Unveiling the influence of the Italian mafia as a Dark Triad threat on individuals’ affective states and the power of defense mechanisms

The present research investigated whether the Italian mafia as a Dark Triad threat increased threat-related affective states and explored how thinking about defense mechanisms may help to reduce these states. For this, we conducted a multi-method experimental study with Italians (N = 253). The quantitative results show that the mafia as a threat manipulation increased threat-related affective states in terms of higher behavioral inhibition (BIS) and lower behavioral activation (BAS). The qualitative results further depict proximal and distal defense mechanisms to reduce this threat, which can be categorized into models of threat and defense. Exploratory analyses indicate that naming distal defenses positively affected the increase of BAS. Additionally, when participants had higher levels of BIS after the threat, naming more defenses and proximal defenses positively affected the decrease of BIS. Further qualitative results provide valuable information on effective personal and societal buffers for the perceived threat of the Italian mafia.

By using violence, attacks, or different techniques of assertion the Italian mafia has become famous for its ruthless and reckless behavior. Quotes attributed to (former) Italian mafia members further illustrate the mafia's willingness to use force, such as "You can get much further with a kind word and a gun than you can with a kind word alone" (Al Capone) and "I can't stand squealers, hit that guy!" (Albert Anastasia).
Mafia members therefore resort to violence, murder, and/or other coercive and unethical means -a behavior that makes them rise in the command hierarchy or maintain their position of power 16 . This kind of behavior, such as relational devaluation (e.g., power games, megalomania, or displacement of guilt), can be threatening to one's basic needs, emotional well-being, and physical health 17,18 . Furthermore, such ruthless and reckless behavior to gain power can also be perceived as a Dark Triad threat 19 .
Due to its destructive and demoralized behavior, the DT can be perceived as a possible threat, leading to their victims' alertness, anxiety, avoidance, and inhibition 19 . This change in affective states can be explained by the Reinforcement Sensitivity Theory: According to the General Process Model of Threat and Defense by Jonas et al. 47 , when people are confronted with an anticipated threat (e.g., something that might be coming and could harm you), they want to avoid it and are conflicted with what to do, leading to high vigilance, avoidance, and/or arousal. Thus, people confronted with an anticipated threat respond with a higher activation of the behavioral inhibition system (BIS) (e.g., higher affective states of inhibition and anxiety) and a lower activation of the behavioral approach system (BAS) (e.g., lower affective states of goal-orientation and relaxation) 47,48 . This process differs from people being confronted with an actual threat (e.g., the lion in front of me): Then the Fight-Flight-Freeze system (FFFS) sets on, leading to either flying out of the threating situation, fighting against the threat, or freezing/frighting/fainting as a kind of staying in an inhibited, hyper-vigilant, and hyper-anxious state 49 . The mafia as an anticipated DT threat (the possibility of having contact with the Italian mafia) could therefore lead to higher BIS (anxiety, inhibition, and avoidance) and lower BAS (goal-orientation, relaxation) and as an actual threat (the mafia boss in front of me) lead to fight/flight/freeze/fright/faint.
If BIS activation can be decreased or BAS activation can be increased, the BAS instead of the BIS sets on, helping with concrete or abstract solutions. This BAS activation leads to different distal defense mechanisms that can either directly deal with the threat (concrete distal defenses), such as by doing something against the mafia, or it can indirectly deal with the threat (abstract distal defenses), such as thinking about better times. Such abstract and concrete distal defenses cannot only be on a personal level as the examples showed but also on a social level: In other words, regarding a concrete defense, other people, such as family members or institutions, can be involved in the defense, or regarding an abstract defense, shared worldviews can be involved 47,48 . Figure 1 summarizes the complete process from threat to defense with its various forms of defense mechanisms.
To summarize, the mafia can be a DT threat, which could lead to higher BIS and lower BAS activation as well as proximal and distal defense mechanisms. To explore this assumption, the present research investigated whether the mafia as a DT threat leads to higher BIS and lower BAS activation, and how people deal with this threat when it comes to defense mechanisms. As the mafia could be considered as DT (e.g. 45 ) we used the Italian mafia as a DT manipulation controlled by a DT manipulation check. Given that the DT can be perceived as a threat, increasing BIS and lowering BAS (e.g. 19 ), we further hypothesized that this manipulation leads to this respective change in affective states. As thinking about defense mechanisms after a threat should again decrease BIS and increase BAS (e.g. 50 ), we further expected a shift back. Thus, our first research question concerns the measured change in BIS and BAS after the mafia DT threat (Research question 1), concluding with the following two hypotheses: H1a BIS increased after the mafia threat and decreased after thinking about the defense mechanisms. www.nature.com/scientificreports/ H1b BAS decreased after the mafia threat and increased after thinking about the defense mechanisms.
In a second research question, we further wanted to explore which defense mechanisms help best when confronted with a mafia DT threat (Research question 2). For this question, we inductively qualitatively analyzed the defense mechanisms people used after being confronted with the mafia DT threat as well as categorized them into the General Process Model of Threat and Defense 47 as a first step. As a second step, we explored their relationship to the BIS and BAS affective states after the threat versus after the defense mechanisms to investigate the change of BIS and BAS dependent on the defense mechanisms named.
Lastly, a third research question qualitatively conducted possible buffers for such a threat situation. In other words, we investigated the societal and personal resources that could help to buffer the DT threat of the Italian mafia (Research question 3), as the process from threat to defense can be buffered by the situational and personal context 47 . This multi-method research approach gives valuable insights into how people react to the mafia perceived as a DT threat and which defense mechanisms can be used to effectively reduce this perceived threat.

Manipulation check.
To check whether the mafia was perceived as a DT threat in our sample, we analyzed both the DT statements and the open answer fields (see "Methods" section). Concerning the DT statements, an average of 5.65 (SD = 2.67) of the 12 statements were clicked on. The inductive qualitative data analysis revealed that the Italian mafia is perceived to be present in Italy (n = 236), although it was described by some participants to be less visible now than in the past (n = 35). Especially the mafia's demand for protection money and threats were often mentioned by the participants (n = 196) but also its infiltration into the social and political system (n = 79), negatively affecting the Italian economy and society (e.g., less economic growth) (n = 82) and leading to a corrupt report system (n = 38). This made participants feel scared (n = 172), angry (n = 86), helpless (n = 82), inhibited (n = 51), alone (n = 19), and frustrated (n = 46). Furthermore, the Italian mafia was described as a criminal and deadly organization (n = 49). Hence, considering both the quantitative and qualitative results the manipulation of this study worked well and the mafia was perceived as DT threat.

Research question 2.
Qualitative inductive analysis. At first, the defense mechanisms were qualitatively analyzed and categorized into the General Process Model of Threat and Defense 47 . As depicted in Table 1, participants named several proximal and distal defense mechanisms with the most named being report/denounce them (n = 105; #23: "I would denounce it whatever it takes"), be afraid (n = 36; #7: "I would be afraid for my safety and the safety of my family members"), and give in/acquiesce everything (n = 36; #132: "I would try to   www.nature.com/scientificreports/ acquiesce to their requests"). Based on the responses, which included a variety of fight, flight, and freeze/fright/ faint strategies, we decided to further categorize them into the FFFS as part of the Reinforcement Sensitivity Theory 49 , although the FFFS should only be triggered in an actual threat situation involving fear 51 . By categorizing the responses into the FFFS, we were able to discover an additional defense mechanism that can neither be described as fight, flight, or freeze/fright/faint. We have described it as 'giving in' as succumbing to the demands of the mafia (n = 31).
Mixed-method-approach. As shown in Table 2, similarly to BIS and BAS (see "Methods" section), the number of qualitative answers for distal defenses and proximal defenses negatively correlated (r = − 0.43, p < 0.001). Furthermore, BIS (quantitative measure) was associated positively with the number of qualitative answers for proximal defenses and negatively with the number of qualitative answers for distal defenses, with a significant association between BIS after Threat and naming proximal defenses. Similarly, BAS was positively correlated with naming distal defenses and negatively with naming proximal defenses, with a significant correlation between naming distal defenses and BAS after Defenses .
To exploratively investigate what kind of defense mechanisms might have influenced the change in BIS and BAS after the threat to after writing defenses, we ran a series of regression analyses using different defense mechanisms as predictors and the z-standardized BIS after threat or BAS after threat as covariates. As presented in Table 3, the results show that naming distal defenses and, in particular, personal distal defenses had a significant positive effect on BAS after threat . In other words, the more distal defenses and, in particular, personal distal defenses were named, the higher the increase in BAS. The other defense mechanisms did not significantly influence the changes in BIS or BAS.
Based on a repeated measures model approach according to Wan 52 , we further examined the moderating role of BIS after threat or BAS after threat in the relationship between the defense mechanisms named and BIS after defense or BAS after defense . As shown in Table 4, BIS after threat moderated the effect of the sum of defenses, proximal defenses, arousal, and freeze/fright/faint on BIS after defense , meaning that participants with higher levels of BIS after threat in comparison to those with average or lower levels experienced a significant decline in BIS from after the threat to after writing defenses when they named more defenses and, in particular, more proximal defenses. Additionally, we found that BAS after threat moderated the relationship between give-in and BAS after defense , meaning that participants Table 1. Proximal and distal defense mechanisms mentioned. Numbers behind the categorization symbolize the number of participants that named this category.

Research question 3. Qualitative inductive analysis.
As the process from threat to defense can be buffered by the situational and personal context 47 , we further qualitatively inductively investigated which societal and personal resources could help to buffer the DT threat of the Italian mafia. About half of the participants expressed trust in the police and suggested reporting the threat (n = 131; #199: "go to the authorities and report the threat"), while others wished for more effective law enforcement and legality in politics (n = 44; #193: "More assistance and real protection by law enforcement"; #10: "Actively monitor these individuals who are dangerous and harmful to society"; #42: "Even in law enforcement there are individuals who cooperate with the Mafia"; #248: "Politics made by righteous people who believe in legality"; #224: "The state doesn't always guarantee your protection"). Participants further mentioned the important role of information (n = 41; #4: "Raise awareness with social media about these issues"; #8: "Talk about what the Mafia is and how to fight it"; #27: "Raise awareness in the community about how shameful, as well as illegal, it is to collaborate with the mafia"; #234: "Educating the younger generation"; #189: "Spread the word among young people, make the younger ones open their eyes with school events and other initiatives"). In addition, participants called people to support social movements against the mafia, such as Addio Pizzo or Libera (n = 56; #125: "Join a movement to fight it together"; #152: "Join the Addio Pizzo movement"; #219: "Support associations like Libera"). Moreover, they encouraged people to refuse to pay (n = 44; #20: "Everyone should resist"; #21: "Refuse to pay lace") and ask for help (n = 5; #9/#114/#131: "Ask for help"). Contrariwise, a few people suggested flight (n = 39), such as "mov[ing] to another state" (#16/#62 /#69/#94/#108/#115/#126/#129/#171/#192/#208/#215/#225). Further mentions included the importance of job creation to reduce poverty and create alternatives for joining the mafia (n = 5; #14: "Giving jobs to young people in exploited areas"), the importance of psychological support (n = 3; #209: "Social and psychological assistance"), and the importance to "stop idolizing the mafia" (#51).

Discussion
The Italian mafia is known for its ruthless and reckless behavior, for which they are frightened by the Italian population 8,9 . However, research on how the population deals with the threat of the mafia is rare. Building on previous studies that showed a link between the mafia and DT tendencies (e.g. 42,45 ), the present multi-method research explored (1) whether the mafia perceived as a DT threat is leading to higher BIS and lower BAS activation (quantitative approach), (2) how people deal with this threat when it comes to defense mechanisms as well as how this relates to BIS and BAS activation (mixed-method approach), and (3) what could be done on an individual and societal level to buffer the perceived threat (qualitative approach). Concerning Research question 1, the results of this study showed that BIS increased and BAS decreased after the threat of the mafia as well as BIS decreased and BAS increased after thinking about the defense mechanisms. This result is in support of our hypotheses H1a and H1b as well as in line with previous threat research (e.g. 50 ).
Concerning the qualitative part of Research question 2, the results of our investigations of the defense mechanisms were in line with the General Process Model of Threat and Defense 47 . Additionally, participants reported fight, flight, and freeze/fright/faint strategies that can be categorized into the FFFS 49 . While the FFFS should be only activated in actual but not anticipated threat situations 51,53 , there are two assumptions that can be discussed: Firstly, the mafia threat might have fueled both processes of anxiety and fear, as Italians might have perceived this threat so real and physically close-an assumption that can be supported by previous research on how threats can be perceived closer as they are 54 . Secondly, this finding fuels the ambivalent debate about how different these two processes really are 51,55 . Table 1 provides a cross-over design that depicts how both processes can be connectively qualitatively researched. Furthermore, the Reinforcement Sensitivity Theory Affects Questionnaire (RST-AQ) provides a quantitative option to combine both processes 51 .
Furthermore, one personal distal defense mechanism, i.e. "giving in", was named that cannot be categorized into the FFFS 49 . One explanation of why the strategy of giving in was shown is that DT threats might lead to feelings of helplessness 19 , which is why this could be a sign for learned helplessness (e.g. 56 ). Another explanation of this finding is the dual concern model that suggests five ways of how people deal with a conflict: (a) to compete, force and fight, (b) to compromise in terms of finding a deal or collaborate in terms of finding the best solution for both parties, (c) to avoid the conflict, and (d) to accommodate / give in. This last strategy of giving in is mainly used when there is a higher concern towards the other party than towards one's own goals 57 -particularly when this party is powerful: "Accommodation often occurs when there is a power differential between the parties and the high power party is willing to use a forcing approach to obtain what he or she wants. Realizing that the situation is futile, the low power party accommodates to the high power party, limiting any damage to the relationship or the organization" 58 (p. 72).
Regarding the mixed-method part of Research question 2, we firstly discovered a positive relationship between BIS and proximal defenses, as well as a negative relationship between BIS and distal defenses. Similarly, we observed a positive relationship between BAS and distal defenses, as well as a negative relationship between BAS and proximal defenses. This finding is in line with the General Process Model of Threat and Defense (see Fig. 1 47 ). By taking a closer look, we further found that naming more distal defenses and, in particular, more personal distal defenses seemed to help increase BAS. This finding is in line with the model, as personal concrete distal defense strategies help regain "personal control or self-efficacy [as well as can] provide a vision of clear and decisive goal pursuit" 47 (p. 247). In addition, we found that people with higher levels of BIS had success in decreasing their BIS when writing about more defenses in general, independently of what they wrote about. This underlines the importance to exhibit a variety of defense mechanisms when dealing with negative affective states (e.g. 59,60  www.nature.com/scientificreports/ BIS affective states seemed to have helped them feel less anxiously inhibited. Following previous clinical and nonclinical research on the effects of the expression of negative affective states, it can be supposed that the writing has led to enhanced self-acceptance, fostered self-understanding, and reduced inhibition of negative feelings, which, in turn, goes along with reduced feelings of stress (e.g. 61,62 ). Additionally, we found that writing on giving in has helped people who felt lower levels of BAS after threat to increase their approach-oriented affective state. Although affective states can influence motivations and conflict strategies 63 , it is unclear why such a strategy where one gives up their control in some sense (e.g., control theory) can help BAS to regenerate. However, when looking at concrete personal defenses, such defenses do not need to be effective to work and as "compulsive reactions may also conflict with own goals and values" (p. 247) as can be seen by drug use or greedy consumption after threat 47 . In other words, giving in might just be an easy strategy people in their first impulse strive towards.
In terms of possible societal and personal buffers (Research question 3), most people suggested going to authorities, while others criticized that more effective law enforcement and legality in politics are needed. This finding highlights the importance of trust in political actors to keep a political system stable, especially when citizens feel threatened (e.g. [64][65][66] ). Further suggestions for buffers include sharing information about the Italian mafia with the public and supporting movements against the mafia. Previous and recent social movements, such as the Fridays For Future climate protest, have emphasized the valuable opportunities social movements offer by building powerful collectives, raising awareness for the threats, and motivating policy changes before the threatening circumstances even increase (e.g. [67][68][69] ).
Limitations. Although the present research provides valuable findings, there are some limitations to consider. One first limitation of this study is its use of an Italian study sample and its restricted focus on the Italian mafia. However, the Italian mafia's activities are not limited to Italy [70][71][72] and other mafias besides the Italian mafia exist (e.g., Russian mafia 73 ). Thus, future studies should investigate other national or international contexts. A second limitation addresses the use of a scenario as manipulation, as affective states elicited by scenarios can differ from the affective states experienced in a field study design (e.g. 74 ). For instance, a recent study by Baraldi et al. 8 showed the associations between the mafia's organized crime infiltration and women's reduced participation in politics due to feelings of fear. A third limitation concerns our mixed-method analyses with our defense strategies; future research could focus on replicating our findings by measuring possible defense mechanisms quantitatively to better conclude the relationship of these defense mechanisms to the affective states. A fourth limitation addresses the use of a dichotomous scale for the DT manipulation check, as a Likert scale could better quantify the perception of the mafia as a DT threat.

Conclusion.
In conclusion, the Italian mafia as a DT threat and their effect on the Italian population cannot be denied: Not only our results but also the first field studies on the Italian mafia (e.g. 8,9 ) highlight the feelings of anxious inhibition and fear triggered by the Italian mafia. However, our results depict the importance of writing about possible defense mechanisms to reduce these threat-related affective states. One practical implication could therefore be to not only inform about the mafia but also inform about how important it is to think about various defense mechanisms. Teaching citizens to use concrete personal defenses might be especially important to increase their feelings of BAS. Furthermore, our results provided valuable starting points for societal interventions to buffer the process from threat to defense.

Methods
Sample. To calculate the sample size required to explore changes in BIS and BAS over our study, we conducted an a priori power analyses using G*Power 75  Of this sample, 10% reported having friends or family members involved with the mafia, as well as 2% reporting to have themselves been hurt by the mafia, 2% having friends or family hurt by the mafia, and 1% having friends or family killed by the mafia. Other 8% reported not being hurt but a) being otherwise threatened, such as having been locked up by the mafia (#26) , or b) hearing about rumors of people in the neighborhood being threatened, such as hearing about people in the neighborhood being injured (#211). Four participants were excluded from the originally 256 participants because of not speaking Italian (n = 1), not answering the open fields seriously (n = 2), and failing the reading check (n = 1) (a reading check was provided where participants had to write "party [festa]" in order to pass the reading check).

Study design.
Ethical approval for the research was granted by the University of Salzburg as part of the FWF P 27457 project. Additionally, participants actively gave their informed consent to participate in the study at the beginning of the survey. All methods were performed in accordance with the relevant guidelines and regulations. An online survey (Limesurvey) in the Italian language was sent out via social networks and via MTurk [There was no difference in the participants 'answers when controlling for this variable as well as no time differences with regard to completing the survey. Participants recruited via Mturk received 1 Euro for their participation]. The survey can be found under the OSF link provided (see Data Availability Statement). The research was promoted as an online study investigating feelings and perceptions with regard to institutions. The survey started with agreeing to an informed consent, followed by BIS and BAS affective state measures. Then, the mafia threat was introduced and participants were asked about their perception of the mafia. Next, BIS and BAS affective www.nature.com/scientificreports/ states were measured again. After this, questions on defense mechanisms were asked-again followed by BIS and BAS affective states measures. In the end, demographic data, questions about participants' Covid-19-related fears (as data was collected in 2021), and the possibility to leave a comment were collected. On the thank-you page, participants were debriefed.

Measures. Mafia manipulation.
To make the DT threat salient, participants reflected on the mafia in Italy with regard to four open fields in a way of how a threat has been manipulated as a common practice in threat research (e.g. 50 ): (1) How prevalent is the Mafia in Italy? (2) What do you think about the Mafia in Italy? (3) Take two minutes to imagine a situation in which you receive a threat from the mob. Then, take the time to write down what you imagined in this situation. Explain the situation in as much detail as possible. (4) Take time to imagine how that situation would make you feel. Then, take time to describe your feelings and emotions related to that event. (translated version).
Manipulation check: DT threat. A first manipulation check was the qualitative analysis of the open questions on the mafia to see whether it was perceived as a threat. To specifically address the DT aspect of the threat, we adapted the twelve items of the Dirty Dozen Scale by Jonason and Webster 76 to the mafia context (e.g., our item: "In my opinion, the Mafiosi are used to expect special favors from others"; original item: "I tend to expect special favors from others "; see Supplementary Appendix 3), as this measure is invariant when assessing DT personality traits in diverse clinical as well as non-clinical groups 77 . As we needed a selective answer (would they categorize mafia as DT yes or no), we used yes or no as answer options instead of a Likert scale. This way, we can better categorize the mafia as either a DT threat or not (see similar procedures, e.g. 78 ). The scale had an acceptable internal consistency of α = 0.69.

BIS and BAS.
The BIS scale consisted of four items, namely inhibited, worried, restless, and insecure. The first three items have been already used by other scholars (e.g. 79,80 ; see Supplementary Appendix 4) to measure BIS. As a perceived threat can go along with feelings of insecurity which can also be understood as a higher activation of the behavioral inhibition system (e.g. 81 ), we further decided to add the item "insecure" to our scale (α = 0.91-0.93). To assess BAS, we used the items relaxed and cooperative by Greenaway et al. 82 for measuring low approach orientation as well as an adapted version for measuring high approach orientation based on Greenaway et al. 82 and Reiss et al. 80 82 participants were asked to indicate the extent to which this series of adjectives describe their current feelings from 1 (fully disagree) to 10 (fully agree). An overall score was computed for BIS and BAS as an average of all four or six items (see correlations in Table 5).

Defense mechanism.
An open question assessed how participants would act as a result of the mafia threat to activate defense mechanisms: "How do you think you would act as a result of the imagined situation? Take the time to imagine how you would behave as a result of the imagined situation". The answers were qualitatively inductively analyzed and then categorized into the General Model of Threat and Defense 47 .
Possible buffers. In addition, we asked participants about possible buffers for the mafia threat: "Now we would like you to think of some ways to deal with the Mafia in Italy. Describe below all the ways you think the Mafia can be confronted". The answers were qualitatively inductively analyzed.
Additional measure: Covid-19-related fears. For research outside the present study, we further collected data on participants' Covid-19-related fears using the MAC-FR scale by Schimmenti et al. 83 (α = 0.77). Participants were asked to share their sense of agreement from 1 (fully disagree) to 5 (fully agree) to seven statements, such as "I am frightened about my body being in contact with objects contaminated by the coronavirus". As this measure is out of the scope of the present research questions, it was not considered in the following analyses.
Data analysis. All quantitative data analyses were performed with R 84 using the packages afex 85   www.nature.com/scientificreports/ according to Mayring 89 were conducted: (1) Two inter-coders were introduced to our research questions and information on the theoretical background of the research topic was given. (2) The selection criteria, the category definitions, and the level of abstraction (low) were explained to them. (3) The coders worked through the open answers given by the participants and extracted categories from them. (4) After working through 10% of the text materials a revision was made. (5) Afterwards, the text materials were completely worked through. (6) Main categories were created with regard to the Anxiety-to-Approach model; (7) then, an inter-coder agreement check followed. The coders' results were compared and adjusted. To ensure high reliability, a subcategory was coded only if both coders agreed. (8) In the last step, a review of the final results, the calculation of the frequencies of the extracted categories, and the interpretation of the data followed.
Ethical approval. The study was included in the FWF project FWF P 27457 and all studies in this project were approved by the Ethics Committee of the University of Salzburg.

Data availability
The data that supports the findings of this study are openly available to the reviewers in Open Science Framework under the following link: https:// osf. io/ rpqyc/? view_ only= 179ac 1ba65 f34d9 e8ba3 0a40c 117d6 77. This data has not been published elsewhere but can be made openly available after publishing this manuscript to allow further research at https:// doi. org/ 10. 17605/ OSF. IO/ RPQYC. www.nature.com/scientificreports/