Inhibition of triple negative breast cancer-associated inflammation, tumor growth and brain colonization by targeting monoacylglycerol lipase

While the prevalence of breast cancer metastasis in the brain is significantly higher in triple negative breast cancers (TNBCs), there is a lack of novel and/or improved therapies for these patients. Monoacylglycerol lipase (MAGL) is a hydrolase involved in lipid metabolism that catalyzes the degradation of 2-arachidonoylglycerol (2-AG) linked to generation of pro- and anti-inflammatory molecules. Here, we targeted MAGL in TNBCs, using a potent carbamate-based inhibitor AM9928 (hMAGL IC50 = 9 nM) with prolonged pharmacodynamic effects (46 h of target residence time). AM9928 blocked TNBC cell adhesion and transmigration across human brain microvascular endothelial cells (HBMECs) in 3D co-cultures. In addition, AM9928 inhibited the secretion of IL-6, IL-8, and VEGF-A from TNBC cells. TNBC-derived exosomes activated HBMECs resulting in secretion of elevated levels of IL-8 and VEGF, which were inhibited by AM9928. Using in vivo studies of syngeneic GFP-4T1-BrM5 mammary tumor cells, AM9928 inhibited tumor growth in the mammary fat pads and attenuated blood brain barrier (BBB) permeability changes, resulting in reduced TNBC colonization in brain. Together, these results support the potential clinical application of MAGL inhibitors as novel treatments for TNBC.

Breast cancer is a common cause of brain metastases occurring in at least 10-16% of patients and reaching 25-35% of TNBC patients [1][2][3][4][5] . Unfortunately, patients who are diagnosed with brain metastases often have poor prognosis with short overall survival times 6 . Triple negative breast cancer (TNBC) more frequently affects younger patients and have higher prevalence in African-American and Hispanic women 6,7 . TNBC tumors are larger in size and more biologically aggressive with lymph node involvement. TNBC patients often have a higher rate of distant recurrence and a poorer prognosis than patients with other breast cancer subtypes 8 . Breast cancer metastases in brain (BCM/B) show significant morphological and genomic heterogeneity [1][2][3][4][5][6][9][10][11][12][13] . Patients with BCM/B have high mortality resulted from the brain lesions and are resistant to chemotherapy treatments 9,10 . Metastatic breast tumor cells transmigrate across the blood-brain barrier (BBB) and form colonies of tumor cells in brain 4,9,10 . Under normal conditions, the BBB is a highly selective barrier due to existence of tight junctions (TJs) between adjacent brain microvascular endothelial cells (BMECs). However, in the process of invasion of tumor cells to the brain, inflammatory cytokines and chemokines secreted by these infiltrating tumor cells disrupt the BBB integrity. Although the BBB prevents the delivery of most therapeutic drugs into the brain, the circulating metastatic tumor cells invade the damaged BBB to form colonies in the brain 9,10 .
Several genes were shown to be involved in the development of brain metastases which include cyclooxygenase COX-2, EGFR ligand HBEGF and α-2,6-sialyltransferase ST6GALNAC5 9,14 , all involved in facilitating cancer cell passage through the BBB. We have previously reported the roles of the proinflammatory peptide P in impairing the BBB integrity and the angiogenic factor Angiopoietin-2 in mediating activation of brain microvascular endothelial cells and BBB impairment, resulting in infiltration of TNBCs into the brain 15,16 . Further, loss of E-cadherin was found in breast cancer metastasis and its expression inversely correlates with tumor stage, pathologic stage, and prognosis of cancers of epithelial origin 17 .
The tumor microenvironment (TME) is important in cancer progression [10][11][12] . TME is characterized by chronic inflammation which stimulates tumorigenesis, especially in inflammatory breast cancer, where metastasis arises at the initial stage. TME is comprised of cells surrounding the tumor (such as macrophages, fibroblasts, and endothelial cells) and the extracellular matrix (ECM). TME in tumor niches differs from the healthy tissue microenvironments in cell type composition and phenotype [10][11][12] . TME promotes tumor development by complex signaling molecules that include soluble secreted molecules such as cytokines, chemokines, growth factors, proinflammatory enzymes, exosomes and matrix remodeling proteinases [10][11][12][13]18 . Interestingly, extracellular vesicles, a heterogeneous group of cell-derived membranous structures comprising of exosomes and microvesicles, were shown recently to breach intact BBB via Transcytosis 19 . Although tumor-derived exosomes are being recognized as essential mediators of intercellular communication between cancer and immune cells, it is not known whether exosomes derived from breast cancer cells can activate directly brain endothelial cells.
Cancer cells endogenously synthesize 95% of free fatty acids (FFAs) de novo 4 and incorporate and remodel exogenous palmitate into structural and oncogenic glycerophospholipids, sphingolipids and ether lipids 4 . Fatty acid synthesis (FAS) pathway confers a survival advantage to cancer cells and especially to tumor cells that are resistant to chemotherapy 4 . Fatty acid-binding protein 5 (FABP5) promoted lipolysis droplets, de-novo fatty acid synthesis, and coordinated lipid signaling that promoted prostate cancer metastasis 20 . Increased FFA levels in tumors lead to enhanced tumor aggressiveness by increasing lipid synthesis mediated by lipolytic pathways 4,21-23 . Thus, targeting lipid metabolism may prevent the gain of survival advantage and improve treatment response in cancer cells.
MAGL is a serine hydrolase that regulates a fatty acid network that promotes cancer pathogenesis by enriching pro-tumorigenic signaling molecules. MAGL is a key hydrolytic enzyme in the FFA tumor network reported in colorectal cancer, neuroblastoma, nasopharyngeal carcinoma, and other cancers 19,[21][22][23] . MAGL primarily hydrolyzes endocannabinoid 2-AG and other monoacylglycerides, and accounts for about 85% of 2-AG degradation, while the remaining part is degraded by α/β-hydrolase domain containing (ABHD) 6/12 enzymes. In addition, MAGL indirectly controls the levels of free fatty acids derived by their hydrolysis and other lipids derived by metabolism of fatty acids with pro-inflammatory or pro-tumorigenic effects 23 . Recently, 2-AG was shown to be the source for arachidonic acid, the precursor of prostaglandins and other inflammatory mediators [24][25][26] . Accumulated studies showed that MAGL inhibition impaired cell migration, invasion and tumorigenicity in some types of cancers [20][21][22][23] . Further, MAGL promoted progression of hepatocellular carcinoma via NF-kB mediated epithelial-mesenchymal transition 27 . Taken together, although MAGL was shown to be involved in some cancers and inflammatory diseases, its specific roles in TNBC tumor progression and the brain metastasis process is still unknown.
A new series of highly potent carbamate MAGL inhibitors were synthesized and characterized at the Center for Drug Discovery, Northeastern University. In this study, we have investigated the carbamate-based inhibitor AM9928, which exhibited high potency against recombinant human MAGL (hMAGL) with IC 50  (2) suitable physicochemical properties with low lipophilicity (ClogP 3-4); (3) good microsomal (> 20 min) and plasma stability (> 120 min); and (4) potentially prolonged pharmacodynamic effects as determined by 1 H NMR spectroscopy, assessing the time required for MAGL reactivation (residence time) following the covalent interaction between ligand and protein.
Since MAGL is important for lipid metabolism which plays various roles in tumorigenesis, we investigated MAGL's role in TNBC growth and tumor colonization in brain. MAGL is highly expressed in TNBCs, which secrete high levels of inflammatory cytokines and chemokines. We hypothesized that tumor growth in the mammary fat pads and tumor cell infiltration across the BBB is facilitated via inflammatory chemokines/cytokines secreted from TNBC cells, leading to activation of HBMECs and resulting in TNBC spreading and colonization in brain. Here, we have performed studies using several human and mouse cell lines: (1) a TNBC human cell line (MDA-MB-231 cells); (2) human and mouse brain-seeking TNBC cell lines (MDA-MB-BrM2 and GFP-4T1-BrM5, respectively; (3) human brain microvascular endothelial cells (HBMECs); and (4) the spontaneous breast metastasis mouse models (syngeneic). We found that TNBC adhesion to HBMECs and transmigration across HBMECs was inhibited by AM9928. AM9928 inhibited TNBC's secretion of inflammatory cytokines such as IL-6 and IL-8, and the angiogenic factor VEGF-A. Notably, AM9928 inhibited in vivo changes in BBB permeability and decreased TNBC colonization in brain. Taken together, these results demonstrate novel mechanisms by which MAGL mediates its effects on brain metastasis by activation of brain microvascular endothelium and modulating BBB permeability. These studies support the potential of clinical application of MAGL inhibitors as a novel treatment of TNBC tumor growth and TNBC-colonization in the brain.

Results
Assessment of the residence time of AM4301 and AM9928. In vitro binding and unbinding of potent hMAGL carbamate-based inhibitor AM9928, as compared to another MAGL inhibitor AM4301, was monitored by 1 H NMR spectroscopy using downfield 1 H NMR spectral pattern as a probe of conformational changes associated with binding [28][29][30] . The chemical shift perturbations (CSPs) induced by binding in the characteristic downfield NMR pattern are shown in Fig. 1. Addition of equimolar amounts of inhibitor in both cases caused significant upfield chemical shift (from 14.9 to ~ 13.9 ppm) for the resonance peak corresponding to the catalytic His269 and smaller shifts for other downfield resonances. Following the fast formation of hMAGL covalent adducts with AM4301 ( Fig. 1a) and AM9928 (Fig. 1b), slow reactivation of the enzyme due to hydrolytic decomposition of adducts was monitored by real-time NMR spectroscopy. The residence time (lifetime) of ligand on a target is equal to the lifetime of covalent adduct which is a period required for adduct to decrease to 1/e (0.367) of its original concentration. The estimated residence time based on NMR time course data for AM4301 is 1.4 h whereas AM9928 residence time is 46 h. Significantly longer in vitro residence time of AM9928 in comparison with AM4301 could be translated to its prolonged pharmacodynamic effect. Based on these observations we selected AM9928 for our studies.
The toxicity effects and time kinetics of treatment with AM9928. First, the toxicity of AM9928 on TNBC cells were analyzed. TNBC cells include the highly invasive MDA-MB-231 and the brain-seeking vari-

AM998 inhibited adhesion and transmigration of breast cancer cells through human brain microvascular endothelial cells (HBMECs).
Next, in vitro adhesion assay was performed using cocultures of the brain-seeking variant MDA-MB-BrM2 cells and HBMECs in the presence of vehicle control or AM9928. MDA-MB-BrM2 cells adhered to the untreated monolayer by as early as 10 min (Fig. 2b). Pretreatment of HBMECs monolayers with AM9928 (100 ng/mL) blocked the adhesion of the tumor cells at time points of 10 and 30 min (Fig. 2b). We then examined the role of MAGL in transmigration of TNBC cells across HBMECs using an in vitro model where HBMECs were co-cultured with MDA-MB-231 cells or with MDA-MB-BrM2 cells 16 . Tumor cell transmigration across HBMECs was significantly inhibited (about 50%) by AM9928 (Fig. 2c).

MAGL mediated effects on chemokines/cytokines. Cytokines are produced by both immune cells
and cancer cells 31,32 . To characterize the potential activity of MAGL inhibitor AM9928 on the inflammatory responses in breast cancer, we used TNBC cells, the MDA-MB-231 and MDA-MB-BrM2 cells, as compared to the immortalized normal breast epithelial MCF-10A cells.  www.nature.com/scientificreports/ First, we examined the effects of AM9928 as compared to the vehicle control on secretion of cytokines/ chemokines in cells treated with either the vehicle control or with AM9928 (250 nM) for 72 h. The supernatants were collected and analyzed by specific ELISA assays for IL-6, IL-4, INF-alpha2, TGF-alpha, TNF-alpha, and IL-8. We observed significant inhibitory effects of AM9928 on IL-6 and IL-8 ( Fig. 3a, b). Both inflammatory cytokines are important in tumor-associated inflammation 4,[32][33][34][35] .
Second, we analyzed the effects of AM9928 on the angiogenic factor VEGF-A secretion and observed significant inhibitory effects of AM9928 on VEGF-A secretion (Fig. 3c).
Third, to determine if chemokines/cytokines are directly involved in activation of HBMECs, without the contact of tumor cells, we prepared TNBC-derived exosomes untreated or treated with AM9928 and tested their direct effects on HBMECs. MDA-MB-231 and MDA-MB-BrM2 derived exosomes caused activation of HBMECs leading to secretion of elevated levels of the pro-inflammatory IL-8 and VEGF-A. AM9928 treated TNBC-derived exosomes significantly inhibited activation of HBMECs, as observed by the secretion levels of IL-8 and VEGF-A (Fig. 3d, e).
In vivo analysis of tumor growth in mammary fat pads and colonization of TNBCs in brain. For these studies, we employed the spontaneous breast cancer metastasis mouse model (syngeneic) of mammary tumor cells GFP-4T1-BrM5 cells, which "home" to brain and form breast metastasis in brain in mice. The GFP-4T1-BrM5 cells secreted IL-6, IL-8, and VEGF, which were inhibited by AM9928 (Fig. 4a). These results were like those obtained with MDA-MB-BrM2 cells (Fig. 3). In addition, AM9928 had similar effects on GFP-4T1-BrM5 cell survival, adhesion, and transmigration, as those observed with MDA-MB-BrM2 cells (data not shown). Therefore, we employed GFP-4T1-BrM5 cells for the in vivo studies.
To examine the effects of AM9928 on TNBC tumor growth and BBB integrity, GFP-4T1-BrM5 cells were administered (5 × 10 4 cells) into the mammary fat pads of BALB/c mice and were injected with either the vehicle control or with AM9928 as shown in Fig. 4b (n = 10 mice per group per treatment). The GFP-4T1-BrM5 tumor cell growth in mammary fat pads and tumor cell seeding in the brain was observed by in vivo imaging and immunohistochemistry. Tumor growth in mammary fat pads was significantly reduced in mice treated with AM9928 as compared to mice treated with vehicle control (Fig. 4c). Quantitation of GFP-4T1-BrM5 tumor cell colonization in brain was determined by ex vivo imaging of the brain and by immunohistochemistry using GFP antibody. The expression of GFP positive tumor cells was significantly lower in the mice group treated with AM9928, while the presence of GFP-4T1-BrM5 tumor cells in brain was significantly higher in the untreated GFP-4T1-BrM5 cells treated mice or the vehicle control treated mice (Fig. 4d, e). Of note, we did not observe any effects of AM9928 on body weight of mice or toxicity in the treated AM9928 mice, as compared to untreated mice or mice treated with vehicle control (data not shown).
Increased in BBB permeability was found in mice administered with tumor cells or with vehicle treated mice, while mice treated with AM9928 had lower BBB permeability changes as compared to control mice at day 28 (Fig. 5a). We then analyzed BMEC-tight junction proteins' expression of ZO-1 and Claudin-5 in vivo in normal brain, as compared to TJs in tumor brains from syngeneic GFP-4T1-BrM5 mammary tumor cell model. ZO-1 is the main scaffolding protein and Claudin-5 is known as the TJ sealer protein of the BBB. As shown in Fig. 5b, both expression of ZO-1 and Claudin-5 were abundant in the normal brain vasculature. Following GFP-4T1-BrM5 cell administration to the mammary fat pads, the GFP-tagged tumor cells migrated across the BBB after about 4 weeks. The GFP tagged tumor cells were detected by immunostaining with either Monoclonal Pan-Cytokeratin (Pan-CK) antibodies or GFP antibodies. No staining of normal brain sections with Pan-ck antibodies was observed (data not shown). These tumor cells were usually located around the brain capillaries and in-close proximity to BMECs (BMECS were detected with CD31 antibodies) (Fig. 5c).
Both ZO-1 and Claudin-5 structures were damaged in the brain tumor vasculature (Fig. 6a) as compared to the control brain (Fig. 5b). In some images, both ZO-1 and Claudin-5 structures withing BMECs were seen to colocalized with tumor cells, indicating the proximity of BMECs to the tumor cells in vivo. Interestingly, following treatment with AM9928, the expression of both ZO-1 and Claudin-5 was less impaired as compared to mice treated with vehicle control (Fig. 6a). The number of CD31 + vessels expressing TJ proteins, ZO-1 and Claudin-5, was performed and images were analyzed using Adobe Photoshop CS2. The expression of ZO-1, as determined by average sum of intensity was 582,096 and following treatment with AM9928 the average sum of intensity was 610,920 (Fig. 6b). The average sum of intensity of Claudin-5 expression in untreated group was 518,599 and following treatment with AM9928 the average sum of intensity was 681,457 (Fig. 6c). Although these differences in TJ numbers were not statistically significant between the untreated mice and the treated AM9928 mice, both ZO-1 and Claudin-5 were observed as less damaged structures in the AM9928 treated mice, as compared to the vehicle treated mice.
In summary, the number of mice positive with mammary tumors and brain tumors was significantly higher in the vehicle treated group as compared to AM9928 treated mice (Table 1). Importantly, the number of alive mice at day 28 was higher in the AM9928 treated mice as compared to the vehicle control group (Table 1). Thus, AM9928 significantly inhibited changes in the BBB permeability and reduced TNBC colonization in brain.

Discussion
During tumor progression, cells undergo reprogramming of metabolic pathways that regulate glycolysis and the production of lipids 23,[36][37][38] . The energy supply of lipids comes from de novo synthesis rather than circulating lipids during tumor development, which are modulated and regulated by MAGL [36][37][38][39][40] . Since MAGL is important in assigning the lipid stores in the direction of pro-tumorigenic signaling lipids in cancer cells 39,40 , we studied the mechanisms by which MAGL contributes to promoting TNBC metastases.   (e) Quantitative analysis of tumor cells in the brain: Murine tumor cells in brain were detected by GFP immunostaining as described above. The tumor areas at day 28 were detected by immunostaining with GFP antibody: n = 10 mice/treatment; *p < 0.05, Mann-Whitney U-test; **p < 0.005 as compared to vehicle control. www.nature.com/scientificreports/ www.nature.com/scientificreports/ 2-AG on immune cells and TME usually involve CB2 cannabinoid receptors, although CB1 independent effects such as leukotriene biosynthesis and LTB4 activation could be observed 41 . MAGL is important for CB1 receptor (de)sensitization and the endocannabinoid tone. Assessment of MAGL inhibitors for their in vitro potency was performed to select compounds for the in vitro and in vivo studies. The application of protein-based real-time 1 H NMR spectroscopy allowed us to determine the residence time on target for potent MAGL inhibitor AM9928 (~ 46 h) (Fig. 1). The longer inhibitory effect www.nature.com/scientificreports/ of AM9928 strongly suggest prolonged pharmacodynamic effect of AM9928 which is required for our in vivo studies. Although previous studies 42 showed that AM9928 lacked affinity for the cannabinoid receptors CB1 and CB2 receptors, it is important to exclude any effect of AM9928 on CB1 and/or CB2 receptors. Therefore, future studies will test the effects of AM9928 on mice lacking CB1/CB2 receptors.

Scientific Reports
Breast cancer progression and its responses to therapy are regulated by inflammation that promotes or suppresses tumor progression. While chronic inflammation initiates tumor progression and facilitates treatment resistance, acute inflammation stimulates the maturation of dendritic cells and antigen presentation, leading to increased anti-tumor immune responses. Acute and chronic inflammation are regulated by interferons, the interleukins, the chemokine family, mesenchymal growth factors, the tumor necrosis factor family and adipokines [43][44][45] . Key pro-inflammatory cytokines involved in inflammation include interleukin-1 (IL-1), IL-6, tumor necrosis factor (TNFα), IL-8, IL-12, IFN-γ and IL-18 43,[46][47][48][49][50] . Therefore, we analyzed the effects of MAGL on these inflammatory cytokines/chemokines. Based on our results, AM9928 inhibited the secretion of tumor-inflammatory molecules IL-6 and IL-8 and the angiogenic factor VEGF-A in MDA-MB-231 cells and MDA-MB-BrM2 cells (Fig. 3) as well as the murine GFP-4T1-BrM5 cells (Fig. 4a). The tumor niches are also the source of proinflammatory cytokines 4,10 , which are secreted by both autocrine and paracrine manner though activation of STAT3 45 . The strong inhibition of IL-6 by AM9928 indicates the potential of AM9928 to target inflammation associated with TNBC. Therefore, MAGL may regulate lipid quality and/or quantity to promote aggressiveness such as migration and inflammation in breast cancer cells.
VEGF-A/VEGF-R signaling functions as an important survival pathway in breast cancer cells [51][52][53] . VEGF-A promoted tumor cell self-renewal through VEGF-A/VEGF-R/STAT3 signaling resulting in activation of Myc, Sox2 and STAT3 [51][52][53] . High VEGF-A levels strongly correlated with both STAT3 and Myc expression as well as with tumor metastatic potential. Interestingly, we found significant inhibition of VEGF-A expression by AM9928 in human and murine TNBC cells (Figs. 3c, 4a), strongly suggesting the effectiveness of targeting MAGL in inhibition of angiogenesis.
Breast cancer-derived exosomes accumulate in the lung, spleen, and bone marrow of naïve mice. At these sites, the exosomes content rich in IL-6 causes pro-metastatic alterations associated with reduction of T cell proliferation, NK cell cytotoxicity and modulation of immune responses. However, the role of TNBC-derived exosomes on HBMECs is not known. Here we show that HBMECs were activated by TNBC-derived exosomes which resulted in secretion of IL-8 and VEGF-A, thereby facilitating tumor transmigration across HBMEC, and promoting tumor colonization. Further, AM9929 treated TNBC-derived exosomes inhibited HBMECs activation as indicated by the secretion levels of IL-8 and VEGF-A. Thus, TNBC-derived exosomes may act as important mediators of intercellular communication between TNBCs and brain endothelium facilitating the formation of pre-metastatic niches and contributing to tumor dissemination in brain and tumor angiogenesis.
Since MAGL is expressed in TNBCs and in the immune and stromal cells of the tumor microenvironment, inhibition of MAGL in both compartments is essential for inhibition of tumor growth and pathogenesis. Indeed, recent studies showed that MAGL deficiency in the tumor microenvironment slows tumor growth in non-small cell lung cancer 41 . Further, the MAGL inhibitor JZL184 was shown to reduce osteolytic bone metastasis in mouse models of advanced breast and prostate cancer and osteosarcoma, and inhibited skeletal tumor growth, metastasis, and the formation of ectopic bone in models of osteosarcoma 54 . Inhibition of breast cancer MDA-231-BT cell motility in vitro by JZL184 (JZL, 10 μM) and MAGL knockdown prolonged the survival in mice injected with metastatic osteosarcoma cancer cells 54 . These results are similar to those obtained in our model system of TNBC, showing that AM9928 targeted both the tumor cells and the tumor niches and inhibited tumor growth in the mammary fat pads (Fig. 4). Future studies in our lab will examine if AM9928 has similar effects as JZL 184 in inhibition of cancer-related bone damage.
The permeability of the BBB plays a crucial role in brain metastasis and is implicated in the initial stages of cancer cell extravasation. In the presence of invading tumor cells, the local BBB is altered and exhibits heterogeneous permeability [14][15][16] . The changes in permeability of the BBB-BMEC-TJs are a dynamic process dependent on the interaction between TNBCs and BMECs. Our results showed that TNBC transmigration across the BBB resulted in increased BBB permeability (Fig. 5). These effects could be mediated by IL-6 and IL-8 as well as VEGF, which were secreted by tumor cells and were shown to influence the BBB permeability 15,16 . AM9928 mediated its effects on both the tumor cells and the tumor microenvironment including BMECs. Since AM9928 inhibited tumor growth in the mammary fat pads (Fig. 4) and prevented changes in BBB integrity in vivo, resulting in reduced tumor cell colonization in brain, we conclude that MAGL plays a role in TNBC tumor growth and TNBC transmigration across the BBB, in addition to its roles in neurological and neurodegenerative diseases 25,26 . Since metastasis to the brain was reported to be associated with damage to the cranial bone (osteolysis) 1-3 , it is www.nature.com/scientificreports/ important to investigate whether AM9928 may have bone protective effects in mice bearing the GFP-4T1-BrM5 cells. We plan to examine this question in future studies in our lab. Taken together, we suggest that targeting MAGL may provide a new approach to reducing expression of proinflammatory factors as well as inhibiting tumor growth and tumor cell colonization in brain. The breast tumor metastatic brain variants include the MDA-231-BrM2 tagged to GFP as well as the murine mammary tumor cells GFP-4T1-BrM5 which specifically form metastatic brain tumors [14][15][16] and generate multifocal lesions in the cerebrum, the cerebellum, and the brainstem, with features typical of brain metastasis in cancer patients. All cell lines were incubated in 5% CO 2 at 37 °C, as described (16).

Ethics statement. The animal study protocol was approved by The Institutional Animal
MAGL inhibitor AM9928. MAGL inhibitor AM9928 was synthesized and characterized at the Center for Drug Discovery, Northeastern University. This compound was tested with both species of human and rat MAGL to examine the inhibitory effects. Further, this compound was tested against human FAAH and human ABHD6 42,55 . To this end, AM9928 inhibited human MAGL (hMAGL) with an IC 50 value of 8.9 nM. AM9928 covalently inactivates MAGL by carbamylation of the enzyme's catalytic Ser122 nucleophile. The structure and mechanism of action of AM9928 has been published in the PhD thesis of Dr. Nasr (see link http:// hdl. handle. net/ 2047/ D2023 8396).
Nuclear magnetic resonance spectroscopy and data analysis. Target-based 1D 1 H-NMR spectroscopy was employed for real-time monitoring of the unbinding kinetics. NMR experiments were performed at physiological conditions (37 °C, pH7.4) on Bruker AVANCE 700 MHz spectrometer equipped with a 5-mm triple resonance inverse probe. For the efficient detection of exchangeable downfield resonances, a standard 3-9-19 WATERGATE pulse sequence (p3919pgp) with gradients and additional flipback pulse was used. This sequence significantly reduces unwanted attenuation of peak intensities originated from spin diffusion and chemical exchange with water. Samples of highly purified recombinant hMAGL prepared for NMR binding/ unbinding experiments (0.5-0.6 mL) were 0.2-0.3 mM in 95% H 2 O, 5% D 2 O buffer containing 20 mM sodium phosphate, 100 mM NaCl and 5 mM TCEP (tris(2-carboxyethyl)phosphine) at pH7.4. Ligand titration into the target enzyme was performed using 50 mM stock solutions of inhibitors dissolved in DMSO-d6. Chemical shift referencing was done by using internal DSS (sodium 2,2-dimethyl-2-silapentane-5-sulfonate) standard (20 μM). The data were visualized and processed using Bruker software Topspin 3.2. For all NMR spectra an exponential multiplication (broadening factor lb = 30 Hz) was applied. Characteristic CSPs in the downfield region of hMAGL proton NMR spectra were observed because of fast and complete covalent adduct formation when the potent inhibitor is titrated into the protein in a 1:1 ratio. During subsequent hydrolytic ligand unbinding the integral intensity of the merged peak at ~ 14.0 ppm, which is equal to two protons (His269 and His54), gradually decreases to the value of one proton corresponding to the state when the enzyme is fully reactivated. Integration of this peak allows reliable measurements of decreases in adduct concentration over a period. The NMR peak at 15.9 ppm (His103) was chosen as a reference for integration procedure since its position is not affected upon adduct formation. Plots of the natural logarithm of the adduct concentration versus time yielded a linear relationship for examined inhibitors, indicating pseudo first-order kinetic behaviors (data not shown). In this case, the slope of curve represents the enzyme reactivation constant (k r ) which allows evaluation of the half-life (t 1/2 = ln2/k r ) of covalent adducts as well as residence time (τ = 1/k r ) of ligands on the target enzyme.
Isolation of exosomes. The culture supernatants of MDA-MB-231 and MDA-MB-BrM2 cells untreated or treated with AM9928 for 24 h, at approximately 65% confluence and were harvested after 16 h conditioning in serum-free media. Cells and debris were cleared from the supernatants by centrifugation (500 g, 10 min) followed by filtration using 0.22-micron filters (Millipore Inc.). Exosomes were prepared from cell-free supernatants using the Exosome Isolation Kit (Kit# EIK-01, Creative Biolabs, NY). The quantitative and qualitative analysis of exosomes were performed on double sandwich enzyme-linked immunoassay using the Total Exosome Capture & Quantification Kit (Kit#EQK-04Creative Biolabs).
In vivo effects of MAGL on BBB integrity and TNBC colonization in brain. Female (6 weeks) BALB/c mice were purchased from Jackson Laboratories (Bar Harbor, ME). The mice were housed at an AAALAC-accredited facility at Beth Israel Deaconess Medical Center, Boston. The mice were handled in accordance with the animal care policy of Harvard Medical School. Mice were euthanized humanely by CO 2 inhalation in the end of the experiments following treatment and tumor samples were harvested for further study as described below. www.nature.com/scientificreports/ We have selected AM9928 for our in vivo studies based on its prolonged target engagement. We expected that the prolonged inhibitory effect of AM9928 would be translatable to a longer pharmacodynamic effect when compared to other MAGL inhibitors such as AM4301.To that end, we studied the effects of AM9928 on BMEC-TJs and tumor colonization in the brain using the spontaneous breast cancer metastasis mouse model (syngeneic) of mammary tumor cells. We used GFP-4T1-BrM5 cells which migrate to the brain [14][15][16] and form breast metastasis in the brains of Balb/c mice. We administered GFP-4T1-BrM5 tumor cells (5 × 10 4 cells) into the mammary fat pads of Balb/c mice. More than 70% of these mice developed mammary tumors within 3 weeks, while brain microtumors were developed in about 5 weeks. Here, following administration of GFP-4T1-BrM5 cells, mice were injected with AM9928 (10 mg/kg, i.v.) or the vehicle control twice a week for 3 weeks (10 mice/ group/treatment). Tumor sizes in the mammary fat pads were measured using calipers, and the volume was calculated, using the formula: V = 0.52 × (length) × (width) 2 . The BBB integrity at the end of the experiments was analyzed by Evan blue test in control groups and in mice treated with AM9928 as compared to vehicle control. Briefly, Evans's blue (EB) (Sigma Chemical Co. St. Louis, MO. USA.) dye (25% in 0.9% NaCl solution) was intravenously injected at dose of 25 mg/kg under anesthesia. One hour after the injection, animals were sacrificed. Brains were weighed, clipped, and individually placed within formamide p.a. (2 mL/brain). The content of dye extracted from each brain was determined by spectrophotometer (Photometer 4010, Boehringer) at 620 nm and compared to standard graph created through the recording of optical densities from serial dilutions of EB in 0.9% NaCl solution. For in vivo imaging, spectral fluorescence images were obtained using a Maestro-based imaging system (CRI Inc., Woburn, MA, USA). Five sets of side-by-side whole-brain images of animals with tumors from the GFP-4T1-BrM5 cells treated with vehicle control or with AM9928 were obtained. In the end of the experiment, mice were sacrificed, and brain and mammary fat pad tissues were collected for further analysis.

Data availability
All data generated or analyzed during this study are included in this published article.  16-19356 (2016).