Cordyceps inhibits ceramide biosynthesis and improves insulin resistance and hepatic steatosis

Ectopic ceramide accumulation in insulin-responsive tissues contributes to the development of obesity and impairs insulin sensitivity. Moreover, pharmacological inhibition of serine palmitoyl transferase (SPT), the first enzyme essential for ceramide biosynthesis using myriocin in rodents reduces body weight and improves insulin sensitivity and associated metabolic indices. Myriocin was originally extracted from fruiting bodies of the fungus Isaria sinclairii and has been found abundant in a number of closely related fungal species such as the Cordyceps. Myriocin is not approved for human use but extracts from Cordyceps are routinely consumed as part of traditional Chinese medication for the treatment of numerous diseases including diabetes. Herein, we screened commercially available extracts of Cordyceps currently being consumed by humans, to identify Cordyceps containing myriocin and test the efficacy of Cordyceps extract containing myriocin in obese mice to improve energy and glucose homeostasis. We demonstrate that commercially available Cordyceps contain variable amounts of myriocin and treatment of mice with a human equivalent dose of Cordyceps extract containing myriocin, reduces ceramide accrual, increases energy expenditure, prevents diet-induced obesity, improves glucose homeostasis and resolves hepatic steatosis. Mechanistically, these beneficial effects were due to increased adipose tissue browning/beiging, improved brown adipose tissue function and hepatic insulin sensitivity as well as alterations in the abundance of gut microbes such as Clostridium and Bilophila. Collectively, our data provide proof-of-principle that myriocin containing Cordyceps extract inhibit ceramide biosynthesis and attenuate metabolic impairments associated with obesity. Moreover, these studies identify commercially available Cordyceps as a readily available supplement to treat obesity and associated metabolic diseases.

www.nature.com/scientificreports/ and cardiovascular diseases [17][18][19][20][21][22][23][24][25][26] . These findings suggest that targeting ceramide biosynthesis may offer a novel means to treat obesity and associated comorbidities. Pharmacological agent myriocin, a selective and potent inhibitor of serine palmitoyl transferase (SPT), the first enzyme in the ceramide biosynthesis pathway has been a workhorse reagent demonstrating a potent efficacy in inhibiting ceramide synthesis and preventing as well as reversing obesity and associated diseases in rodents 7,9,15,27 . Myriocin was originally isolated from fruiting bodies of the fungus Isaria sinclairii (辛克莱棒束 孢) and since then has also been found in a number of closely related fungal species such as the Cordyceps 28,29 . Despite having potent efficacy in rodents, purified myriocin is not an approved drug for human consumption. However, extracts from Isaria sinclairii and Cordyceps sinesis have been an essential component of traditional Chinese medication that has been consumed for thousands of years for the treatment of numerous indications, including diabetes [30][31][32][33][34][35] . Cordyceps gained worldwide prominence after 1993 following admittance from world record breaking Chinese long-distance runners that they consumed Cordyceps tonic during their training periods 30,32,33,36 . Since then, Cordyceps extracts have been increasingly sold as nutritional supplements in numerous countries, including United States and are being consumed by a large population globally. Numerous studies in rodents have found Cordyceps to reduce fat content, improve insulin sensitivity, hypertension and resolve hypertriglyceridemia and hypercholesterolemia [37][38][39][40][41] . In small clinical studies, the Cordyceps extract was found to ameliorate nephrotoxicity 42 . Moreover a follow-up study found that long-term-consumption of Cordyceps extracts for up to 3-years improved kidney function 43 . Importantly, genotoxic studies revealed that these extracts had no mutagenicity and is listed as one of the safest drug class (Class 1 drug) in the American Herbal Products Association Botanical Safety Handbook [44][45][46] . Despite, being safe and obvious therapeutic benefits of Cordyceps extracts, relatively nothing is known about the mechanisms via which Cordyceps exhibits these beneficial effects on energy and glucose metabolism. Hence, Cordyceps extracts are not approved for human consumption for the treatment of metabolic diseases.
Since the ceramide biosynthesis inhibitor myriocin is extracted from Cordyceps, we hypothesized that, myriocin might be the bioactive ingredient in Cordyceps and that ensuing reductions in ceramide biosynthesis might account for the beneficial effects of Cordyceps on energy and glucose metabolism. Screening commercially available Cordyceps extracts from Asia and the United States via mass spectrometry, we demonstrate that myriocin is indeed present in Cordyceps extracts but at variable concentrations. Moreover, treatment of mice with an obesogenic diet supplemented with myriocin containing Cordyceps at a dose equivalent to that currently being consumed by humans, reduces ceramide accumulation and improves energy homeostasis, insulin sensitivity and resolves hepatic steatosis with an alteration in the gut microbial ecology. Collectively, these studies provide proof-of-principle data in rodents supporting investigations on the therapeutic utility of commercially available myriocin containing Cordyceps to inhibit ceramide synthesis for the treatment of obesity, insulin resistance and hepatic steatosis.

Results
Identification of commercially available myriocin containing Cordyceps extracts. There are over 750-species of Cordyceps. Among these, Cordyceps sinesis is the species that is most famous and classified as drugs in the Chinese Pharmacopeia 47 . Extracts isolated from these species of Cordyceps have been previously reported to vary in medicinal efficacy due to regions where these are cultivated and are increasingly in short supply due to difficulties in collecting natural Cordyceps that are grown at 3000-4000 meters above sea levels around the Himalayan mountains in Tibet and high regions of China 30,36 . The development of recent technologies that enable artificial cultivation and production of Cordyceps has enabled to overcome the supply limitations and to some degree the heterogeneity in Cordyceps composition, such that extracts from these species of Cordyceps are now available as supplemental medicinal capsules globally including via various E-commerce platforms 30,36 . Moreover, these artificially cultivated Cordyceps were found to possess similar potency to those grown in wild 48,49 . Here we investigated firstly, whether myriocin, a pharmacological inhibitor of serine palmitoyl transferase (SPT), the first enzyme in the ceramide synthesis pathway, that is present and, in some cases, isolated from Cordyceps 28,29 , is enriched in commercially available Cordyceps extracts approved for human consumption. Secondly, if myriocin is present in commercially available Cordyceps extracts, do different extracts contain equivalent amounts of myriocin. Therefore, we acquired eight different artificially grown Cordyceps extracts or wild type Cordyceps packaged as capsules or sold as wild mushrooms (Sample 1-8, Table 1) commercially available in United States and Asia (Singapore and China) and subjected them to mass-spectrometry analysis ( Supplementary Fig. 1A). This analysis revealed that myriocin is indeed present in commercially available Cordyceps extracts and its content is highly variable in different Cordyceps preparations ( Fig. 1A-D, Table 1 and Supplementary Fig. 1A-F). For example, sample 6 did not contain any traces of myriocin, sample 2-5 and 7 contained 0.5-2 nmol/g myriocin and sample 1 and 8 contained 4-6 nmol/g myriocin ( Fig. 1A-D, Table 1 and Supplementary Fig. 1A-F).
Treatment of mice with myriocin containing Cordyceps extract reduces serum ceramide content in obese mice and does not alter intestinal morphology. Treatment of mice with myriocin, inhibits ceramide biosynthesis and increases thermogenesis and energy expenditure, improves glucose homeostasis and resolves hepatic steatosis 27,50 . Therefore, we tested whether treatment of mice with Cordyceps extract containing myriocin at a dose that is recommended for human consumption resolves metabolic impairments associated with obesity. To this end, we sought to test the sample 1 since it is a capsulated Cordyceps extract that is artificially cultivated in United States, requires no further processing and is readily available for human consumption, whereas sample 8 that contained comparable amount of myriocin is a wild type Cordyceps acquired from China and needs further processing to prepare extracts. www.nature.com/scientificreports/ To test the efficacy of identified Cordyceps sample 1, in improving glucose homeostasis and nutrient metabolism, powdered sample 1 at a dose of 19.7 mg/kg was mixed into the high fat diet (HFD) as previously described 40 . This dose was calculated on the basis of pharmacological dose of sample 1 that was recommended by the manufacturer for human consumption. We also used a cohort with a higher dose (39.5 mg/kg) and observed intestinal inflammation, edema indicating intestinal toxicity at this dose and hence the study was discontinued. As controls we used high fat diet that either contained an equivalent amount of starch derived from rice or Cordyceps sample 6 that did not contain any traces of myriocin. Feeding 4-weeks old C57Bl6/J mice with HFD supplemented with sample 1 reduced circulating ceramides, dihydroceramides, dihydrosphingomyelin, sphingomyelin and glucosylceramides content as early as 4-weeks an effect that was sustained for the remaining 12-weeks of the treatment ( Fig. 2A-E). In contrast sample 6 did not display any changes in serum ceramide levels compared to controls ( Fig. 2A-E). Moreover, treatment with samples 1 but not sample 6 elevated diacylglycerol accumulation after 4-weeks but were not altered after 12-weeks (Fig. 2F). Taken together, these data suggest that sub-chronic  www.nature.com/scientificreports/ and chronic treatment with HFD-supplemented with myriocin containing Cordyceps extract selectively inhibit synthesis of sphingolipids and its precursor ceramide. Pharmacological and genetic inhibition of SPT presents with intestinal toxicity and alters intestinal morphology 51,52 . Therefore, we investigated whether treatment of mice with sample 1 Cordyceps extract had any impact on intestinal morphology. Histological analysis of the intestine following treatment with Cordyceps extracts from both sample 1 and sample 6 did not exhibit any alterations in gross intestinal morphology (Fig. 2G). Independent pathological assessment, further revealed no intestinal toxicity in animals treated with either sample 1 or sample 6 ( Fig. 2G).
Treatment of mice with myriocin containing Cordyceps increases energy expenditure in obese mice. Treatment of C57Bl6/J mice with HFD-supplemented with sample 1 reduced ~ 30% body weight ( Fig. 3A,B) and altered lean/fat mass ratios (Fig. 3C), the phenotype that is consistent with our previous study following whole-body inhibition of SPT with the pharmacological agent myriocin 27 . In contrast, treatment with HFD-diet supplemented with sample 6, that lacks myriocin increased ~ 20% body weight and lean fat mass ratio ( Fig. 3A-C). Consistent with reductions in fat mass observed, sample 1 treatment reduced weights of vari- www.nature.com/scientificreports/ ous adipose tissue depots such as the epididymal adipose tissue (eWAT) and the subcutaneous adipose tissue (sWAT) (Fig. 3D). In contrast treatment with sample 6 resulted in significantly heavier liver despite exhibiting reduced epididymal white adipose tissues (eWAT) weight (Fig. 3D). Treatment with sample 1 exhibited increases in oxygen consumption (VO 2 ), carbon dioxide (VCO 2 ) production, and energy expenditure without altering food intake ( Fig. 3E-G) as early as following 4-weeks of Cordyceps treatment when the animals were equally obese. Interestingly, the increases in energy expenditure were sustained in sample 1 treated groups even after 12-weeks of HFD-treatment ( Fig. 3H-J). No effect was observed on food intake, respiratory exchange ratio (RER), or locomotor activity following these interventions (Supplementary Fig. 2A-H). Surprisingly, Sample 6 that lacks myriocin did not alter any of these parameters after either 4-weeks or 12-weeks of treatment ( Myriocin containing Cordyceps alters adipose tissue morphology and increases adipose tissue thermogenic program. Histological assessment of different adipose tissue depots suggested that treatment with sample 1 produced a broad spectrum of effects on adipocyte morphology. Adipocyte cross-sectional area was significantly smaller (~ 50%) in sWAT and BAT depots indicating they are more metabolically active (Fig. 4A). By comparison, treatment with sample 6 exhibited increase in BAT adipocyte sectional area and had no changes in adipocyte size in the sWAT depot (Fig. 4B).
Our previous studies have identified that ceramides impair adipose tissue thermogenic program and conversely treatment of mice with myriocin increased adipose tissue thermogenic program and enhanced wholebody energy expenditure 27,50,53 . Therefore, we further determined if the improvement in energy expenditure following treatment with sample 1 increases adipose tissue thermogenesis. To this end, we performed immunohistochemistry for the thermogenic protein, uncoupled protein-1 (UCP1) in the sWAT and BAT. Consistent with www.nature.com/scientificreports/ the prior myriocin studies 27 , treatment with sample 1 induced UCP-1 expression in sWAT and BAT (Fig. 4C). Conversely, sample 6 treatment did not alter UCP-1 content in adipose tissues (Fig. 4C).
Treatment of mice with myriocin containing Cordyceps improves insulin sensitivity and resolves hepatic steatosis in obese mice. We further assessed the efficacy of sample 1 in resolving metabolic impairments associated with obesity. Treatment of obese mice with sample 1, exhibited moderate improvements in random fed blood glucose as early as 3-weeks after the treatment, an effect that was sustained and significantly improved following 12-weeks of treatment (Fig. 5A). Importantly, sample 1 reduced fasted blood glucose following 12-weeks of treatment (Fig. 5B). Moreover, sample 1 treatment improved glucose tolerance (Fig. 5C) and insulin response during the insulin-tolerance test (Fig. 5D) without altering serum insulin content ( Supplementary Fig. 2I). In contrast, sample 6 treatment did not affect fed and fasted blood glucose, exhibited no differences in glucose tolerance but impaired insulin tolerance, and exhibited elevated serum insulin levels (Fig. 5B,C and Supplementary Fig. 2I).
Ceramides impair systemic insulin sensitivity by inhibiting glucose uptake in peripheral tissues and compromising hepatic glucose suppression 2,54 . Moreover, treatment with myriocin, negates these metabolic effects 7,50,53 . We and other have previously found that these effects are mediated through the ceramides ability to inhibit www.nature.com/scientificreports/ insulin-stimulated Akt/PKB phosphorylation, an effect that is restored by inhibition of ceramide biosynthesis with myriocin 7,50,53-57 . Therefore, we further determined if the observed metabolic improvements in glucose metabolism caused by sample 1 were associated with improved signaling to Akt/PKB. Herein we found that consistent with prior myriocin studies 7,9,15 , treatment with sample 1 significantly enhanced hepatic Akt/PKB phosphorylation (Fig. 5E). In contrast, treatment with sample 6 did not have an effect on Akt/PKB phosphorylation (Fig. 5E). Assessment of the histological sections from the liver revealed, marked reduction in lipid droplets in mice treated with sample 1, confirming the resolution of hepatic steatosis (Fig. 5F). Consistent with reductions in hepatic lipid droplet content, this intervention also reduced hepatic triglyceride content (Fig. 5G). By comparison, treatment with sample 6 did not alter hepatic triglyceride content but increased lipid droplet size, implying worsening in hepatic steatosis (Fig. 5F,G).

Treatment of mice with myriocin containing Cordyceps alters the composition of gut microbiota.
Recent studies suggest that gut microbiome could affect metabolic health and may affect insulin resistance and lipid metabolism 58 . Notably, accumulated evidence also suggests an association between dysregulated intestinal microbiome with obesity, insulin resistance and, therefore, type 2 diabetes 59 . To determine whether ceramides illicit a lipotoxic effect by modulating intestinal microbiota, we performed 16S rRNA gene sequencing to identify the composition of the gut microbiota in cecum samples isolated from animals treated with either control, Sample 1 and Sample 6. These studies revealed that although α-diversity, an indicative of species richness and evenness, was similar among the groups (Fig. 6A), the β-diversity, which represents the phylogenetic compositional differences, was significantly different at the OTU level in between the groups (Fig. 6B). Further, microbial profiling revealed significant alterations in the composition of microbes at different taxonomic levels following Sample 1 and Sample 6 treatment (Fig. 6C). Notably, Sample 1 (myriocin containing Cordyceps) treatment induced beneficial changes in gut microbiota as determined by a reduced abundance of Clostriduim genus (belong to the phyla Fermicutes) (Fig. 6D) which is negatively correlated with obesity 60 . Moreover, this intervention resulted in increases in the abundance of the genus Bilophila (belong to phyla Proteobacteia) (Fig. 6D) which is associated with reductions in blood glucose and improved insulin sensitivity 61,62 . In contrast, treatment with Sample 6 did not exhibit any changes in these beneficial microbes and altered the abundance in numerous microbes associated with worsening of metabolic syndrome [Bacteriodetes (Bactereroides, Odoribacter), Firmicutes (Anaerostipes, Dehalobacterium and Dorea)] despite increment in beneficial microbes such as the Verrucomicrobia (Akkermansia)) ( Supplementary Fig. 2J).
We further performed Spearman's correlation analysis to determine the association between the selected genera with metabolic parameters such as body weight, body fat, fasting blood glucose and fed blood glucose. Twelve specified genera were significantly associated with one or more of the metabolic parameters (Fig. 6E). In addition, eight unspecified genera belonging to the family Coriobacteriaceae, Desulfovibrionaceae, F16, Mycoplasmataceae, Mogibacteriaceae, Christensenellacea were associated with the metabolic parameters (data not shown). Notably, Clostridium was found to be positively associated with body weight, body fat, fasting blood glucose or fed blood glucose (Fig. 6E). Conversely, Bilophila was found to be negatively correlated with body weight and fasting or fed blood glucose (Fig. 6E).

Discussion
Cordyceps extracts consumed as part of traditional Chinese medication regimens have previously been shown to ameliorate various diseases including metabolic anomalies 30 . However, it is not clear what is the bioactive ingredient present in Cordyceps extracts and what are molecular mechanisms through which these extracts elicit beneficial effects. Here we demonstrate, that presence of myriocin, a pharmacological inhibitor of ceramide biosynthesis in Cordyceps extract is critical for its beneficial metabolic effects. We further demonstrate that treatment of mice with Cordyceps extract containing myriocin at a currently recommended dose of Cordyceps for human consumption, reduces ceramide biosynthesis, alters gut microbial ecology and has profound effects on increasing energy expenditure, insulin sensitivity and resolving hepatic steatosis.
Ceramides accrue in obesity and recent studies from us and others suggest that these are toxic lipid metabolites that causes insulin resistance 2,7,27,50,53,[63][64][65] . Moreover, our recent studies have suggested that ceramides are key nutrient signals that regulate energy storage and utilization 5,50 . Furthermore, inhibition of ceramide biosynthesis using myriocin in rodents improves various features of metabolic diseases such as insulin sensitivity, hepatic steatosis and cardiovascular complications 4,9,15,66 . Given the beneficial effects elicited by inhibition of ceramide biosynthesis, targeting ceramide biosynthetic pathway offers a novel strategy for the treatment of obesity and associated comorbidities. Despite this wealth of knowledge in rodents and other mammals, literature is devoid of any ceramide intervention studies that includes myriocin usage in humans. However, Cordyceps extracts, one of the fungi species where myriocin was originally extracted from has been used in traditional Asian medicines for centuries for multiple indications including diabetes 30 . The present study identified some of the naturally occurring or artificially grown and readily available Cordyceps extracts contain myriocin and suggest that a substantial number of individuals might already be consuming these myriocin enriched extracts and perhaps benefiting from the inhibition of ceramide biosynthesis. Future human intervention studies with commercially available Cordyceps extract such as the sample 1 are highly warranted to determine whether indeed myriocin containing Cordyceps extract inhibits ceramide biosynthesis and alters glucose and energy metabolism.
Systemic inhibition of ceramide biosynthesis increases energy expenditure by elevating adipose tissue thermogenic program and improves glucose uptake and metabolism by regulating Akt/PKB activation 2,3,5 . We identify Cordyceps extract from sample 1 indeed elicits its beneficial effects by elevating thermogenic program in adipose tissue and glucose uptake and metabolism via regulation of Akt/PKB activation (Figs. 4C, 5E) www.nature.com/scientificreports/ www.nature.com/scientificreports/ of ceramide biosynthesis using Cordyceps extract from sample 1 inhibits numerous sphingolipids including, sphingomyelin, that would argue whether observed improvements are due to ceramides or another sphingolipid intermediate. Given that, ceramides but no other sphingolipids modulate thermogenic program in adipocytes and Akt/PKB activation 27,50,53 , we think these improvements were driven by exclusive inhibition of ceramides but no other sphingolipids. Surprisingly, we find that sample 6 that does not contain myriocin induces ceramide accumulation (Fig. 2B) and ensuing negative metabolic effects associated with ceramide accumulation such as decreases in energy expenditure and increases body and liver weight (Fig. 3A-J). Given that the active chemical composition in most of the commercially available Cordyceps preparations are not very well characterized 67 , future studies are warranted to identify active chemical compound in sample 6 that drives ceramide biosynthesis. Moreover, these data indicate that additional screening procedures should be implemented to ensure that Cordyceps extracts that are commercially available are safe without adverse effects. In addition to the known mechanisms of ceramides action, the present study identifies alterations in the composition of gut microbiota following ceramide inhibition and identifies two genera Clostridum and Bilophila as the key microbes that are altered following ceramide inhibition and were found to be correlated with either body weight, fat mass and fasting blood glucose or fed blood glucose (Fig. 6D, E). Though we identified beneficial microbial changes that potentially influences systemic metabolism, we cannot rule out the possibility that these changes are secondary to a healthier gut resulting from ceramide inhibition. Therefore, future studies will seek to elucidate if these microbes contribute to some of the beneficial metabolic homeostasis attributed to ceramide inhibition.
Notably, therapeutically targeting SPT, the first enzyme in the ceramide synthetic pathway hit a road block as genetically ablating SPT presents with lethality 68 . These effects seem to be accounted by the requirement of ceramides in maintaining intestinal homeostasis 51,68 . Similarly, pharmacological inhibition of SPT, with a new class of compounds results in intestinal toxicity 52 . Despite these limitations, numerous studies have repeatedly shown robust efficacy of myriocin in inhibiting SPT activity, reducing ceramide biosynthesis and resolving various metabolic indices [6][7][8][9][10][11][12][13][14][15][16] . We think, the toxicity associated with SPT inhibition results from complete inhibition of SPT, as mice lacking only one allele of SPT are viable without any major developmental effects and exhibit reductions in ceramide accrual and improvements in insulin sensitivity 69 . Similarly, high dosage of Cordyceps containing myriocin induces intestinal toxicity compared to lower dose ( Fig. 2G and data not shown). Therefore, the low dosage of Cordyceps containing myriocin similar to the one tested in this study, that has been consumed for centuries might provide a therapeutic opportunity to safely inhibit SPT, to reduce ceramide accumulation and improve insulin sensitivity. Moreover, purified low-dose myriocin that inhibits ceramide biosynthesis without eliciting intestinal toxicity might present an alternative strategy to inhibit ceramide biosynthesis and improve metabolic indices in humans.
Collectively, we identify that various commercially and readily available Cordyceps extracts currently being consumed by humans contain ceramide inhibitor myriocin and provide proof-of-principle studies that inhibition of ceramide biosynthesis with Cordyceps extract containing myriocin might be a readily available therapeutic option for the treatment of metabolic diseases. Moreover, given that Cordyceps have been thoroughly examined for not inducing mutagenicity and been assigned one of the safest supplements, these supplements might offer first-in-class ceramide intervention drugs for the treatment of metabolic diseases. However, future well-controlled studies in humans are warranted to determine if indeed Cordyceps treatment is safe and effectively inhibit ceramide biosynthesis and improve metabolic outcomes.

Materials and methods
All methods were performed in accordance with relevant guidelines and regulations.
Animal care. All animal procedures were conducted in compliance with protocols approved by the Institutional Animal Care and Use Committee (IACUC) at University of Utah and performed in accordance with the National Institute of Health Guide for Care and Use of Laboratory Animals. The experiments were complied with the ARRIVE guidelines 70 . C57Bl6/J male mice were purchased from Jackson Laboratories and were housed in groups of 3-5 at 22 °C-24 °C using a 12-h light/12-h dark cycle. Animals had ad libitum access to water at all times. Animals were fed a high fat diet (HFD) (D12492; Research Diets Inc., New Brunswick, NJ) supplemented with either starch or Cordyceps extract as stated in the manuscript from the age of 4-weeks as indicated. Euthanasia was performed using the CO 2 inhalation a method that is consistent with the euthanasia guidelines of American Veterinarian Medical Association and approved by Institutional Animal Care and Use Committee (IACUC).

Glucose-and insulin-tolerance tests.
Glucose-tolerance tests were performed in 8-week-old mice fed with Cordyceps supplemented diets for 4-weeks after an overnight fast. Glucose was injected (intraperitoneal injection of a 20% solution, 10 mL/kg body weight) and blood glucose concentrations in blood were measured after 0, 15, 30, 60 and 120 min with a glucometer (Bayer Contour, Bayer, Germany). Insulin-tolerance tests were performed in 8-week-old mice fed ad libitum. After determination of basal blood glucose concentrations, each mouse received an intraperitoneal injection of insulin (0.75 IU per kg body weight; Novolin R Insulin; Novo Nordisk) and glucose concentrations in blood were measured after 15, 30, 45 and 60 min.
Analysis of body composition. Lean  www.nature.com/scientificreports/ appropriate devices and measured by the built-in automated instruments. Animals were allowed to acclimatize to the cages for at least 12-h before data acquisition for additional 24-h.
Analytical procedure. Blood glucose levels were determined from whole venous blood using an automatic glucose monitor (Bayer Contour, Bayer, Germany). Insulin levels in serum were measured by Cisbio Insulin assay using mouse standards according to manufacturer's guidelines (Cisbio).
Myriocin extraction from Cordyceps. Myriocin was extracted from Cordyceps using a similar protocol described before 71 . Briefly, extraction of myriocin was performed by weighing 0.5 g of dried Cordyceps powder which was then sonicated with 95% methanol (MeOH) (3 × 10 mL) for 40 min at 40 °C. The extract solutions were combined and centrifuged at 10,000 rpm/min for 10 min then evaporated under vacuum. For liquid samples 5 and 7, 10 mL of the liquid extracts were directly used for evaporation. The residue was dissolved in 2 mL of 50% methanol and centrifuged at 12,000 rpm/min for 10 min. The supernatant was then loaded onto an Oasis HLB 3 cc solid-phase extraction (SPE) column (Waters Corp, Milford, MA) pretreated with 10 mL of 100% MeOH, followed by 5 mL of H 2 O. After sample loading, the column was washed with 10% MeOH (1 × 3 mL) and 50% MeOH (1 × 3 mL) successively, and then the myriocin-containing fraction was eluted with 80% methanol (2 × 3 mL). The eluent was evaporated under a gentle stream of nitrogen in a water bath at 50 °C. The residue was then resuspended in 250 µL of 80% MeOH and centrifuged at 12,000 r/min for 10 min. The supernatant was then transferred to a glass vial for LC-MS analysis. Myriocin (M1177, Sigma, St. Louis, MO, USA) was suspended in MeOH to a final concentration of 1 μM to be used as positive control. Lipidomics. Lipid extracts were separated on an Acquity UPLC CSH C18 1.7 µm 2.1 × 50 mm column maintained at 60 °C connected to an Agilent HiP 1290 Sampler, Agilent 1290 Infinity pump, and Agilent 6490 triple quadrupole (QqQ) mass spectrometer. Sphingolipids were detected using dynamic multiple reaction monitoring (dMRM) in positive ion mode. Source gas temperature was set to 210 °C, with a gas (N 2 ) flow of 11 L/min and a nebulizer pressure of 30 psi. Sheath gas temperature was 400 °C, sheath gas (N 2 ) flow of 12 L/min, capillary voltage is 4000 V, nozzle voltage 500 V, high pressure RF 190 V and low-pressure RF was 120 V. Injection volume was 2 µL and the samples were analyzed in a randomized order with the pooled QC sample injection eight times throughout the sample queue. Mobile phase A consisted of ACN: H 2 O (60:40 v/v) in 10 mM ammonium formate and 0.1% formic acid, and mobile phase B consisted of IPA: ACN:H 2 O (90:9:1 v/v) in 10 mM ammonium formate and 0.1% formic acid. The chromatography gradient was started at 15% mobile phase B, increased to 30% B over 1 min, increased to 60% B from 1 to 2 min, increased to 80% B from 2 to 10 min, and increased to 99% B from 10 to 10.2 min where it was held until 14 min. Post-time was 5 min and the flowrate was 0. 35  Determining the composition of gut microbiota using 16s rRNA amplicon sequencing. Microbial profiling was carried out using established protocols in identifying the composition of gut microbiota following dietary treatment 72 . Cecal contents were used to extract genomic DNA using the DNeasy PowerSoil Kit (Qiagen, Md, USA). Fifty nanograms of genomic DNA were used for the amplification of the V4 variable region of the 16S rRNA gene using the 515F/806R primers. Library preparation was performed using the Nextera XT DNA Library Preparation Kit (Illumina, Cat# FC-131-1096) and individual barcodes were labelled with Nextera XT indices to accommodate multiplexing. The Illumina MiSeq platform with of ~ 30% PhiX DNA was used for pooled amplicons, paired-end sequencing (2 × 250 bp) 73 . Standardized pipelines in QIIME 2 were used for the microbial ecology matrices 74 . Alpha diversity metrics for richness, diversity and evenness at the phylum, genus and OTU-levels were determined in QIIME using the observed features, Shannon diversity index and Pielou's evenness index, respectively. Bray-Curtis dissimilarities were used to evaluate beta diversity and to conduct the principal coordinate analysis (PCoA). Spearman's correlations were performed using the Shiny App between bacterial abundance data, body weight, body fat, fasting blood glucose and fed blood glucose were used to deter- www.nature.com/scientificreports/