
root length and specific surface area of camphor seedlings of different chemical types.Each value represents the mean ± SE.Means within a column followed by the same letter(s) are not significantly different (P > 0.05).SRL specific root length, SRSA specific root surface area, Ratio of SRL the ratio of SRL of fine root to coarse root.Vol.:(0123456789)Scientific Reports | (2023) 13:16910 | https://doi.org/10.1038/s41598-023-44145-zwww.nature.com/scientificreports/evaluated attributes, the coarse root weight (CRW) had significant conditional effects (P < 0.05), while the fine root surface area (FRSA) had extremely significant effects (P < 0.01).

Table 6 .
Essential oil content of camphor trees with different chemical types.Each value represents the mean ± SE.Means within a column followed by the same letter(s) are not significantly different (P > 0.05).EOC essential oil content.