Synthesis of a biomimetic zwitterionic pentapolymer to fabricate high-performance PVDF membranes for efficient separation of oil-in-water nano-emulsions

Oily wastewater from industries has an adverse impact on the environment, human and aquatic life. Poly(vinylidene fluoride) (PVDF) membrane modified with a zwitterionic/hydrophobic pentapolymer (PP) with controlled pore size has been utilized to separate oil from water from their nano-emulsions. The PP has been synthesized in 91% yield via pentapolymerization of four different diallylamine salts [(CH2=CHCH2)2NH+(CH2)x A−], bearing CO2−, PO3H−, SO3−, (CH2)12NH2 pendants, and SO2 in a respective mol ratio of 25:36:25:14:100. Incorporating PP into PVDF has shown a substantially reduced membrane hydrophobicity; the contact angle decreased from 92.5° to 47.4°. The PP-PVDF membranes have demonstrated an excellent capability to deal with the high concentrations of nano-emulsions with a separation efficiency of greater than 97.5%. The flux recovery ratio (FRR) of PP-5 incorporated PVDF membrane was about 82%, which was substantially higher than the pristine PVDF.

Oily wastewater is one of the potential contributors to environmental pollution, and it has become a significant concern owing to its adverse impact on the ecosystem. The major contributor to oil pollution is the industries that are not limited to petrochemicals, electroplating, mining, and gas/oil production units 1 . The high demand for oil needs the oil's rapid offshore movement, and nature has witnessed several deadly oil spills. For instance, the Deepwater Horizon oil spill in the Gulf of Mexico has triggered the alarm of the difficulty of the oil-water separation 2 . Owing to its high significance, the development of advanced technologies and methods for the reclamation of the water from the oil-contaminated water has become an area of deep concern. The conventional methods such as adsorption, air floatation, centrifugation, and gravity settling might require high energy and are inefficient in separating oil from water 3 .
To separate oily emulsions, the membranes are considered effective in separating the oil from the waterbased on the size-sieving. More control on the pore size of the membranes is required in the separation of the nano-emulsions as they consist of nano-sized oil droplets which easily pass through the rough membranes. Several super-selective materials and surfaces have been reported for the oil/water separation. These materials have been used in the forms of meshes 4 , cotton 5 , foams 6 , sponges, woven/non-woven fabrics 7 . Most of these designed materials effectively separate the floating oils but may face challenges separating the emulsions. Based on the dispersed phase diameter, the different nature of the oil/water mixture can be defined. If the diameter is more than 150 μm, it is termed as free oil and water mixture, while a term of dispersion is used when it is in the range 20-150 μm. The emulsions are generally defined when the diameter is < 20 μm 2 . Due to the complex nature of the emulsions, conventional techniques such as skimming, and gravity separators are ineffective for separating the emulsions. Chemical emulsion breaking may be effective, but high operation costs, significant Materials. 2,2′-Azobisisobutyronitrile (AIBN) (≥ 98%) was purchased from Fluka Chemie AG and crystallized from chloroform-ethanol. Dimethylsulfoxide (DMSO) (≥ 99.5%), N,N-dimethylacetamide (DMA), Bovine Serum Albumin (BSA), and surfactant TWEEN ® 80 were purchased from Sigma-Aldrich. All water used was of Milli-Q quality. A Spectra/Por (Spectrum Lab., Inc.) membrane (MWCO 6000-8000) was used for dialyses. Monomers 1 33 , 2 34,35 , 3 36 , and 4 37 were synthesized. The Alfa Aesar-44080 poly(vinylidene fluoride) was purchased with the molecular weight of 350 KDa 38 .
Synthesis of PP 5. To a solution 1 (1.83 g, 10 mmol), 2 (3.28 g, 14.4 mmol), 3 (2.19 g, 10 mmol), and 4 (1.98 g, 5.6 mmol) in DMSO (11 g) in an RB flask was absorbed SO 2 (2.56 g, 40 mmol). Initiator AIBN (180 mg) was added to the solution and stirred at 65 °C for 24 h. The thickened mixture (turbid in water) was dialyzed against distilled water for 36 h, during which the polymer solution became turbid and separated as a thick gel at the bottom of the dialysis tube. The whole mixture was freeze-dried to obtain 5 as a white solid (10.3 g, 91%). Note that a HCl unit is depleted from monomer 2 during dialysis to give the zwitterionic motifs in the repeating units of PP 5. It is calculated that 283.0 mg of PP 5 contains repeating units 0. 25

Preparation of oil in water emulsion.
Oil-in-water emulsions were prepared by adding diesel (1 g) into water (1 L) in the presence of surfactant Tween-80. The mixture was kept under vigorous stirring at 600 rpm at room temperature for 12 h. It was furthermore sonicated for 2 h. The average size of the oil-in-water emulsions was found about 92.71 nm with the help of the Malvern Zetasizer (Fig. S1). The oil/water separation, water flux, and the antifouling performances of the pristine and PP 5-PVDF membranes were accomplished by fitting the membranes into the dead-end filtration unit. The nitrogen cylinder was used to adjust and supply the appropriate trans-membrane pressure. The membranes were pre-compacted by pure water at a pressure of 8 bar for 1 h. The oil residue in the permeate was determined by the UV-Vis spectrophotometer.

Results and discussion
Synthesis and characterization of PP 5. We set out to synthesize a new zwitterionic pentapolymer (PP) 5 ( Fig. 1) containing a variety of chelating motifs CO 2 − , PO 3 H − , SO 3 − and NH 2 using cyclopolymerization protocol to pursue our planned modification of PVDF membrane for separation of the nano-emulsions. The work is inspired by the zwitterionic phosphatidylcholine headgroups, which contain cationic and anionic groups and are present in the cell membrane phospholipid bilayer. Due to this zwitterionic behavior of the cell membrane, it has fouling-free behavior. Naturally occurring zwitterions, very common in cell membranes, proteins, etc., motivated us to synthesize PP 5 which has pH-responsive zwitterionic motifs conferring charge neutrality, high hydrophilicity, strong dipole pairs, etc. A tightly and stably bounded water layer near zwitterionic polymers via strong electrostatically induced hydration, imparts the antifouling property of zwitterionic polymers by increasing the energy barrier for the adsorption of foulants 39 .
AIBN-initiated cyclopolymerization of monomers 1, 2, 3, 4, and SO 2 afforded PP 5 in an excellent yield of 91% (Fig. 1). Since the reactivity ratios of SO 2 and the diallyl monomers are almost zero, the PP will have the monomers in random distribution alternated by SO 2 . At such a high conversion, the feed ratio is expected to match the monomer incorporation ratio. As such, the ratio of monomers 1, 2, 3, and 4 incorporated in the polymer was taken as 25:36:25:14, respectively. Note that an HCl molecule was depleted from the monomer 2 repeating unit during dialysis to give the zwitterionic phosphonate motifs in the polymer.
Treatment PP 5 with NaOH is expected to generate anionic polyelectrolyte 6, which has numerous ligands involving N and O, whereby their Log[basicity constants], i.e., the pK a s of their conjugate bases, range from − 2.1 to 10.5 36,40,41 (Fig. 1). The amino-carboxylate, -phosphonate, and -sulfonate motifs provide several chelation centers. The pH-responsiveness of the polymer thus provides a greater latitude to trap various metal ions.
The 1 H and 13 C NMR spectra of PP 5 and monomer 1 is shown in Figs. S2 and 2, respectively. The absence of alkene protons in the range 5-6 ppm and alkene carbons at 127-128 ppm confirms the absence of any residual www.nature.com/scientificreports/ double bond in the polymer, which suggests degradation chain transfer as the termination process involving allylic hydrogen of the monomers 42 (Fig. 2). The carboxylate signals at δ182 ppm and 31 P signals at δ5.72 ppm confirm the incorporation of monomers 1 and 2 in 5. The elemental analysis supports the incorporation of SO 2 and SO 3 -. IR bands 1296 and 1125 cm −1 were attributed to the SO 2 groups in PP 5 43 . The absorptions at 1413 and 1648 cm −1 were assigned to the stretching vibrations 44 of COO -. A weak band at 1717 cm −1 could be attributed to the CO 2 H owing to the equilibration: -NH + (CH 2 ) 3 CO 2 − ⇋ -N(CH 2 ) 3 CO 2 H. The band at 747 cm −1 is assigned to methylene chain (CH 2 ) 12 . The IR spectrum indicates the presence of the sulfonate group by its characteristic bands at 1180 and 1033 cm −143 . The band at 1205 cm −1 can be assigned to the stretching frequency of P=O 34 .
It was difficult to obtain molar mass of PP 5 using GPC, since the presence of CO 2 − , PO 3 H − , SO 3 − and NH + motifs force the polymer to stick to the column materials. A similar observation was found earlier 45 . TGA analysis of PP 5. The TGA curve of PP 5 shows a weight loss of 6% up to 200 °C owing to the removal of moisture (Fig. S3). A sharp loss of 23% in the range 220-275 °C is attributed to the decomposition releasing SO 2 ; the polymer is calculated to have 22.6 wt% SO 2 . Another major loss of 27% in the range 275-470 °C is accounted for the loss of some of the pendants. The residual mass of 36% remaining at 800 °C could be attributed to some nitrogenous and phosphorous derivatives. The polymer thus remains very stable up to 220 °C. Solubility behavior. The water-insolubility of PP 5 is attributed to the effects of interactions of the zwitterionic motifs, which force the polymer backbone to adapt globular conformation 30,46 . The polymer was found to be insoluble in 1 M HCl and 1-5 M NaCl, but soluble in the presence of KBr. CSC value of KBr was determined to be 3.44 M. The stronger binding ability of Br -(as compared to Cl − ) to the NH + centers can disrupt the zwitterionic interactions and thereby leading to extended conformation and thus promoting water-solubility.
A sample of PP 5 (120 mg) was found to be water-soluble in water (12 mL) in the presence of NaOH (15 mg, 0.375 mmol). The sample (120 mg) is calculated to have repeating units 0.106 mmol 1, 0.153 mmol of 2 (-HCl), 0.106 mmol of 3, 0.0594 mmol of 4 (vide supra). Therefore, upon the NaOH treatment, the pH-responsive equilibrium: 5 ⇋ 6 would reside on the right side, whereby the anionic motifs lead to extended conformation and water-solubility. www.nature.com/scientificreports/ Viscosity data. The viscosity plot of PP 5 in the presence of NaOH in 1 M NaCl is shown in Fig. S4. The intrinsic viscosity was found to be 0.0549 g dL −1 The low viscosity may be attributed to intramolecular hydrophobic association involving (CH 2 ) 12 NH 2 pendants, which helps to coil up the polymer backbone thereby reducing hydrodynamic volume.
Characterization of PVDF membranes. FTIR and Thermal analysis. The pristine PVDF and PP 5 zwitterionic polymer doped PVDF were qualitatively investigated with the help of FTIR spectroscopy. The band that appeared at 825 cm −1 was assigned to the C-F stretching vibration of PVDF. The PVDF backbone C-C-C asymmetrical stretching vibrations were observed at 877 cm −1 . A prominent absorption band appeared at 1175 cm −1 was assigned to the -CF 2 symmetrical stretching of PVDF 47 . The fluoro compound generally exhibited strong absorption in the range of 1000 cm −1 to 1400 cm −1 . The characteristics of PVDF β-phase absorption bands observed at 604 cm −1 and 1249 cm −1 are the fingerprint of the β-phase of the PVDF 48 . The various absorption bands that appeared at 501 cm −1 , 1077 cm −1 , 1160 cm −1 , and 1388 cm −1 are the characteristic peaks of the absorption band of the α crystalline phase of PVDF 49 . The PVDF is a fluoro compound, and it has shown strong absorbance in that region, which was evident from the FTIR of the pristine PVDF (Fig. 3A). Due to the -C-H's symmetric stretching, the absorption band was observed at 2887 cm −1 , whereas the asymmetric stretching was assigned to the absorption band at 2977 cm −149 . In the case of the PP 5-containing membranes such as M-1 to M-3, all the characteristic peaks of the PVDF membranes have been observed (Fig. 3). Most of the polymer's absorption bands have appeared very close to the absorption band of the PP 5 and merged with the absorption band of the PVDF. However, the region in which the amino group has been absorbed remained the critical factor in recognizing the successful incorporation of the PP 5 into the PVDF. For instance, in the case of M-1, the two partially separated absorption bands appeared at 3246 cm −1 , and 3340 cm −1 , and these bands appeared owing to the primary amines present in PP 5. However, these bands were entirely absent in the pristine PVDF (Fig. 3A). The thermal stability of the various PVDF membranes was evaluated with the help of thermogravimetric analysis (TGA) in the temperature range 20-800 °C, whereas the temperature was changed 10 °C/min. The pristine or controlled PVDF membranes have shown a sharp loss in weight at 430 °C. The PP 5-PVDF membranes have shown the decomposition temperature in the range 450-456 °C (Fig. S5). TGA has demonstrated that incorporating PP 5 into the PVDF membranes does not compromise their thermal stability. It is according to the literature that fluoropolymers are more thermally stable compared to polymers consisting of hydrocarbons. The high strength of the PVDF is attributed to the C-F bond's high dissociation energy 50 .
Morphological and elemental analysis of PVDF membranes. The surface morphologies of the membranes produced from the 20% PVDF in DMA were scanned with the help of the field emission-scanning electron microscope. Figure 4  Fabricated pristine and mixed matric PVDF membranes were furthermore evaluated to understand the morphology of the skin layer and the base of the PVDF membranes. The best efforts have been made to preserve the morphology while tearing the membranes for the cross-sectional view. The cross-sectional morphology was preserved by dipping the different membranes into the liquid nitrogen, thereby making the membrane brittle www.nature.com/scientificreports/ and easily breaking into two pieces by applying a small force. In the cross-sectional view, irrespective of the membranes types, it has been observed that the membranes exhibited an asymmetric structure. All membranes have consisted of the top skin layer, and finger-like projections started from the immediate base of the skin layer (Fig. 5). A typical spongy base was observed in the membranes; however, the length of the dense spongy base varied from membrane to membrane. It is interesting to discuss how the difference in spongy and finger-like structure has been produced in the various membranes after introducing the zwitterionic polymer PP 5. The high mutual diffusivity of the polymer containing DMA and the water resulted in the formation of the asymmetric structure, and this fact is well known 51 . In the case of the pristine PVDF, the short finger-like projections have been observed, and the rest of the part consisted of the spongy structure. In the case of the mixed matrix membrane, a substantial change in the cross-section of the membranes has been observed. The finger-like projections were grown in breadth and continue in length towards the bottom of the membrane as the amount of the PP 5 was increased in the membranes from M-0 to M-3. The dense skin layer was immediately formed as the polymer dope cast solution immersed into the coagulation bath owing to the rapid out-diffusion of the solvent resulting in the instant solidification of the external membrane surface. After that, the nonsolvent inward diffusion enhanced, which caused the membrane coagulation and generation of the finger-like projection, which initiated immediately after the skin layer and projected downwards to the base. Interestingly, this effect was more pronounced in the mixed matrix PVDF membranes than the pristine PVDF membrane. This behavior in the mixed matrix PVDF membranes can be explained due to the presence of the hydrophilic groups 52 . The water was more attracted inward during the phase inversion process due to the presence of hydrophilic PP 5, resulting in the enhanced rate of mass transfer between water (nonsolvent) and solvent. The fast mass transfer was due to the presence of sulfonate, phosphonate, carboxylate, and quaternary ammonium motifs, resulting in longer finger-like projections. From the cross-section, it is clear that a spongy structure is present in all of the PVDF membranes. The spongy structure was formed as the polymer solidified, which slowed down the mass transfer between the water and solvent. The spongy structure was more prominent and covered almost more than half of the cross-section in the case of the pristine PVDF membrane. The hydrophobic nature of the PVDF slows down the process, which caused the more dense coverage with the spongy structure. The spongy structure decreased as the hydrophilic PP 5 increased in the PVDF. In the case of M-2 www.nature.com/scientificreports/ and M-3, the spongy structure substantially decreased, the presence of PP 5 facilitated and kept the sufficient movement of the water and solvent during the solidification of the polymer. This has caused the reduction in the spongy component in the cross-section of the PP 5-PVDF membranes. It has been shown that incorporating the various concentration of the PP 5 has a significant impact on the subsurface geometry of the membranes. As we discussed, PVDF is a well-known hydrophobic material. Sometimes, it is a disadvantage to using it for wastewater treatment as it may show resistance in the permeation of the water. Usually, hydrophilic moieties are used to improve the hydrophilicity of the PVDF membranes 53 . However, the interaction of hydrophilic moieties with the PVDF membranes is poor due to the substantial difference in their surface energy. The PP 5 zwitterionic polymer is more compatible with the PVDF membrane due to the presence of (CH 2 ) 12 NH 2 pendants www.nature.com/scientificreports/ in PP 5, which is hydrophobic and provides a better opportunity for its interaction with the PVDF membrane. The elemental mapping has provided critical information about the presence and spread of the PP 5 PVDF membranes. The spread of S, N, O, and P was present throughout the membrane, indicating that the PP 5 spread throughout PVDF (Fig. 6).
Hydrophilicity of PVDF membranes. Pristine PVDF membranes are hydrophobic, which affects their performance during wastewater treatment. Owing to its less attraction towards the water, the optimum performance of the membrane has remained a critical challenge during the separation of the oil emulsified water. The separation performance of the membranes critically depends upon the surface wettability and the appropriate pore size. Surface wettability is one of the significant factors contributing to the efficient separation of the emulsified oil from water. The water contact angle on the surface of the pristine PVDF membrane was 92.5° that has shown that the surface is hydrophobic and not water friendly. This finding is according to the literature 54 .
A gradual decrease in contact angle has been observed as the concentration of the PP 5 increased in the PVDF membranes. The contact angle reached 47.4° from 92.5° when the concentration reached up to 0.5% in PVDF (Fig. 7). From the PP 5 structure, it has been clear that it contained a range of anionic and cationic groups such as carboxylate (CO 2 − ), phosphonate (PO 3 H − ), sulfonate (SO 3 − ), and quaternary ammonium motifs. The specifically designed water-loving polymer caused a drastic decrease in water contact angle, and it dropped to 47.4°. Overall, the PP 5 backbone is zwitterionic, and both positively charged and negatively charged groups participated in improving the hydrophilicity of the PVDF membranes, which made them highly effective for the separations of the nano-emulsions (vide infra).

Separation of oil-in-water nano-emulsions by PP-5-PVDF membranes. Oil and water separation
has become critical owing to the rising exploration and industrial applications. Separation of the floating oil is easy, and it can be accomplished by fabricating the super-selective wettable surfaces, which generally have a big- www.nature.com/scientificreports/ ger pore size. The separation phenomena mainly depend upon the selectivity of the surface where surfaces are sharp enough to recognize the non-polar component from water and water from the non-polar oil. However, these sorts of materials sometimes may remain effective for separating the emulsion but, under pressure, might fail owing to the lack of screening potential of the emulsified oil from water. The separation performance has become more complicated when the stabilized oil droplet in emulsions is in the nano range. In the case of nanoemulsion, more control on surface chemistry and pore size is required to separate the nano dispersed emulsified oil drops. The transient fluxes and rejections were recorded at a different transmembrane pressure of 4, 5, and 6 bar. The separation efficiencies of the various PVDF membranes were calculated by Eq. (1) 55 : where %Eff is the percentage of separation efficiency, C o is the oil content in the feed, and C p is the oil content in the permeate.
The pristine PVDF has shown the lowest rejection at all the evaluated pressure of 4-6 bar. The rejection of the pristine PVDF membrane was observed in the range of 90-92%. The introduction of the PP 5 into the PVDF membrane has shown a noticeably positive impact, and the membrane performance was improved substantially. All the PP 5 incorporated PVDF membranes exhibited high performance compared to the pristine PVDF membranes. For instance, the rejection for M-1, M-2, and M-3 membranes was observed in the range 97-97.8%, 96.4-97.7%, and 95.7-96.4%, respectively. The highest rejection has been observed with the PP 5-PVDF named M-2 and M-3, which contained 0.1% and 0.25% PP 5, respectively (Fig. 8).
A significant impact on the permeation flux of the PVDF membranes has been observed after incorporating the PP 5. The permeate flux was calculated by Eq. (2) 56 : where J is the permeate flux, V is the permeate volume in L, A is the effective area of the membrane (m 2 ) and t is the time of the permeation (h).
At 4 bar pressure, the incorporation of 0.1% PP 5 has shown an increase of 73% of flux compared to the pristine PVDF. The flux increased more than 300% when the doping of PP 5 reached from 0 to 0.5% in PVDF. The permeate flux of the PVDF membrane was in the order of PP 0.5% -5-PVDF › PP 0.25% -5-PVDF › PP-0.1% -5-PVDF › pristine PVDF (i.e., M-3 › M-2 › M-1 › M-0) (Fig. 9). The oil rejection of the membranes was in the order M-1 › M-2 › M-3 › M-0. The permeation flux and rejection are trading off; as the flux increases, the rejection was slightly compromised in the PP 5-PVDF membranes. As the M-3-PVDF membrane (PP 0.5% -5-PVDF) has shown a substantially high flux, its rejection is compromised somewhat compared to M-1 and M-2. www.nature.com/scientificreports/ The antifouling behavior of the M-2 membrane has been further evaluated owing to its appropriate flux and better oil rejection. The membranes were compacted at a pressure of 8 Bar for 1 h, and an antifouling study was performed at a transmembrane pressure of 4 bar. The pristine PVDF (M-0) and PP 5 incorporated PVDF (M-2) were exposed to the 1000 ppm oil-in-water emulsion, and after every 15 min, the flux was recorded. After this, the pristine PVDF (M-0) and PP 5 incorporated PVDF (M-2) were exposed to Bovine Serum Albumin (BSA) solution. For 30 min, it was kept with BSA, and after that, every 15 min, the permeate flux was recorded. The pristine PVDF and PP 5 incorporated PVDF membranes were washed with deionized water to wash out the adsorbed BSA. The flux recovery of the washed membranes was analyzed by exposing them to the emulsions at the same pressure of 4 bar. The Flux Recovery Ratio (FRR) of the pristine PVDF membranes was found to be about 60%, whereas the FRR of the PP 5 incorporated PVDF membrane was about 82% (Fig. 10). The membrane was better equipped to fight against fouling when it was combined with the PP 5. The results have shown that the pristine PVDF membrane was tough to recover.
Mechanism of oil-in-water emulsion separation and separation efficiencies. PP 5-PVDF membranes have shown significant efficiencies for the separation of nano-emulsions. The modified PVDF has demonstrated a considerable capacity to deal with the contaminated water with a high concentration of oil emulsions such as 1000 ppm. The separation of the oil emulsions depends upon several factors, such as membrane pore size, emulsions size, surface chemistry of the membrane. The pore size of the membranes is in the range of nm, so these membranes are quite fit for the separation of the small-sized nano-emulsions. It is evident through their SEM images that the pores sizes are small enough to deal with the emulsions. However, some of the refined oil emulsions passed through the pristine PVDF due to its oil-loving nature, which cannot resist significantly the tiny oil droplets and has shown rejection in the range of the 90-92%. Furthermore, the oil formed a cake layer that resulted in the blockage of the pristine PVDF membrane's pores, resulting in the substantial reduction of the flux during operation. The blockage of the pores or the cake layer was severe in the pristine PVDF membrane that it could not be washed simply by deionized water.
The PP 5-PVDF membrane was found effective and more efficient for the separation of the oil-in-water emulsions. The pore size of the membrane was refined, which screened the emulsified oil droplet with great  www.nature.com/scientificreports/ efficiency. Furthermore, it has contained a range of hydrophilic functionalities imparted by the doping of the PP 5. As discussed, the synthesized new PP 5 contained a zwitterionic backbone containing various polar groups such as CO 2 − , PO 3 H − , SO 3 − and NH 2 . These polar groups imparted the hydrophilicity to the PVDF surface and enhanced the potential to attract the water molecules from the emulsions. The presence of PP 5 assisted in forming the hydration layer, which provided the resistance to the PVDF membrane from fouling from the oil. The zwitterionic polymers are being considered as promising next-generation antifouling materials. The zwitterionic polymers used electrostatic interactions to form the hydration shell. It is a strong interaction between the adsorbed water and zwitterionic polymers, resulting in better antifouling characteristics than the materials where interaction consists of hydrogen bonding 57 . Due to the abovementioned reasons, the water passed easily, oil emulsions broke down, and oil was released and prevented from passing through the PP 5-PVDF membranes (Fig. 11). The observed permeate appeared as clear water without any emulsion.
The separation efficiencies of some recent membranes used for separation of oil/water emulsions are compared with that of the current membrane in Table 2, which reveals its notable efficacy.

Conclusions
In conclusion, the de-emulsification of oily wastewater has become critically important to make the water reusable and prevent the collapse of our sustainable ecosystem. Membranes are effective for separating the emulsions, but the separation of the nano-emulsions is a challenging job. In this work, we have synthesized a new zwitterionic polymer PP 5 via cyclopolymerization of diallylammonium salts. The resulting PP 5 contains alternately placed SO 2 and randomly placed three different zwitterionic motifs of carboxylate, phosphonate, and sulfonate groups. The PP 5 was thoroughly characterized by 1 H, 13 C and 31 P NMR spectra. The PP 5 was incorporated into PVDF membranes to improve their performance for the separations of the nano-emulsions. In PP 5-PVDF, the finger-like projections have been observed, and the skin layer has shown well-controlled pores in the nanometer range that can efficiently screen the water; and with the help of PP 5, successfully rejected the nano-sized oil droplets. The TGA analysis has shown that incorporation of the PP 5 did not affect the PVDF membranes' thermal stability, and all the membranes exhibited stability at 400 °C. The presence of polyzwitterionic polymer PP 5 helps in reducing the hydrophobic nature of the membranes and might be responsible for the formation of the  www.nature.com/scientificreports/ hydration layer that assisted in improving the membrane flux and played its role in breaking the oil emulsions. The PP 5-PVDF membrane was exposed to the feed of 1000 ppm of oil-in-water nano-emulsions and permeate collected that contained just 23 ppm of oil. The PP 5-PVDF membrane exposed to the BSA and the FRR was found 82%. This study has shown that the zwitterionic polymers have great potential as membrane modifiers. Their presence can substantially improve the performance of the PVDF membranes for the separation of the oil-in-water emulsions.