Meat hygiene knowledge, handling practices and associated factors among meat handlers in Gedeo zone, Ethiopia

A cross-sectional study was conducted among 239 randomly selected meat handlers working in butcher shop in southern Ethiopia to assess factors associated with meat hygiene knowledge and practices. A binary logistic regression analysis with a 95% confidence interval (CI) and a p-value < 0.05 was used to identify factors that were significantly associated with good level of meat hygiene knowledge and practices. The findings revealed that 38.5% [95% CI: 32.2–44.8%] and 25.1% [95% CI: 19.7–30.5%] of meat handlers have good levels of meat hygiene knowledge and practices, respectively. Good level of meat hygiene knowledge was significantly (p < 0.05) associated with educational level, having meat hygiene training, and having regular supportive supervision by health workers, whereas good level of meat handling practice was significantly associated with work experience, educational level, have regular supportive supervision by health professionals, and having meat hygiene training. In conclusion, the majority of meat handlers have poor knowledge and practices regarding meat hygiene among meat handlers. Educational level, meat hygiene training, and supportive supervision by a health professionals were all independent predictors of meat hygiene knowledge and practice among meat handlers. As a result, health professionals should give regular training, butcher shop inspections, and supportive supervision for meat handlers in order to improve meat hygiene knowledge and practices among meat handlers.


Materials and methods
Study design, setting and period.A cross-sectional survey with structured observation was conducted among butcher shop meat handlers in Dila town, Gedeo zone, southern Ethiopia, from June 5 to July 15, 2022.Dilla town is the capital of Gedeo zone and is located 365 km south of Ethiopia's capital city, Addis Ababa.The total population of Dilla is estimated to be 102,624, of these 50,286 are males and 52,338 are females.Livestock production is an essential agricultural sector in Ethiopia, accounting for around 18% of total GDP 24,25 .According to the Central Statistical Agency (CSA) (2021), the Gedeo zone has an estimated livestock population of 133,925 cattle, 197,846 sheep, and 22,621 goats 26 .

Sample size determination.
The sample size was determined using single and double population proportion formulae for three objectives (meat hygiene knowledge, practices, and associated factors among meat handlers).The single population proportion formula yielded the highest sample size for objective one (knowledge about meat hygiene), using the following assumptions: a 95% confidence interval (CI) and a 5% margin of error (d), and by taking a proportion of good level of meat handling knowledge 52.2% from previous study conducted in North Shewa Zone, Oromia, Ethiopia 27 .We used the following correction formula to compute the adjusted sample size (because the total number of meat handlers working at butcher shops in the selected areas was < 10,000), and after adding a 10% non-response rate, the final sample size for this study was 242.
where Ni is the initial sample size, N f is the final sample population.
Sampling procedure.The list of existing butcher shops in Gedeo zone was obtained from the Gedeo Zonal Health Department's Health, Health Related Product Quality Control Authority.According to Health Related Product Quality Control Authority data, there were 179 butcher shops in Gedeo Zone in 2022.In this study, we randomly selected 100 butcher shops from a total of 179 butcher shops.Study participants were proportionately allocated to each selected butcher shops based on the number of meat handlers.Then, the sampling frame was prepared for each selected butcher shops using the updated list of meat handlers.Finally, study participants were selected using a simple random sampling technique from each establishment.
Data collection tools and procedures.Data was collected using a semi-structured, pretested questionnaire.The questionnaires include socio-demographic information, meat handlers' meat hygiene practices and knowledge, as well as factors associated with meat handling knowledge and practices.The questionnaire was adapted from previously published research works 13,21,28,29 , translated to Amharic for ease of understanding during data collection, and then retranslated back to English to ensure consistency.The questionnaires were administered face to face by a trained health professional.
www.nature.com/scientificreports/Measurement of variables.The primary outcome variables were meat hygiene knowledge and practices among meat handlers.We used 14 closed-ended questions with three alternative answers (such as "True," "False," and "Don't know") to assess meat hygiene knowledge and practices among meat handlers.The minimum and maximum score for meat hygiene knowledge and practices were 0 and 14, respectively.Level of meat hygiene knowledge: If the meat handler scored less than 70% on meat hygiene knowledge related questions (answered less than 10 questions out of 14 questions), the meat handler was considered to have a "poor level of meat hygiene knowledge."If a meat handler scored 70% or higher on the meat hygiene knowledge questions (answered 10 questions or more out of 14 questions), the meat handler was considered to have a "good level of meat hygiene knowledge" 14,21,30 .
Level of meat hygiene practices: If the meat handler scored less than the 70% of meat hygiene practice related questions (answered below 10 questions out of 14 questions), the meat handler considered as having a "poor level of meat hygiene practices." If a meat handler scored 70% or higher on the meat hygiene practice-related questions (answered 10 or more questions out of 14), the meat handler was considered to have a "good level of meat hygiene practices" 14,21,30 .

Statistical analyses.
Data were analyzed using Stata version 17.0 (Corporation, College Station, TX: Stata-Corp LLC) and presented using frequencies and percentages.A multivariable binary logistic regression model was used to identify factors associated with meat handlers' food safety knowledge and practice.Adjusted odds ratios (AOR) with 95% confidence intervals (CI) and p-values < 0.05 were used to declare statistically significant associations.Independent variables with p < 0.25 in the univariate analysis were included in the final multivariable logistic regression model.The variance inflation factor (VIF) was used to test multicollinearity; the result was less than 2, and the model fitness was checked using the Hosmer and Lemeshow goodness-of-fit test; the result was 0.72.

Ethical approval and consent to participate.
The study was conducted after receiving ethical clearance from the Institutional Review Board (IRB) of the College of Health Science and Medicine, Dilla University (reference number: 017/2019; and protocol unique number: 002/19-11).We obtained written informed consent from meat handlers.All methods were performed in accordance with the relevant guidelines and regulations.

Results
Socio-demographic characteristics of study participants.This study included all 239 randomly selected study participants, with a 98.8% response rate.The majority of the study participants 149 (62.3%) were male with the mean (± SD) age of 27 (± 5.16) years.The majority of the study participants, 163 (68.2%), had completed primary education, and more than half (52.3%) were married, with a mean monthly income of 2923.01Ethiopian birr.More than half of the study participants (56.1%) had worked for less than 5 years, and the majority of study participants 214 (89.5%) worked more than 8 h per day.However, 91 (38.1%) of participants had received meat hygiene training, and 84 (35.1%) of participants had regular medical checkups (Table 1).

Meat hygiene knowledge among meat handlers.
In this study, almost all of the participants, 228 (95.4%), knew that washing hands reduces the risk of meat contamination.The majority of participants, 195 (81.6%) knew that insect such as cockroach and flies can contaminate row meat, and 207(86.6%) of study participants knew that food contamination is caused by unclean instruments and work surfaces.However, nearly half of the participants (48.1%) are unaware that healthy meat handlers can be carriers of pathogens found in food, 133 (55.6%) study participants didn't know contaminated meat can cause meat borne disease, 97 (40.5%) are unaware that meat handlers with open wounds, gastroenteritis, or diseases of the ear or throat should not handle meat, and three fourth (75.7%) of the study participants didn't knew the ideal temperature for storing fresh meat (Table 2).

Attitude of meat handlers towards meat hygiene.
The majority of the study participants reported that positive attitudes toward storing raw and cooked food separately (89.1%), regular meat safety training (83.4%), covering nose or mouth, during sneezing and coughing (61.4%), food handlers with abrasions or cuts on their hands should not handle food without gloves (69.8%), regular medical examination for the meat handlers (95.8%), wearing personal protective equipment (71.1%), disinfection and clean working surfaces and utensils with safe water (86.7%), and washing hands with soap and water (90.7%).However, 50.2%, 55.2%, and 45.9% of the study participants disagreed that raw meat and raw vegetables should never be cut with the same knife or cutting board, that wearing jewelry like watches, earrings, and rings increases the risk of meat contamination, and that the temperatures of refrigerators and freezers should be checked frequently, respectively (Table 3).

Meat hygiene practice of meat handlers.
The vast majority of study participants practice unsanitary meat handling.More than half of the study participants, 71.5%, 68.2%, and 63.6%, respectively, did not use gloves during work, did not wash their aprons after each day's work, and did not wear a mask during work.In addition, 53.1% of meat handlers did not wear a cap while working, 60.3% use and paint nail polish while handling meat, and 59.8% handled money while processing meat.Furthermore, 51.5% of participants reported that they handled meat when they had an illness and 79.9% of participants handled meat when they had cuts, wounds, bruises, or injuries on your hands.However, the majority of study participants (83.3%) reported washing their Vol:.(1234567890 www.nature.com/scientificreports/hands with water and soap before and after touching raw meat, 95.0% after using the toilet, and 58.2% after smoking, sneezing, or coughing (Table 4).
In this study, the overall mean and standard deviation for food safety attitudes among meat handlers was 0.31 ± 0.462, indicating that 30.1% of meat handlers had positive attitudes toward food safety practices, the overall mean and standard deviation (SD) for meat safety knowledge was 0.62 ± 0.488, indicating that 38.5% of meat handlers had good knowledge toward hygienic meat handling practices, and the overall mean and standard deviation (SD) for hygienic meat handling knowledge was 0.75 ± 0.435, indicating that 25.1% of meat handlers had good knowledge towards meat hygiene practices among meat handlers (Fig. 1).

Factors that associated with meat hygiene knowledge among meat handlers. Variables with
p-values less than 0.25 in bivariable logistic regression analysis, such as gender, age, educational level, job type, work experiences, supervision by health workers, and meat hygiene training, were candidates for multivariable logistic regression analysis.Gender, educational status, supervision by health workers, and meat hygiene training were significantly associated with meat hygiene knowledge among meat handlers with p-values less than 0.05 in multivariable logistic regression analysis.
Workers with a secondary education or higher were 2.26 times more likely to have a good level of meat hygiene knowledge than their counterparts [AOR 2.26, 95% CI: 1.18-4.33,p-value = 0.014], workers who had meat hygiene training were 1.97 times more likely to have a good level of meat hygiene knowledge than workers who did not have meat hygiene training [AOR 1.97, 95% CI: 1.04-3.75,p-value = 0.038], workers who were supervised by a health professional were 3.05 times more likely to have a good level of meat hygiene knowledge than those who were not [AOR 3.05, 95% CI: 1.53-6.07,p-value = 0.002].However, male workers were 0.38 times less likely have a good level of meat hygiene knowledge than females [AOR 0.38, 95% CI: 0.21-0.71,p-value = 0.002] (Table 5).

Factors that associated with meat hygiene practice among meat handlers.
In bivariable logistic regression analysis, variables such as gender, educational status, work experiences, supervision by health workers, meat hygiene knowledge level, regular medical check-up, and meat hygiene training were significantly associated with meat hygiene practices, with a p-value less than 0.25.Finally, in multivariable logistic regression, the independent variables that were significantly associated with meat hygiene practices at a p-value less than 0.05 were educational level, work experiences, supervision by health workers, and meat hygiene training.
Accordingly, those with 5-10 years of work experience are 4.31 times more likely to practice meat hygiene practices than their counterparts [AOR 4.31, 95% CI: 1.29-14.34,p-value = 0.010], those with secondary and higher education are 2.58 times more likely to practice meat hygiene practice than those with primary education

Discussion
The www.nature.com/scientificreports/studies conducted in Amhara National Regional State (11.1%) 19 , Bangladesh (20%) 16 , and Jigjiga Town, Ethiopia (22%) 21 among meat handlers.However, a study in Malaysia found that 61.7% of abattoir employees had a good knowledge of food safety 9 , and a study in Lagos State, Nigeria found that 71.1% of meat handlers had a good knowledge 17 , both of which were higher than the result of this study.This study finding is consistent with a study conducted in Amhara National Regional State, which found that 25.7% of butcher shop and abattoir workers practiced good food safety practices 19 , as well as a study conducted in slaughter houses in north-central Nigeria (25.5%) 18 .However, the finding of this study is higher than those of a studies conducted in Jigjiga Town, Ethiopia (1.1%) 21 , Bangladesh (16.3%) 16 , and in Bishoftu City, Ethiopia (16.3%) 20 .On the contrary, this study finding is lower than those of studies conducted among butcher shops in Gondar town (66.4%) 14 , meat handlers in North Shewa Zone, Oromia, Ethiopia (51.3%) 27 , among meat handlers in Lagos State, Nigeria (66.6%) 17 , and abattoir workers in Malaysia (77.7%) 9 .The discrepancy in food safety practice level could be attributed to differences in the study tool employed, the period of the study, and variations in the study population's socio-demographic and economic status.
Gender, educational status, health worker supervision, and food safety training were found to be significantly correlated with meat handlers' meat hygiene knowledge.In this study, the male gender was found to be Table 6.Factors associated with the meat hygiene practice among meat handlers, in Gedeo zone, Southern Ethiopia, 2022 (n = 239).Significant values are in bold.and Bangladeshi 33 , which found that male food handlers have a higher level of meat hygiene knowledge than females.Variations in results could be explained by differences in the study population, sample size, study settings, and socio-cultural status of study participants.

Gender
According to the findings of this study, secondary or higher education is a predictor of maximizing meat hygiene knowledge.Meat handlers with a secondary education or higher were 2.26 times more likely than their counterparts to have a good level of meat hygiene knowledge in this study.This study finding is in agreement with those of studies conducted in Chitwan, Nepal 29 , in Amathole District, Eastern Cape Province, South Africa 34 , and in Bangladesh 16 .The possible explanation is that education increased workers' awareness of food safety practices in the workplace while also increasing their exposure to mass media and other information sources.
According to the findings of this study, the majority of meat handlers (61.9%) had not received meat hygiene training.This study's findings are comparable to those of studies conducted in two Bangladeshi districts (85%) 35 , in Gondar Town, Ethiopia (66.04%) 36 , and in Mekelle City, Ethiopia (61.5%) 37 .
According to the literature, food handler training in food hygiene is important for providing safe food to consumers 38 , and handling food without food hygiene training increases the risk of cross contamination 29 .According to the current study, meat handlers who received meat hygiene practices training were 1.97 times more likely to have higher meat hygiene knowledge than those untrained ones.This study finding is supported by study conducted in Bangladesh 16 , in Chitwan, Nepal 29 .Study finding from abroad reported that training can improve knowledge of employee 39 that is why in this study meat handlers who received meat hygiene training have higher meat hygiene knowledge.Furthermore, a study conducted in Malaysia revealed that food hygiene training improves food handlers' food safety knowledge and practice 40 .This could be due to food handlers who did receive food hygiene training having the necessary knowledge and experience as a result of professional advice they received from trainees during training.Furthermore, training may have an effect on changing the behaviors of food handlers to adhere to food safety practices.
In this study, regular supervision by a health professional was found to be significantly associated with meat hygiene knowledge (p = 0.002).Meat handlers who were supervised by health workers were 3.05 times more likely to be familiar with meat hygiene practices than those who were not.This study's findings are supported by a study conducted among food service workers in Bangladeshi hospitals 33 .The possible explanation is that during supervision, health workers and managers may provide on-the-job training on food safety practices, which is why workers supervised by health workers or managers have good knowledge of meat hygiene practices.
In this study educational status, supervision by health care workers, work experiences and meat hygiene training were factors significantly associated with meat hygiene practice among meat handlers.Meat handlers with educational status of secondary and above were 2.58 times more likely practice meat hygiene practices than the counterparts.This study finding is supported with study conducted in North Shewa Zone, Oromia, Ethiopia 27 , study conducted in Kenya 41 , study conducted among meat-handlers in Metropolitan City of Kathmandu, Nepal 11 .The probable explanation is that those with a higher level of education can understand some of the regulations and instructions pertaining to meat hygiene practices.
In this study, supervision by a health professional was found to be significantly associated with meat hygiene practice (p = 0.000).Meat handlers who were supervision by health professionals were 4.01 times more likely to be familiar with meat hygiene practices than those who were not.This study finding is inline by studies conducted among food handlers in Arba Minch 42 , in Gondar city 43 and among meat handlers in Lagos State, Nigeria 17 .The possible explanation might by during supervision by health works give awareness creation training for meat handlers by giving practical support and feedback for meat handlers.
Meat handlers with 5-10 years of experience were 14.31 times more likely than workers with less than 5 years of experience to practice meat hygiene practices.This study finding is supported by studies conducted in Lagos State, Nigeria 17 , in north-central Nigeria 44 , in North Shewa Zone, Oromia, Ethiopia 27 and study conducted in Food Handlers of Fiche Town, North Shewa Zone, Ethiopia 45 which found that experienced workers had good practice towards meat hygiene practices.The possible explanation could be that experienced food handlers may have better knowledge and skills regarding meat hygiene practice.
In this study, 38.1% of meat handlers were trained in meat hygiene practices, which was higher than studies conducted in North Shewa Zone, Oromia, Ethiopia among Meat Handlers (12.1%) 27 , in Nepal among meat handlers (7%) 11 , in Dharan Municipality of Eastern Nepal (30.4%) 46 , in the Dhaka megacity of Bangladesh among red meat handlers (21.25%) 47 .
In this study, meat handlers who received meat hygiene training were 2.20 times more likely to practice meat hygiene than those who did not receive meat hygiene training.This study's findings are supported by studies conducted in the Gamo Gofa Zone of Southern Ethiopia 42 , Bishoftu City, Ethiopia among meat handlers 20 , in Kenya 41 , in north-central Nigeria 44 , in Gondar city 43 , in Bangladesh among meat handlers 16 , and in eastern Cape Province, South Africa among slaughter house workers 34 .A possible explanation is that meat handlers become aware of proper meat hygiene practices as a result of meat hygiene training.
These findings suggest that good meat hygiene knowledge and practices could serve as an important component in promoting proper meat handling practices.As a result, more emphasis should be given to educating meat handlers, providing training to meat handlers working in butcher shops, and conducting frequent supportive supervision by health professionals, as well as considering these aspects and developing policy and regulations to guide and monitor butcher shops across the country in order to protect communities from potential meatborne disease. https://doi.org/10.1038/s41598-023-42225-8

Table 3 .
Attitude towards meat hygiene practices among meat handlers in Gedeo Zone, Southern Ethiopia, 2022 (n = 239).Raw vegetables and raw meat should never be cut on the same cutting board or with the same knife

Table 4 .
Meat Meat hygiene knowledge, attitudes, and practices of meat handlers.