Bioactive peptides have been studied in several sources due to their valuable potential in the pharmaceutical and food industries. Abalone viscera, which are normally discarded as byproducts, are a rich source of protein. Thus, the aim of this study was to explore the potential bioactivity of peptides derived from abalone viscera (Haliotis fulgens and Haliotis corrugata) after hydrolysis with a commercial mixture of enzymes. The hydrolysates obtained were fractionated using gel filtration chromatography. The resulting hydrolysate fractions were investigated for their antimicrobial and cytotoxic activities, including the expression of gelatinases mmp-2 and mmp-9 in human prostate cancer cell lines (PC3). Results showed antimicrobial activity for protein fractions of H. corrugata against Proteus mirabilis and Pseudomona aeuroginosa (66.2–116.25 kDa), Bacillus subtilis (6.5–21.5 kDa), and Aspergillus niger (97.4–116.25 kDa), while H. fulgens peptide fractions (200–31 kDa) displayed activity against six bacterial strains, and fractions from 116.25 to 21.5 kDa had effects on the fungus A. niger, Alternaria alternata, and Aspergillus flavus. Additionally, protein fractions displayed cytotoxic activity, inhibiting 30.4–53.8% of PC3 cellular growth. Selected fractions decreased the PMA-induced and not-induced expressions of mmp-2 and mmp-9 in PC3 cells. Abalone viscera, as byproducts, can be used as a potential source of antimicrobial and anticancer peptides.
Marine organisms represent an important source of bioactive compounds due to their development in a wide range of environments, which requires adaptation mechanisms. These mechanisms include the synthesis of bioactive molecules that function as signaling molecules, defense against predators, elimination of competitors, and inhibition of bacterial and/or fungal invasion1. Additionally, these compounds are synthesized in high concentrations to allow for their excretion into the aqueous medium and to ensure their efficacy. Currently, over 3,000 compounds (e.g., proteins, polysaccharides, pigments, etc.) with novel bioactivity have been described from marine sources2.
The main sources of marine bioactive compounds are bacteria, cyanobacteria, algae, sponges, cnidaria, bryozoans, mollusks, tunicates, echinoderms, and marine sneaks1. However, only a few of them have been characterized and commercialized. Examples of these include ziconotide and brentuximab vedotin, which are marine peptides derived from mollusks Conus magus and Dolabella Auricularia, respectively. These peptides were approved by the Food and Drug Administration (FDA) in 20113,4,5. Trabectedin, isolated from a sea squirt (Ecteinascidia turbinate), was approved for use against sarcoma by the European Union (EU) in 2007 and by the FDA in 20156. Eribulin mesylate, derived from a marine sponge (Halichondria okadai), received FDA approval for use against breast cancer in 2010 and EU approval in 20117.
Additionally, a wide variety of marine compounds have been used for diagnostic and experimental purposes. For instance, green fluorescent protein (GFP) isolated from the jellyfish Aequorea victoria is used for molecular labeling8. Limulus amebocyte lysate (LAL), derived from the crab Limulus polyphemus, is utilized for detecting lipopolysaccharides from gram-negative bacteria9. Hemocyanin KLH, obtained from the marine mollusk Megathura crenulata, serves as a vaccine carrier for treating bladder carcinoma10. Furthermore, compounds like tetrodotoxin, okadaic acid, and palitoxin are toxins used in the study of ion channels in eukaryotic cells. Additionally, there are 20 other candidates in phase I, I/II, II or III clinical trials1. Therefore, marine organisms offer promising prospects for the discovery of new bioactive molecules that could be valuable in the nutraceutical industry, pharmacology, and research.
The abalone (Haliotis spp.) fishery is highly valued as a seafood delicacy in many parts of the world, especially in Asia. Thus, the global production of farmed abalone has significantly increased in recent years11. However, during the abalone processing, the viscera, which account for 15–25% of their wet weight, are often discarded12. This results in the annual disposal of tons (t) of viscera. For instance, in Mexico alone, approximately 42.9–71.5 t of viscera are generated from an annual fishery production of 286 t13. Therefore, there is potential for the valorization of abalone viscera byproducts, as they are a rich source of protein and can be used as precursors of bioactive peptides14. There is substantial evidence of bioactive compounds in abalone, such as peptides and polysaccharides, that exhibit various bioactivities, including nutraceutical properties15 and pharmacological properties16,17,18,19,20,21,22,23,24,25,26,27.
Given the valuable properties of bioactive peptides, it is hypothesized that peptides derived from hydrolyzed abalone viscera could exhibit antimicrobial activity against various pathogens and cytotoxic effects against cancer cells. The hypothesis is based on previous research that has demonstrated the presence and bioactivity of bioactive compounds, such as peptides, in mollusk viscera. For example, mollusk visceral extracts have demonstrated effectiveness against Escherichia coli K1, Pseudomona aeruginosa, Acinetobacter baumanii (Perna viridis28, as well as Vibrio harvey (Anadara granosa29). Furthermore, the administration of abalone viscera to mice with breast cancer has shown significant inhibition in tumor progression by suppressing epidermal growth factor (EGF), vascular endothelial growth factor (VEGF), cyclooxygenase-2 (Cox-2), and fibroblast growth factor (FGF)23. Some of these factors are regulated by matrix metalloproteinases (MMPs, also known as gelatinases), which are zinc-dependent endopeptidases involved in a variety of pathological processes such as tumor invasion and metastasis30. Gelatinases (MMP-2, MMP-9) play an important role in cancer invasion and metastasis and have been detected in human fibrosarcoma cell line HT1080 and PC-3 cells31,32. A high level of mmp-2 and mmp-9 gene expression has been linked to an unfavorable prognosis in human cancer cell lines, including prostatic adenocarcinoma33. Thus, mmp-2 and mmp-9 gene expression works as indicators of a favorable prognosis in patients with some types of cancer.
The increase in bacterial resistance, resulting from the widespread use of antimicrobials for disease control, and the rising cases of cancer diseases have created and urgent need for the discovery of novel antimicrobial and anticancer molecules to combat drug resistance and cancer.
Therefore, the aim of this study is to investigate the antimicrobial activity of visceral extracts from the two commercial produced abalone species in Mexico (H. fulgens and H. corrugata) against 11 opportunistic pathogens from the bacterial family Enterobacteriacea, which are responsible for a wide range of infections. Additionally, four common fungal species are included in the evaluation. Moreover, the anti-cancer activity of visceral extracts was evaluated using a grade IV prostatic adenocarcinoma PC-3 (ATCC CRL-1435™) cell line, which represent the second most commonly occurring cancer in men.
Materials and methods
Abalone viscera from blue (Haliotis fulgens) and green abalone (Haliotis corrugata) were donated by the Sociedad Cooperativa Punta Abreojos, S.C. of C.V. in northern Baja California Sur, Mexico.
The cell line, grade IV prostatic adenocarcinoma PC-3, was obtained from the American Type Culture Collection (ATCC CRL-1435) by the Centro de Investigacion y Asistencia en Tecnologia y Diseño del Estado de Jalisco (CIATEJ).
Protein extract and enzymatic digestion
Three batches of viscera, weighing 150 g each, were used from blue and green abalone, respectively. The protein from the viscera was extracted following the method described by Nguyen31 with some modifications. In brief, digestive organs were homogenized using a blender, and the pH was adjusted to 5.634 using a 150 mM citric acid-sodium citrate buffer, reaching a final volume of 500 mL. An aliquot, 1 mL of each extract was collected for further analysis. Enzymatic hydrolysis of the crude extract was performed using Wobenzym (Societe des Produits Nestle S.A. Vevey Switzerland) (Supplementary Table I). Prior to this, enzyme pills were pulverized and added to the crude extract. Enzymatic digestions were carried out using 0.4, 0.6, 0.8, 1, 10, 12.5, and 15 g of pulverized Wobenzym pill. The mixture was then incubated at 37 °C in a water bath for 4 and 24 h, respectively, with constant agitation. Subsequently, the enzymatic hydrolysates were centrifuged at 10,000 g, − 4 °C for 20 min (Beckman, Mod. Js-HS). The soluble fraction was collected and further centrifuged at 100,000 g, − 4 °C for 60 min (Beckman, Mod. L7 Ultracentrifuge). The resulting soluble fraction was collected and filtered using sterile Whatman 541 paper (VWR) to remove lipids and non-hydrolyzed molecules. The hydrolyzed extracts were evaluated using SDS-PAGE electrophoresis.
Protein quantification of the visceral and hydrolyzed extracts, as well as the chromatographic fractions, was performed using the methods described by Bradford35 and Lowry36. This was done using a spectrophotometer SmartSpec 3000 (Bio-Rad, Hercules, CA, USA), and a microplate reader (Bio-Rad Benchmark). Bovine serum albumin was used as standard.
Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE)
Protein separation by SDS-PAGE was performed following the method described by Laemmli37 using a Mini Protean II system (Bio-Rad, 2000, CA, USA). Samples were mixed with 4 × sample buffer containing 50 mM Tris–HCl pH 6.8, 20% glycerol, 10% SDS, 10% β-mercaptoethanol and 0.05% bromophenol blue. The samples were then boiled for 10 min and loaded into either 10% or 15% polyacrylamide gel. A broad-range molecular weight standard (Bio-Rad, 1610317, CA, USA) was also loaded into the gel. Electrophoresis was carried out at 90-V and room temperature using a vertical electrophoresis unit (Bio-Rad Protean II, CA, USA). After electrophoresis, the gels were stained with Coomassie blue solution (0.05% (w/v) Coomassie Brilliant Blue R-250, 7% v/v acetic acid, 40% v/v methanol) for 2 h. Protein bands were then visualized by soaking the gels in a distaining solution (7% v/v acetic acid and 40% v/v methanol) for 2 h. Gel images were digitalized using a gel imager (Chemi Doc XRS, Bio-Rad, CA, USA). The relative molecular mass of protein bands was determined by comparing their migration with the molecular weight standard.
Protein fractionation by gel filtration chromatography
Proteins obtained from enzymatic hydrolysis, 2 mL each from green and blue abalone, were subjected to high-resolution gel filtration chromatography using a Sephacryl S-100 column (Cytiva Lifesciences, USA) with dimensions of 1.0 × 31 cm. Prior to sample loading, the column was pre-equilibrated with 150 mM citric-citrate sodium buffer (pH 5.6). Protein fractions were eluted from the column using the same buffer at a flow rate of 0.8 mL/min. A total of fifty-three fractions, each with a volume of 2.4 mL, were collected. Protein peak fractions were determined based on their absorbance at 280 nm. All samples were preserved at − 4 °C until further use.
Antimicrobial activities of the visceral extracts and chromatographic protein fractions were assessed using a radial diffusion method in agar, following the protocols described by Fothergill38 and Valgas39. The bacteria and the filamentous fungi used in the experiments are listed in Table 1. Petri dishes containing tryptic soy agar (TSA) and potato dextrose broth (PD) medium were inoculated with 100 µL of bacteria (1 × 108 cell/mL) and fungi (0.4–5.0 × 104 spore/mL), respectively. Afterward, paper filter disks (Whatman, 1/4, VWR) were placed in the Petri dishes, and 20 µL of hydrolyzed visceral extracts or chromatographic protein fractions were added to their respective filter disks. The agar plates were then incubated at 37 °C, and the growth of bacteria was monitored after 72 h, while fungal growth was monitored after 48 h. The presence of antimicrobial activity was determined by measuring the diameter of the zone of inhibition that appeared around the filter disk following the incubation period. Positive controls for bacteria included gentamicin (0.1 mg/mL), kanamycin (0.1 mg/mL), penicillin (20 U/mL), and streptomycin/penicillin (20 U/mL). For fungi, positive controls consisted of amphotericin (0.4, 4.0, 25.0, 33.0, 50.0, and 100 mg/mL), ketoconazole (0.4, 4.0, 25.0, 33.0, 50.0, and 100 mg/mL), and fluconazole (3 and 15 mg/mL). As a negative control, citric-citrate sodium buffer (150 mM, pH 5.6) was used.
The chromatographic fractions, 1.5 mL each, were lyophilized. Prostate cancer cells PC-3 (CRL-1435) were cultured in F12K medium (Gibco Life Technologies, Eggenstein, Germany), supplemented with 10% fetal bovine serum (FBS, Gibco), and antibiotics (1% penicillin/streptomycin, Gibco Life Technologies, Eggenstein, Germany)31,40. The cells were maintained in a humid atmosphere with 5% CO2 at 37 °C and passaged serially when the cell density reached 8 × 106 cells per box. For instance, PC-3 cells become confluent after 4 days, and the number of cells obtained from one T-75 flask was 8 × 106 cells. On the 4th day, the cells were trypsinized from the flask and plated in wells for the assay.
PC3 cells (1 × 104 cells/well) were seeded into 96-well plates. Briefly, PC3 cells were detached from the box using 0.25% trypsin (Gibco Life Technologies, Eggenstein, Germany) by a 5 min incubation at 37 °C. Then, trypsin was inactivated by adding 5 mL of fresh medium. The cells suspension was recovered after centrifugation at 1500 rpm for 10 min. The supernatant was discarded, and the pellet (cells) was re-suspended in 1 mL of medium. The number of cells was estimated using a Neubauer chamber. Then, the PC3 cells were seeded into a 96-well plate and incubated for 24 h at 37 °C to allow cell adhesion to the plate. Afterwards, the medium from each well was discarded, and 100 µL of protein fractions were added. The protein concentration used was according to Nguyen31. Two hundred and 400 µg/mL were added to each well, respectively, followed by a 24 h incubation at 37 °C. Subsequently, 20 µL MTT reagent (5 mg/mL) was added into each well, and the plate was further incubated for 24 h at 37 °C. Finally, 100 µL of DMSO was added to the medium to dissolve the formazan crystals, and the mixture was incubated for 15 min at 37 °C. The formazan was then measured at a wavelength of 595 nm. The plates were read on a microplate reader (Bio-Rad, Bechnmark) before the data were analyzed. The survival rate was calculated as follows: survival rate (%) = 100 × (OD 595 value of tested sample)/OD 595 value of untreated cells).
MMP-2 and MMP-9 expression
The expression of gelatinases mmp-2 and mmp-9 was measured according to Van-Ta40 and Nguyen31. Cultured PC3 cells were seeded into 48-well plates at a density of 2 × 105 cells/well. The cells were cultured in F12K without FBS for 24 h at 37 °C and 5% humidity. Afterwards, the medium was removed, and chromatographic fractions (300 µg/mL) from H. fulgens were added and incubated for one hour, followed by incubation with PMA (10 ng/mL) for 36 h. After incubation, the culture medium was removed, and cells were lysed on the plate using RLT (RNeasy Mini Kit, QIAGEN, Cat No. 74104) for RNA extraction. Samples were collected and stored at − 80 °C for further total RNA extraction.
RNA extraction and droplet digital PCR (ddPCR)
Total RNA was isolated from the PC3 cells using RNeasy Mini Kit (QIAGEN, Cat No. 74104), following the manufacturer´s instructions. The concentration and purity of the RNA were determined by spectrophotometry using a NanoDrop ND-2000, measuring absorbance at 260/280 and 260/230 nm absorbance. The RNA integrity was assessed by running it on a 1% (w/v) agarose/synergel agarose gel. To ensure the absence of DNA contamination, a direct PCR was performed using 1 µL (50 ng/µL) of each RNA preparation with 28S ribosomal specific primer, a non-amplified control. Subsequently, 8 ng of total RNA was used from each verified RNA sample for cDNA synthesis using the ImProm-II Reverse Transcription System (Promega, Madison WI) and oligo-dT primer, following the manufacturer´s instructions.
The expression of mmp-2 and mmp-9 was determined using the droplet digital PCR System (QX200 Droplet Digital PCR System fromBio-Rad). Briefly, each 20 µL reactions consisted of 1X EvaGreen ddPCR Supermix (QX200 ddPCR EvaGreen Supermix, Cat. No. 1864033, Bio-Rad), 100 nM gene-specific primers (Table 2), and 1 µL of the cDNA sample (100 ng). The reaction mixture was combined with 70 µL of Droplet Generation Oil (Bio-Rad), partitioned into 14,000–17,000 droplets in QX200 Droplet Generator (Bio-Rad), transferred to 96-well plates, and sealed. PCR amplification was carried out in a C1000 Touch Thermal Cycler (Bio-Rad) under the following cycling conditions: 1 cycle at 95 °C for 5 min, 40 cycles at 95 °C for 30 s, 60 °C for 30 s, 72 °C for 30 s, 1 cycle at 4 °C for 5 min, 90 °C for 5 min with 2 °C/s ramp rate. Immediately after the end-point amplification, the fluorescence intensity of individual droplets was measured using the QX200 Droplet Reader (Bio-Rad). Data analysis was performed using the QuantaSoft droplet reader software (Bio-Rad). The absolute transcript level was calculated in copies/µL PCR.
Statistical analyses were performed using the GraphPad Prism Software (San Diego, CA, USA). The results are expressed as average ± s.d. Significant differences (p < 0.01) were determined with a one tailed students t-test performed in a pair wise manner.
Optimization of hydrolysis parameters for abalone viscera
Protein viscera from abalones (H. fulgens and H. corrugata) were hydrolyzed using Wobenzym (Societe des Produits Nestle S.A. Vevey Switzerland). Optimization parameters for protein extraction were performed using viscera from H. fulgens due to the limited number of samples collected for H. corrugata. This is because the abalone fishery, mainly supported by Haliotis fulgens and H. corrugata, is currently experiencing a decline. The optimization process involved two different centrifugation conditions: 10,000 × g at − 4 °C for 20 min and 100,000 × g at − 4 °C for 60 min. Furthermore, the incubation time of hydrolysis (2, 3, and 4 h) and enzyme concentration (ranging from 0.4 to 1 g/mL) after 4 h of hydrolysis were evaluated (Fig. 1).
No difference was observed in protein recovery when using two different centrifugation conditions (Fig. 1A). Thus, a centrifugation condition of 10,000 × g was used for all the subsequent protein recovery extractions. Hydrolysis of the viscera using 2 g of Wobenzym (Societe des Produits Nestle S.A. Vevey Switzerland) for 4 h incubation resulted in a reduction in protein concentration and increased protein hydrolysis, as indicated by SDS-PAGE analysis (Fig. 1C). Increasing the amount of Wobenzym used for visceral hydrolysis led to a higher degree of molecular protein hydrolysis; however, protein bands remained within a wide range of 45–200 kDa (Fig. 1D). To enhance visceral protein hydrolysis, Wobenzyme amounts of 10, 12.5, and 15 g were used for hydrolysis at 37 °C for 24 h. Elevated protein hydrolysis was observed with 10 and 15 g of Wobenzym (Table 3). Nonetheless, no differences were observed in the protein pattern band patterns on SDS-PAGE (Supplementary Fig. 1).
Protein fractionation by gel filtration
The hydrolyzed samples with Wobenzym (Societe des Produits Nestle S.A. Vevey Switzerland) shown in Table 1 were used for protein fractionation through gel filtration. Each hydrolyzed viscera, containing 36 mg of total protein, underwent fractionation. A total of 53 fractions were collected. Three protein peaks were observed at the absorption of 280 nm, representing high molecular weight (116.25–66.2 kDa), medium molecular weight (45–21.5 kDa), and low molecular weight (21.5–6.5 kDa) proteins (Fig. 2). The fractions corresponding to each peak were pooled and used for antimicrobial and cytotoxic activity assays.
Antimicrobial and antifungical activity
Fractions obtained from the hydrolyzed viscera of abalones were tested against nine Gram-negative and two Gram-positive bacteria, as outlined in Table 4 and Supplementary Table II. The concentration used for antimicrobial and antifungical activity for H. corrugata was 12 mg/mL due to the lack of viscera sample obtained for the peptide generation. The results showed that the abalone fractions exhibited activity against five Gram-negative and two Gram-positive bacteria (Fig. 3). However, no antimicrobial activity was observed against S. sonnei, S. flexneri, S. thyphimurium, and S. enteritidis. Fractions derived from H. corrugata containing proteins ranging from ~ 66.2 to 116.25 kDa displayed antimicrobial activity against P. mirabilis and P. aeruginosa, while low molecular weight proteins (6.2–21.5 kDa) demonstrated sensitivity only against B. subtilis. Fractions obtained from H. fulgens showed potent activity against five Gram-negative bacteria (Proteus mirabilis, P. aeruginosa, Enterobacter aerogenes, E. coli and Salmonella thyphi) and two Gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus).
Antifungal activity was observed in both H. corrugata and H. fulgens protein fractions (Table 5, Figs. 4 and 5). Fractions derived from H. corrugata, containing protein peptides ranging from ~ 97.4–116.25 kDa, displayed inhibition specifically against A. niger. On the other hand, fractions from H. fulgens displayed inhibition against Alternaria alternata, Aspergillus niger, and Aspergillus flavus using fractions containing low molecular weight proteins (~ 31–66.2 kDa). None of the tested fractions for each species demonstrated antifungal activity against Mucor sp.
The cytotoxic effect of visceral fractions obtained from abalones was investigated on the PC-3 cell line using the MTT assay at concentrations of 200 and 400 µg/mL as shown in Fig. 6. Fractions containing peptides of medium molecular weight (61.2–31 kDa) displayed cytotoxic activity resulting in a 50% inhibition of cell. Fractions 33, 34, and 38–41 displayed reduced cell survival ranging from 30–54% after treatment with 400 µg/mL.
Expression of mmp-2 and mmp-9 in PC-3 cell lines
The expression of mmp-2 and mmp-9 was tested in PC-3 cell lines, both stimulated and non-stimulated with PMA, after incubation with molecular weight proteins (66.2–31 kDa) derived from hydrolyzed visceral extracts from H. fulgens at concentration of 200 and 300 µg/mL (Fig. 7, Table 6). In PC-3 cell lines stimulated with PMA and treated with visceral fractions (33, 38, and 41), a low expression of mmp-2 and mmp-9 was observed compared to the negative and positive controls. Significant differences were observed for mmp-9 expression with fraction 41 in contrast to both the positive and negative controls (p = 0.010 and p = 0.0005, respectively). However, no difference was observed in the mmp-2 transcript.
Unstimulated PMA PC-3 cell lines with fractions 33 and 41 (300 and 200 µg/mL) displayed downregulation of both gelatinases in contrast to negative controls, with significant differences for mmp-9 transcript (p = 0.05). Fraction 33 with 300 µg/mL led to a 90.3% reduction of mmp-2 and 71% for mmp-9 (p = 0.007). Fraction 41 with 200 µg/mL displayed a reduction of 91.7% and 84.4% for mmp-2 and mmp-9, respectively.
Bioactive peptides can be involved in diverse biological functions depending on their amino acid composition and molecular size. During gastrointestinal digestion, proteolytic enzymes can release bioactive peptides with potential physiological benefits20. In vitro gastrointestinal digestion of marine organisms produces larger quantities of bioactive peptides that are as potent as natural products31. Various strategies have been employed to obtain bioactive peptides in vitro, including exposure to heat, acids, proteolytic enzymes, and more recently subcritical water extracts18. Peptides released by enzymatic hydrolysis represent a high-cost option but provide greater precision in controlling the degree of protein hydrolysis41. Food-grade proteolytic preparations, such as Alcalase 2.4L, Flavourzyme 500L/1000L, Corolase PP, and Promod 144MG, have been widely used for protein hydrolysates42,43,44. However, differences in specificity among proteolytic preparations can lead to variation in the production of low molecular weight peptides45, which also dependent on the protein source being hydrolyzed. In this study, treatment with Wobenzym (Societe des Produits Nestle S.A. Vevey Switzerland) resulted in significant changes in the SDS-PAGE profile, reflecting extensive hydrolysis of medium molecular weight proteins (~ 31–60 kDa) from abalone viscera. However, it appears that Wobenzym does not have sufficient specificity to completely hydrolyze the high molecular weight proteins (> 66 kDa), despite containing a diverse range of cysteine and serine proteases, such as bromelain, papain, trypsin, and chymotrypsin enzymes.
Protein hydrolysates are a valuable source of peptides and amino acids with bioactive properties due to their physicochemical characteristics46. The bioactivity of peptides mainly depends on their structure, molecular mass, and the physicochemical characteristics of the amino acids within the sequence. The hydrolysate obtained from abalone viscera yielded peptides with high molecular weight (~ 200–116.25 kDa), medium molecular weight (~ 116.25–66.25 kDa), and low molecular weight (~ 66.2–31.0 kDa) molecular weight peptides. Medium and low molecular weight peptides have been related with antimicrobial activity in various marine species, including the oyster Crassostrea gigas47, marine snail Cenchritis muricatus48, and mussel species Perna viridis and P. perna49. Aqueous extracts from marine mollusks have shown inhibitory activity against Gram-positive and Gram-negative strains, such as Klebsiella pneumoniae and Salmonella typhi (Melo melo50, Vibrio parahaemolyticus and, Staphylococcus aureus and Candida albicans (Babilonia spyrata51), E. coli, S. aureus, B. subtilis, S. pneumonia, and P. aeruginosa, (G. paradoxa52). In this study, extracts of H. fulgens exhibited a notably wider spectrum of antibacterial inhibition compared to H. corrugata. Low molecular weight proteins from H. fulgens displayed inhibition against P. mirabilis, P. aeruginosa, S. thyphimurium, S. thyphi, E. aerogenes, E. coli, S. aureus, and B. subtilis, while peptides from H. corrugata in the range of 21.5–6.5 kDa were only effective against B. subtilis. High molecular weight peptides (116.25–66.2 kDa) from H. corrugata showed inhibtion solely against P. mirabilis, whereas high molecular weight peptides (200–116.25 kDa) from H. fulgens exhibited inhibition against E. coli. Interestingly, none of the abalone extracts demonstrated inhibitory effects on Gram-negative bacteria such as S. sonnei, S. flexneri, S. thyphimurium, and S. enteritidis.
In contrast to the antibacterial activity observed in the abalone extracts, the antifungal activity was restricted to Alternaria alternate, Aspergillus nigel, and A. flavus using medium molecular weight peptides (66.2–31 kDa) from H. fulgens, and A. niger using high molecular weight peptides (97.4–116.25 kDa) from H. corrugata. Similar antifungal activity has been reported in M. melo against T. mentagarophytes and A. niger50, Patella rustica against Candida albicans52, Cenchritis muricatus against Botrytis cinerea, Fusaryum oxysporum, Aspergillus niger, Candida albicans and, Cryptococcus neoformans53 and, B. spirata against C. albicans51. Despite previous investigations, the antifungal activities of tissue extract from mollusks including gastropods, are limited. The results of this study provide evidence of the potential of visceral extracts as antimicrobial agents.
Antimicrobial peptides exert their antibacterial activity through various mechanisms. The main mechanism involves the attraction, attachment, insertion, and orientation of peptides54. Initially, it was believed that the bactericidal mechanism of positively charged antimicrobial peptides relied solely on their interaction with negatively charged phospholipids in bacterial cell membranes. However, recent studies have revealed that antimicrobial peptides can directly eliminate pathogens by binding to targets outside the cell membrane, such as cell walls or intracellular components55.
A common characteristic of antimicrobial peptides' antibacterial effects is their requirement to come into contact or interact with the bacterial cell surface before exerting their action. Once bound to bacteria, the peptides primarily rely on insertion into the bacterial cell membrane to execute their bactericidal effect. This insertion disrupts the integrity of the cell membrane, leading to intracellular material leakage and disruption of the transmembrane potential. Consequently, this causes abnormal physiological processes and ultimately leads to cell death56,57. The low inhibition presented by abalone peptides on Gram and positive bacteria, compared to positive controls in this study suggest that abalone peptides possess low electrostatic attraction for membrane interaction or hydrophobic properties, allowing a limited insertion of the peptide into the membrane, possibly due to the production of large molecular weight proteins produced in vitro. Both electrostatic and hydrophobic interactions are known to be essential for antimicrobial activity28. However, this could be also an effect of the low concentration of the peptide responsible for antimicrobial activity in the fractions tested. Thus, further investigations are necessary to purify these active compounds and elucidate their identity, chemical composition, and mode of action as antimicrobial peptides.
Cancer is a chronic disease that results in abnormal cell growth in body tissues, leading to destruction of normal cells. The increasing worldwide cases of cancer-related deaths has prompted researchers to search for potential anticancer drugs. Extracts from marine mollusk have exhibited promising cytotoxic activity against cancer cells lines. For instance, the blue copper oxygenated respiratory protein derived from the hemolymph of the Rapana thomasiana demonstrated significant inhibition of cervical cancer cells (59%) and ovarian cell lines (44%), comparable to Tamoxifen, a commercially available anticancer drug58. Moreover, the cyclodepsipeptide Kulokekahilide-2 displayed significant cytotoxic activity against A549, K562, and MCF7 cell lines. It has been demonstrated that the cyclization of the depsipeptide contributes to its cytotoxic properties59.
In this study, cytotoxic activity of chromatographic fractions (33–41) from abalone was evaluated against prostate cancer (PC-3) cell line using two different concentrations. The fractions containing 200 µg/mL showed the highest cell survival rate, except for fractions 37, which exhibited 80% cell survival at both concentrations (200 and 400 µg/mL). This significant cytotoxic activity was accompanied by a reduction of matrix metalloproteinase (MMP) expression, mmp-2 and mmp-9, in the PC-3 cell line, even when stimulated with PMA. MMP proteins are recognized as biomarkers for various diseases, with mmp-9 being overexpressed in several types of tumors (carcinoma, breast, ovarian and cervical cancer, among others)60,61. MMPs play a crucial role in degrading the extracellular matrix (ECM), which consists of collagen, enzymes, and proteins that inhibit tumor cell metastatiss62. Similar results have been reported for the treatment of mice with visceral extract from the abalone Haliotis discus hannai, resulting in a significant inhibition of tumor metastasis through the regulation of Cox-2, EGF, VEGF, and FGF expression levels23. Cytotoxic activity against HT-29 cells has also been observed in the visceral extracts obtained from the same abalone species using different solvents63. More recently, the isolated peptide KVEPQDPSEW (AATP) has shown the ability to suppress the gene and protein expression of MMP2 and MMP9 in the HT1080 cell line by blocking MAPKs and NF-κB pathways64,65. These findings suggest that peptides derived from abalone, including those from H. fulgens and H. corrugata, possess significant antimicrobial, cytotoxic and anticancer activities making them potentially valuable for pharmacological purposes. However, further studies are required to isolate the responsible peptides for these activities and to elucidate their mechanisms of action, which can be tested in clinical trials.
From the previous observations, antibacterial and cytotoxic activities accompanied by decrease in mmp-2 and mmp-9 gene expression suggest that peptides obtained from hydrolyzed abalone viscera possess novel bioactivity against Gram-positive and negative bacteria but limited to few fungal strains. Moreover, peptide fractions contain important regulators of mmp-2 and mmp-9 gene expression, reducing the cytotoxic effect on PC3 cell lines. However, further research is necessary to identify and characterize the molecule responsible for these bioactivities.
The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
Lindequist, U. Marine-derived pharmaceuticals—Challenges and opportunities. Biomol. Ther. 24, 561–571 (2016).
Gerwick, W. H. & Moore, B. S. Lessons from the past and charting the future of marine natural products drug disscovery and chemical biology. Chem. Biol. 19, 85–98 (2012).
Pope, J. E., Deer, T. R., Amirdelfan, W. P., McRoberts, W. P. & Azeem, N. The pharmacology of spinal opioids and ziconotide for the treatment of non-cancer pain. Curr. Neuropharmacol. 15, 206–216 (2017).
Pope, J. E. & Deer, T. R. Ziconotide: A clinical update and pharmacological review. Expert Opin. Pharmacother. 14, 957–966 (2013).
Katz, J., Janik, J. E. & Younes, A. Brentuximab vedotin (SGN-35). Clin. Cancer Res. 17, 6428–6436 (2011).
Ganjoo, K. N. & Patel, S. R. Trabectedin: An anticancer drug from the sea. Expert Opin. Pharmacother. 10, 2735–2743 (2010).
Shetty, N. & Gupta, S. Eribulin drug review. South Asian J. Cancer 3, 57–59 (2014).
Tsien, R. Y. The green fluorescent protein. Annu. Rev. Biochem. 67, 509–544 (1998).
Mehmood, Y. What is limulus amebocyte lysate (LAL) and its applicability in endotoxin quantification of pharma products. In Growing and Handling of Bacterial cultures (ed. Mehmood, Y.) (IntechOpen, 2019).
Harris, J. R. & Markl, J. Keyhole limpet hemocyanin (KLH): A biomedical review. Micron (Oxford, England: 1993) 30, 597–623 (1999).
Cook, P. A. The worldwide abalone industry. J. Mod. Econ. 5, 1181–1186 (2014).
Je, J.-Y., Park, S. Y., Hwang, J.-Y. & Ahn, C.-B. Amino acid composition and in vitro antioxidant and cytoprotective activity of abalone viscera hydrolysate. J. Funct. Foods 16, 94–103 (2015).
CONAPESCA, Anuario Estadístico de Acuacultura y Pesca. Gobierno de México https://www.gob.mx/conapesca/documentos/anuario-estadistico-de-acuacultura-y-pesca 2019.
Zhou, D. Y. et al. Simultaneous recovery of protein and polysaccharide from abalone (Haliotis discus hannai Ino) gonad using enzymatic hydrolysis method. J. Food Process. 40, 119–130 (2016).
Suleria, H. A., Masci, P. P., Gobe, G. C. & Osborne, S. A. Therapeutic potential of abalone and status of bioactive molecules: A comprehensive review. Crit. Rev. Food Sci. Nutr. 57, 1742–1748 (2017).
Yim, S. K. et al. Inhibition of SARS-CoV-2 virus entry by the crude polysaccharides of seaweeds and abalone viscera in vitro. Mar. Drugs 19, 219 (2021).
Kim, T. H. et al. Anti-allergic effect of low molecular weight digest from abalone viscera on atopic dermatitis-induced NC/Nga. Mar. Drugs 19, 634 (2021).
Hao, G. et al. Effect of temperature on chemical properties and antioxidant activities of abalone viscera subcritical water extract. J. Supercrit. Fluids 147, 17–23 (2019).
Ko, S. C. et al. Anti-allergic effects of a nonameric peptide isolated from the intestine gastrointestinal digests of abalone (Haliotis discus hannai) in activated HMC-1 human mast cells. Int. J. Mol. Med. 37, 243–250 (2016).
Nguyen, M. H. T., Qian, Z.-J. & Jung, W.-K. Beneficial effect of abalone intestine gastro-intestinal digests on osteoblastic MG-63 cell differentiation. J. Aquat. Food Prod. Technol. 23, 436–446 (2014).
Li, J., Tong, T., Ko, D.-O. & Kang, S.-G. Antithrombotic potential of extracts from abalone, Haliotis discus Hannai Ino: In vitro and animal studies. Food Sci. Biotechnol. 22, 471–476 (2013).
Li, G. et al. A novel glycosaminoglycan-like polysaccharide from abalone Haliotis discus hannai Ino: Purification, structure identification and anticoagulant activity. Int. J. Biol. Macromol. 49, 1160–1166 (2011).
Lee, C.-G. et al. Abalone visceral extract inhibit tumor growth and metastasis by modulating Cox-2 levels and CD8+ T cell activity. BMC Complemen. Altern. Med. 10, 60 (2010).
Zhu, B.-W. et al. Antioxidant activity of sulphated polysaccharide conjugates from abalone (Haliotis discus hannai Ino). Eur. Food Res. Technol. 227, 1663–1668 (2008).
Li, X.-X., Han, L.-J. & Chen, L.-J. In vitro antioxidant activity of protein hydrolysates prepared from corn gluten meal. J. Sci. Food Agric. 88, 1660–1666 (2008).
Wang, J., Zhang, Q., Zhang, Z. & Li, Z. Antioxidant activity of sulfated polysaccharide fractions extracted from Laminaria japonica. Int. J. Biol. Macromol. 42, 127–132 (2008).
Toyosawa, T., Sasaki, M., Kato, Y., Yamada, H. & Terada, S. Development of A novel serum-free cryopreservative solution. In Cell Technology for Cell Products (ed. Smith, R.) 225–231 (Springer, Netherlands, 2007).
Kiran, N., Siddiqui, G., Khan, A. N., Ibrar, K. & Tushar, P. Extraction and screening of bioactive compounds with antimicrobial properties from selected species of mollusk and crustacean. J. Clin. Cel. Immunol. 5, 1–5 (2014).
Sunil Kumar, D., Janakiram, P., Murali Krishna Kumar, M. & Krishna Geetha, G. Inhibitory activity of bio-active compounds isolated from Anadara granosa in shrimp health management. World J. Microbiol. Biotechnol. 33, 207 (2017).
Khan, S. B., Kong, C. S., Kim, J. A. & Kim, S. K. Protective effect of Amphiroa dilatata on ROS induced oxidative damage and MMP expressions in HT1080 cells. Biotechnol. Bioprocess. Eng. 15, 191–198 (2010).
Nguyen, V. T., Qian, Z. J. & Jung, W. K. Abalone Haliotis discus hannai intestine digests with different molecule weight inhibit MMP-2 and MMP-9 expression in human fibrosarcoma cells. Fish. Aquatic Sci. 15, 137–143 (2012).
Reel, B. et al. The regulation of matrix metalloproteinase expression and the role of discoidin domain receptor 1/2 signalling in coledronate-treated PC3 cells. J. Cancer 6, 1020–1029 (2015).
Fredrich, M. & Illing, R. B. MMP-2 is involved in synaptic remodeling after cochlear lesion. Neuroreport 21, 324–327 (2010).
Carrasco-Jiménez, M. S. & Paz-Cruz, J. A. Tratado de emergencias médicas II, A., Ed. Madrid, 2000; pp 1468–1470.
Bradford, M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing protein-dye binding. Anal. Biochem. 72, 248–254 (1976).
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265 (1951).
Laemmli, U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685 (1970).
Fothergill, A. W. Antifungal susceptibility testing: Clinical laboratory and standards institute (CLSI) methods. In Interactions of Yeasts, Moulds, and Antifungal Agents: How to Detect Resistance Vol. XIV (ed. Hall, G. S.) 170–200 (Springer, 2012).
Valgas, C. S., Machado-Souza, S. & Sania, E. F. A. Screening methods to determine antibacterial activity of natural products. Braz. J. Microbiol. 38, 369–380 (2007).
Van-Ta, Q., Kim, M. M. & Kim, S. K. Inhibitory effect of chitooligasaccharides on matrix metalloproteinase-9 in human fibrosarcoma cells (HT1080). Mar. Biotechnol. 8, 593–599 (2006).
Hou, Y., Wu, Z., Dai, Z., Wang, G. & Wu, G. Protein hydrolysates in animal nutrition: Industrial production, bioactive peptides, and functional significance. J. Anim. Sci. Biotechnol. 8, 24 (2007).
Sukkhown, P., Jangchud, K., Lorjaroenphon, Y. & Pirak, T. Flavored-functional protein hydrolysates from enzymatic hydrolysis of dried squid by-products: Effect of drying method. Food Hydrocoll. 76, 103–112 (2018).
García-Moreno, P. J. et al. Functional, bioactive and antigenicity properties of blue whiting protein hydrolysates: Effect of enzymatic treatment and degree of hydrolysis. J. Sci. Food Agric. 97, 299–308 (2017).
Neves, A. C., Harnedy, P. A., O’Keeffe, M. B. & FitzGerald, R. J. Bioactive peptides from Atlantic salmon (Salmo salar) with angiotensin converting enzyme and dipeptidyl peptidase IV inhibitory, and antioxidant activities. Food Chem. 1, 396–405 (2017).
Tavano, O. L. Protein hydrolysis using proteases: An important tool for food biotechnology. J. Mol. Catal. B Enzym. 90, 1–11 (2013).
Ettelaie, R., Zengin, A. & Lishchuk, S. V. Novel food grade dispersants: review of recent progress. Curr. Opin. Colloid Interface Sci. 28, 46–55 (2017).
Lee, G. & Maruyama, S. Isolation of HIV-1 protease-inhibiting peptides from thermolysin hydrolysate of oyster proteins. Biochem. Biophys. Res. Comm. 253, 604–608 (1998).
López-Abarrategui, C. et al. Functional characterization of a synthetic hydrophilic antifungal peptide derived from the marine snail Cenchritis muricatus. Biochimie 94, 968–974 (2012).
Jeyasanta, I., Sathish, N. & Patterson, J. Identification of bioactive peptides in mussel species of Kanyakumari Coast. Asian J. Biotechnol. 12, 75–86 (2020).
Kanagasabapathy, S., Samuthirapandian, R. & Kumaresan, M. Preliminary studies for a new antibiotic from the marine mollusk Melo melo (Lightfoot, 1786). Asian Pac. J. Trop. Med. 4, 310–314 (2011).
Periyasamy, N., Srinivasan, M. & Balakrishnan, S. Antimicrobial activities of the tissue extracts of Babylonia spirata Linnaeus, 1758 (Mollusca: Gastropoda) from Thazhanguda, southeast coast of India. Asian Pac. J. Trop. Med. 2, 36–40 (2012).
Borquaye, L. S., Darko, G., Ocansey, E. & Ankomah, E. Antimicrobial and antioxidant properties of the crude peptide extracts of Galatea paradoxa and Patella rustica. Springerplus 4, 500 (2015).
Ulagesan, S. & Kim, H. J. Antibacterial and antifungal activities of proteins extracted from seven different snails. App. Sci. 8, 136 (2018).
Montero-Alejo, V. et al. Panusin represents a new family of β-defensin-like peptides in invertebrates. Dev. Comp. Immunol. 6, 310–321 (2017).
Moravej, H. et al. Antimicrobial peptides: Features, action, and their resistance mechanisms in bacteria. Microb. Drug Resist. 24, 747–767 (2018).
Shai, Y. Mechanism of the binding, insertion and destabilization of phospholipid bilayer membranes by α-helical antimicrobial and cell non-selective membrane-lytic peptides. Biochim. Biophys. Acta 1462, 55–70 (1999).
Huang, H. W. Action of antimicrobial peptides: Two-state model. Biochemistry 39, 347–8352 (2000).
Genova-Kalou, P. et al. Cytotoxic and apoptogenic properties of the hemocyanin derived from the marine mollusk Rapana thomasiana in vitro. Planta Med. 74, PA319 (2008).
Umehara, M., Negishi, T., Tashiro, T., Nakao, Y. & Kimura, J. Structure-related cytotoxic activity of derivatives from kulokekahilide-2, a cyclodepsipeptide in Hawaiian marine mollusk. Bioorganic Med. Chem. Lett. 22, 7422–7425 (2012).
Rangasamy, L. et al. Molecular imaging probes on matrix metalloproteinase inhibitors (MMPIs). Molecules (Basel, Switzerland) 24, E2982 (2019).
Huang, H. Matrix metalloproteinases-9 (MMP-9) as a cancer biomarker and MMP-9. Sensors 18, E3249 (2018).
Laronha, H. & Caldeira, J. Structure and function of human matrix metalloproteinases. Cells 9, 1076 (2020).
Sun, L. Cytotoxic and antioxidant activities of abalone (Halitois discus hannai) extracts. J. Life Sci. 24, 737–742 (2014).
Gong, F. et al. A novel peptide from abalone (Haliotis discus hannai) to suppress metastasis and vasculogenic mimicry of tumor cells and enhance anti-tumor effect in vitro. Mar. Drugs 17, 244 (2019).
Qian, Z. J., Ryu, B., Park, W. S., Choi, I. W. & Jung, W. K. Inhibitory effects and molecular mechanism of an anti-inflammatory peptide isolated from intestine of abalone, Haliotis discus hannai on LPS-induced cytokine production via the p-p38/p-JNK pathways in RAW264.7 macrophages. J. Food Nutr. Res. 4, 690–698 (2016).
To CONACyT (Consejo Nacional de Ciencia y Tecnología) for the scholarship awarded to XPPG, to Bertha O. Arredondo Vega for the complementary scholarship awarded to XPPG, to Moisés Martínez Vázquez (CIATEJ, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, México) for the facilities granted to XPPG to carry out the research stay where the bioassays with cell lines were performed; Arturo Pedro Sierra Beltrán for technical support and the facilities provided to carry out the chromatographic analyses at CIBNOR (Centro de Investigaciones Biológicas del Noroeste, S.C.) Proteomics Laboratory; Diana Fischer for English edition. The APC was funded by the Fisheries Ecology Program of the CIBNOR.
The authors declare no competing interests.
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Rivera-Pérez, C., Ponce González, X.P. & Hernández-Savedra, N.Y. Antimicrobial and anticarcinogenic activity of bioactive peptides derived from abalone viscera (Haliotis fulgens and Haliotis corrugata). Sci Rep 13, 15185 (2023). https://doi.org/10.1038/s41598-023-41491-w