Hippocampal inactivation during rearing on hind legs impairs spatial memory

Spatial memory requires an intact hippocampus. Hippocampal function during epochs of locomotion and quiet rest (e.g., grooming and reward consumption) has been the target of extensive study. However, during navigation rats frequently rear up onto their hind legs, and the importance of hippocampal activity during these periods of attentive sampling for spatial memory is unknown. To address this, we tested the necessity of dorsal hippocampal activity during rearing epochs in the study phase of a delayed win-shift task for memory performance in the subsequent test phase. Hippocampal activity was manipulated with closed-loop, bilateral, optogenetic inactivation. Spatial memory accuracy was significantly and selectively reduced when the dorsal hippocampus was inactivated during rearing epochs at encoding. These data show that hippocampal activity during periods of rearing can be important for spatial memory, revealing a novel link between hippocampal function during epochs of rearing and spatial memory.

Apparatus. Training took place on a custom built automated 8-arm radial maze. The maze had a 33.2 cm wide hub and white opaque pneumatic drop doors at the entrances to the 8 arms. The arms measured 48.26 cm long, 10.79 cm wide, and had opaque white acrylic floors. The arms had 20.95 cm tall clear acrylic walls allowing for viewing of distal cues surrounding the maze at a range of distances from one to five feet from the maze (Fig. 1b). Cues were constructed from brightly colored tape or construction paper with a row of 6 cues evenly spaced level with walls of the maze, and a second set row of cues approximately 2 feet above the first row of cues. The room also contains a framed image, a desk, and a bookshelf. At the end of each arm were food wells in which 45 mg sucrose pellets (Bio-Serv, Flemington, NJ) were delivered. The maze was open on top to permit testing of tethered animals. The maze was cleaned with chlorhexidine immediately after each trial.  www.nature.com/scientificreports/ Experimental design. The experimental design was a 2 × 3 design with two levels for opsin expression {opsin + reporter (Experimental Group), reporter only (Control group)} manipulated between animals and three levels for light delivery timing {'' Off ' , 'Rear' , 'Delay'} manipulated within animal. Manipulations of light delivery timing served to test the relevance of hippocampal activity during rearing behavior. 'Off ' established the baseline ability of each rat to complete the task. 'Rear' measured the impact of timing light delivery to be synchronous with rearing and represented the key experimental condition. 'Delay' controlled for the total amount of light delivered within a trial but desynchronized the light delivery from the rearing behavior. Each rat completed 6-14 trials of each optogenetic condition in pseudo random order (Supplementary Tables S1 and S2 indicate the number of trials per rat per condition). Each rat completed only one trial per day. The only constraint on the order was that all three conditions be tested before any was repeated another time. Manipulations of opsin expression served to isolate the effect of the opsin on behavior by controlling for any incidental effects of light delivery or viral transfection. The opsin expression conditions formed two cohorts of rats, one of which was transfected with the opsin and fluorescent reporter referred to as the Experimental Group, while the other was transfected with reporter only and is referred to as the Control group.
Behavioral training. Habituation and preliminary training. For 3 days prior to training, rats were handled for 10 min and given 20-30 sucrose pellets to habituate them to experimenter handling and rewards. Preliminary training consisted of one 10 min. trial daily for 4 days. During each, 4-6 pellets were placed along each of the 8 arms, and 2 pellets in the food wells of each arm. The rat was allowed to freely explore the full maze. The trial ended after the rat consumed all pellets or 10 min had elapsed.
Initial training. Initial training consisted of 10 sessions, one trial/session. During each, rats were trained that arms would be baited once per day. Prior to each trial, the food wells of all 8 arms were baited with 2 pellets each. Training trials began by placing the animal in the central hub with hub doors closed. After one minute, the doors opened, allowing the rat to freely forage. The rats could explore until all pellets were collected or 15 min had elapsed.
Task. The spatial memory task used here was the delayed-win-shift task on an 8 arm radial arm maze 41,42 . The task consisted of three phases, a study phase, a delay phase, and a test phase, as shown in Fig. 1. In the study phase, the rat was placed in the central hub. After 30 s, a random set of four doors opened, and the rat was allowed to collect pellets from each. The rat was then removed from the maze and placed on a pedestal next to the maze for a 4 min delay phase. During which, the maze was cleaned with chlorhexidine to remove odor cues. The test phase began by placing the rat in the hub and after 30 s, all eight doors opened. The four arms that had not opened during the study phase baited food wells. The test phase ended after all the pellets had been consumed or when 15 min elapsed. Each rat ran one trial of the task each day of training. Regular training (~ 5 days/ week) continued until rats achieved behavioral criterion: no more than three errors over four days.

Behavioral scoring.
The key planned dependent measures of spatial memory performance were percent correct, and total number of arm entries. Percent correct was measured as the number of the first four choices of the test phase that contained rewards divided by four. Number of arm entries was measured as the total number of arms entered to find all four baited reward sites. A visit to an arm was defined to occur when the rat's hind feet entered the arm. An arm visit counted as an error if the arm had been visited earlier in the trial. Percent correct acts as a measure of spatial reference memory errors (reentry into arm from study) to test memory for arms entered during the study phase. Total number of arm entries reflects spatial working memory errors (reentry into a baited arm) and the degree to which the rat can error correct if they fail to enter the baited arms in the first four choices at test [41][42][43] . Supplemental posthoc analyses of behavioral strategy examined further measures, including (1) Number of reentries = count of the number of times a rat leaves an arm and then reenters the same arm prior to entering other arms within a test phase. (2) Rotational Bias = an examination of entries into arms immediately adjacent to the arm the rat leaves that quantifies how often they turn one direction versus the other. It is calculated as the absolute value of the difference in the number of clockwise and counterclockwise transitions. It ranges from zero to the number of total arm entries. A value of zero indicates a balanced likelihood of transitioning one way versus the other. Values above zero indicate how many more times the rat transitioned in one direction relative to the other. 3) Max sequence length = a quantification of the maximum number of consecutive transitions to adjacent arms in one direction or the other. It can range in value from zero, for never transitioning to adjacent arms, to greater values up to the total arm entries if the rat progressed from one arm to the next around the maze. 4) Number of opposite entries = count of the number of times a rat leaves an arm and then enters the arm immediately across from the exited arm in a test phase (Supplementary Tables S3 and S4).

Rear detection.
Rearing behavior was tracked using a 3D camera (RealSense Depth Camera D435; Intel).
The camera was positioned on the ceiling above the center of the maze facing down such that it was able to capture the whole of the maze at 30 Hz and 640 × 480 resolution (Supplementary Videos S1 and S2). A custom real-time analysis package was written to detect rearing events. Rearing events were defined as moments when a 'blob' of appropriate size entered a region of interest. The region of interest was a 3D zone filling a short and broad cylinder of space above the maze. The cylinder perimeter matched the perimeter of the maze enclosure. The bottom of the cylinder was aligned with the top of the enclosure walls. The top of the cylinder extended 20 cm above this. The top limit was defined to prevent erroneous triggering events from the tether as it moved between the camera and the enclosure. To further prevent erroneous triggering, blobs were required to occupy more than 0.13 cm 2 and less than 26.46 cm 2 . This served to prevent the experimenter from incidentally trigger- www.nature.com/scientificreports/ ing the laser by coming into view of the camera. The rear detection routine was implemented with the Opensource Computer vision's (OpenCV) blob detector. When a blob of appropriate size was detected in the region of interest, a command is sent to an Arduino unit which then activated the laser system. For the control group rats, the 3D camera laser system was operated manually.
Optogenetic light delivery. Optogenetic control was implemented with the eNpHR3.0 halorhodopsin, a light gated ion pump which inhibits neural activity with photostimulation 44 . Activation of halorhodopsin was achieved using light from a CE:YAG laser diode optical head laser system (Doric) filtered to 570 nm Laser output was delivered to the hippocampus by way of a patch fiber connected to a rotary joint (Doric) and, from the rotary joint, a dual fiber optic patch cord (Doric) that was coupled to the implanted optical fibers prior to each testing session. Light intensity was controlled by the Doric Neuroscience studio software to obtain 5-10 mW at the tip of the fiber in the brain using a photodiode power sensor coupled to a power meter (Thorlabs). Laser activation was triggered by an external signal generated by an Arduino unit (Arduino due) that was running custom rearing detection software.
The optogenetic experimental conditions were as follows: in the main experimental condition wherein light was delivered coincidentally with rearing behavior, referred to as 'Rear' in the results, the laser was activated as the 3D camera system detected rearing and remained on for the full duration of the rear. In the baseline 'Off ' condition, the main power switch for the laser remained off so that no light was delivered at any point. Nonetheless, the optical fiber was attached as in the other conditions. In the control 'Delay' condition, light was delivered in response to rearing, but both the laser activation and deactivation were triggered at a fixed 6 s delay relative to the rearing. The 6 s delay was inserted by the control software between the time when the 3D camera system detected rearing and the time when the state of the laser system was switched. Thus, importantly, the duration of light delivery in the 'Delay' condition matched the duration of the detected rear. All rats were coupled to the fiber patch cords during all testing, irrespective of condition or cohort. The three conditions were randomized such that all conditions were run in a random order every 3 trials. Epochs of optogenetic manipulation were restricted to occur in the study phase only. Hippocampal activity was unmanipulated during the test phase when spatial memory was assessed.

Stereotaxic surgery and viral vectors.
Rats in the experimental group underwent two surgeries. In the first, a virus was injected into the dorsal hippocampus. In the second, an optical fiber cannula for light delivery and opsin activation was implanted. During the first surgery, rats were anesthetized with 1.5-4% isoflurane, and their head was positioned in a stereotaxic frame. The scalp was cut and retracted. Three sites were drilled above the hippocampus bilaterally. In each, viral infusions were performed at 3 different depths. Thus, a total of 18 separate 45 nl injections were done at the following coordinates: . The same AAV serotype and promoter have been used to induce opsin expression in the rat hippocampus previously 45 . At the end of surgery, the scalp was sutured shut, and the rat was allowed to recover for one week post surgery before continuing regular behavioral training. Rats underwent the second surgery 2-5 weeks after the first surgery. Again, rats were anesthetized with 1.5-4% isoflurane, their head was mounted in a stereotaxic frame, and the scalp was cut and retracted. Two optical fibers (MFC_200/245-0.53_5mm_MF2.5-FLT; Doric Inc) were positioned above the center injection sites from the previous surgery, at -3.7 AP, 2.9 ML, 1.8 DV, and fixed to two jewelers screws inserted into the skull with dental acrylic. The scalp was sutured closed around the implant. The control group rats underwent one combined viral injection and optical fiber implantation surgery. Only a fluorescent reporter was expressed by infusing the virus AAV(5)-CAMKIIa-EYFP (UNC vector core). One 2 μl injection into each side of the hippocampus [− 3.6 AP, ± 2.8 ML, 2.4 DV] with two optical fibers (MFC_200/245-0.53_5mm_ MF2.5-FLT; Doric Inc) placed bilaterally above the injection site. Again, rats were allowed to recover for one week following surgery before restarting behavioral training. Data collection began after rats reached behavioral criterion. While previous research demonstrates that isoflurane exposure can impair spatial memory [46][47][48] because our results are based on within animal comparisons, this cannot account for the effects observed.
Histology and immunohistochemistry. Upon completion of testing, animals were euthanized via isoflurane overdose and perfused intracardially with phosphate buffered saline (PBS) followed by a 4% paraformaldehyde saline solution. Brains were saturated with a 30% sucrose solution prior to sectioning. Coronal sections (50 μm thick) were cut with a crysostat (Leica Biosystems) or microtome (American Optic company). Immunohistochemistry was performed on free-floating sections to amplify induced EYFP reporter signaling. Sections were first rinsed with PBS then blocked with buffer (PBS, 5% normal goat serum, and 0.4% Trition X-100). This was followed by overnight incubation with conjugated anti-GFP rabbit antibody (1:1000; catalog no. A21311; Invitrogen). Finally, the sections were rinsed with PBS and then mounted on slides and cover slipped with DAPI and Fluoroshield.
Statistical analysis. Because individual animals completed different numbers of testing sessions, statistical analyses were performed with a multilevel hierarchical model to test the relationship between the light off condition and optogenetic stimulation change in measures of interest. The model treated light delivery timing as a fixed effect, and rat identity was set as a random effect. Concretely, the model was implemented with the equation "Perf ~ Opto + (Opto|Rat)" where Perf is the behavioral score of interest (percent correct or number of arms entered), Opto was a categorical variable indicating the light delivery timing condition and Rat was an index indicating the rat identity from which each data point came. Models were analyzed in MATLAB using the www.nature.com/scientificreports/ glme.m function. We sought to determine whether the slope relating to Opto to Perf was significant, that would indicate that the timing of light delivery changed the respective behavioral score. An additional statistical test was run to compare the number of rears of females vs. males collapsed across the 'rear' and 'delay' condition for the control group to test for sex differences, following the same model as above but switching the number of rears for Perf. Results are reported as the expected effects and 95% confidence intervals (e.g., Effect [lower bound, upper bound]) as determined by the hierarchical modeling. Significance was determined based on an alpha level of 0.05.

Results
To determine if hippocampal activity during rearing is important for spatial memory, we performed a closed-loop experiment wherein we tested the effect of optogenetically inhibiting the dorsal hippocampus during epochs of rearing on spatial memory. Spatial memory was assessed with the eight-arm delayed-win-shift task, consisting of study, delay, and test phases (Fig. 1a,b). During the study phase, four of eight arms were opened to the rat, and, in each, the rat found food rewards. In the delay phase, rats were removed from the maze for four minutes. Finally, in the test phase, rats were granted access to all eight arms but could only find rewards in the four previously unopened arms. Rats were trained on this task with one trial per day to criterion level accuracy (< 3 errors in four consecutive days of testing, > 80% accuracy) before and after the viral transfection and fiber cannula implantation surgeries. Viral transfection targeted the bilateral dorsal hippocampus proper and either transduced expression of halorhodopsin and fluorescent reporter (experimental group) or fluorescent reporter only (control group). Fiber optic cannula were implanted bilaterally dorsal to the viral injection (Fig. 1c,d). Three different conditions of optogenetic manipulation were examined (Fig. 1e): Off) wherein no light was delivered, used to assess baseline performance; Rear) light delivery was synchronized to rearing events, used to assess memory performance when hippocampal processing was disrupted selectively during rearing; and Delay) light delivery is delayed by six seconds relative to the onset of a rearing event, used to control for intermittent disruption of hippocampal processing during study but without synchronizing the disruptions to rearing events. Starting with the experimental group, comparing percent correct (Fig. 2a. left bars) at test between the 'Rear' condition (blue bar), wherein light delivery was synchronized to rearing, and the 'Off ' condition (grey bar), wherein no light was delivered, revealed a significant reduction to spatial memory (77.7% vs. 65.7%; GLME est. = − 11.9% [− 21.6%, − 2.3%], t(178) = − 2.44, p = 0.02). The same comparison between the 'Rear' condition and 'Off ' condition, performed on data collected from the control group (Fig. 2a. right bars), wherein halorhodopsin was not expressed, revealed that light delivery had no significant effect on percent correct (81.4% vs. 83%; GLME est. = 2.7 [− 4.1, 6.5], t(217) = 0.4, p = 0.66).
Examining the number of arms entered to find all rewards instead of percent correct revealed the same pattern of results. For the experimental group (Fig. 2b left three bars), rats entered significantly more arms while finding all the rewards on 'Rear' trials than 'Off ' trials (5.1 vs. 6.5; GLME est. Differences between the 'Rear' and 'Off ' conditions could have been simply due to having intermittently disrupted hippocampal activity during the study phase of 'Rear' trials. That is, the 'Rear' versus 'Off ' comparison does address whether it mattered that inactivation was synchronized to rearing. To test if synchronization of  www.nature.com/scientificreports/ the light delivery to rearing was a determining factor in the effects listed above, we also tested whether inserting a six second delay between detected rearing events and the laser activation would also disrupt spatial memory performance. This is the 'Delay' condition. If the memory impairments observed above were simply the result of inactivating the hippocampus at all, irrespective of whether the rat was rearing, then we should expect a significant impairment in the 'Delay' condition as well. However, if the memory impairments resulted because we synchronized the inactivation with rearing behavior, then we would not expect a significant impairment when the laser activation is delayed relative to rearing. The analyses showed that performance in the 'Delay' (green bar) was not significantly different from the 'Off ' condition with regard to percent correct (77.7% vs. 72.0%; GLME est. = − 5.7 [− 14.2, 2.8], t(178) = − 1.3, p = 0.19) (Fig. 2a left bars) and only a trend toward an increase in total arm entries (5.1 vs 5.9; GLME est. = 0.8 [0.0, 1.7], t(178) = 2.0, p = 0.05) (Fig. 2b left bars). Note, this trend is in contrast to the strong effect observed when comparing the 'Rear' condition to the 'Off ' condition. That the inactivation should show any trend is not surprising, but it is also likely that the six second delay did not fully desynchronize light delivery from rearing behavior. A post hoc analysis comparing epochs of rearing and epochs of laser activation revealed that the laser overlapped a mean ± std of 35.5% ± 16.3% of the total rearing in the Delay condition. As expected, but included here for full transparency, the delay condition similarly had no significant effect in the control group for percent correct (81.4 vs 82.4%; GLME est. = 2.6 [− 4.1, 6.3], t(217) = 0.41, p = 0.68) (Fig. 2a right bars) or total arms entered (5.2 vs. 5.5; GLME est. = 0.3 [− 0.2, 0.92], t(217) = 1.17, p = 0.24) (Fig. 2b right bars). 'Delay' condition data are shown in Fig. 2.

Discussion
Here, we sought to test the relevance of rearing as an epoch of hippocampal dependent encoding of spatial memories. To address this, we examined how spatial memory was affected by closed loop optogenetic inhibition of the dorsal hippocampus activity during rearing. The results show that spatial memory performance in the test phase of a delayed radial maze win-shift task decreased significantly when dorsal hippocampus activity was inhibited selectively during rearing in the study phase. Spatial memory impairments were not observed when a control virus that lacked the halorhodopsin gene was used ruling out the possibility that light delivery effects (e.g., tissue heating, visual distraction, etc.) caused the reduced performance in the experimental group. The fact that the 'Delay' condition also didn't generate significant performance impairments indicates that it is not simply inactivating the hippocampus at odd intervals during the study phase that impaired memory. Together, these results demonstrate that dorsal hippocampal activity during rearing is important for spatial memory.
Our results are consistent with the hypotheses of Lever and colleagues regarding the functions of rearing. Having reviewed the limited body of work on rearing, Lever and colleagues 15 synthesized the available data with the hypothesis that 'rearing is a useful marker of environmental novelty, that the hippocampal formation is a crucial component of the system controlling rearing in novel environments, and that rearing is one of several ethological measures that can profitably be used to assess hippocampal learning and memory. ' Strong evidence was available at the time to support their hypothesis that rearing marks environmental novelty: A wide variety of mammals rear in response to environmental novelty. The hypothesized links to hippocampal function were more speculative. The data indicated that lesions of the hippocampus have inconsistent effects on rearing frequency 49,50 . Only indirect evidence existed to link rearing and hippocampal learning. For example, rearing covaried with performance in the Morris water maze-declining during learning and reinstating when the platform is moved, and that hippocampal lesions disrupt this pattern 5,51 . In contrast, the present findings strongly and directly support the hypothesis that rearing is an ethological measure of hippocampal learning.
The current results also advance our understanding of the connections between rearing and hippocampal learning beyond work done on the topic by Wells et al. 18 and Mun et al. 19 since Lever and colleagues published their review. Wells et al. showed that increasing environmental novelty both decreases the speed modulation of hippocampal theta and increases the number of times rats reared, a result that indicated a coupling between hippocampal function and rearing behavior 18 . Mun et al. 19 reported that the discrimination index in a novel place task (a measure of spatial memory) was positively related to rearing frequency during the initial exploration phase. Notably, this effect was specific to the novel place task, a task known to be sensitive to hippocampal integrity, and was not observed in the novel object task, a task that is generally insensitive to hippocampal integrity except at long delays 52,53 . Thus, rearing specifically promoted memory in a hippocampal dependent task. Neither Wells et al. 18 nor Mun et al. 19 tested the necessity of hippocampal activity during rearing for spatial memory. The results of our experiment are an advance over these works by showing explicitly that hippocampal activity during rearing is important for spatial memory.
While the function of rearing for spatial memory is not known, prior work suggests that the inactivation we performed in the current experiment may have disrupted spatial memory by interfering with updating of an internal model of the environment. Rearing is likely a form of active environmental sampling. As it relates to spatial memory, rearing has been suggested to aid in building and updating a model of the environment 15,24 . Rearing frequency is increased by cue changes that elicit hippocampal remapping 54 . Barth et al. 24 suggested, based on analyses of hippocampal field potentials and unit activity, that the hippocampus switches to a distinct functional mode during rearing. This mode, they suggest, draws on sensory information gathered during the rearing to reduce uncertainty regarding the allocentric position and to perform sensory realignment of the cognitive map 24 . By inactivating the hippocampus during rearing, our manipulation may have prevented this updating with downstream consequences for spatial memory.
We note, however, that any updating that is happening in our experiment is unlikely to be de novo environmental modeling. Prior to testing, while reaching performance criterion, our rats completed dozens of trials under identical circumstances providing ample time to model the environment proper. Importantly, however, www.nature.com/scientificreports/ the set of arms that opened during study (and which were still baited at test) varied randomly each day. Thus, the challenge on any given day was to disambiguate current trial information from the proactive interference of prior training. For this reason, we expect that any updating being performed would be to support trial specific event memory. This resembles the 'Where did I park my car today?' type memory, requiring disambiguation of recent events from similar prior events, that characterizes hippocampal function 55 . Alternatively, as noted by Lever et al., rearing may reflect updating that is driven by the change of rewards in an established, stable spatial structure a possible and likely context in which foraging behavior commonly occurs. The radial arm maze is in fact designed to model foraging behavior, where new rewards and their relation to the established external distal cues are efficiently understood not by engaging in normal horizontal ambulation but in this context by rearing. The distal cues can help disambiguate arms for which rewards have or have not yet been consumed. Indeed these results provide direct evidence for the proposal by Lever et al. 2006 that rearing may be in some contexts, more efficient than horizontal locomotion in the determination and updating of spatial information 15 . The current work does not address if a specific portion of the hippocampus proper is necessary for spatial memory encoding during rearing. However, prior work suggests the dentate gyrus may be important 24,56,57 . Spatial memory ability in radial maze tasks is correlated to the size of mossy fiber terminal fields 56 . Separately, it was found that selectively breeding mice for frequent rearing behavior resulted in progeny with increased mossy fiber terminal fields 57 . Lesions to the dorsal dentate gyrus block re-exploration of objects or the environment with rearing after stimuli or environmental change 58 . The relevance of the dentate gyrus for driving hippocampal processing during rearing is also supported by the functional recordings 24 . Barth et al. 24 analyzed hippocampal current source density profiles during rearing and showed that rearing was accompanied by a prominent sink in the dentate gyrus and increased theta-gamma coupling in the terminal fields of the perforant path that originated in the medial entorhinal cortex 24 . Finally, molecular work in the dentate gyrus demonstrates a potential link between rearing, dentate synaptic plasticity, and spatial memory 59 .
The present work also does not offer new insight into the dissociation between 'supported' and 'unsupported' rearing. 'Supported' , and 'unsupported' refers to whether a rearing animal places its forelimbs on a surface while rearing to support itself. Prior work has shown these two forms of rearing to be dissociable. For example, selective breeding for unsupported rearing did not lead to a simultaneous increase in supported rearing 57 . Further, each supported and unsupported rearing are dissociable by their respective sensitivity to different motivating factors such as stress or motivation to escape 60,61 . The radial arm maze used in the present work, however, had narrow corridors, and there were few occasions when the rats did not place a fore paw on a wall. Thus, though not formally analyzed, we casually observed that virtually all rearing events would have been classified as supported rearing. Moreover, the closed loop inactivation protocol did not dissociate between unsupported and supported in any case. Thus, separate work is required to determine if different results would have been obtained if inactivation had been restricted to one type of rearing or the other. Sex differences have been reported in exploratory behaviors like rearing in a number of previous studies 15 , with females tending to rear more than males 62-69 . We did not find any gross differences in the average number of rears compared to the female and male rats used in the control group. This may be due to the lack of anxiety/novelty conditions present in prior studies because of the similar extensive experience on the task shared by each rat regardless of its individual sex.
Finally, regarding the specificity of our results, there are two sources of ambiguity worth noting. First, related to the discussion of supported versus unsupported rearing above, it may be that not every rearing event directly contributes to spatial memory encoding. Careful analysis of hippocampal dynamics across rearing events in future work could offer insight in this regard. Second, the current work does not address whether the specific form of encoding that occurs during rearing could happen in the absence of rearing. There are, for example, other behaviors that share a common phenotype with rearing that we call a 'attentive sampling from place. ' For example, Monaco et al. described 'lateral head-scanning' behaviors that were coincident with endogenous formation of new place fields in hippocampal CA1 neurons 70 . In both rearing and lateral head-scanning, animals stand in one location and actively orient towards distal cues. Functionally, both are characterized by high amplitude theta in the hippocampus 24,70 . However, there is evidence to suggest that the encoding that occurs during rearing may not readily occur during non-rearing behaviors. Mun et al. induced inflammation in a mouse's hind paw and found that it simultaneously hindered rearing and reduced sensitivity to spatial novelty 19 . Implicit to this result is that the mice did not switch to an alternate strategy to perform the same encoding when rearing was rendered uncomfortable. Thus, while the results of the present work are sufficient to demonstrate that rearing is an epoch of hippocampal dependent spatial memory encoding, additional work is needed to fully specify the relationship between rearing and spatial memory encoding.
Final summary and conclusion: this work demonstrates that disrupting activity in the dorsal hippocampus proper during rearing events is sufficient to disrupt spatial memory encoding in the delayed win-shift radial arm maze task. This result indicates that rearing events are an epoch of hippocampal dependent spatial memory encoding.

Data availability
The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request. www.nature.com/scientificreports/ Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http:// creat iveco mmons. org/ licen ses/ by/4. 0/.