Author Correction: Salidroside promotes peripheral nerve regeneration based on tissue engineering strategy using Schwann cells and PLGA: in vitro and in vivo

Correction to: Scientific Reports https://doi.org/10.1038/srep39869, published online 05 January 2017

This Article contains errors, that were not addressed in the previous correction¹.

In Figure 1E, the images for TCP at 2d, PLGA+S-0.1 at 4d, and TCP at 4d are incorrect. A corrected version of Figure 1 and its accompanying legend appear below.

Figure 1

Effects of SDS on RSC 96 via MTT analysis in vitro. (A) Chemical structure of salidroside; (B) Preliminary drug screening analysis of RSC 96 treated on PLGA scaffold with different concentrations of salidroside after 3 days (n = 3, mean ± SEM); (C) Proliferative effects of salidroside on RSC96 on PLGA scaffold measured by MTT assay (n = 3, mean ± SEM). Different letters denote significances with P < 0.05 and the same letter shows no significant differences (P ≥ 0.05); (D) Quantitative data of the mean number of SCs. Data of each bar are shown as the mean of three independent experiments ± SD. Different letters denote significances with P < 0.05 and the same letter shows no significant differences (P ≥ 0.05); (E) Cell viability was measured by FDA/PI staining under microscope. (PLGA means cultured with 0 mM SDS, PLGA+S-0.1 means cultured with 0.1 mM SDS, PLGA+S-0.2 means cultured with 0.2 mM SDS, PLGA+S-0.4 means cultured with 0.4 mM SDS, TCP means cultured on TCP alone, TCP + s-0.2 means cultured with 0.2 mM SDS on TCP).

In Figure 3, the images for PLGA+S-0.1 at 4d and PLGA+S.02 at 4d in Figure 3A and TCP at 4d in Figure 3B are incorrect. A corrected version of Figure 3 and its accompanying legend appear below.

Figure 3

Effects of SDS on RSC 96 via SEM, immunohistochemical analysis, Western Blot assay and gene expression analysis in vitro. (A) Scanning electron microscopy (SEM) of the cells on scaffolds at 4 days. (PLGA means cultured with 0 mM SDS, PLGA+S-0.1 means cultured with 0.1 mM SDS, PLGA+S-0.2 means cultured with 0.2 mM SDS, PLGA+S-0.4 means cultured with 0.4 mM SDS, TCP means cultured on TCP alone, TCP + s-0.2 means cultured with 0.2 mM SDS on TCP). Statistic analysis of scanning electron microscopy (SEM). Different letters denote significances with P < 0.05 and the same letter shows no significant differences (P ≥ 0.05). (B) Immunohistochemical analysis for S-100 protein, RSC 96 showed positive cytoplasmic staining for S-100 at 4 days. (PLGA means cultured with 0 mM SDS, PLGA+S-0.1 means cultured with 0.1 mM SDS, PLGA+S-0.2 means cultured with 0.2 mM SDS, PLGA+S-0.4 means cultured with 0.4 mM SDS, TCP means cultured on TCP alone, TCP + s-0.2 means cultured with 0.2 mM SDS on TCP). Statistic analysis of immunohistochemical analysis. Different letters denote significances with P < 0.05 and the same letter shows no significant differences (P ≥ 0.05). (C) Western Blot assay of S-100 protein and quantification of the proteins expression. Full-length blots are presented in supplementary information. Different letters denote significances with P < 0.05 and the same letter shows no significant differences (P ≥ 0.05); (D) Gene expression analysis of important neurotrophic factors (GDNF, BDNF and CNTF) by qRT-PCR in six groups at 2, 4 and 6 days. The gene expression levels were analyzed by the 2^−ΔΔCT method using GAPDH as the internal control. The data represent the mean of three independent experiments (n = 3, mean ± SEM). Different letters denote significances with P < 0.05 and the same letter shows no significant differences (P ≥ 0.05). (PLGA means cultured with 0 mM SDS, PLGA+S-0.1 means cultured with 0.1 mM SDS, PLGA+S-0.2 means cultured with 0.2 mM SDS, PLGA+S-0.4 means cultured with 0.4 mM SDS, TCP means cultured on TCP alone, TCP + s-0.2 means cultured with 0.2 mM SDS on TCP).