Abstract
The combination of trimeric form of the light-harvesting complex II (LHCII3), a porous graphite electrode (GE), and the application of phenyl-p-benzoquinone (PPBQ), the quinone derivative, allow the construction of a new type of biohybrid photoactive system. The Chl fluorescence decay and voltammetric analyzes revealed that PPBQ impacts LHCII3 proportionally to accessible quenching sites and that PPBQ forms redox complexes with Chl in both ground and excited states. As a result, photocurrent generation is directly dependent on PPBQ-induced quenching of Chl fluorescence. Since PPBQ also undergoes photoactivation, the action of GE-LHCII3-PPBQ depends on the mutual coupling of LHCII3 and PPBQ photocycles. The GE-LHCII3-PPBQ generates a photocurrent of up to 4.5 µA and exhibits considerable stability during operation. The three-dimensional arrangement of graphite scraps in GE builds an active electrode surface and stabilizes LHCII3 in its native form in low-density multilayers. The results indicate the future usability of such designed photoactive device.
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Introduction
In plants, light energy is efficiently captured by antenna complexes (LHC) and then transferred to the reaction centers located at the core of photosynthetic complexes, Photosystem II (PSII) and Photosystem I (PSI), where charge separation occurs, and electrons transfer starts1. The main peripheral antenna of PSII, the light-harvesting complex II (LHCII), plays a crucial role not only in light harvesting processes, but also in the protection of chlorophyll-protein complexes (CP) by dissipating excess absorbed light energy2. LHCII exists in thylakoids in trimeric form (LHCII3) wherein every monomer is composed of Lhcb polypeptide, including three transmembrane α-helices, and precisely located in the protein scaffold the molecules of 8 chlorophylls a (Chl a), 6 chlorophylls b (Chl b), and 4 xanthophylls3.
Some research on artificial photosynthesis concerns biophotovoltaic devices, which are biohybrid systems connecting the CP with the electrodes. In these systems, mainly the PSII, PSI, or bacterial reaction center (BR) are used due to their efficient charge separation and generation of high potential difference between the electron donor and acceptor terminal sites4,5,6. The challenge is to integrate these CP complexes properly with an electrode, enabling unidirectional transfer of electrons and restriction of secondary redox reactions. Reducing the distance between CP and the electrode might be achieved by using a monolayer of complexes placed on a planar electrode surface7,8 or application of nanostructured material with a 3-dimensional (3D) porous structure9,10.
The electrodes might be built of Au or Ag-based materials or are made of electrically conductive metal oxides such as indium-tin oxide (ITO) or fluorine-doped tin oxide (FTO). If the surfaces of the ITO / FTO electrodes are coated with semiconductors (TiO2 or ZnO), the devices containing CP might be qualified as biological dye-sensitized solar cells (DSSC)6. Different carbon nanomaterials, among others graphite and graphene, can become the alternative material for the electrode of biohybrid systems due to their high electrical conductivity, broad range of electrochemical potential, and the possibility to design nanostructures with the increased surface area11,12,13,14.
The challenge, common to all biohybrid systems, is the efficient electron transfer between the complexes and the electrode. One of the solutions is direct electron transfer (DET) by coupling the CP with the electrode by various linking molecules. In mediated electron transfer (MET), diffusing redox reagents are chosen on the basis of their electrochemical potential. Furthermore, various systems combining DET, and MET have been applied8.
In thylakoid membranes, the plastoquinone-9 (PQ) molecule consisting of the benzoquinone ring with the polyisoprenoid chain acts as an electron acceptor from PSII15. Therefore, the various derivatives of benzoquinone are commonly used as redox mediators undergo reduction by oxidizing the PSII-bound semiquinone16,17 or directly from isolated BR18. However, these redox properties of quinones are not often utilized in biohybrid systems19,20. Independently of their participation in direct redox reactions, quinone derivatives, among others, phenyl-p-benzoquinone (PPBQ), specifically quench the excitation energy in BChl c antennas and isolated LHCII21,22. However, this property of quinones has not been tested in the performance of biohybrid systems yet.
The LHCII lacks the special Chls pair necessary for charge separation, and the light-harvested energy for further excitation energy transfer is directed to the so-called terminal emitter domain, including a cluster of coupled Chl a23,24. Thus, the LHCII in vivo is not the source of electron flow. Because of, these complexes have been used as a photosensitizing layer in the construction of DSSC, so far25,26,27,28,29. All the results obtained indicate that LHCII is not inferior to other CP complexes used in DSSC construction and can be used to design other biophotovoltaic devices. An additional advantage of LHCII is its smaller size and simpler structure comparing to those of PSI and PSII complexes, which increases its stability and facilitates the introduction of structural modifications.
We have previously found that the generation of photocurrent by LHCII immobilized within porous graphite electrode (GE) might depend on endogenous PQ molecules, non-specifically bound to complexes12. In this report, we verify the operation of a graphite electrode-based biohybrid system, in which we use LHCII3 as the photoactive layer and PPBQ as the MET reagent. The choice of synthetic quinone has relied on the properties of these compounds in quenching Chl fluorescence and electron transport capabilities in biophotovoltaic systems. Our results show that the combination of these properties enables the design of an efficient photocurrent generating system. Furthermore, our study shows that LHCII freely migrates into the interior of the porous GE, as well as that the PPBQ redox reaction proceeds more efficiently in the presence of immobilized LHCII3. We have based our conclusions on measurements of Chl fluorescence quenching, PPBQ light reduction, voltammetry, and electrochemical impedance spectroscopy as well as analysis by fluorescence microscopy in situ. Finally, our work highlights the ability to use LHCII in connection with quinone derivatives in a biophotovoltaic device based on the carbon electrode.
Results and discussion
Photocurrent generation and Chl fluorescence from GE-LHCII3 in the presence of 0.1 mM PPBQ were detected simultaneously under the 20 s pulse (Fig. 1A) and continuous illumination (Fig. 1B). The illumination of this GE-LHCII3-PPBQ system with the blue light of 4900 μE followed the 60 s dark period induced a rapid, in the 4 s range, increase of fluorescence emission at 680 nm (Fig. 1A,B). At the same time, in the 2 s range, the anodic photocurrent was growing to maximum levels in both experimental conditions (Fig. 1C,D). Although both processes underwent limited quenching (Fig. S2), the maxima of anodic photocurrent were invariable under the nine dark/light cycles and, except for the 60 s declining period, under continuous illumination (Fig. 1D). This quenching phenomena is probably related to transient processes in the diffusion layer at the GE surface30.The Chl fluorescence, but not the photocurrent, completely declined when the light was turned off. Beginning from the dark period, the opposite cathodic current was noted (Fig. 1C,D), which in the minimum point abruptly changed the direction towards the anodic one and hyperbolically increased up to the initial value (Fig. 1C,D). Although photocurrent was generated in the GE-LHCII system without exogenous redox mediators12, the addition of the PPBQ to the reaction buffer caused a 20-fold increase in photocurrent intensity (Fig. S3A), whereas the release of PPBQ from the reaction vessel decreased this reaction (Fig. S3B).
We have investigated the generation of current in the function of light intensity for two experimental conditions, (i) the system contained LHCII3 and PPBQ (GE-LHCII3-PPBQ) and (ii) solely for redox mediator (GE-PPBQ) (Fig. 2). The illumination of GE-LHCII3-PPBQ with gradually increasing photon flux density (PFD) of actinic white light caused the near-linear increase of the anodic photocurrent up to about 4.5 μA/cm2 (Fig. 2A,B). Arising simultaneously dark current revealed a relatively low amplitude and was dependent on light intensity (Fig. 2A). The illumination of GE-PPBQ with the gradually increasing intensity of white light caused a rapid generation of the cathodic current, which revealed the hyperbolic relation to light intensity (Fig. 2C,D). However, the light-dependent flow of current in the GE-PPBQ system was more complicated. The cathodic current after 4–6 s rapidly changed the direction towards the anodic one (Fig. 2C). The successive dark/light cycle caused an increase of the overall electrochemical potential of the GE-PPBQ system, what can suggest a gradual shift in the ratio of various radical forms of PPBQ, with different redox properties, as described for other quinone molecules31. In the GE-LHCII3-PPBQ system, the course of Chl fluorescence was linear in relation to PFD (Fig. 2E) and exponential concerning photocurrent generation (Fig. 2F), indicating that at higher PFD relatively more absorbed energy is dissipated than converted to the photocurrent.
Analyses of light-dependent Chl fluorescence quenching in the presence of PPBQ were performed under two experimental conditions, using the LHCII3 solution (Fig. 3A–D) and the GE-LHCII3 system (Fig. 3E–H). The overall Chl a fluorescence of LHCII3 in the absence of PPBQ decreased proportionally to PFD (Fig. 3B), and the initial velocity of quenching (Vi) increased with rising light intensity (Fig. 3C). Otherwise, the overall decline of fluorescence in the presence of 20 µM PPBQ was independent of light intensity (Fig. 3B). However, the initial quenching velocity in the presence of PPBQ (Vi+PPBQ) decreased proportionally to PFD and revealed the opposite direction to Vi (Fig. 3C). It should be noted that the sum of Vi and Vi+PPBQ for successive points of light intensities was practically invariable taking roughly 0.0084 fluorescence unit s–1, suggesting that the total velocity of fluorescence quenching, induced by light and PPBQ, is impassable at a particular concentration of LHCII3. The quenching of fluorescence measured at stable illumination (21 µE) and increasing concentration of PPBQ reached saturation at its concentration slightly above 20 µM (Fig. 3D). These data imply that the PPBQ is an efficient excitation quencher which impacts proportionally to the number of accessible quenching sites in LHCII3, what is in agreement with previous observations22.
The decline of fluorescence of GE-LHCII3 under continuous illumination was similar to the quenching detected for LHCII3 solution at the same PFD (Fig. 3F), whereas the fluorescence quenching observed for dark/light cycles was about one-half smaller, probably due to partial reversal of light-related quenching effect after each period of darkness (Fig. 3E)32. The addition of 0.1 mM PPBQ to the GE-LHCII3 system decreased the initial fluorescence levels by about one-half, but the subsequent illumination deepens the quenching effect only by about 20% (Fig. 3G,H). These indicate that the total fluorescence quenching of GE-LHCII3 is dependent on the combined effect of PPBQ concentration, light intensity, and accessible quenching sites in LHCII3. Furthermore, a comparison of fluorescence quenching of LHCII3 solution (Fig. 3B) and GE-LHCII3 (Fig. 3H) indicates that the concentration of 0.1 mM PPBQ applied for the generation of photocurrent (Fig. 1,2) was used above the saturation.
The PPBQ-dependent fluorescence quenching of LHCII3, as well as participation in energy transfer from LHCII3 toward GE, require close contact between PPBQ and LHCII3. Because this interaction could influence the electrochemical properties of the biohybrid system, we performed series of voltammetric analyses. The differential pulse voltammetry (DPV) analyzes showed that the oxidation and reduction peaks for PPBQ were significantly far away from peaks for LHCII3 (around + 0.500 V and − 1.200 V) (Fig. S4).
The DPV analyzes of PPBQ in a narrower potential range under dark conditions revealed a small anodic peak I at around − 0.260 V and a higher peak II at roughly + 0.055 V (Fig. 4A). The sample illumination caused the appearance of an additional peak III around + 0.460 V (Fig. 4A). The statistical analyses (Table 1) showed that the potential of peak I is not dependent on light conditions, whereas the potential of peak II significantly shifted towards higher values under illumination (+ 0.074 V). Otherwise, the cathodic peak IV (− 0.180 V) shifted to more positive values (− 0.160 V) (Fig. 4B, Table 1). Similar data were obtained using cyclic voltammetry (CV) (Fig. S5B) and square wave voltammetry (SWV) (Fig. S6A,B, Table S1). The absorption spectra of the illuminated PPBQ solution also showed the formation of new reduced forms of this compound (Fig. S5A).
The DPV scans of GE-LHCII3-PPBQ under dark conditions revealed the dominated anodic peak II at roughly + 0.120 V and cathodic peak IV (− 0.210 V) (Fig. 4C,D, Table 1). The light conditions induced the appearance of the additional anodic peaks I and III (− 0.270 and + 0.490 V) on the DPV scans, whereas the reduction peak IV was like that detected under the dark conditions (Fig. 4C,D, Table 1). The main voltammetric differences between GE-PPBQ and GE-LHCII3-PPBQ ones were presented in DPV as well as in SWV scans as a shift of the maximum of the peak II by about 0.060 V toward more positive potential (Figs. 4. Fig. S6, Table 1). Significant potential shifts were also shown for anodic peak III and cathodic peak IV, toward the more positive or more negative values (Table 1), respectively. These changes were observed both for dark and light conditions.
The data presented above proved that the specific interaction between PPBQ and LHCII3 is necessary to initiate the anodic current flow (Figs. 1, 2, 3, 4). Simultaneously, we found that in the GE-PPBQ system the illumination induced a cathodic photocurrent, which changed to the anodic in a few seconds (Fig. 2C). Unexpectedly, this phenomenon is observed under a dark gap during photocurrent generation in the GE-LHCII3-PPBQ system (Fig. 1C). Analyzes of absorbance spectra during photochemical reactions revealed that light-induced reduction of PPBQ occurred in the buffer phase independently on photocurrent generation (Fig. S7) and that the alternating photocurrent flow is likely related to the rate of diffusion of reduced forms of PPBQ into GE (Fig. S7I). Quinones undergo redox processes including reactions of single electron transfer, proton-coupled electron transfer and hydride transfer. These lead to the formation of a number of molecular forms of quinones, as well as radical intermediate compounds. Quinone photoactivation changes their redox potentials, improves their ability to accept hydrogen, and increases their reactivity31,33. Thus, observed light-induced shift of the PPBQ redox potentials (Figs. 4, S6, Table 1) indicate the formation of PPBQ forms with higher reactivity, and only these forms take up or give up electrons to the GE (Figs. 2C, S7I).
The quenching property of PPBQ was confirmed in our study both in solution and in GE-LHCII3-PPBQ system (Fig. 3). The quencher can interact with Chl molecule through a dynamic or static mechanism. The first one occurs through collisions with excited Chl molecules, whereas in the second one the quencher forms a complex with a Chl in the ground state22. Our voltammetric analysis of GE-LHCII3-PPBQ system revealed the significant shift of redox potentials regarding to GE-PPBQ both under dark and light conditions (Figs. 4, S6, Table 1), which can indicate the formation of complexes between PPBQ and Chl in ground as well as in excited states. This assumption can be substantiated by the previously observed ground-state interactions between chlorophyll analogue molecule, zinc chlorin ZnCe6 and benzoquinone, where Zn ion bound in the Ce6 moiety was required for complex formation34. Furthermore, the quenching effects of quinones on excited states of ZnCe6 were analyzed in solution as well as with use of peptide maquette, where peptide scaffold ensures stabilization of the pigment and therefore higher quenching efficiency34,35,36. The proposed quenching scheme assumes that formation of the coupled radical pair of the pigment cation and quinone anion (P·+Q·−) by charge-transfer reaction leads to quenching of the excited pigment P*. In the next step, the radical pair can be separated into pigment radical and quinone radical (P·+ + Q·−), what makes possible further electron transfer reactions to other molecules by charge separation reactions34.
Questions can be raised whether a similar phenomenon could occur in our experimental GE-LHCII3-PPBQ system, namely (i) formation of P·+Q·− radical pairs and (ii) the further electron transfer by the charge separation mechanism leading to photocurrent generation. (Figs. 1, 2). The Chls and carotenoids in LHCII are precisely oriented to maximize light-harvesting efficiency and transfer energies to a Chl a in terminal emitter domain of Chls 610/611/612 or to Chl a 604, the alternative energy sink23,24. Considering that these domains might be sites of the efficient energy transfer to quinones, the theoretical possibility of specific binding of PPBQ to Lhcb1 protein was checked. Our computer models obtained with ROSIE software indicated the plausible binding of PPBQ with Lhcb1 protein in the domains corresponding to the sites of binding of these terminal Chls (Fig. S8C,D), which makes the formation of a Chl-PPBQ complex probable.
The currently proposed model for dissipation of excited state in isolated LHCII assumes that this process is located in domain of Chls 610/611/612 along with its associated conjugated amino acids (aa) residues. This model predicts the formation of Chl–Chl charge-transfer states as quenching intermediates and sequence of proton-coupled electron transfer steps involving associated aa37,38. Our computer model (Fig. S8C) indicates that PPBQ can be bound by 178–183 aa residues, close to this quenching domain. Considering the efficient quenching properties of PPBQ (Fig. 3), voltammetric data (Fig. 4) and structural model, it is very likely that redox complexes arise in the terminal emitter domain of LHCII. Since PPBQ can bind to aa residues involved in quenching process37, it is possible that these aa residues involved in the formation of redox-active complexes. The potential of the exciting quinones undergoes changes due to the ground state, what causes easier reduction or oxidation those molecules18,33. Thus, in the case of PPBQ, the light-induced quinone forms (Fig. 4, Table S1) might be active components of the quenching state, created directly or indirectly with excited Chl.
The PQ are specifically bound to domains inside the PSII protein scaffold and the polarity of aa residues surrounding bounded mediator supports the redox properties of quinones and favors further electron transfer16. In the case of PPBQ and LHCII3 interactions, our computer model shows that PPBQ is bound more superficially to the protein and exposed to aqueous environment (Fig. S8). In aqueous solution, the equilibrium between Q·− and conjugated semiquinone radical HQ· is established, which may prevent the feedback reaction34. Thus, it is possible that the PPBQ radical, formed as a result of Chl quenching (Fig. 4), interacts with the water molecules and undergoes protonation, which may favor charge separation in LHCII3-PPBQ system. At the PSII core complex, the electrons from the reaction center are transferred to PQ bounding in QB site, where the stable semiquinone radical \({\mathrm{Q}}_{\mathrm{B}}^{\bullet -}\) is formed, which undergoes protonation to QBH2. The diffusion of QBH2 from the QB site is thermodynamically favorable, providing efficient exchange of the PQ/PQH2 pool39. It can be assumed that the protonated quinone could diffuse toward the GE in the system of LHCII-PPBQ, starting the current flow (Figs. 1, 2).
However, we found the formation of Chl-PPBQ redox complexes in the ground as well as in the excited state (Fig. 4, Table 1), but the duration time of these complexes was not determined. Thus, it cannot be excluded that these complexes are stable and that there is no diffusion of a protonated form of PPBQ. Simultaneously, the light induced formation of excited/reduced forms of PPBQ in buffer phase (Figs. 4, 5) and LHCII-independent current flow occurs (Figs. 2, 5). Besides that, the short-lived cathodic dark-current is probably related to the establishment of equilibrium between the different redox forms of PPBQ (Figs. 1, S7). This suggests that PPBQ reveals dual action, while some molecules bind to the LHCII3, and form complexes involved in quenching reactions, and others transfer the charge and enable photocurrent to appear. Since the value of the photocurrent generated by the GE-PPBQ system is ten times smaller than that by the GE-LHCII3-PPBQ (Fig. 2), there must be a coupling point between the bound and free PPBQ that allows efficient transfer of the light energy harvested by LHCII3. However, the interaction of Chl-PPBQ complexes with the electrode by the DET mechanism cannot be dismissed either. The exact solution to these issues will be possible after applying time-resolved measurement methods enabling to investigate the excited states of Chl and quinones18,34,37. Regardless of the need for a detailed explanation of the system's operation, our GE-LHCII3-PPBQ system efficiently generates a photocurrent proportional to the light intensity up to the value of about 5 μA/cm2 (Figs. 1, 2). The internal quantum efficiency (IQE) calculated for blue light as was described in40 and estimated to 0.66% indicates an efficient charge separation, also compared to other biohybrid systems40,41.
Regardless of charge separation efficiency, the effectiveness of the biohybrid system is attributed to active electrode surface area, which affects both the DET and MET response7,8,9,10. Therefore, we checked the electrochemical processes occurring in the vicinity of the GE by Electrochemical Impedance Spectroscopy (EIS). At the applied potential, no redox processes were observed on the empty GE immersed in the buffer and on the GE covered by LHCII3 in glutaraldehyde matrix (GE-LHCII3), as presented by no semicircle part in the Nyquist plots and no peak in the Bode phase plots (Figs. 5A, S9C). The relatively low double-layer charging (Table 2) can prove the LHCII3 migration into pores of the graphite electrode and its electrostatic stabilization. In the presence of PPBQ the semicircles at the Nyquist plots (Fig. 5B) and peaks at Bode phase plots (Fig. S9B,D) appeared. The values of double-layer charging increased more than 10 times for GE-PPBQ and more than 100 times for GE-LHCII3-PPBQ comparing to systems without PPBQ (Table 2). Furthermore, a lower charge transfer resistance was observed in the GE-LHCII3-PPBQ than in the GE-PPBQ (Fig. 5B vs. A and Table 2). This means that in the presence of LHCII3, PPBQ underwent a redox reaction more easily, so in GE-LHCII3-PPBQ the electron transfer was more efficient, and the conductivity improved. Furthermore, the linear part of the Nyquist plot for GE-LHCII3-PPBQ indicated that in the presence of LHCII3 increased diffusion of PPBQ between the electrical double layer and the buffer phase (Fig. 6B) occurred.
Independently on electrochemical methods, the properties of the electrode surface were directly investigated by confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM). Using the CLSM and a special holder (Scheme S2), we were able to obtain successive optical cross sections of GE-LHCII3-PPBQ and determine changes in the three-dimensional (3D) position of the LHCII3 inside GE during in situ experiments. LHCII3 deposited on GE surface before electrochemical treatment was shown as flat, partially undulated layers with 3–6 μm thickness (Fig. 6A,B), suggesting that the LHCII surface covering the GE was composed of many layers, where neighboring LHCII3 are located close to each other. Imaging of Chl fluorescence from the same area after 20 min of OCP treatment under the dark conditions revealed that the density of fluorescence in the single optical layer decreased, and fluorescence was recorded in a larger number of layers localized deeper in the GE structure (Fig. 6C). The 3D models indicated that the changes in fluorescence localization occurred in both vertical and horizontal directions (Fig. 6B versus D).
We are no longer dealing with the flat multilayer surface, but with the irregular 3D structure with 16–22 μm thickness (Fig. 6D). The total surface area (A, μm2) and volume (V, μm3) of 3D models were roughly 5 and 9 times higher, respectively, after OCP treatment and the A/V ratio decreased from 3.6 to 2.0, which is typical for the increasing volume of ellipsoid-like structures. Observed by CLSM in situ migration of LHCII3 agreed with SEM images, where the disappearance of the surface layer of LHCII3 after OCP treatment was observed (Fig. S10C). The fluorescence spectra recorded in different depths of GE-LHCII3-PPBQ revealed the same shape with a maximum of around 684 nm (Fig. S11), similarly to room temperature spectra of isolated LHCII42. The signal of free Chl at 665 nm was not noted12, that confirms retaining of LHCII3 in its native structure. These observations point to quite unlimited migration of LHCII3 into GE with concomitant decreasing of surface-density of complexes.
The LHCII was so far applied in the construction of DSSC as a photosensitizing layer on TiO2/FTO surface with the use of \({\mathrm{I}}_{3}^{-}\)/3 \({\mathrm{I}}^{-}\) as a MET reagent. The operation of such systems assumes that the excited state of Chl introduces electrons into the conduction band of TiO2, while the operation of the \({\mathrm{I}}_{3}^{-}\)/3 \({\mathrm{I}}^{-}\) cycle fills the electron gap created by charge separation in the Chl molecule. These systems have revealed high stability and long operation times. However, the charge regeneration occurs with low efficiency and the mechanism of this process has been not explained yet26,27,28. Our biohybrid system operates by a different mechanism based on the effect of quenching of excited states of Chl combined with the MET mechanism, both of which depend on the presence of exogenous quinone.
The choice of graphite as a solid phase was dictated not only by its electrochemical properties and porous structure but also by the prospects associated with the development of carbon nanomaterials. Diffusion of LHCII3 into porous GE leads to a reduction in the density of the complexes (Fig. 6) and decreases the probability of excitation quenching by complex aggregation22. The relatively low charge transfer resistance for GE-LHCII3-PPBQ suggests that LHCII3 did not form tight multilayers at the electrode surface (Table 2). The redox reactions involving PPBQ as well as diffusion of PPBQ increased in the presence of LHCII3 (Fig. 5, Table 2), which would support the assumption of a PPBQ pool linking quenching reactions with electron transfer toward GE. Both microscopic and EIS analyses suggest bounding of LHCII3 to exposed surfaces of the graphite scraps, which leads to the formation of a large 3D active surface area.
Conclusions
The combination of three elements (i) the trimeric (LHCII3) antenna complexes, (ii) the porous graphite electrode (GE) and (iii) the application of PPBQ, the quinone derivative, which acts as a quencher of excited Chl in LHCII3 and as an electrochemical mediator, allows the construction of a new type of biohybrid photoactive system (GE-LHCII3-PPBQ). This system has generated current proportional to a wide range of light intensity and has shown an internal quantum efficiency of at least 0.66%. The three-dimensional arrangement of the graphite scraps in GE ensured that LHCII3 was bound throughout the electrode volume and stabilized in its native form on graphite surface probably in low-density multilayers. The primary excitation quenching reaction by PPBQ is associated with the formation of Chl-PPBQ redox complexes, both in the ground and excited states. The inference is complicated by the fact that PPBQ is a photoactive chromophore independently generating photocurrent. Thus, it can be assumed that coupling of LHCII3 and PPBQ photocycles causes a rise of photocurrent generation in GE-LHCII3-PPBQ system. Our research indicates the possibility of using antenna complexes in connection with quinone derivatives and carbon electrode in construction of useful biophotovoltaic device. Further research on the use of quinone derivatives with higher affinity to LHCII and carbon materials enabling enlargement of the active surface of the electrode should lead to increased performance of this biohybrid system.
Methods
Isolation of LHCII complexes
LHCII was isolated from spinach leaves purchased at the local market according to the protocol described previously43. The isolated LHCII complexes were suspended in 20 mM Tricine buffer (pH 7.8) and stored at – 80 °C until use. The purity of the preparation has been tested using SDS-PAGE electrophoresis and immunodetection. The analyzes showed the presence of Lhcb1, Lhcb2, and Lhcb3 polypeptides and the lack of the D1 and D2 of PSII core subunits (Fig. S1), which indicates the high purity of the LHCII samples used according to our previous results 12.
Screen-printed graphite electrode (GE) and pretreatment procedure
All electrochemical experiments were carried out using a disposable planar screen-printed three-electrode system composed of a round graphite working electrode (geometric area of 0.07 cm2), an Ag/AgCl pseudo-reference electrode (Ag/AgCl RE), and a graphite counter electrode. The properties of this graphite electrode (GE), as well as the procedure of cleaning and activation of the carbon surface, were described in details previously12,44. The potential of a pseudo-reference electrode in HEPES buffer (20 mM HEPES, 15 mM NaCl, 4 mM MgCl2, pH 7.5) was controlled against the saturated calomel electrode (SCE) and was equal to 0.080 ± 0.005 V. This gives a potential equal to 0.324 ± 0.005 V against a normal hydrogen electrode (NHE). All potentials reported in this study are given in comparison to the Ag/AgCl RE.
LHCII trimers (LHCII3) and GE-LHCII3 preparation
A trimeric form of LHCII was formed by the addition of n-Dodecyl-β-D-maltoside (DDM) to freshly defrosted LHCII, at a final concentration of 0.025% (w/v). The trimeric state was confirmed by analyses of Chl fluorescence spectra at 77 K (Fig. S1) showing the typical fluorescence emission bands45. The LHCII3 was mixed with glutaraldehyde to the final concentration of 0.2 µg Chl/µl and 0.75% (w/v) glutaraldehyde and 1.5 µl of the final suspension was immediately deposited on the graphite surface of the working electrode (GE) and followed dried at room temperature for 15 min to form a layer of LHCII3-glutaraldehyde. Then, after immersion in HEPES buffer and determination of the open circuit potential (OCP), the electrode was exposed to this potential for 1 min, what finally resulted in forming GE-LHCII3 biohybrid system. The LHCII3 preparation procedures were performed under diffuse green light whereas OCP values were determined in the dark.
The electrochemical and fluorescence measuring system
A holder, designed especially for our experiments, enables placement of a glass cuvette within GE in a closed box with three sockets connected by the optical fibers with the actinic light source and spectrofluorimeter (Schematic diagram S1). The GE was connected with AUTOLAB PGSTAT 128 electrochemical analysis system equipped with NOVA 2.1 software package (EcoChemie, The Netherlands). The measuring part of GE was immersed in HEPES buffer saturated with argon. Actinic light was achieved with the Schott KL 2500 LCD light source with an intensity from 950 to 29 200 µE. The photosynthetic photon flux density (given as µmol photons s–1 m–2; µE) was measured using a Universal Light Meter (ULM-500, Heinz Walz GmbH, Effeltrich, Germany).
For simultaneous detection of Chl fluorescence and photocurrent response, the fluorescence of the GE-LHCII3 in the presence of PPBQ was measured with the Shimadzu RF-5301PC spectrofluorimeter with 5 nm spectral resolution for excitation at 470 nm and emission at 680 nm. The actinic blue light (377–504 nm) was used, and the emission was recorded through LP600 filter to remove the influence of actinic light. The actinic white light (280–800 nm) was applied to induce the photocurrent itself, due to overlapping the red regions of spectra of actinic light and Chl a fluorescence emission.
The OCP established to roughly –0.150 V was applied in all experiments. Cyclic voltammetry (CV) was performed in potentiostatic mode, and the CV curves were recorded at the scan rate of 0.1 V s–1. Differential pulse voltammetry (DPV) and square-wave voltammetry (SWV) were performed under illumination and under dark conditions within the scan range from 0.3 to – 1.5 V and from – 0.7 to 1.0 V (versus Ag/AgCl) to monitor reduction and oxidation processes, respectively. Electrochemical impedance spectroscopy (EIS) was performed at a frequency range from 105 to 10–3 Hz to measure the internal resistance. Nyquist graphs were plotted using NOVA software. EIS measurements were conducted in the darkness in a homemade Faraday cage.
Chl a fluorescence and spectroscopy measurements
Chlorophyll a fluorescence quenching was measured in a solution of LHCII3 in the presence or absence of PPBQ by the Pulse-Amplitude-Modulation approach using the Dual-PAM 100 (Heinz Walz GmbH, Effeltrich, Germany). The excited modulated blue light (460 nm) with intensity below 2 μE and detection above 700 nm (long-pass RG9 filter) was applied to establish initial measuring conditions. Fluorescence quenching was induced by red actinic light (635 nm) with various intensities. The reaction buffer contained 0.025% (w/v) DDM to achieve the trimeric form of LHCII. The light-induced change in absorbance of LHCII3 or PPBQ solution was detected using Cary 50 Bio spectrophotometer (Varian Inc., Australia) in the range from 250 to 800 nm.
Confocal laser scanning microscopy (CLSM)
In situ experiments on LHCII3 migration to GE were performed with the use of the specially designated microscopic holder (Schematic diagram S2). CLSM images of the GE-LHCII3-PPBQ were made in a Nikon A1R MP inverted microscope equipped with galvo scanners and a Nikon S Plan Fluor ELWD 40 × Ph2 ADM lens. The final resolution of the image was set at 2048 × 2048 pixels (0.08 μm/pixel) in lateral dimensions and 1 μm in vertical dimensions. Fluorescence excitation was applied with a 488 nm diode laser, and emission was detected in the 570–620 nm wavelength range. Data stacks were deconvoluted using AutoQuant X3.0.5 software (Media Cybernetics Inc. MD, USA) and the three-dimensional fluorescence surface models were created using Imaris 8.4.0 software (Bitplane AG, Switzerland).
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Acknowledgements
The project was supported by the National Science Centre (NCN), Poland, decision number DEC-2013/09/B/NZ1/01111. We acknowledge Dr. Elżbieta Zwierkowska (Institute of Electronic Materials Technology, ITME) for providing the GEs. The best grateful to Jan Garstka (design cooperative TAKŁADNIE; https://takladnie.com, j.garstka@takladnie.com) for design and implementation of the special holders for simultaneous detection of the photocurrent and Chl fluorescence as well as for in situ imaging of the GE-LHCII3-PPBQ- system.
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M.G., M.Ł. provided conception of the project; M.Ł., M.G., A.P-S., M.M-Ż., B.P., R.M. designed the experiments and analyzed the data; P.P. performed the preliminary experiments introduced the use PPBQ as MET; M.Ł. performed the all presented experiments; M.G., M.Ł., A.P-S., M.M-Ż. wrote the manuscript; all authors read, corrected, and approved the final version of manuscript.
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Łazicka, M., Palińska-Saadi, A., Piotrowska, P. et al. The coupled photocycle of phenyl-p-benzoquinone and Light-Harvesting Complex II (LHCII) within the biohybrid system. Sci Rep 12, 12771 (2022). https://doi.org/10.1038/s41598-022-16892-y
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DOI: https://doi.org/10.1038/s41598-022-16892-y
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