Group VR experiences can produce ego attenuation and connectedness comparable to psychedelics

With a growing body of research highlighting the therapeutic potential of experiential phenomenology which diminishes egoic identity and increases one’s sense of connectedness, there is significant interest in how to elicit such ‘self-transcendent experiences’ (STEs) in laboratory contexts. Psychedelic drugs (YDs) have proven particularly effective in this respect, producing subjective phenomenology which reliably elicits intense STEs. With virtual reality (VR) emerging as a powerful tool for constructing new perceptual environments, we describe a VR framework called ‘Isness-distributed’ (Isness-D) which harnesses the unique affordances of distributed multi-person VR to blur conventional self-other boundaries. Within Isness-D, groups of participants co-habit a shared virtual space, collectively experiencing their bodies as luminous energetic essences with diffuse spatial boundaries. It enables moments of ‘energetic coalescence’, a new class of embodied intersubjective experience where bodies can fluidly merge, enabling participants to include multiple others within their self-representation. To evaluate Isness-D, we adopted a citizen science approach, coordinating an international network of Isness-D 'nodes'. We analyzed the results (N = 58) using 4 different self-report scales previously applied to analyze subjective YD phenomenology (the inclusion of community in self scale, ego-dissolution inventory, communitas scale, and the MEQ30 mystical experience questionnaire). Despite the complexities associated with a distributed experiment like this, the Isness-D scores on all 4 scales were statistically indistinguishable from recently published YD studies, demonstrating that distributed VR can be used to design intersubjective STEs where people dissolve their sense of self in the connection to others.

Describing the sensations that arose immediately following a left-hemisphere stroke, brain scientist Jill Bolte Taylor recounted: I could no longer define the boundaries of my body. I can't define where I begin and where I end, because the atoms and molecules of my arm blend with the atoms and molecules of the wall, and all I could detect was this energy… I was immediately captivated by the magnificence of the energy around me. And because I could no longer identify the boundaries of my body, I felt enormous and expansive. I felt at one with all the energy that was, and it was beautiful 1 . Recounting the sensations that arose after seeing the Earth from space, astronaut Edgar Mitchell said: And suddenly I realized that the molecules of my body, the spacecraft, the body of my partners, were all manufactured in some ancient generation of stars. I felt an overwhelming sense of oneness, or connectedness… an insight, an epiphany 2 . During a particularly potent meditation session Zen master Sokei-an Sasaki described how: I lost the boundary of my physical body. I had my skin, of course, but I felt I was standing in the center of the cosmos… I saw people coming towards me, but all were… myself! 3 Recounting the sensations which arose from a high dose of the psychedelic drug (YD) psilocybin, a participant in a 2008 Griffiths 4 .
These accounts, which express a profound sense of unity with other beings and other objects, stand in contrast to our typical day-to-day perspective, in which we often default to conceptual representations of ourselves (and others) as separate objects rather than coupled interdependent subjects. Yaden  Combining the Greek words pneuma (πνευμα, 'breath' , 'spirit' , or 'soul') and delein (δηλεlν, 'to reveal' , 'to make visible' , or 'to manifest'), numadelic may be translated as 'spirit-manifesting' or 'spirit-revealing' . The numadelic aspect of Isness-C arises from the fact that participant bodies are represented as luminous energetic essences, an aesthetic representation associated with 'spirit' in a variety of wisdom and meditation traditions.
While Isness-C was (to the best of our knowledge) the first study using the MEQ30 to analyse subjective participant experience in a VR context, previous work has investigated the ability of both 2d and immersive video content to elicit two phenomenological ingredients known to be important components of a subjective YD-MTE, namely: (a) altered visual perception 29 , and (b) a sense of awe 23,30 . The former has been studied by Suzuki et al., who presented individuals with immersive 360 videos derived from Google's deep dream convolutional neural nets, rendered in a VR headset 31,32 . Their results (N = 12) suggested that it is possible to induce visual phenomenology similar to psilocybin; however, they were unable to evoke in participants the corresponding sense of temporal distortion that psilocybin produces. The latter-awe-has been the topic of several studies. Broadly, these studies have shown that awe can be reliably elicited by presenting individuals with video content of various environments, e.g.: views from a mountaintop, within a vast open space, seeing earth from space, submerged in the ocean, amongst very tall trees, etc [33][34][35][36][37][38] . Recent fMRI studies suggest that participants presented with awe-eliciting 2d videos have reduced activity in the brain's default mode network 39 , which is an ego-reaffirming neurological network that YDs also attenuate 15 . Chirico et al. showed that immersive videos rendered in headmounted-displays (HMDs) are able to elicit a stronger sense of awe compared to standard 2d videos 33 , given that VR enables a heightened sense of immersion 40,41 .
The two items which Yaden et al. identified as primary phenomenological ingredients of STEs-(a) a weakened sense of self-boundaries and diminished self-salience; and (b) a 'relational' sense of unity with something beyond the self-are not easy to untangle. The difficulty becomes apparent by undertaking a simple thought experiment, wherein we imagine the dynamics that arise when two drops of liquid water coalesce: their individual identities and associated properties fade away as their respective boundaries co-mingle and eventually dissolve into one another, eventually creating a drop with new physical properties. Characterizing the softness, fluidity, or porosity of a body's boundaries is impossible without corresponding observations of the dynamics that unfold when it undergoes some form of relational interaction with another body. The tight relational co-dependence of these phenomenological ingredients, in which one is required to characterize the other and vice-versa, suggests that they are perhaps best realized in a multi-body, relational context. This observation highlights a potentially significant gap in YD laboratory studies and efforts to design STEs using VR: nearly all of the work described to date involves individual, intrasubjective experiences.

Present work
In what follows, we describe our efforts designing intersubjective group STEs which enable participants distributed across the world (e.g., as shown in Figs. 2B and 3A) to collectively co-habit a shared virtual space and undergo an embodied experience of the overlap shown in the Inclusion of Other in the Self Scale in Fig. 1 (albeit in 4 dimensions (x, y, z, t) rather than 2 dimensions). Specifically, we constructed a multi-person cloud-mounted VR experience called Isness-distributed (Isness-D), which is illustrated in Fig. 3A,B. Harnessing the unique affordances of distributed multi-person VR, Isness-D's numadelic aesthetic blurs conventional boundaries between self and other. Isness-D participants are rendered as luminous energetic essences with radiance concentrated at the heart centre. Compared to the hard boundaries illustrated in the IOS Scale ( Fig. 1), Isness-D body boundaries are diffuse, fuzzy, and soft: they extend beyond the limits of the physical body, making it difficult to specify clearly where one body ends and another begins, as shown in Fig. 3B. This energetic aesthetic takes inspiration from subjective 1st person accounts of intense unitive experiences like those described at the beginning of this article, which often report a heightened sensitivity to a more fundamental energetic essence, and to the molecular and atomic constituents that combine to create our bodies and everything around us. The numadelic aesthetic is   42 . The embodied experience of energetic overlap, illustrated in Fig. 3A,B-where participants can fluidly merge their energetic virtual bodies with those of the others in the group-we refer to as 'energetic coalescence' . To the best of our knowledge, 'energetic coalescence' represents a new class of experience which is only possible within a multi-person distributed VR environment-i.e., it cannot be realized in any other way. This distinguishes Isness-D from previous work using VR to investigate STEs, which use VR to simulate STEs that are in principle available in the real world (YD visuals in the case of Suzuki, or awe experiences in the case of Chirico). In this respect, 'energetic coalescence' is similar to the 'body-transfer' work of Slater and co-workers (which enables a VR participant to inhabit the body of another person)-i.e., a 'body-transfer' experience is only possible using VR 43 . Whereas 'body-transfer' is an individual experience, 'energetic coalescence' is a group experience. The 'energetic coalescence' experience may represent a particularly explicit form of 'identity fusion' , which 'entails a visceral feeling of oneness with the group' 44 . As an experience of intense togetherness that temporarily transcends social structures, dissolving the norms that typically characterize relationships within structured institutional contexts, 'energetic coalescence' may represent an example of what Victor Turner called 'communitas' 45 . shows the international Isness 'node' locations as circular markers labelled with the total number of nodes in that location. There were 5 nodes in the San Francisco Bay Area (USA), 1 node in Tennessee (USA), 1 node in Massachusetts (USA), 6 nodes in Bristol (UK), and 1 node in London (UK). The Isness server was mounted on data centers D in London (UK), Frankfurt (Germany) and Washington D.C (USA). www.nature.com/scientificreports/ Isness-D is a group VR experience in which participants experience the emergence, dissipation, and fluctuation of their own bodies and the body of a simulated molecular 'organism' as energetic essences. Participants are guided by a combination of a live in-world facilitator and a pre-recorded narrative focused on the matterenergy relationship (which is described in further detail in the "Methods" (Sects. 6.8-6.9)). The software used to run Isness (described previously in ref 25 ) grew out of a multi-person room-scale VR framework 46,47 we initially designed to enable individuals [47][48][49] and then groups 47,[50][51][52] to cohabit virtual environments where they can reach out and sculpt the dynamics of simulated molecular objects [47][48][49] . Our work over the years suggests that people interacting with these simulations (who from the outside appear to be doing little more than waving their hands around in the air) are able to 'feel' differences in the simulated dynamical flexibility of different molecular objects 47,53 . This highlights a crucial affordance of VR: a modality that enables a tangible sensory experience of touching phenomena whose essences are purely energetic. For designing STEs, this is a particularly useful affordance, given that it is aligned with subjective YD accounts where participants report sensitivity to the energetic nature of their environment-famously encapsulated by Aldous Huxley's account of his mescaline experience. In a vase of flowers, he reported seeing "the miracle, moment by moment, of naked existence… flowers shining with their own inner light and all but quivering under the pressure of the significance with which they were charged… [even] the folds of my grey flannel trousers were charged with is-ness." 54 The presence of a molecular object whose dynamics were simulated in real-time was therefore an important intersubjective aspect of Isness-D. It represented for participants a kind of tangible, fluctuating organism (referred to at various points in the narrative as a 'molecular organism' or 'energetic thread') with a purely energetic essence, inviting people to recognize that their own essential energetic quality is shared with every other object (living and non-living) in the natural world. The depth of the Isness-D matter-energy narrative was reinforced by the fact that the dynamics of the molecular object were calculated in real-time using a state-of-the-art GPU-accelerated computational biophysics engine 55 . This sophistication anchored the Isness-D narrative in physical and scientific reality, encouraging participants to reflect on the fact that everyday material objects are actually constructed from the dynamical choreography of molecular organisms whose essences are fundamentally energetic 49 , an insight which arises in many first-person STE accounts (e.g., those at the beginning of this article). As shown in Fig. 3A.i, multiple participants rendered as energy bodies, connected across different locales and time zones via an energetic molecular thread, represents a class of experience that is only possible using the affordances of multi-person distributed VR.
Given that our laboratory (like many labs) was shut down as a result of COVID restrictions, we evaluated Isness-D using a 'citizen science' approach, coordinating a set of 14 "Isness-nodes" distributed across the world (Fig. 3C). Each node operated as a portal where a single participant could virtually enter into the shared Isness-D experience along with participants from other nodes. To analyse Isness-D, we again utilized the MEQ30 in order to facilitate direct comparisons with Isness-C. To assess other phenomenological ingredients known to be important during intense STEs, we used three additional measurement scales. First, we measured the extent to which participants in Isness-D experienced a unitive sense of connectedness with those joining from other Isness-D nodes. To measure this, we used an adapted version of the Inclusion of Community in Self (ICS) Scale, which is essentially a 5-item version of the IOS scale shown in Fig. 1, where participants were asked to rate the extent to which a circle representing the community of 'Other Isness Participants' overlapped with their sense of self 56 . Second, we measured the extent to which participants experienced a diminished sense of egoic identity, which (as discussed above) is closely interrelated with the intensity of the unitive experience, prominent within MTEs and YD experiences. To do this, we utilized the ego-dissolution inventory (EDI), which measures aspects of both ego dissolution and ego inflation, and has been utilized in prior YD studies [57][58][59][60] . Third, given that Isness-D was constructed as an intersubjective group experience, we attempted to measure the extent of 'communitas' , which has been defined as an experience of intense togetherness and shared humanity that temporarily transcends social structures, mediated by an anti-structural and often ritualized 'liminal' phase of equality amongst community members 45 . To do this, we used the recently validated psychedelic communitas scale from Kettner et al. 61 who showed that communitas experienced during intersubjective group YD experiences predicts enduring changes in psychological well-being and connectedness, with associated improvements in wellbeing, depressive symptoms, trait anxiety, and interpersonal tolerance.
Isness-D offers an interesting case study in developing technologies, methods, strategies, and protocols to carry out distributed multi-person studies in scenarios where face-to-face laboratory studies and clinical interactions are difficult. The lockdowns, social distancing, and uncertainty that have arisen as a result of the public response to the COVID-19 pandemic have created the conditions for increased social isolation and loneliness, which are correlated with feelings of fear, anxiety, stress, depressive thoughts, and helplessness [62][63][64] . While the longer term effects of COVID remain to be determined, past studies have highlighted social disconnectedness to be a key feature of depression 65 , while feelings of connectedness are associated with reduced depressive symptoms 66 . By encouraging participants to imagine their own bodies and those of others as energetic essences whose luminosity extends beyond the boundaries of their material form, Isness-D offers participants new experiences of connectedness, which may help address the isolation resulting from COVID. In this respect, experiences like Isness-D respond to recent calls to use human-computer interaction (HCI) technologies to design meaningful experiences 67 which deal with the fundamentals of human existence (e.g., mortality, identity, isolation, meaning) 68,69 in order to stimulate alternative narratives and visions within interconnected systems 70,71 and explore new forms of spiritual sensitivity 72,73 .

Results
Sample size, demographics, and emotional response. From Aug-Sept 2020, we carried out 29 Isness sessions distributed across our network of 14 Isness nodes, with 109 total participants. 24 participants were not included in the analysis because their participation formed part of a technical training session or sup- www.nature.com/scientificreports/ port role (i.e., as citizen science 'volunteers'). Nine participants were removed from the analysis because the facilitator or node host identified that their experience suffered significant technical difficulties-primarily glitches and dropouts as a result of network instability. This left 75 remaining participants whose Isness-D sessions could be analyzed. Of these, 54 answered the pre-session questionnaire, and 58 answered the post-session questionnaire. Of these 58 participants, 59% were male, 37% were female, 2% were other, and 2% declined to state. Ages ranged from 23 to 75 years old with a mean (SD) of 38 (10) years. Over half the participants (57%) had not been in VR at all over the last year. Four (7%) had used VR 20 + times in the last year. 12% had experienced the earlier, co-located version of Isness 25 . One reported a brief period of nausea (they did not comment on the strength or duration and finished their session as usual). Three reported body aches and three reported headaches. Six participants reported crying at some point during their Isness-D experience. Anecdotally, our study team obtained several other reports of people who experienced a brief period of tears. One participant reported trembling and two felt sweats or chills. None of the participants reported these emotional reactions to be negative or traumatic.

MEQ30.
The MEQ30 27 is a questionnaire that can distinguish dose dependent effects of YDs. It asks participants to rate the intensity with which they experienced 30 items on a 6-point scale [from "0 = none; not at all" to "5 = extreme (more than ever before in my life and stronger than 4)"], with three questions to capture ineffability I, fifteen mystical M [capturing unitive experiences, noetic quality, and sacredness], six positive mood P, and six transcendence of time/space T. Participant responses for each factor (I, M, P, T) are then averaged, and reported as a percentage of the maximum score. Figure 4 compares the Isness-D MEQ30 factor scores to 27 previous studies: 26 studies in the altered states database 28 , where the MEQ30 has been used to analyse YD altered states, and our previous Isness-C study 25 . To analyze these results, we followed the approach Barsuglia et al. 74 used to analyze MEQ30 results obtained during 5-MeO-DMT field tests. Specifically, we undertook comparative analysis of Isness-D to the previously published studies using independent sample t-tests with a significance level of 5% (α = 0.05) calculated using Python (see SM for further details). Our previous work has shown that independent sample t-tests give results broadly aligned with more sophisticated statistical analyses 25 . Figure 4 and Table SM2 show the results of 27 different independent-sample t-tests, comparing the Isness MEQ30 results to each of the studies in Table SM2. To make Fig. 4, we analyzed whether a study was statistically indistinguishable (p > 0.05) from Isness-D on each of its I, M, P or T factor scores 25 . Compared to Isness-D, Fig. 4 shows: Griffiths and co-workers identify an MEQ30 respondent as having had a 'complete MTE' when each of the I, M, P, T factor scores are ≥ 60% of the maximum 22 . In general, the fraction of participants reporting a complete MTE is proportional to the YD dose. Barrett and Griffiths 82 reported a meta-analysis of high dose (30 mg/70 kg) psilocybin studies in 119 healthy volunteers 13,14,75 , and observed that 57% of participants had 'complete' MTEs. Our analysis shows that 29% of Isness-D participants had a complete MTE, compared to the previous value of 44% for Isness-C.
Inclusion of community in self scale. 54 participants answered both the pre and post questionnaires, both of which included the aforementioned ICS scale shown in Fig. 5. The ICS asked which set of circles best describes their relationship with the other Isness participants from a series of images. The first image showed two circles with no overlap (a score of 0) and the last image showed two circles almost entirely overlapping (a score of 5). Figure 5 shows a significant shift in the distribution of ICS scores following Isness-D, with participants indicating higher degrees of connectedness. A Wilcoxon signed rank test showed that the difference between the pre and post values was statistically significant (p < 1E-6), with the mean ICS rating of 1.2 ± 1.5 (SD) pre-Isness-D increasing to 2.9 ± 1.4 (SD) post-Isness-D. The post-Isness-D ICS results are statistically indistinguishable from recent results published by Forstmann et al. 83 who investigated the effects of psychedelic substance use on positive mood and social connectedness of 450 participants (in naturalistic mass gathering settings) who used hallucinogens and/or euphorics in the last week. Given that the Forstmann et al. 83 scale ranged from 1 to 7, we mapped their results onto a 0 to 5 scale to enable comparison. Forstmann et al. found an average IOS of 2.8 ± 1.3 (SD), which is statistically indistinguishable from the post-Isness-D results according to independent sample t-tests (p = 0.510).

Ego-dissolution inventory (EDI).
The EDI 84 self-report scale is typically employed in psychedelic studies to measure subjective experiences of ego dissolution. It consists of 16 items: 8 relate to ego dissolution (e.g., I experienced a dissolution of my "self " or ego", I felt a sense of union with others, etc.) and 8 relate to ego inflation (e.g., I felt more important or special than others, I felt especially sure-of-myself, etc.). Following Isness-D, participants were asked to rate the extent to which each statement applied to their Isness-D experience on a scale of 0 (no, not more than usually) to 100 (yes, I experienced this completely). Cronbach's alpha was calculated, and the results showed high internal consistency for both ego dissolution (α = 0.86) and inflation (α = 0.87). Figure 6 shows that the ego inflation distributions are shifted to lower values compared to the ego dissolution distributions, with a mean ego dissolution across the cohort of 40 ± 20 (SD), and mean ego inflation of 17 ± 15 (SD). The results in Fig. 6 are consistent with subjective participant reports following LSD-equivalent doses of ~ 100 μg, based on inspection of values published by Nour et al. 84 Direct comparison of the complete Isness-D EDI scores to previous YD studies is complicated for the following reasons: (a) several previous studies use only a single EDI item to measure ego dissolution (usually I experienced a dissolution of my "self " or ego, or similar); and (b) for some of the previous studies where EDI was reported, patients were placed in an fMRI 57,58 or PET scanner 59 , which may lead to anxiety and impact the reported scores 85 . For Isness-D, the mean for this specific question was 43 ± 29 (SD), which is higher (p < 0.001) than the subjective responses following 75 µg doses of LSD published by Tagliazucchi et al. 57  Communitas. Following Kettner et al. 61 we used the scale in Fig. 7 to assess participants' Isness-D experiences. The first 8 items offer a subjective assessment of communitas, item 9 assesses participant-to-participant connection, and item 10 assesses participant-to-facilitator connection. The scale ranges from 1 (strongly disagree) to 7 (strongly agree). Calculation of Cronbach's coefficient showed high internal consistency within the 10 items (α = 0.84). A one-sample t-test against the midpoint of the scale showed that the overall mean of the 10 items across the participants was higher than the midpoint of the scale value (p < 0.001), indicating that a sense of togetherness was felt by participants during their shared Isness-D sessions. The item the ceremony really allowed me to get to know the other participants was the only one to receive a lower mean score than the midpoint scale value, perhaps because the Isness-D experience involved little verbal communication between participants. The rest of the items all received ratings higher than scale midpoint value, with the item during the ceremony, I felt that social status became irrelevant receiving the highest mean rating. In recently published studies where Kettner et al. explored collective psychedelic use during YD ceremonies in naturalistic settings (N = 886), they calculated the mean reported total communitas as the sum across the first 8 items in Fig. 7. They obtained values  Table SM6 contains indicative quotations for each theme; the SM includes the entire thematic analysis classification. Several of the participant statements (written in italics) are woven into the discussion that follows.

Discussion
Our results overwhelmingly suggest that the phenomenological intensity of STEs which arose for participants during Isness-D is comparable to YDs, in both naturalistic and laboratory settings. For example, Isness-D participants' average ICS score of 2.9 ± 1.4 (SD) is statistically indistinguishable from recent results published by Forstmann et al. 83   www.nature.com/scientificreports/ be used to cultivate spiritual sensitivities which avoid the "woo-woo" pseudo-scientific associations which can arise from YDs, e.g., which have recently been discussed by Carhartt-Harris and Friston 15 . Connectedness, which has been previously been highlighted as a key aspect of the YD experience 87 , emerged as the strongest qualitative theme for Isness-D participants, "I felt connected with myself but also with everyone else here… I think 'connected' is the word for me for the end of this session." Others commented on how Isness-D offered "A completely other way of connecting that I'm not familiar with, [where] all the usual stuff disappears." Comments like these are aligned with the observed change in ICS post-Isness versus pre-Isness. The matter-energy narrative of Isness-D and its aesthetics of luminosity-which blurred the boundaries of individual identity-seems to have been important for enabling participants to reimagine a sense of connectedness: "Connecting light and emotions as energy was special… light as connection; light transferring between matter; light creating memory. Experiencing myself and the other people in the group as light energy was joyful. It… allowed me to think about other spaces connecting in the world." While the experience of energetic coalescence was only one aspect of the Isness-D experience, it seemed to be particularly poignant and intimate for many people, with participants "struck with how quickly the abstract lights grew to hold tangible meaning… as other people. It was especially poignant [during moments of coalescence] when we moved toward the centre and felt as if we might collide or enter each other's personal space. " For many participants, coalescence facilitated a particularly strong sense of connectedness: "we could get closer than [in real-life] which felt more intimate, and connecting-nearly as much so as with a partner, child or pet-even though we were in different places. " Others observed how moments of coalescence produced somatic sensations: "I can just literally walk into people and it's quite sensuous", and also "I could feel subtle changes in my hands as if something was passing by, something physical. " Another commented how, during moments of coalescence "I got quite emotional… I got this surge of emotion where I don't know if I wanted to gasp or cry or what it was, but I was kind of shocked in awe. " Not a single participant reported being uncomfortable when others coalesced with their energetic essence; however, some worried they were invading others 'personal space': "You can sense an imaginary presence around the glowing light, and you give it space even though there is no barrier there, other than imagined. " Several commented on the sense of intimacy that accompanied coalescence "It felt really intimate. And I tried to connect to whichever light being it was and… I tried to join mudras, and I think I sat [as an energetic essence] inside a few of you. I hope that was OK" Many people reported a pleasant sense of spaciousness at the moment of coalescence, recognizing in that instant that conventional material boundaries did not apply, "I found it really strange at the start, when you were telling us to go closer and closer and then that weird boundary of where personal space is and all of a sudden it was like 'oh, actually this is kind of nice!' " Several participants commented on how the intimacy of the coalescence experience was balanced with a sense of innocence and purity, enabling "the sweetest tenderness or pure, childlike love…." Earlier in this article, we highlighted two phenomenological ingredients known to be important in STEs: (1) diminished self-salience arising from dissolving self-boundaries; and (2) a sense of unity with something beyond the self. Given the tight relational co-dependence of these ingredients (where one is required to characterize the other and vice-versa), we suggested that they are best realized in a multi-body context, given that the softness, fluidity, or porosity of a body's boundaries become most apparent by observing its relational interactions with another body. One participant succinctly articulated how they experienced this relational co-dependence during Isness-D: "you lose yourself in the connection to other people". Figure 6B and Fig SM1 show that the correlation between participants' sense of connectedness and their diminished sense of egoic identity was significantly stronger following Isness-D than it was beforehand: the diminished sense of ego entailed in "losing oneself " arises through the unique sort of "connection to other people" that Isness-D enables. The relational co-dependence between connectedness and diminished egoic identity is also seen in quotations like, 'Identity didn't matter anymore; it was about experiencing things together. That was wonderful.' As energetic essences, the implicit, explicit, conscious, and unconscious judgments that permeate social and relational interactions were diminished: 'I found [the anonymity] quite powerful because we were all completely equal in the space. Any of the pre-judgements that come from how people look, sound and that sort of thing just aren't there.' Stripping away these aspects of interpersonal interactions led to a sense of joy, purity, love, and beauty: 'I felt [when touching one another's heart centres] the sweetest tenderness or pure, childlike love… stripped back, without any of the assumed layerings that we place upon reality and relationships… just to the absolute core, it was truly beautiful. ' These comments support the exceptionally high scores observed for item 6 in the Communitas scale in Fig. 7 (During the ceremony, I felt that social status became irrelevant). Yaden et al. 5 note that the experience of self-loss is sometimes linked to pathologies (e.g., going back to the work of Freud); however, they argue that it is more often associated with positive outcomes. Our results are broadly aligned with this conclusion: the experience of self-loss for Isness-D participants was overwhelmingly positive.
Participants reported positive emotions and an overwhelming sense of calm and relaxation at the end of their Isness-D experience, "I feel remarkably happy. There's something very happiness-inducing about this whole practice." Some reported lower levels of anxiety and stress, observing that Isness-D helped them to "dissociate from something within you…I don't know if it… was my identity or my anxieties that I put on the side…" Another similarly commented, "I arrived feeling pretty anxious and disconnected from others, but after that experience I feel much more calm and hopeful. Hugely due to the focus of how energy doesn't truly disappear. I guess the fear of dying (the existential threat) is always on our minds, but that experience quieted the fear. " For some, Isness-D encouraged reflection on mortality, and their own energetic transience amidst a larger unfolding energetic process: "It's clear that the energy isn't just gone [in death], it goes somewhere. It's beautiful to think that it's all out there somewhere and it's still circling you constantly" and also "Seeing all the lights together reminded me of a dream I had years ago where the universe was ending and everybody was dissolving into balls of light and merging into one. So, I was like 'oh cool! The universe is ending. I'm cool with that.' " Some participants articulated how Isness-D offered a form of connectedness that contrasts COVID-related isolation: "[the sense of connection] is an experience I haven't felt in a while because of COVID and being in front of screens all of the time", and also "I feel like we've had a hug, and www.nature.com/scientificreports/ I haven't had many of those recently… a really nice thing to have". Another commented on how it helped them understand the difference between "stillness and stagnation…. This [pandemic] can make you feel very stagnant, cause you're in the same place and you're not still or rooted to anything." For many participants, positive emotions seemed to be associated with the sense of playfulness that arose from relaxed egoic identity: "It was…a space to be free, you could do whatever you want and not feel judged" and also "There certainly was a sense of playfulness in this, which was really nice. As adults that's not always something that we pursue." For the MEQ30, the Isness-D and Isness-C results for the I and T factors were statistically indistinguishable. For the M and P factors, the Isness-D scores were lower than Isness-C. The lower scores make sense for several reasons. First, the distributed 'citizen science' approach meant that we had significantly less control over participant's set and setting prior to entering VR and after leaving VR. Whereas in our previous study we were able to offer all participants the same context, the same preparatory environment, similar psychological priming, and a more consistent experience of the technology, this was not possible using the distributed citizen science approach, where the variability was more significant. For example, we had little ability to influence participant expectations or control how the node hosts described Isness-D to participants during the recruitment phase. Especially in the early stages, there was variability in host preparation: for example, there were some cases where participants from one node would be ready and waiting in VR for the beginning of Isness-D phase 1, whilst other node hosts were troubleshooting the technology (e.g., HMD fit, focus, fit of the OMG-VR gloves (see Methods), etc.). Second, whereas our previously published results were all obtained using a local area network where we could closely monitor latency and quickly solve any technical problems, the same was not true for the work described herein. During the early stages of our citizen science study, a number of participants had intermittent disruptions to their plausibility illusion 40,41 , as a result of unoptimized network instability issues. Third, whereas the previous participant cohort were drawn from attendees at a psychedelics & consciousness conference, the cohort for Isness-D was drawn from a broader distribution of international participants. Finally, Isness-D was effectively an adaptation of an experience that we originally designed to be co-located. Whilst we made several changes to accommodate the affordances of a multi-person distributed experience, our limited resources during the lockdown period made it difficult for us to undertake more extensive changes.
Isness-D differs from conventional YD psychotherapy in various ways. For example, the phase 1 preparation lasted ~ 15 min, far less than the preparation for studies carried out for YD psychotherapy, which typically include a total of 4-8 individual sessions (both before and after the YD session). The three phases of Isness-D last a total of ~ 70 min, shorter than psilocybin and LSD experiences, which can often last from 6-14 h. Finally, Isness-D was designed as a group experience, whereas most YD and VR studies are individual experiences. As discussed above, the distributed multi-body group aspect of Isness-D is clearly important for weakening ego boundaries, and fostering a sense of connectedness. The opening and closing interactions between the group and the facilitator (e.g., to develop the energy-matter narrative and encourage moments of energetic coalescence) were important, 'really help [ing] to hold the experience and make me feel more comfortable with everyone. ' Compared to previous YD studies, the N = 58 Isness-D sample size is reasonable; however, this study had a number of limitations. For example, while we were able to demonstrate significant changes post-Isness compared to pre-Isness, we did not carry out a control experiment, and therefore some of our analyses are primarily comparative. Whilst the 'citizen science' participant sample in this work clearly represents an improvement on our previous work, further work will be required to make definitive statements about the extent to which sample selection bias may have influenced our results. Our ability to compare the MEQ30 from this work with previous studies depends on the assumption that the baseline responses of our participant sample are not anomalously high or low, and within the range spanned by 6 previously published baseline studies. Figure 4 shows that these baseline studies have a broad MEQ30 score distribution. Our comparative statistical analyses ( Fig. 4 and Table SM2) show that the Isness-D results are more intense (p < 1E−6) than all of them. Nevertheless, we believe that the results described herein provide a degree of confidence in the results which we obtained in our previous study. As discussed above, the fact that the MEQ30 results for Isness-D are slightly lower than the results obtained for the co-located Isness-C experience is entirely aligned with our expectations, given the very different circumstances in which each study was conducted. At this stage, it is unclear how exactly to define a "placebo" for an experience like Isness-D, but this is an issue that would be interesting to investigate in future work. The design of Isness-D as a group experience suggests that the individual data may be correlated. In future studies we wish to investigate the correlation of the results obtained for participants within specific groups, and compare intra-group results to inter-group results. Given that the quantitative analysis was carried out retrospectively and not in relation to specific moments of Isness-D, we are somewhat limited in our ability to comment on how the narrative aspects of Isness may have primed participants for mystical-type, communitas and fusion experiences. Neither can we make conclusive statements regarding the extent to which the results in Figs. 4, 5, 6, 7 arise from specific components of Isness-D. In future studies, it would be interesting to address these limitations by carrying out control experiments in which aspects of Isness-D are systematically removed. This would enable us to understand in further detail the extent to which specific Isness-D components-the narrative, the immersive group aspect, the energetic coalescence, etc.-contribute to the overall results.

Conclusions
The aim of the work described herein was to determine whether the unique affordances of multi-person distributed VR can be used to reliably elicit intense STEs. Within this paper, we have described Isness-D, an experience we have developed to blur conventional self-other boundaries using the unique affordances of distributed multiperson VR. Built on a matter-energy narrative, Isness-D enables groups of participants distributed across the world to co-habit a shared virtual space and collectively experience their bodies as luminous energetic essences with soft spatial boundaries. It encourages participants to imagine themselves, others, and the world around them Scientific Reports | (2022) 12:8995 | https://doi.org/10.1038/s41598-022-12637-z www.nature.com/scientificreports/ as unfolding interconnected processes which are energetic (rather than fixed material entities). This fluid energetic representation enables participants to undergo moments of 'energetic coalescence' , a new class of embodied intersubjective experience whereby participants can have an embodied experience of including multiple others within their self-representation. To evaluate Isness-D, we adopted a 'citizen science' approach, coordinating a network of nodes distributed around the world to run multiple Isness-D sessions. This strategy enabled us to carry out this research amidst COVID related social distancing constraints. As a distributed approach, it was difficult to prime Isness-D participants in a controlled way. Nevertheless, analysis of Isness-D participant scores on four different self-report scales commonly used to assess YD experiences overwhelmingly suggest that the phenomenological intensity of STEs which arose for participants during Isness-D is comparable to previously published YD studies, in both naturalistic and laboratory settings. Isness-D enables participants to dissolve their sense of self in the connection to others, relaxing attachment to egoic identity and facilitating a strong sense of connectedness. For many participants, Isness-D offered a sense of intimacy, innocence, playfulness, and purity, eliciting a state of calm spaciousness. To the best of our knowledge, this work represents the first attempt to analyze a distributed multi-person VR experience using measurement scales which are typically applied to YD experiences. These results demonstrate that distributed VR can be used to elicit intersubjective STEs which simultaneously attenuate egoic identity and facilitate a sense of connectedness. This study reaffirms the ideas in our previous work 25 , where we speculated that it is possible to design phenomenology and experiences using numadelic technologies like multi-person VR to create the conditions for STEs from which participants derive insight and meaning. Distributed intersubjective VR experiences like Isness-D may have a role to play in easing unprecedented feelings of loneliness, isolation, and fear that have arisen with COVID restrictions. In future work, we hope to explore in futher detail the mechanisms responsible for the results obtained during Isness-D, and also carry out detailed follow-ups with participants in order to understand its impacts over the longer term.

Ethics. Ethics approval for the study described herein was obtained from the Faculty of Science Research
Ethics Committee at the University of Bristol (ethics approval code: 111003). Participants read an online study information sheet and completed a tick box consent form before signing up to an Isness-D session. The study was conducted according to the revised Declaration of Helsinki (2013) and the 1996 ICH Guidelines for Good Clinical Practice E6(R1). All participants were at least 18 years old, and consented to their data being gathered and published. To minimize participant risk, we adopted VR guidelines in line with those recommended by Madary and Metzinger 88 . All participants provided informed consent.
Software & hardware. Isness-D is an approximately 50-min experience with conceptual roots in earlier work by Glowacki and co-workers 25,[89][90][91] . As a fork of the Narupa project, Isness-D is designed around a client/ server architecture enabling each VR client access to the positional data of all the other participants, and a shared real-time molecular simulation of a 40-Alanine peptide macrocycle (40-ALA) whose dynamics are calculated in real-time using OpenMM, a GPU-accelerated computational biophysics engine 55 . Each participant can see through their headset a visual representation of both the molecular simulation and all of the other participants (e.g., as in Fig. 3). We designed the Isness-D experience to accommodate 4-5 participants wearing either the HTC Vive Pro or Valve Index HMD. Our cloud computing resources enabled us to mount our server on GPU shapes available at three different data centres: Frankfurt (Germany), London (UK), and Ashburn (Maryland, USA). Prior to entering Isness-D, participants at each node were fitted with the Open Mudra Gloves for Virtual Reality (OMG-VRs), etextile gloves that sense when a user pinches together their thumb and index finger, or thumb and middle finger to form a "mudra" position 13 . The OMG-VRs were designed to enable research scientists and students to 'grasp' and 'manipulate' molecular objects whose essence is purely energetic. They provide good positional tracking of the point at which a pinch takes place, require no calibration, and proved sufficiently robust to withstand use across the international network of Isness-D nodes in Fig. 3C. The OMG-VRs played an important role during the Isness experience. By adopting a 'mudra pose' (bringing the tip of their thumb in contact with the tip of either their forefinger or middle finger), Isness-D participants could generate light and collectively sculpt the dynamics of the tangible molecular object. The in-world rendering of participants' bodies is designed so that the intensity of the light generated during moments of energetic coalescence depends on the overlap between energetic bodies. For example, two coalesced bodies generate more light than a single body, and four coalesced bodies generate more light than two.
Isness nodes and citizen science design. Each of the 14 different nodes distributed across the world ( Fig. 3C)  www.nature.com/scientificreports/ modate participants, this list of timeslots was placed into a web scheduler, with a link that was then circulated to participants. After receiving the web link, participants were able to identify the various session times available at their host node. Before registering for an Isness-D time slot, they first had to read the online study information sheet and complete a tick box consent form. We based our data collection on the structure of the psychedelic ceremony study 92 , which involves a 'pre-ceremony' and a 'post-ceremony' component and includes a combination of psychometrics commonly used in psychedelic research studies. The online pre-Isness-D questionnaire (using the survey platform Qualtrics) consisted of the ICS scale 56 and items relating to participants' openness to the upcoming experience. The post-Isness-D questionnaire included: (1) the ICS scale 56 ; 2) the MEQ30 26,27 3) the EDI 84 ; and 4) the Communitas questionnaire 61 . Additionally, participants were asked questions related to bodily effects and demographics. We added to the post questionnaire a final comments section where participants were able to leave written feedback. Since it was designed to evaluate YD experiences, not all aspects of these pre-and post-ceremony questionnaires were relevant to Isness-D. To ensure its relevance, we undertook a number of modifications. For example, we removed any questions asking about suffering and surrendering; we judged these to be less relevant to the Isness-D experience and moreover our preliminary analysis suggested that they increased anxiety among participants. The final versions of the pre-and post-Isness-D questionnaires used to evaluate Isness-D are given in the SM. Participants received an email link to a digital version of the pre-Isness-D questionaire approximately one hour prior to entering into Isness-D, and another link to the post-Isness-D questionaire after the conclusion of their session.

Isness-D experience design.
Participants experienced Isness-D as a 3-phase journey, composed of a preparatory/induction phase, the Isness-D session, and an integration/reflection phase, adopting a similar model to that which we have utilized previously, and which is based on the model utilized in YD assisted psychotherapy 22 . An Isness-D session involved a facilitator along with 3-5 participants, each of whom simultaneously connected into the Isness server from one of the nodes shown in Fig. 3. In some cases, additional observers were present within the Isness-D environment: by utilizing 'spectate' mode, they were invisible to the participants and to the facilitator. As technical assistants, observers played an important role in helping to stabilize the Isness-D experience. The 'spectate' function also helped us to train new facilitators, giving them the opportunity to observe first-hand without affecting the participant experience. All of the Isness-D nodes had available a play space of approximately 2 m × 3 m. To align participants within the shared virtual space, we adopted the following radial orientation strategy: we placed at the center of each node's space a vector that pointed toward one of the long (3 m) boundaries, aligned each node's space so that their centers were coincident and the vectors were aligned, and then rotated each node's vector to make an angle of 360/n° with respect to its neighbors (where n is the number of participants). Figure 3 shows the resulting orientation for our most typical arrangement, with n = 4 participants. Isness-D was constructed from a set of 'aesthetic hyperparameters' , each of which controls some aspect of the participants' phenomenology and can be precisely varied using an available set of on-screen sliders. We defined a phenomenological 'state' as a given set of aesthetic hyperparameter values with some specified time duration. Each phase of Isness-D is comprised of a set of states, whose hyperparameter values are saved to a JSON file to ensure reproducibility. The Isness-D experience involved varying 69 different aesthetic hyperparameters, including for example: the color, distribution, density, and latency of the energetic bodies; the size of the heart center light; the rendering options for the energetic thread shown in Fig. 3; options for setting interactive forces to achieve different effects; the state duration, and the global light levels. Our decisions on how to set the aesthetic hyperparameters were grounded in the various design considerations outlined in our previous work 25 . Each phase of Isness-D involved two audio components (played through the HMD earphones): (1) communication with the other participants in the space, which was achieved using an audio-only Zoom call using the mic built-in to the HMD; and (2) a pre-recorded narrative soundtrack, which we streamed as a separate audio channel using Twitch (www. twitch. tv). In what follows we describe the various phases of the Isness-D experience in further detail.
Pre-experience technical checks. At the beginning of a scheduled Isness-D session, the node hosts would: (1) connect to the cloud mounted Isness server using an I.P. address provided by the session facilitator; (2) connect to an audio-only zoom call, using the earphones and mic in their HMD, and (3) connect to the Twitch feed. After the hosts confirmed that all of the relevant equipment was either plugged in or charged up, they would then enter into the Isness-D virtual environment with the facilitator prior to any participants entering and carry out: (1) an interaction latency check, to identify nodes whose latency or fluidity was significantly different from others as a result of a slow internet connection or network instability; (2) an OMG-VR check, verifying that the gloves generated light through the mudra pose, and were able to interact with the dynamical molecular object; (3) an audio check, where the facilitator ascertained whether any of the host nodes were suffering significant Twitch latency; (4) a spatial orientation check, where each node host confirmed that their respective radial orientation within the Isness-D virtual space minimized cable trip hazards. Hosts were asked to ensure that their participants were not in the same room while these checks were performed; however, this was not possible in many cases owing to space constraints.
We found Isness-D offered a fluid and stable experience so long as the internet connection at each node was stable. Persistent packet loss was the most substantial technological challenge to a smooth Isness-D experience. Technical instabilities were more common during the first week of our studies. Early on, we realized that the fluidity and reliability of an Isness-D experienced depended less on network speed and more on network stability (i.e., reliability low jitter, and low packet loss). We encouraged our nodes to evaluate their network stability using a simple online test 93 , and undertake optimization where possible-e.g., using a wifi router rather than tethering to a phone, moving closer to the wifi router, using an ethernet cable where possible, and encouraging www.nature.com/scientificreports/ other network users to avoid heavy usage during an Isness-D session. This combination of measures significantly reduced network-related glitches, creating a robust set of distributed nodes, and led to an increase in the number of sessions that we judged to be technologically seamless.
Phase 1: preparation. Once the facilitator was satisfied that each node passed the technical checks outlined above, they invited each host to fit their participants with the HMDs and a pair of OMG-VRs, and ensure they were in a comfortable seated or kneeling position. When all the participants were ready, the facilitator initiated the Isness-D state sequence and Twitch audio stream. Over the next ~ 15 min (7 aesthetic states), the facilitator: (1) introduced themselves and greeted the participants; (2) asked each of the participants to introduce themselves, saying where they were located physically and how they were feeling; (3) instructed participants to alert their hosts if at any stage they suffered network problems or wished to leave; (4) thanked the participants for participating in our data collection efforts, and (5) asked that everybody respect the anonymity of the others. Next, the facilitator invited each of the participants to stand up and participate in some gentle movement exercises, to help them develop familiarity with moving in Isness-D and interacting with others. Participants were led in the following sequence: (1) acknowledging the others in the space by placing their hands in a prayer position and bowing to one another; (2) three rounds of gently raising and lowering their arms, with synchronized inhalation and exhalation; (3) rapport-building where each participant in turn was offered the opportunity to make up a motion which the others could then simultaneously mimic; and (4) encouraging each participant to gradually move toward the center of the virtual space ("a little closer… a little closer… a little closer…") until they all overlapped in order to have the experience of coalescing their energetic body with the other energetic bodies (Fig. 3A,B). The facilitator then invited the participants to return to a comfortable seated or kneeling position, practice generating light using their OMG-VRs, and sculpt the dynamics of the molecular thread. Expanding the scale of the molecular thread, the facilitator encouraged all the participants to hold onto it, creating a circular shape (Fig. 3A.i). The facilitator invited the participants to imagine Isness-D as a kind of meditation on the relationship between matter and energy, and how new senses of interconnectedness might emerge from imagining ourselves, others, and the world around us as luminous energetic essences rather than rigid material objects. The molecular thread was described to participants as a kind of tangible dynamical organism which-because its essence was purely energetic-could connect our energetic essences across different physical locations and time zones. Whilst holding the molecule, the facilitator framed an intention-setting exercise, inviting each participant in turn to say a few words about something to which they would like to connect, inviting them to imagine the molecule as an energetic vessel to contain these intentions. Phase 2: A loosely guided choreography. As the visual display of each participant faded to darkness, the facilitator went into 'spectate' mode, and another narrator took over. For the next 25 min, participants were guided through a pre-recorded narrative soundtrack played through the HMD headphones. The narrative began with a short visualization, inviting participants to close their eyes and imagine their breath as radiant light concentrated at their heart center. After being instructed to open their eyes, the participants were guided through 16 states, each composed from a different combination of aesthetic hyperparameters. The narrative journey was an adaptation of the experience we previously developed for Isness-C 25 , but reworked in order to accommodate the unique affordances of the distributed environment. The experience took participants through a loose narrative of energetic emergence, dissipation, and fluctuation. It was designed to balance moments of individual introvertive exploration with collective extrovertive interaction. The narrative was accompanied by a soundtrack designed to broadly reflect the arc of the journey. Whilst in 'spectate' mode, the facilitator kept watch over the virtual space, in order to discern signs of potential technical difficulties, contact the relevant hosts in cases where there may be technical problems, and gently remind participants who became particularly excited that they should move gently, carefully and fluidly.

Phase 3: integration & discussion.
Once the pre-recorded narrative finished and the participants were once again in a comfortable seated or kneeling position, the facilitator then re-emerged from spectate mode, making themselves visible to the Isness participants and gently greeting them. As during phase 1, the facilitator altered the scale of the energetic molecular organism and encouraged all the participants to hold onto it, forming an energetic circle which connected together the participants, as shown in Fig. 3A.i. Over the next 10 min, the facilitator encouraged the participants to share any thoughts, feedback, emotions, or impressions which arose during the experience. These group discussions were undertaken to enable maximum conversation amongst the participants, with the facilitator's role as that of an active listener. The format of these group discussions was not rigidly prescribed; instead, the emphasis was on enabling conversation amongst the group. In many groups, participants did not immediately wish to speak. In such cases, the facilitator asked a simple question to initiate the conversation-e.g., 'How do you feel?'; 'What remains?'; 'Were you aware of the others in the space with you?' Each of these discussions was recorded on Zoom, and subsequently transcribed as part of our data gathering efforts. After the discussion finished, the facilitator instructed participants in how to gently exit VR. Specifically, the participants were instructed to: (1) close their eyes; (2) gently remove their HMDs and place them on the ground next to them; (3) lie down; (4) let the light and sound in the ambient environment seep back into their conscious awareness; (5) open their eyes when they were ready; and (6) undertake the reflective writing on the paper and pencil provided from their host. This anonymized free writing was then photographed by hosts and sent to us for qualitative analysis. Those who were not ready to write in their post-Isness emotional state had the option to do so in their own time.