Comparison of 2-octyl cyanoacrylate and n-octyl cyanoacrylate topical skin adhesives for wound closure after ankle fracture surgery: a prospective randomized trial

To date, only a few clinical studies have investigated the differences between 2-octyl cyanoacrylate and n-octyl cyanoacrylate topical skin adhesives (TSAs). This study aimed to compare the outcomes of the two TSAs for wound closure after ankle fracture surgeries. Fifty-six patients were randomized to receive either a 2-octyl or n-octyl cyanoacrylate TSA. At 3 and 6 months after surgery, wound cosmetic outcomes were assessed using the Hollander Wound Evaluation Scale (HWES), and patient satisfaction for wound cosmesis was assessed using the visual analog scale (VAS) and 5-item Likert scale. Functional outcomes at 6 months after surgery were assessed using the Olerud–Molander Ankle Score (OMAS). Fifty-five patients completed the study protocol. Within the follow-up period, no differences were found between the two groups in terms of HWES, VAS, 5-item Likert scale, and OMAS. 2-octyl cyanoacrylate TSA and n-octyl cyanoacrylate TSA were comparable options for wound closure after ankle fracture surgeries in terms of wound cosmesis, patient satisfaction, and functional outcome.


Inclusion/exclusion criteria
Patients who required open reduction and plate fixation for isolated lateral malleolar fractures were included.Among the patients with rotational ankle fractures, those with associated posterior malleolar fractures or a partial deltoid ligament rupture but only required open reduction and plate fixation for the lateral malleolar fracture were also included.The exclusion criteria were as follows: (1) < 18 or > 65 years of age; (2) previous surgeries at the ankle joint; (3) significant medical comorbidities potentially affecting wound healing (e.g., end-stage renal failure, peripheral vascular disease) 17,18 , (4) history of keloid formation, open fracture, or other notable skin injuries around the ankle joint combined with fracture; (5) blistering on the incision site; (6) > 10% of ankle edema in the injured side compared to the uninjured side measured by the figure-of-eight-20 method 19 , (7) workers' compensation, and (8) participation in another study.Among the eligible patients, those who provided informed consent for participation were finally included.

Participants
Between April 2018 and May 2021, 342 patients visited our institution for rotational ankle fracture, and 78 patients met the inclusion/exclusion criteria.Of these patients, 22 were excluded because they did not wish to participate in the study, leaving 56 patients randomized into both groups (Fig. 1).The full date of the first registration was 20/08/2021, and the registration number was KCT0006476.

Randomization
Patients were randomly assigned to one of the two groups using sequentially numbered opaque-sealed envelopes made by the study coordinator.The randomization sequence was constructed using computer software (Excel 2016; Microsoft, Redmond, WA, USA) 20 .Neither the patients nor the surgeons knew the randomization outcomes until the day of surgery.

Preoperative assessment
Preoperatively, 3 to 7 days of bed rest and elevation of the injured ankle were performed to reduce swelling to an acceptable level for internal fixation.To quantify preoperative soft tissue swelling at the incision site, both ankles were measured using the figure-of-eight-20 method 19 .The percentage of measured edema on the injured side was calculated as a fraction of the edema of the injured side to that of the uninjured side, and this value was used to identify intergroup differences.

Interventions
Open reduction and plate fixation of the fractures were performed by a single surgeon on the basis of standard AO principles 21 .Closure of the fascial and subcuticular skin layers was performed in the same way in both groups using 2-0 and 3-0 synthetic absorbable braided sutures (Vicryl Plus, Ethicon Ltd, Edinburgh, UK).Either 2-octyl cyanoacrylate TSA (Dermabond Prineo, Ethicon, Somerville, NJ, USA) or n-butyl cyanoacrylate TSA (Liq-uiBand, Advanced Medical Solutions, Winsford, UK) was then applied in two thin layers with a drying time of 30-60 s between layers to enable polymerization.In the 2-octyl cyanoacrylate group, this process was performed over the contained polyester mesh according to the manufacturer's instructions.A standard absorbent dressing was then applied and changed on the second day after surgery.Polyester mesh in the 2-octyl cyanoacrylate group was removed 14 days after surgery, as per the manufacturer's instructions.

Primary and secondary outcomes
As a validated wound assessment tool, the Hollander Wound Evaluation Scale (HWES) was used as a primary outcome measure.The HWES consists of five subscales, and the wounds with a score of 6 were considered to have an optimal cosmetic appearance: step-off of borders (edges not on the same plane; 0 for yes, 1 for no), contour irregularities (wrinkled skin near the wound; 0 for yes, 1 for no), margin separation (gap between side; 0 for yes, 1 for no), edge inversion (wound not properly everted; 0 for yes, 1 for no), excessive distortion (swelling/edema/ infection; 0 for yes, 1 for no), and overall appearance (subject appearance: 0 for poor, 1 for acceptable) 22,23 .The assessment of HWES was independently performed by two orthopedic surgeons, and in cases of disagreement between them, the final decision was made via consensus.
Secondary outcome measures included the visual analog scale (VAS) and 5-item Likert scale to assess patient satisfaction with wound cosmesis.The VAS score was determined by measuring the distance (mm) on the 10-cm line between the "worst possible scar" anchor and the patient mark, providing a range of scores from 0 to 100 24,25 .On a 5-point Likert scale, patients were asked about their satisfaction with wound cosmesis in one of the following items: very unsatisfied, unsatisfied, neutral, satisfied, and very satisfied 26 .The HWES, VAS, and 5-point Likert scale were assessed at 3 and 6 months of follow-up visits.To reduce the bias of outcome due to the incision length, the incision lengths were measured to investigate whether there was a difference between the two groups.
Additionally, the Olerud-Molander Ankle Score (OMAS; scale, 0-100 points) was assessed at 6-month followup visits to measure the functional outcome.A higher OMAS indicates better outcomes and fewer symptoms 27 .

Blinding
A single-blinded study design was used.Surgeons and patients were not blinded to group allocation.Outcome assessments and statistical analyses were performed by one author who did not participate in the operative procedures and was blinded to the allocations.

Sample size calculation
The non-inferiority trial method was used to determine the appropriate sample size 28 .Using the HWES at 6 months postoperatively as the primary outcome of measure, the sample size was calculated to have a power of 80% and a significance level of 0.05.To determine whether the 2-octyl cyanoacrylate TSA was not inferior to the n-butyl cyanoacrylate TSA, 25 patients per group were required to have 80% power that the lower limit of a one-sided 95% confidence interval for the difference between two treatments would be above the non-inferiority margin of −1.0 point (standard deviation, 1.25 points).Since there was no universally established minimal clinically important difference for the HWES, the non-inferiority margin was selected by investigators on the basis of a previously conducted prospective study 29 .Adding an assumed dropout rate of 10%, 28 patients were finally enrolled in each group.www.nature.com/scientificreports/

Statistical analysis
The Kolmogoro-Smirnov test was used to determine data normality.The independent t-test or Mann-Whitney U test was used to compare the following continuous variables: age, body mass index (BMI), HWES score, VAS score, OMAS, and incision length.The chi-squared test or Fisher's exact test was used to compare the following categorical variables: sex, smoking status, diabetes, injury mechanism, and 5-point Likert scale score.Interobserver reliability for the HWES was assessed by calculating the kappa coefficient (κ), and the values were judged as follows: poor (< 0.0), slight (0.01-0.20), fair (0.21-0.40), moderate (0.41-0.60), substantial (0.61-0.80), and almost perfect (0.81-1.00) 30 .All statistical analyses were performed using SPSS (version 23.0;IBM Corp, Armonk, NY), with statistical significance set at p < 0.05.

Patient characteristics
Fifty-five patients completed 6 months of follow-up.There were no intergroup differences in terms of age, sex, BMI, smoking status, diabetes, injury mechanism, ankle edema, or incision length (Table 1).

Secondary outcome analysis
The VAS and 5-point Likert scale scores for patient satisfaction with wound cosmesis did not show any statistically significant differences between the two groups at 3 and 6 months after surgery (Figs. 3 and 4).The 6-month postoperative OMAS was 73.6 ± 16.5 and 72.9 ± 14.5 in the 2-octyl cyanoacrylate and n-butyl cyanoacrylate groups, respectively, with no statistically significant difference (p = 0.864).

Complications
Except for one patient in the 2-octyl cyanoacrylate group who had a superficial wound infection by methicillinsensitive Staphylococcus aureus, no postoperative complications were reported.The patient was treated with oral antibiotics and repeated dressing, and the wound healed without sequelae.www.nature.com/scientificreports/

Discussion
Despite the increasing use of 2-octyl and n-butyl cyanoacrylate TSAs for wound closure in orthopedic surgeries, comparative studies on clinical outcomes after using both materials are limited.We found no difference between the two materials in terms of wound cosmesis, patient satisfaction, and functional outcome.Despite showing inferior mechanical properties, the clinical outcomes of n-butyl cyanoacrylate TSA are not inferior to those of 2-octyl cyanoacrylate TSA.Therefore, clinicians may freely choose either material, according to their preference, the wound size, and cost difference.
Although several animal studies demonstrated clear differences in the mechanical properties between the 2-octyl cyanoacrylate TSA and the n-butyl cyanoacrylate TSA, there were no differences in the clinical outcomes evaluated through this study.To explain this discrepancy, we deduced the following two possibilities.First, unlike animal studies that evaluated the wound bursting strength and tensile strength immediately after closure, the cosmetic wound assessments in this study were performed at least 3 months after surgery.This time interval may be sufficient for scar remodeling, diluting the cosmetic differences caused by the mechanical properties of the two materials.Second, various stretching forces acting on the incision site of the lateral malleolus in this study may have been below the threshold that could reflect the difference between the mechanical properties of the two materials.To clarify these possibilities, future studies should be conducted in the early phases of surgery with incision sites that are more vulnerable to tensile forces, such as total knee arthroplasty.
The strengths of this study are that it was designed prospectively, used a validated wound assessment tool, and applied TSAs to wound closure in fracture surgery.In our previous study 13 , we retrospectively compared the outcomes of 2-octyl cyanoacrylate TSA, n-butyl cyanoacrylate TSA, and nylon sutures for wound closure in Achilles tendon rupture repair surgery, and found no differences in patient satisfaction and complications between the two cyanoacrylate TSAs.However, the validity of the outcome measure was questioned.In addition, via a prospective randomized clinical trial, Osmond et al. 14 compared 2-octyl cyanoacrylate TSA and n-butyl cyanoacrylate TSA.They used the HWES to assess wound cosmesis and found no intergroup differences.However, applying these results to orthopedic surgery was limited because the patient cohort was confined to children with facial lacerations of < 4 cm.Therefore, we believe that this study will have a high clinical utility by complementing previous studies.
This study had two limitations.First, the LiquiBand used for the n-butyl cyanoacrylate TSA group was a blend of 90% n-butyl cyanoacrylate and 10% 2-octyl cyanoacrylate.Compared to the 2-octyl cyanoacrylate TSA group, in which 100% 2-octyl cyanoacrylate was used, using 10% 2-octyl cyanoacrylate in the n-butyl cyanoacrylate TSA group may have biased the results of the study.We decided to use LiquiBand because there was no commercially available monomeric n-butyl cyanoacrylate TSA product at the time of the study in our country.However, considering the mechanical study that reported no differences in wound bursting strength and tensile strength between LiquiBand and monomeric n-butyl cyanoacrylate TSA (Histoacryl) 12 , we believe that the use of LiquiBand would not significantly affect the results.Second, the incidence of complications was not compared.Since the complication rates following the application of TSAs were low 31,32 , comparing the incidence of complications will likely show no statistically significant differences between the groups, whether a true difference exists (i.e., a type 2 error).Therefore, considering the sample size of this study, we did not compare the incidence of complications.
In conclusion, of n-butyl cyanoacrylate TSA for wound closure after ankle fracture surgeries was not inferior to 2-octyl cyanoacrylate TSA in terms of wound cosmesis, patient satisfaction, and functional outcome.These findings suggest that, like 2-octyl cyanoacrylate TSA, n-butyl cyanoacrylate TSA is a viable alternative to traditional wound closure methods in orthopedic surgery.

Figure 1 .
Figure 1.Flow diagram of the study.TSA topical skin adhesive.

Figure 3 .
Figure 3. Visual analog scale scores for patient satisfaction to wound cosmesis.No significant difference was found between the 2-octyl cyanoacrylate TSA (blue) and n-octyl cyanoacrylate TSA (green) groups at 3 and 6 months after surgery (p = 0.659 and p = 0.700, respectively).TSA, topical skin adhesive.

Figure 4 .
Figure 4. 5-point Likert scale scores for patient satisfaction to wound cosmesis.No significant difference was found between the 2-octyl cyanoacrylate TSA (blue) and n-octyl cyanoacrylate TSA (green) groups at 3 and 6 months after surgery (p = 0.915 and p = 0.971, respectively).TSA topical skin adhesive.

Table 1 .
Patient characteristics.Values are presented as means ± standard deviations or number of patients.BMI body mass index, MVA motor vehicle accident.

Table 2 .
Hollander wound evaluation scale.Values are presented as means ± standard deviations.