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Inactivation of various variant types of SARS-CoV-2 by indoor-light-sensitive TiO2-based photocatalyst

Subjects

Abstract

Photocatalysts are promising materials for solid-state antiviral coatings to protect against the spread of pandemic coronavirus disease (COVID-19). This paper reports that copper oxide nanoclusters grafted with titanium dioxide (CuxO/TiO2) inactivated the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus, including its Delta variant, even under dark condition, and further inactivated it under illumination with a white fluorescent bulb. To investigate its inactivation mechanism, the denaturation of spike proteins of SARS-CoV-2 was examined by sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE) and enzyme-linked immunosorbent assay (ELISA). In addition to spike proteins, fragmentation of ribonucleic acids in SARS-CoV-2 was investigated by real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR). As a result, both spike proteins and RNAs in the SARS-CoV-2 virus were damaged by the CuxO/TiO2 photocatalyst even under dark condition and were further damaged under white fluorescent bulb illumination. Based on the present antiviral mechanism, the CuxO/TiO2 photocatalyst will be effective in inactivating other potential mutant strains of SARS-CoV-2. The CuxO/TiO2 photocatalyst can thus be used to reduce the infectious risk of COVID-19 in an indoor environment, where light illumination is turned on during the day and off during the night.

Introduction

The novel coronavirus disease (COVID-19) has been breaking out since 20191,2. This pandemic scale disease is caused by the infection of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus, mainly through airborne transmission of droplets and/or aerosols produced by infected persons3. In addition to the risk of person-to-person contact, active the SARS-CoV-2 viruses have also been detected on the surfaces of objects in public places like hospitals4. Therefore, antiviral chemicals and/or materials are useful for protecting against the spread of SARS-CoV-2. Indeed, alcohol5, hydrogen peroxide6, and hypochlorous acid7 have been widely used to inactivate bacteria and/or viruses on surfaces of various objects, such as tables, floors, handrails, touch panels/buttons, and furniture. These chemicals inactivate viruses by denaturing their proteins8, however, their antiviral effect is not sustained over the long term because of their evaporation and/or dissipation. Conversely, solid-state antiviral compounds are useful because of their robustness and feasibility for use as coating materials9. For example, Ito et al.10 reported the inactivation of SARS-CoV-2 by cerium molybdates nanoparticles. Among these various solid-state antibacterial and antiviral materials, titanium dioxide (TiO2)-based photocatalysts are promising because of their non-toxic, economical (abundant), chemically and/or thermally stable properties11,12,13, and their antiviral effect can be continuously maintained by illuminating ultraviolet (UV) light14,15,16. Photogenerated holes in the valence band of TiO2 have strong oxidation power for decomposing organic molecules; thus, viral components such as surface proteins can be oxidised under UV irradiation, resulting in virus inactivation. Recently, TiO2 has been reported to inactivate SARS-CoV-2 through photocatalytic process17,18; however, TiO2 can only function under UV light, which is hardly contained in a normal room lighting apparatus. Because viral infections mainly occur in indoor environments where many people gather, it is necessary to use a visible-light-sensitive antiviral photocatalyst. It is also noted that lighting is usually turned off during the night; thus, the sustained antiviral property of photocatalysts under dark conditions is also an important requirement for its practical use.

In the present study, we focus on the combination of TiO2 with copper oxide as an antiviral composite, although both of them are not catalytically active under dark or visible-light irradiation19,20,21. According to the previous experimental studies, both TiO2 and CuO do not so inactivate bacteria well under the dark condition19,20. Under UV light irradiation, TiO2 can inactivate viruses and/or bacteria20, whereas CuO is not photocatalytically active because of its shallow valence band21. In other words, neither TiO2 nor CuO works well under dark and/or visible light irradiation. In the present study, however, there are two key points for the development of an indoor-active antiviral material. First, we combine TiO2 with CuO for visible-light sensitivity by the interfacial charge transfer mechanism22,23. Second, we introduce Cu(I) species in CuO resulting CuxO nanoclusters to achieve the efficient antiviral property even under dark, since Cu(I) species work as active sites for antibacterial and antiviral functions20,24,25. Recently, our group has developed a visible-light-sensitive antiviral/antibacterial photocatalyst based on copper oxide (CuxO, 1 < x < 2) nanocluster-grafted TiO225,26,27. CuxO nanoclusters are composed of a mixed valence number oxide, in which Cu(I) and Cu(II) species are present. The Cu(II) species in CuxO contributes to the visible-light-driven photocatalysis reaction, whereas the Cu(I) species plays a crucial role in denaturing virus proteins, thereby causing their inactivation under dark conditions. However, these previous studies examined their antiviral properties using bacteriophage Qβ without an envelope, which is a different structure from that of the SARS-CoV-2 (with an envelope). It is worthy to investigate the photocatalytic antiviral property against the not only wild-type strain but also various variant types of SARS-CoV-2 with deep antiviral mechanism study. For example, Delta variant of SARS-CoV-2 spreads faster than the wild-type strain28, and mutated Omicron variant recently emerges so far with enhanced transmissibility29,30.

Herein, we report the inactivation of the SARS-CoV-2 virus by the CuxO/TiO2 photocatalyst and even under dark condition; further, its efficient anti-SARS-CoV-2 performance is achieved under a white fluorescent bulb passed through a UV cutoff filter. The CuxO/TiO2 photocatalyst inactivated not only the wild-type strain but also the Alpha, Beta, Gamma, and Delta variants. Since the spike proteins play an important role in SARS-CoV-2 infection, the present study carefully examines the denaturation of spike proteins in SARS-CoV-2 virus by sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE) and enzyme-linked immunosorbent assay (ELISA) analyses31. The SARS-CoV-2 virus has an outer envelope, which involves haphazardly arranged spike proteins that are key to fusing with human cells32. In addition to spike proteins, ribonucleic acids (RNAs) in viruses are essential for generating their copies in human cells33. Thus, the present study also analyses the defragmentation of RNAs in SARS-CoV-2 by the CuxO/TiO2 photocatalyst using a real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR) technique. We carefully discuss the mechanism of the inactivation of SARS-CoV-2 by the CuxO/TiO2 photocatalyst under dark and light illumination conditions.

Results

Characterisation and photocatalytic activity of CuxO/TiO2

The CuxO nanoclusters were facilely grafted onto TiO2 powder by a simple impregnation method based on a previous report25. Briefly, rutile TiO2 powder was dispersed in an aqueous solution of copper chloride (CuCl2) under stirring at 90 °C, and then sodium hydroxide and glucose were added to reduce Cu(II) species to Cu(I) species. After washing, filtration, and drying, CuxO/TiO2 powder was obtained. For antiviral evaluation, CuxO/TiO2 powder was coated onto a glass substrate.

Figure 1a shows a transmission electron microscopy (TEM) image of CuxO/TiO2. The size of the TiO2 powder ranged from approximately 100 to 200 nm, on which small CuxO nanoclusters of a few nanometres in size were grafted. Our energy dispersive X-ray spectrometer (EDS) equipped in a TEM apparatus, scanning electron microscope (SEM) image with its EDS mapping, and X-ray diffraction (XRD) pattern indicated that the grafted small nanoclusters were composed of amorphous copper oxide (see Supplementary Fig. 1–3).

Figure 1
figure 1

Characterization of CuxO/TiO2. (a) TEM image of CuxO/TiO2 and (b) UV–Vis spectra of CuxO/TiO2 (red line) and pristine TiO2 (black line). Vertical axis of absorption spectra was Kubelka–Munk function, based on the raw data of diffuse reflectance spectra.

Figure 1b shows the ultraviolet–visible (UV–Vis) absorption spectra of CuxO/TiO2 and bare TiO2. Bare TiO2 absorbs UV light below 400 nm, which is attributed to the band-to-band transition in TiO2. On the other hand, CuxO/TiO2 exhibits a broad visible light band. The absorption band in the range of 400–500 nm is assigned to interfacial charge transfer (IFCT) from the valence band of TiO2 to the unoccupied orbital of Cu(II) species22,23, which was confirmed by our in situ electron spin resonance (ESR) analysis under light irradiation (see Supplementary Fig. 4). In addition, the absorption in the range of 500–600 nm originates from the band-to-band transition in CuxO, while the absorption over 650 nm is assigned to the d–d transition of the Cu(II) species34. According to a previous report, IFCT transition induced by blue light (around 400–500 nm) irradiation only contributed to the photocatalytic oxidation activity among the observed visible-light absorption bands25. Indeed, the present CuxO/TiO2 exhibited photocatalytic activity under blue light, while the pristine TiO2 did not show any activity under the same blue light (see Supplementary Fig. 5a). The CuxO/TiO2 photocatalyst could completely oxidise gaseous 2-propanol molecules into carbon dioxide under visible-light irradiation (Supplementary Fig. 5b). On the basis of its strong oxidising power, the CuxO/TiO2 photocatalyst is expected to have high antiviral function. Furthermore, its antiviral activity under dark condition was examined and discussed below.

Inactivation of SARS-CoV-2

The photocatalytic antiviral activity of CuxO/TiO2 against SARS-CoV-2 virus was evaluated by the method reported in our previous study15. Figure 2a shows the inactivation properties of the SARS-CoV-2 virus of wild-type strain under dark and visible light irradiation conditions. Visible light irradiation was conducted using a commercial white fluorescence bulb (see its spectrum in Supplementary Fig. 6), which is usually used as an indoor lighting apparatus. For light irradiation, the UV region was cut off by inserting an optical filter, and the light intensity was set at 1000 lx. We investigated the antiviral properties of the glass substrate without photocatalyst as a control group and found that the virus titer on the glass did not decrease under dark and visible light irradiation conditions. In contrast, the virus titer on the CuxO/TiO2 photocatalyst drastically decreased even under dark condition. After 3 h of exposure to CuxO/TiO2 in the dark, the virus titer decreased to the detection limit. Furthermore, the antiviral property of the photocatalyst was improved under visible light irradiation, and the virus titer decreased to the detection limit after only 2 h. In other words, four orders of magnitude of the virus were inactivated by the CuxO/TiO2 photocatalyst even after 2 h under regular indoor lighting. Figure 2b,c show photographs of SARS-CoV-2 plaques for the glass substrate and CuxO/TiO2 photocatalyst under visible light irradiation, where white spots indicate active SARS-CoV-2 viruses. These photos also demonstrate the efficient photocatalytic antiviral properties of our CuxO/TiO2 photocatalyst.

Figure 2
figure 2

Inactivation of wild-type strain of SARS-CoV-2 by photocatalyst. (a) changes of virus titer of SARS-CoV-2 (wild-type strain, 2019-nCoV JPN/TY/WK-521) for each condition, (b) photograph of viral plaques infected by SARS-CoV-2 for a bare glass substrate under visible light irradiation, and (c) photograph of viral plaques infected by SARS-CoV-2 for CuxO/TiO2 photocatalyst under visible light irradiation. White spots in photos indicate the active SARS-CoV-2 virus. Visible light irradiation was conducted using a white fluorescence bulb passed through a UV cutoff filter with the light intensity of 1000 lx. Asterisk marks (*) and dashed line in the panel (a) indicate detection limit of the virus.

In addition to the wild-type strain of SARS-CoV-2, we investigated the inactivation properties of CuxO/TiO2 versus Alfa (α), Beta (β), Gamma (γ), and Delta (δ) variants. Figure 3a shows the virus titer of Alfa, Beta, and Gamma variants on bare glass and those on the CuxO/TiO2 coated sample. The virus titer was examined before light irradiation (dark) and after visible light irradiation for 2 h. Similar to the wild-type strain of SARS-CoV-2 shown in Fig. 2, the CuxO/TiO2 disinfected four orders of various variants under visible light irradiation. Figure 3b shows the disinfection of the Delta variant, which is recently causing critical surges in various countries28. It is noteworthy that the virus titer of the Delta variant decreased to the detection limit only after 2 h of exposure to the CuxO/TiO2 photocatalyst under visible light irradiation and after 3 h of exposure under dark condition.

Figure 3
figure 3

Inactivation of various types of variants. (a) changes of virus titer of Alfa (α), Beta (β), and Gamma (γ) variants under 0 and 2 h of visible light irradiation and (b) changes of virus titer of Delta (δ) variant. Visible light irradiation was conducted using a white fluorescence bulb passed through a UV cutoff filter with the light intensity of 1000 lx. Asterisk marks (*) and dashed lines in the panels indicate detection limit of viruses.

Previously, Uema et al. reported the anti-SARS-CoV-2 properties of a tungsten trioxide (WO3)-based photocatalyst35, but it did not work well under dark conditions. In this report, the virus titer was reduced by only two orders of magnitude after 3 h under 3000 lx white light irradiation, which was much stronger than the present illumination conditions. TiO2 based photocatalyst used in this study has an advantage over these previous studies because of its more efficient anti-SARS-CoV-2 property under visible light irradiation. In addition, our TiO2 based material is more chemically stable under alkaline and acidic conditions and is more economical than WO3 based photocatalysts. Furthermore, the present CuxO/TiO2 exhibits an efficient antiviral activity even in the dark condition, while most of the reported photocatalysts were not functioned under the dark. We previously investigated the antiviral mechanism of CuxO/TiO2 using bacteriophage Qβ without an envelope as a virus. It is well understood that the organic substances in bacteriophage Qβ are oxidised to carbon dioxide, causing inactivation of viruses under visible light irradiation. In addition, Cu(I) species in CuxO nanoclusters cause denaturation of protein even in dark condition by the strong adsorption between protein and CuxO27. Sunada et al.20,24 investigated the antiviral mechanism of copper oxides and found that the inactivation property of Cu(I) species was due to its solid-state property toward strong protein adsorption, rather than by the generation of reactive oxygen species or leached copper ions. These previous studies used bacteriophage Qβ without envelope for their mechanism studies; thus, we carefully investigated the denaturation of both spike proteins and ribonucleic acids (RNAs) in SARS-CoV-2 by using SDS-PAGE, ELISA, and RT-qPCR analyses, as presented in the next section.

Damage of spike proteins and RNAs in SARS-CoV-2 viruses

Previous studies have reported that coronavirus-spike (S) glycoproteins promote entry into cells and are the main target of antibodies31. Figure 4a,b show the results of SDS-PAGE analysis for CuxO/TiO2 and bare glass under dark condition (a) and under visible light irradiation condition (b). It is noteworthy that the band signal of S1 proteins and receptor binding domain (RBD) at S1 proteins were obviously decreased after exposure to the CuxO/TiO2 catalyst under dark conditions as well as under visible light irradiation. As RBD recognizes ACE2, a receptor on the surface of host cells36,37, the signal disappearance of RBD suggests a decrease in virus infectivity. We also quantitatively evaluated the denaturation of S1 spike proteins by ELISA, and the results are shown in Fig. 4c. The spike proteins were not denatured on a control glass substrate under dark and light illumination conditions. On the other hand, the spike proteins were denatured on the CuxO/TiO2 photocatalyst even in the dark, and its denaturation property was further enhanced by visible light irradiation. These results indicate that the CuxO/TiO2 photocatalyst denaturalises S1 spike proteins, which play an essential role in entry into lung cells, even under dark conditions as well as under visible light irradiation. Previously, similar denaturation properties to albumin, haemagglutinin, and neuraminidase were observed by Cu(I) species in copper oxides20,24. According to these previous studies, the strong antiviral ability of Cu(I) species is owing to its strong adsorption ability towards proteins, rather than the effects of reactive oxygen species or leached copper ions. Therefore, the efficient antiviral activity of CuxO/TiO2 photocatalyst even under the dark condition is attributed to its strong adsorption property yielding denaturation of proteins. Protein molecules were further oxidised by the CuxO/TiO2 photocatalyst under visible light illumination, because the photogenerated holes in the valence band of TiO2 generated through the interfacial charge transfer have strong oxidative power for complete decomposition into carbon dioxide molecules, as shown in the Supplementary Information (Fig. 5).

Figure 4
figure 4

Analysis of SARS-CoV-2 proteins by SDS-PAGE and ELISA. SARS-CoV-2 proteins under (a) dark condition and (b) visible light irradiation condition for glass substrate and CuxO/TiO2 photocatalyst were separated by SDS-PAGE. Lanes 0 h, 1 h, 2 h, 3 h, 4 h, 6 h, and 8 h corresponded to the time after the reaction, respectively. ELISA results (c) on SARS-CoV-2 spike S1 protein concentration under dark condition and visible light irradiation condition for glass substrate and CuxO/TiO2 photocatalyst. Visible light irradiation was conducted using a white fluorescence bulb passed through a UV cutoff filter with the light intensity of 1000 lx.

Figure 5
figure 5

RT-qPCR analysis for SARS-CoV-2 N gene. Changes in RNA copies on glass and CuxO/TiO2 photocatalyst under visible light irradiation or dark condition. Visible light irradiation was conducted using a white fluorescence bulb passed through a UV cutoff filter with the light intensity of 1000 lx.

In addition to spike protein denaturation, we also investigated the fragmentation of RNA in the SARS-CoV-2 virus under exposure to our catalyst. Figure 5 shows the changes in the RNA copies of SARS-CoV-2. Similar to the trends of virus titer and spike proteins shown above, RNA copies were also decreased by exposure to the CuxO/TiO2 catalyst, even under dark condition. Furthermore, visible light irradiation of the CuxO/TiO2 photocatalyst enhanced the fragmentation of RNAs in SARS-CoV-2. Based on these results, the fragmentation of RNA by the present photocatalyst also contributes to its efficient antiviral activity. The present CuxO/TiO2 photocatalyst causes damage to both spike proteins and RNAs in SARS-CoV-2, yielding efficient inactivation under dark condition as well as under indoor light illumination. The change in the number of RNA copies on the CuxO/TiO2 photocatalyst under visible light irradiation exhibited a two-step reduction trend (Fig. 5). The initial reduction step (0–4 h) would proceed by similar fragmentation of RNAs under the dark condition. Previously, Qiu et al. investigated the degradation of supercoiled plasmid pBR322 DNA by the present Cu(I) species in CuxO, and they found that the plasmid DNA from the supercoiled to the open circular form was clearly observed by the exposure to Cu(I) species in CuxO even under the dark condition25. Therefore, we speculate that the strong adsorption ability of Cu(I) species also causes fragmentation of RNAs even under the dark condition. On the other hand, the second step reduction (4–8 h) would be further driven by the photocatalytic oxidation process, which is able to oxidise organic molecules into water and carbon dioxide molecules. However, we would like to emphasise that complete oxidation is not necessary to inactivate the SARS-CoV-2 virus to the detection limit. As shown in Figs. 2 and 3, an exposure time of 3 h was sufficient for CuxO/TiO2 to inactivate SARS-CoV-2 below the detection limit, and even under dark condition. These results indicate that the 3 h exposure to CuxO/TiO2 denaturalises spike proteins and also causes fragmentation of RNA, as proven by our SDS-PAGE, ELISA, and RT-qPCR analyses.

Figure 6 shows the antiviral mechanism of the CuxO/TiO2 photocatalyst. The Cu(I) species in CuxO denaturalises spike proteins and also causes RNA fragmentation of SARS-CoV-2, even under dark condition, yielding inactivation under the detection limit after only 3 h. Furthermore, light irradiation causes the photocatalytic oxidation of the organic molecules of SARS-CoV-2. Based on this antiviral mechanism, involving denaturation of proteins, fragmentation of RNAs, and oxidation of organic substances by photocatalysis, the CuxO/TiO2 photocatalyst is not limited to a specific variant of the virus. It will be effective to inactivate other types of a mutant strain of SARS-CoV-2, such as Omicron strain29. It is noted that in the case of vaccines and/or oral drugs, there is a possibility that resistant mutants will emerge in the future. In contrast to vaccines or drugs, the CuxO/TiO2 photocatalyst is very useful because it has potential effectiveness against various mutants broadly.

Figure 6
figure 6

Speculated antiviral mechanism of CuxO/TiO2.

The present study mainly focused on the inactivation of SARS-CoV-2 which consists of an envelope membrane. We also investigated the antiviral properties of feline calicivirus (FCV), which does not have an outer envelope, in contrast to SARS-CoV-2 (see Supplementary Fig. 7). Notably, the present CuxO/TiO2 catalyst also inactivated FCVs even under the dark condition, and its performance was further improved under visible-light irradiation. Our previous studies also revealed that the CuxO/TiO2 inactivated bacteriophage Qβ, which does not have an envelope membrane25,27. These results strongly indicate that the CuxO/TiO2 photocatalyst is effective for inactivation of various kinds of viruses by its denaturation and/or strong oxidation ability. Furthermore, CuxO/TiO2 exhibited a significant antibacterial effect on Escherichia coli and Staphylococcus aureus, as well as viruses25. Thus, the CuxO/TiO2 will be one of the valuable anti-microorganism materials with wide spectrum.

It is important to discuss the toxicity of the present CuxO/TiO2 for its practical use. It has been reported the cytotoxic risk of TiO2 and CuO nanoparticles towards Zebrafish or colon cells38,39,40. On the other hand, the previous studies directly examined the influence of TiO2 and CuO particles on human skin41,42. These papers concluded that the TiO2 and CuO have extremely low risk to human skin. In the present study, we have conducted the Salmonella reverse mutation assay test (Ames test)43 on CuxO/TiO2 to discuss its genotoxic risk (Supplementary Material, Table 1 and 2). The test results indicate its extremely low risk. We suppose that the present antiviral particles will be mainly applied as a solid-state coating material on a substrate. Under such applications, concentrated particles are not exposed to human for long term. Therefore, we expect that our antiviral material can be safely used for various coating applications.

Conclusion

The CuxO/TiO2 photocatalyst inactivated SARS-CoV-2 even under dark condition, and its antiviral performance was improved by white light illumination, which is usually used as an indoor light apparatus. Thus, the antiviral function of the CuxO/TiO2 photocatalyst can be maintained in an indoor atmosphere, where light illumination is turned on during the day and off during the night without any maintenance, such as spraying of antiviral liquid or wipe-off procedures. The present photocatalyst denaturalises spike proteins and also causes fragmentation of RNAs in the SARS-CoV-2 virus, as proven by SDS-PAGES, ELISA, and RT-qPCR analyses. The CuxO/TiO2 photocatalyst has already been commercialised (NAKA CORPORATION, Tokyo Japan) and is expected to be applied to various antiviral industrial items in indoor environments, such as hospitals, airports, metro stations, and schools, as coating materials for air filters, respiratory face masks, and antifungal fabrics to prevent the COVID-19 spread.

Methods

Synthesis of CuxO/TiO2 powder and film

The CuxO nanoclusters were grafted onto rutile TiO2 powder using an impregnation technique. In a typical preparation, one gram of TiO2 was dispersed in 10 mL of aqueous CuCl2 solution in a vial reactor. The weight fraction of Cu relative to TiO2 was 0.25%. During stirring, the temperature of the aqueous solution was maintained at 90 °C for 1 h. Then, sodium hydroxide (NaOH) and glucose were added to the solution (molar ratio of NaOH/glucose/CuCl2 = 8/4/1) at the same temperature to graft CuxO nanoclusters onto TiO2 particles25.

The CuxO/TiO2 powder was suspended in 99% ethanol at a concentration of 1 mg/ml. Then, 0.6 ml of the suspension was uniformly loaded onto a soda-lime glass substrate (50 mm × 50 mm) and dried at 100 °C for 15 min. By repeating this operation twice, the glass substrate was thus coated with CuxO/TiO2 powder (1.2 mg).

Characterisation of photocatalyst

Transmission electron microscopy (TEM) images were obtained using a JEM-2100F TEM/STEM (JEOL, Japan) operated at an acceleration electron beam voltage of 200 kV. Field-emission scanning electron microscopy (FE-SEM) images and energy-dispersive X-ray (EDS) analysis were performed using an S4700 (Hitachi High-Tech, Japan). X-ray diffraction (XRD) patterns were recorded using a SmartLab diffractometer (Rigaku, Japan) with Cu Kα radiation (λ = 1.5418 Å). A Si non-reflective plate was used as the substrate. UV–visible (UV–Vis) diffuse reflectance spectra were recorded using a spectrophotometer (V-670, JASCO, Japan) equipped with an integration sphere unit. Optical absorption spectra were obtained using the Kubelka–Munk function44 calculated from raw reflection data, where a white BaSO4 plate was used as the reflectance standard. Electron spin resonance (ESR) spectra were recorded using an in situ ESR system under light irradiation (EMX Nano, Bruker). For ESR measurements, the photocatalyst powder was placed into a quartz tube filled with nitrogen gas at 90 K with a microwave frequency (X-band) of 9.629 GHz to 9.633 GHz.

Photocatalytic oxidation activity test

The photocatalytic oxidation activity was evaluated by monitoring the oxidation of gaseous 2-propanol into acetone and carbon dioxide under visible-light irradiation, as the oxidation pathway was well studied in a previous paper45. A 100 mg CuxO/TiO2 sample was uniformly spread over a glass dish (5.5 cm2). Before each photocatalysis test, pre-irradiation was performed overnight in a 500 mL reaction vessel filled with fresh synthetic air to eliminate organic contaminants on the sample surface. Next, the gas inside the reactor was replaced with fresh synthetic air, and 4.1 μmol gaseous 2-propanol was injected into the reactor. Before light irradiation, the system was kept in the dark for 1 h to allow 2-propanol gas to reach absorption equilibrium. Visible light irradiation was performed using a blue light emission diode (LED) with an intensity of 20 mW/cm2 measured using a spectro-radiometer (USR-40D, Ushio, Japan), which could drive the interfacial charge transfer (IFCT) in CuxO/TiO2. Furthermore, a relatively higher intensity of visible light source (85 mW/cm2 by a 150 W Xe lamp with a UV cutoff filter below 420 nm) was also used to investigate whether our photocatalyst could completely oxidise 2-propanol molecules to carbon dioxide. Concentrations of 2-propanol and produced gases of acetone and carbon dioxide were measured using a photoacoustic gas monitor (1412i, INNOVA).

Virus strain

The SARS-CoV-2 virus reference strains used in this study were the wild-type strain 2019-nCoV JPN/TY/WK-521 and variants of concern (VOCs), including the Alpha variant (B.1.1.7) strain hCoV-19/Japan/QHN001/2020, Beta variant (B.1.351) strain hCoV-19/Japan/TY8-612/2021, Gamma variant (B.1.617.2) strain hCoV-19/Japan/TY7-501/2021, and Delta variant (P.1) strain hCoV-19/Japan/TY11-927-P1/2021, which were isolated and supplied by National Institute of Infectious Disease, Tokyo, Japan, to evaluate the antiviral activity of photocatalysis. These variants were propagated in the African green monkey kidney epithelial cells (Vero E6/TMPRSS2, purchased from Japanese Collection of Research Biosources Cell Bank, National Institute of Biomedical Innovation) which were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Gibco, USA) supplemented with 10% foetal bovine serum (FBS) at 37 °C. Viral titer was determined by the plaque assay technique on confluent layers of Vero E6/TMPRSS2 cell cultures grown in 12-well culture plates as described previously46,47. Plaques were quantified and recorded as plaque-forming units (PFU)/mL. All experiments were repeated three separate times, and the average titer was determined; the plaque assay was duplicated in each test. All infection experiments were performed in a biosafety level-3 (BLS-3) laboratory.

Analysis of CuxO/TiO2 inactivation of SARS-CoV-2 virus

The photocatalytic antiviral activity of CuxO/TiO2 against SARS-CoV-2 virus was determined according to ISO 18071:2016 (Fine Ceramics [Advanced Ceramics, Advanced Technical Ceramics]—Determination of antiviral activity of semiconducting photocatalytic materials under indoor lighting environment—Test method using bacteriophage Q-beta) and JIS R 1756 with minor modification from our previous study15. All experiments were performed in a light-tight box to prevent any influence of indoor light and sunlight. The viral suspension (100 μL) was dropped onto a CuxO/TiO2-coated glass plate (50 mm × 50 mm) or non-coated glass as the control and was spread out by covering with an adhesive film (40 mm × 40 mm). The sample was then illuminated with visible light irradiation or under dark condition. Overhead illumination by visible light was provided by using a tubular white fluorescent lamp (FL20SSW/18; Toshiba, Japan) with a UV cutoff filter below 400 nm. The light intensity reaching the surface at the centre of the glass reaction vessel was adjusted to 1000 lx by changing the horizontal distance between the samples and the lamp using a digital illuminance meter IM-5 (TOPCON, Japan). After the reaction time, the virus was collected with 5 mL of phosphate buffered saline (PBS; Sigma-Aldrich Corp., USA) solution, and the virus titer was determined by plaque assay.

Analysis of SARS-CoV-2 protein on CuxO/TiO2 catalyst (SDS-PAGE and ELISA)

After being illuminated with CuxO/TiO2-coated glass or control glass for a certain period (0–8 h), all samples were collected and quantitative alterations of the proteins were analysed by sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE) and enzyme-linked immunosorbent assay (ELISA). For the SDS-PAGE analysis, collected samples were extracted by EzApply (ATTO, Japan) and the protein of the SARS-CoV-2 virus was separated by 10% SDS–polyacrylamide gels as previously described48. Protein bands were stained with SYPRO Ruby (Thermo Fisher Scientific, USA) and visualised using a Chemidoc imaging system (BioRad, France).

The quantification of SARS-CoV-2 spike S1 protein of the collected sample was determined using a SARS-CoV-2 Spike S1 Protein ELISA Kit (RK04154; ABclonal, USA), according to the manufacturer's instructions. The calibration standards were assayed at the same time as the samples and allowed the operator to produce a standard curve of optical density versus SARS-CoV-2 spike S1 protein concentration. The concentration of the samples was then determined by comparing the O.D. of the samples to the standard curve. Absorbance was measured at 450 nm using a spectrophotometer. The samples were tested in duplicates.

Detection of SARS-CoV-2 N gene by RT-qPCR

The quantity of viral RNA was analysed using RT-qPCR. Briefly, RNA was extracted from the samples using the QIAamp Viral RNA Mini Kit (QIAGEN, Hilden, Germany), according to the manufacturer’s instructions. RT-qPCR was performed using the QuantiTect Probe RT-PCR Kit (QIAGEN) on the QuantStudio 5 Real-Time PCR System (ThermoFisher, USA) and the following set of primers/probes specific for the viral N gene: The forward primer, 5’-AAATTTTGGGGACCAGGAAC-3’; the reverse primer, 5’-TGGCAGCTGTGTAGGTCAAC-3’; and the probe, 5’-(FAM) ATGTCGCGCATTGGCATGGA (BHQ)-3′49. The cycle threshold (Ct) values of RT-qPCR were converted into viral RNA copy numbers based on a standard curve prepared from tenfold serial dilutions of known copy numbers of SARS-CoV-2 RNA.

Data availability

The data that support the findings of this study are available from the article and Supplementary Information files, or from the corresponding authors upon reasonable request.

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Acknowledgements

This work was funded by the New Energy and Industrial Technology Development Organization (NEDO) in Japan (project name: Project to Create Photocatalyst Industry for Recycling-Oriented Society). This NEDO project was directed by Prof. K. Hashimoto at the University of Tokyo, Japan (now president of the National Institute for Materials Science [NIMS], Japan). We are also thankful to the project members of NEDO, including Prof. H. Irie at Yamanashi University, Japan; Dr. M. Minoshima at Osaka University, Japan; Dr. Y. Kuroda at Showa Denko K.K., Japan; Prof. H. Yu at Wuhan University of Technology, China; Prof. X. Qiu and Prof. M. Liu at Central South University, China; and other collaborators for their great help in the development of the present photocatalyst. We also thank Mr. A. Genseki at Open Facility Center, Tokyo Institute of Technology for the TEM observation. This research was also financially supported by the NAKA Corporation (Tokyo) and JSPS Kakenhi (18H02055). Finally, we would like to thank Editage (www.editage.com) for English language editing.

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R.N., A.N., Y.S., and H.Y. carried out experimental studies on the inactivation of SARS-CoV-2 and RNA/protein denaturation. K.S., T.N., and H.I. conducted experimental studies on the anti-viral properties of photocatalysts. A.Y. and M.M. developed the photocatalyst materials and characterised their structures. R.N., H.I., and M.M. conceived the project. M.M. wrote the first draft of this paper, and all authors discussed the results and organisation of the manuscript.

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Correspondence to Ryuichi Nakano, Hitoshi Ishiguro or Masahiro Miyauchi.

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Nakano, R., Yamaguchi, A., Sunada, K. et al. Inactivation of various variant types of SARS-CoV-2 by indoor-light-sensitive TiO2-based photocatalyst. Sci Rep 12, 5804 (2022). https://doi.org/10.1038/s41598-022-09402-7

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