Dark adaptation refers to a systematic recovery of visual sensitivity in the dark following exposure to bright lights1. During dark adaptation, multiple processes simultaneously occur to maintain retinal homeostasis, including photoreceptor repolarization2, photopigment regeneration2, blood flow modulation3, a redistribution of photoreceptor signaling proteins4 and interphotoreceptor matrix proteins5, and a reversal of metabolic energy flow6. Visual perception in night vision is rod-mediated; thus, delayed dark adaptation due to impaired rod photoreceptor homeostasis is often the earliest symptom of various retinal diseases, such as age-related macular degeneration (AMD)7, diabetic retinopathy (DR)8, and retinitis pigmentosa (RP)9, the leading causes of irreversible vision loss. Since early detection is essential to prevent further vision loss10,11,12,13, the measurement of dark adaptation kinetics has been proposed as a potential solution for screening early-stage retinal diseases.

Conventional dark adaptation measurement is psychophysical, i.e., dark adaptometry or electrophysiological, i.e., electroretinogram (ERG)7,14. While providing quantitative information on retinal dysfunction, these methods have a limited spatial resolution. Considering the coherent interactions between retinal cells, it appears important to quantitatively examine functional activity simultaneously as well as anatomical arrangement.

Recent developments of intrinsic signal optoretinography (ORG) promise a new methodology for the noninvasive objective assessment of dark adaptation. ORG generally refers to functional optical imaging of the retina. Functional optical coherence tomography (OCT) promises a depth-resolved imaging modality to enable intrinsic signal ORG of the retina15,16,17,18,19,20,21,22,23,24,25,26. Functional OCT has revealed intrinsic optical signal (IOS) distortions correlated with pathophysiological conditions in a mouse model of RP27 and human RP subjects20. Previous OCT studies of the dark-adapted mouse retina revealed outer retinal shortening along with a reduction in the width of a hypo-reflective band between the photoreceptor outer segments (OS) and retinal pigment epithelium (RPE)28,29,30. Lu et al.31 also observed photoreceptor OS elongation following strong light exposure and subsequent recovery, i.e., photoreceptor OS shortening, during dark adaptation in human subjects. However, dynamic monitoring of transient structural and reflectivity changes, which promises a biomarker for rapid assessment of functional integrity of dark adaptation in the retina, remains to be explored.

Here we report intrinsic signal ORG assessment of dark adaptation kinetics in the mouse retina in vivo. The light-adapted retina was initially measured as a baseline, followed by subsequent recording under dark conditions. Dark-induced structural and reflectivity changes in the outer retina were clearly observed in OCT imaging. High-speed monitoring further identified a strong correlation between structural and physiological changes in the outer retina. This study demonstrates the potential of intrinsic signal ORG assessment of dark adaptation kinetics in the living retina.


Imaging features of light- and dark-adapted mouse retinas

Adult C57BL/6 J mice were used in this study. The experiment was performed during the early afternoon when the mouse retina was light-adapted by constant ambient light conditions. Total seven retinal OCT volumes were sequentially acquired at the same region on the dorsal quadrant per mouse; 1st OCT for light-adapted baseline measurement and 2nd–7th OCTs for dark adaptation measurement, acquired at every 5 min interval for 30 min. To first validate the adaptation effects on the retina, a direct comparison between the light-adapted (1st OCT) and dark-adapted (7th OCT) retinas was performed. Figure 1A shows representative OCT images from different mouse retinas. We noted three distinct features in the retina under different light conditions (Fig. 1B). First, it was readily recognizable that dark adaptation caused a reduction in outer retinal thickness. Second, in the light-adapted retina, the interdigitation zone (IZ) and OS tip band of the outer retina (the 3rd outer retinal band) was distinguished, which was absent in the dark-adapted retina, and a hypo-reflective band between the IZ and RPE band was only observed in the light-adapted retina. Third, OCT intensity in the inner segment ellipsoid (ISe) and IS/OS band (the 2nd outer retinal band) markedly decreased in the dark-adapted retina. These results demonstrate that dark adaptation induces transient structural and reflectivity changes in the retina.

Figure 1
figure 1

Comparison of light- and dark-adapted retinas. (A) OCT images of light- and dark-adapted retinas from 3 different mice (A1–A3). (B) Enlarged view of the white rectangle regions in (A1). Red arrows indicate OCT hyper-reflective bands in the outer retina. NFL nerve fiber layer, IPL inner plexiform layer, INL inner nuclear layer, OPL outer plexiform layer, ONL outer nuclear layer, ELM external limiting membrane, ISe inner segment ellipsoid, IZ interdigitation zone, RPE retinal pigment epithelium, CH choroid, IS inner segment, OS outer segment. Scale bars: 100 µm.

Quantitative analysis of retinal changes during dark adaptation

Figure 2A shows repeated measures of OCT during 30 min dark adaptation. To quantify retinal changes, average OCT A-lines were prepared and used for retinal thickness comparison at different time points. We found that the inner retina showed little change (Fig. 2B), while the outer retina revealed a significant reduction in thickness during dark adaptation (Fig. 2C). A repeated-measures analysis of variance (ANOVA) determined that mean outer retinal thickness differed significantly between different time points (p < 0.001). Post-hoc tests using the Bonferroni correction revealed that even 5 min dark adaptation resulted in a significant reduction in outer retinal thickness (Light: 106.8 ± 2 μm; 5 min dark: 105.1 ± 1.9 μm; p < 0.001). However, dark adaptation had not significantly altered inner retinal thickness (Light: 79.42 ± 1.7 μm; 30 min dark: 78.65 ± 2 μm; p = 0.15). Next, the outer retina was segmented into two regions and analyzed (Fig. 2D,E). A slight decline was observed in the outer nuclear layer (ONL) thickness (Fig. 2D). In contrast, the external limiting membrane (ELM)-RPE complex revealed a rapid decline in thickness right after the light went off. A repeated-measures ANOVA determined that mean ONL (p < 0.001) and ELM-RPE (p < 0.001) thickness differed significantly between the time points, and post-hoc tests using the Bonferroni correction revealed that statistically significant changes were first observed after 20 min dark adaptation in the ONL (Light: 58.08 ± 1.6 μm; 20 min dark: 56.76 ± 1.5 μm; p < 0.01) and 5 min dark adaptation in the ELM-RPE complex (Light: 48.73 ± 1.6 μm; 5 min dark: 47.08 ± 1.4 μm; p < 0.001). These results are in line with previous observations29,30 and demonstrate that ELM-RPE complex predominantly affects retinal thinning, accounting for ~ 70% reduction of the outer retina (ELM-RPE displacement after 30 min dark: − 4.07 μm; total outer retinal displacement after 30 min dark: − 5.83 μm).

Figure 2
figure 2

Quantitative measurement of retinal thickness changes during dark adaptation. (A) A sequence of OCT images was obtained every 5 min up to 30 min during dark adaptation. Arrows to indicate outer retinal bands: 1st ELM band (white), 2nd ISe and IS/OS band (yellow), 3rd IZ and OS tip band (blue), 4th RPE band (red). ONL outer nuclear layer, ELM external limiting membrane, RPE retinal pigment epithelium, ISe inner segment ellipsoid, IS inner segment, OS outer segment. Thickness measurement of (B) the inner retina, (C) outer retina, (D) ONL, and (E) ELM-RPE complex during dark adaptation. Data are represented as mean with 95% confidence intervals (error bars). Significant differences between time points were tested using a one-way repeated measures ANOVA with Bonferroni’s post-hoc tests. N = 14. *p < 0.05, **p < 0.01, ***p < 0.001 compared to the light-adapted retina. Scale bars: 100 µm.

The OCT signal is highly sensitive to changes of intrinsic optical properties in excitable cells, such as light scattering, polarization, and absorption fluctuations32,33. As the OCT intensity reduction was apparent in the ISe band (Fig. 1B), we further analyzed relative intensity changes in different hyper-reflective bands, including inner plexiform layer (IPL), outer plexiform layer (OPL), ISe, and RPE. The average OCT A-lines were used for the relative intensity measurement after intensity normalization based on the ONL reflectance. Figure 3 showed that the ISe intensity had significantly reduced after 5 min dark adaptation (p < 0.001). Relative to the baseline measurement, the IPL, OPL, and RPE also showed a significant intensity reduction but at the later phase of dark adaptation compared to the ISe change (post-hoc tests using the Bonferroni correction: IPL at 20 min (p < 0.01); OPL at 15 min (p < 0.01); RPE at 20 min (p < 0.05)).

Figure 3
figure 3

Relative OCT intensity measurement of hyper-reflective bands in the retina. Data are represented as mean with 95% confidence intervals (error bars). Significant differences between time points were tested using a one-way repeated measures ANOVA with Bonferroni’s post-hoc tests. N = 14. Asterisks indicate the earliest time point showing a statistically significant difference (*p < 0.05) in each band compared to the baseline light-adapted measurement.

As a control experiment, we also tested potential anesthesia effects on intrinsic signal changes (Fig. 4). The ketamine–xylazine mixture is known to cause cardiodepressive effects that lower local blood flow and vessel diameter over time34,35. Since the effect is time-dependent, it is necessary to carefully manage the imaging time point from the anesthesia. Seven retinal OCT volumes were sequentially acquired over 30 min under ambient light conditions, and it took 8–10 min between the time of intraperitoneal injection and the first OCT volume acquisition. The results showed that there was no significant change in both ISe band intensity and outer retinal thickness (Fig. 4B,C). Collectively, dark adaptation induces significant IOS changes as well as outer retinal thinning.

Figure 4
figure 4

(A) Sequential OCT images obtained under ambient light conditions. (B) Relative OCT intensity measurement of the ISe band and (C) outer retinal thickness measurement under ambient light conditions. Data are represented as mean with 95% confidence intervals (error bars). N = 6. Scale bars: 100 µm.

High-speed monitoring of a light–dark transitional period

Notable retinal changes were observed even after 5 min dark adaptation (Figs. 2 and 3), likely suggesting that OCT may detect early changes of the retina during a short transitional period to darkness. High-speed B-scan recordings were thus performed with 62.5 ms temporal resolution at a fixed retinal plane. In a total 5 min recording, the light-adapted retina as a baseline was imaged for the first 30 s, and the retina was continuously being imaged for 4.5 min in darkness. The lights were off at 30 s. Figure 5A exhibits a motion scan (M-scan) illustrating spatiotemporal relations, where each column represents an average A-scan, and individual A-scans were vertically aligned based on the ELM’s peak location. Figure 5B shows the vertically enlarged view of the outer retina in Fig. 5A. In line with the previous results (Figs. 1 and 2), it was observed that the 3rd outer retinal band (i.e., IZ and OS tips) became elusive over time (white arrowheads in Fig. 5B), and the boundary between the RPE and choroid shifted toward the inner retinal side (green arrows in Fig. 5B), indicating the outer retina became short.

Figure 5
figure 5

High-speed OCT recording during the light–dark transition. The lights were off at 30 s. (A) Spatiotemporal mapping of OCT images. Each column represents a 1-s recording. (B) Enlarged view of the red box in (A). Black and white arrowheads point out the 3rd band change during dark adaptation. Green arrows indicate basal RPE position. ELM external limiting membrane, ISe inner segment ellipsoid, IS inner segment, OS outer segment, IZ interdigitation zone, RPE retinal pigment epithelium, CH Choroid.

Further detailed analysis was made by color-coded intensity profiles (Fig. 6A). Different colors represent different time points; dark red and blue indicate the early and late phases of recording, respectively. From the results, we found three imaging features. First, ISe intensity reduction rapidly occurred after lights off (Fig. 6B). Second, as seen in Fig. 5B, outer retinal bands, including the RPE and choroid, were being shifted toward the ELM side (Fig. 6C). These observations were consistent with the results presented in Figs. 2 and 3. Third, the color-coded intensity profiles delineate that the 3rd band decreased in contrast over time (Fig. 6D). Intriguingly, we observed not only a peak at the OS tip position (black arrows in Fig. 6A,D) but also another peak in the midway of the OS (green arrows in Fig. 6A,D), and both peaks tended to gradually decrease in intensity right after lights off. In addition, a linear trend between retinal responses and time courses of dark adaptation was estimated by Pearson correlation tests (Fig. 6E,F). Results showed that dark adaptation time was negatively correlated with ISe intensity (r = − 0.933) and ELM-RPE displacement (r = − 0.982). We also found a strong positive relationship between ISe intensity and ELM-RPE displacement (r =  + 0.944) (Fig. 6G). Collectively, these data suggest that retinal changes gradually occur in a linear manner upon darkness, and high-speed spatiotemporal mapping enables the measurement of prompt retinal activities due to dark adaptation.

Figure 6
figure 6

Quantitative analysis of high-speed OCT recording during transition to darkness. (A1-3) Color-coded OCT intensity profiles of the outer retina from 3 different mice. Red and blue colors indicate a temporal gradient of a 30-min dark adaptation period. The lights were off at 30 s. Green and black arrows indicate double peaks found along the photoreceptor OS. (B) Enlarged view of the ISe band, green box in (A1). (C) Enlarged view of the RPE and CH, grey box in (A1). (D) Enlarged view of the OS, IZ, and RPE, pink box in (A1). (E) Relative intensity changes and (F) ELM-RPE displacement during dark adaptation. Black dots represent the mean values, and the accompanied grey area represents standard deviation. (G) Correlation test between ISe intensity and ELM-RPE displacement. Black dots represent the mean values, and a red line is the linear fitting line. ELM: external limiting membrane; RPE: retinal pigment epithelium; ISe: inner segment ellipsoid; IS: inner segment; OS: outer segment; IZ: interdigitation zone; CH: Choroid. (AD) were generated using MATLAB R2018b (, and (EG) were generated using Origin 2020b (

Double OS peaks may represent cone and rod tips

An interesting observation in this study was a moderate-reflective peak above the conventional hyper-reflective 3rd band (i.e., rod IZ and OS tips) (Fig. 6A,D), suggesting the presence of an additional 3rd outer retinal band. Since this additional peak was consistently observed in 13 out of 14 mice, it was less likely an artifact. The first 3rd band (i.e., the ISe side, green arrows in Fig. 6A) was relatively gentle compared to the second 3rd band (i.e., the RPE side, black arrows in Fig. 6A), and both peaks gradually decreased in intensity during dark adaptation (Fig. 6A,D). A peak-to-peak distance was measured from the ISe to each 3rd band peak, and we found that the distances were 13.4 ± 1.8 μm for the first peak and 20.9 ± 2.2 μm for the second peak (Fig. 7A), well matching the cone and rod OS length in the mouse retina36. A representative M-scan image by averaging 14 different M-scans was also constructed to reveal a layered intensity distribution (Fig. 7B1). The distribution was not a continuous gradient along the depth direction; it was rather a discrete staircase distribution (Fig. 7B2). This pattern became more obvious when OCT intensity was depicted with a color-lookup-table (Fig. 7C). Collectively, our results demonstrate that the 3rd outer retinal bands in the mouse retina can be divided in two parts: the first moderate-reflective band and second hyper-reflective band.

Figure 7
figure 7

(A) Peak-to-peak length measurement from the ISe to double peaks along the photoreceptor OS (N = 13). (B1) An average M-scan image at the outer retinal region. (B2) A simplified intensity distribution model based on (B1). (C) OCT intensity in (B1) is depicted with a color-lookup-table “thal” provided in ImageJ software. The intensity range of the lookup table is shown in the bar to the right of the image. ISe inner segment ellipsoid, IS inner segment, OS outer segment, IZ interdigitation zone, RPE retinal pigment epithelium. (A) was generated using Origin 2020b ( (B1–B2) were generated using Microsoft PowerPoint 2019 ( (C) was generated using ImageJ 1.52v (


In this study, we demonstrated ORG measurement of dark adaptation in the mouse retina. Functional OCT as an ORG modality was sequentially collected before and after lights off to investigate retinal status under different light conditions. We found that retinal response during dark adaptation was reflected by transient structural (i.e., retinal thickness and OCT bands integrity) and physiological (i.e., intrinsic optical signals) changes, mainly in the outer retina. These responses were manifested as a function of the adaptation time and readily detectable upon darkness. We assumed that coherent interactions between the photoreceptors and RPE to be adapted in the absence of light may be directly or indirectly reflected in OCT imaging.

The altered reflectance observed in the ISe band can support the notion that metabolic states of the photoreceptors can be associated with intrinsic signal changes in the ISe37,38. The photoreceptors display the highest rate of oxidative metabolism in the body and regulate a flow of metabolic energy differentially in light and dark conditions. Energy demands and oxygen consumption are more significant in darkness than in light due to increased adenosine triphosphate (ATP) consumption to maintain the photoreceptor dark current6,39. This enhanced metabolism is mainly managed in the ISe, consisting of densely packed mitochondria. Due to complex internal geometry, mitochondria are the prominent light scatters in OCT imaging, and its geometrical configuration is actively modified based on metabolic demand through the fission and fusion process40. It is known that different mitochondrial configurations can alter the scattering property of light41,42, which may ultimately change the IOS property of the ISe. In line with this fact, fragmented mitochondria were frequently found in resting cells, while mitochondria became more fused when they were forced to rely on oxidative phosphorylation43. A recent study also demonstrated that mitochondria in the cone IS in hibernating ground squirrels were individually smaller and distorted, but mitochondria in active ground squirrels appeared elongated and well-organized. It was hypothesized that the elongated, parallel organization of mitochondria might enhance light delivery to the OS by reducing the number of refractive interfaces44. Taken together, it can be plausibly postulated that mitochondria in the ISe may undergo a fusion process during dark adaptation, forming tightly packed and elongated mitochondria to meet the high energy demand, resulting in low reflectance in the ISe band.

We also observed structural changes of the retina during dark adaptation. The mechanism of outer retinal thinning is rather well understood by RPE-mediated water removal from the ELM-RPE region. As discussed, the transition from light to dark is accompanied by an increase in photoreceptor metabolism, resulting in increased oxygen consumption in the retina45. The increase in oxygen consumption leads to a proportionate increase in CO2 and wastewater production into the outer retina, which can acidify the outer retina and ultimately upregulate water removal co-transporters in the RPE. This rapid removal of the acidified water has been linked to significant thinning of the ELM-RPE region46,47. In fact, empirically derived acidic conditions by medicine and hypoxia were found to reduce the outer retinal volume directly48,49. Consistent with our results, Li et al.28 observed a significant reduction of outer retina thickness in the dark-adapted retina. They also reported that light-dependent volume changes in the outer retina varied with the stage of retinal degeneration in retinal degeneration 10 (rd10) mouse model50. Berkowitz et al. found strain-specific changes of the outer retina in different light conditions. A light-driven expansion of the outer retina was more prominent in C57BL/6 mice than 129S6/SvEvTac mice29. Gao et al.30 further demonstrated that dark adaptation significantly reduced the magnitude and width of a hypo-reflective band between the photoreceptor OS and RPE. In addition, Guziewicz et al. showed that the hypo-reflective band became more profound during the transition from dark to light in the canine BEST1 disease model, manifesting retinal abnormalities at the photoreceptor-RPE interface associated with defects in the RPE microvilli ensheathment51. Taken together, dynamic ORG measurement of dark adaptation can serve as a functional indicator for RPE-mediated water transport in the outer retina.

However, the water transport could not explain the altered integrity of the 3rd outer retinal bands (i.e., IZ and OS tips). We instead hypothesize that interdigitating properties between the OS tip and RPE apical processes may be changed. In this study, we found double peaks along with the OS (Figs. 6A and 7), and these peaks became enervated during the dark adaptation period. This morphological configuration is quite similar to an axial positioning of cone and rod OS tips in the perifoveal outer retina in humans52,53,54,55. However, the putative cone IZ band has low reflectivity compared to the rod IZ band in the mouse retina, which is probably due to a sparse cone population. Considering the cone OS is shorter in length (Fig. 7A) and lower in occupancy than the rod OS in the mouse retina, the first and second peak most likely correspond to the cone and rod OS tip region, respectively. This ensheathing configuration has been also named as the contact cylinder53, where the RPE’s long apical microvilli interdigitate with the photoreceptor OSs, supporting an adhesion between the retina and RPE. Microvilli are capable of active contraction while interdigitating with the OS51. It was demonstrated that the force required to detach the retina from the RPE was 20% greater in the light-adapted retina than in the dark-adapted retina56. Also, retinal adhesion in wild-type mice increased by 58% after light adaptation57. To support the firm retinal attachment, tight ensheathment might be necessary for light adaptation, presumably manifesting high reflectance in the 3rd band. Conversely, the alleviated tension in darkness along with outer retinal thinning may loosen this interconnection, presumably manifesting low reflectance in the 3rd band. It should be noted that ensheathment of the OS by microvilli is not the only mechanism of retinal adhesion; interphotoreceptor matrix proteins simultaneously contribute to lowering retinal adhesive force in darkness5,58. In addition, either light modulated melanosome translocation59 or rearrangement between OS and microvilli60 during dark adaptation can also be a contributing factor in the 3rd IZ and OS tip band signal. In fact, the 3rd band is the most controversial band among researchers, and multiple interpretations for this region have been proposed54,61,62,63. Thus, the exact mechanism remains to be extensively investigated.

In conclusion, we demonstrated the feasibility of in vivo ORG assessment of dark adaptation to characterize dark-induced structural and reflectivity changes in the mouse retina. Compared to the conventional perimetry and ERG measurements, functional OCT enables intrinsic signal ORG dark adaptation kinetics in a quantitative way with layer-specificity. Moreover, the rapid testing protocol of dark adaptation kinetics would be suitable for both lab research and clinical application because of low subject burden. Although the complex correlations of homeostatic mechanisms and imaging features remain to be elucidated, the intrinsic signal ORG promises a great potential to enable noninvasive, objective measurement of dark adaptation kinetics, which can aid in the diagnosis of early-stage AMD, DR, and RP.


Animal preparation

Two-month-old C57BL/6 J mice of either sex (Jackson Laboratory, Bar Harbor, Maine, USA) were used in this study. The mice were kept in regular animal housing under a 14:10 h light/dark cycle. All animal experiments were approved by the local animal care and biosafety office and performed following the protocols approved by the Animal Care Committee (ACC) at the University of Illinois at Chicago. This study followed the Association for Research in Vision and Ophthalmology Statement for the Use of Animals in Ophthalmic and Vision Research, and we confirm that our work accords with the ARRIVE guidelines.

Imaging system

A custom-designed spectral-domain OCT system was used in this study. Technical details of the system are reported in our previous studies64,65. Briefly, a near-infrared super-luminescent diode (λ = 810 nm; Δλ = 100 nm; D-810-HP, Superlum, Carrigtwohill, County Cork, Ireland) was used as the OCT light source. A line CCD camera with 2048 pixels (AViiVA EM4; e2v Technologies, Chelmsford, UK) was used in the custom-built OCT spectrometer. The axial and lateral resolutions of the system were theoretically estimated at 2.9 and 11 μm, respectively. The incident light power on the cornea was 1 mW.

Experimental procedure

Imaging was performed in a laboratory room at ~ 400 lx. Stray light from electronic equipment was masked, and the imaging stage was shrouded with blackout fabric. Experiments were regularly performed between 12 and 3 pm. At the time of the experiment, the mouse retina was light-adapted due to constant ambient light conditions. The mouse was first anesthetized by intraperitoneal injection of a mixture of 100 mg/kg ketamine and 5 mg/kg xylazine. The anesthesia cocktail concentration was 10 mg/mL ketamine and 0.5 mg/mL xylazine. A drop of 1% tropicamide ophthalmic solution (Akorn, Lake Forest, IL) was applied to the imaging eye for pupil dilation, and a cover glass (12-545-80; Microscope cover glass, Fisherbrand, Waltham, MA) with a drop of eye gel (GenTeal, Novartis, Basel, Switzerland) was placed on the imaging eye. After the mouse was fully anesthetized, the head was fixed by a bite bar and ear bars in an animal holder. A heating pad was placed around the animal holder to keep the mouse warm. It took around 8–10 min between the time of intraperitoneal injection and the first OCT volume acquisition.

Image acquisition

Volumetric raster scans were performed for OCT imaging. Four repeated B-scans at each slow-scan position were collected and averaged to lower the speckle noise level; thus, each OCT volume consisted of 4 × 600 × 600 A-scans over 1.2 × 1.2 mm area. Seven OCT volumes were sequentially acquired with 5 min intervals at the same dorsal quadrant. Figure 8 illustrates the flow of image acquisition. The first volume was acquired in light condition, and the other six volumes were acquired in dark condition. High-speed recording to capture the initial moment of dark adaptation was conducted right after the first volume recording. The high-speed recording captured 4800 B-scans at the same retinal plane with 16 frames per second rate for a complete 5-min recording. While recording, the room light was turned off at 30 s with the guidance of a LED blink synchronized with the OCT image acquisition system. Data acquisition and real-time imaging preview were made by a custom-built LabVIEW program (LabVIEW 2013, National Instruments, Austin, TX).

Figure 8
figure 8

Image acquisition flow chart. The first OCT volume was acquired under ambient light conditions. After lights off, the second to seventh OCT volumes were sequentially acquired every 5 min up to 30 min. In between the first and second OCT volume acquisition, high-speed B-scan recording was performed to monitor retinal response during a light–dark transitional moment.

Image analysis

For quantitative analysis, retinal flattening was first implemented by realigning all A-lines in each OCT volume66. Next, adjacent 150 B-scans at the central region of OCT volume were averaged, followed by averaging 200 central A-lines, returning a single representative A-line per volume. The central region was carefully designated based on structural landmarks in each volume to secure regional consistency. Based on this A-line profile, retinal thickness and relative intensity value were measured. Each intensity profile was normalized based on the ONL intensity before relative intensity measurement in seven different OCT volumes. For the high-speed recording analysis, every 16 B-scans were first averaged, followed by retinal flattening, which returned a total 300 B-scans, and each B-scan represents 1-s recording. 200 central A-lines were averaged for intensity and displacement measurement. To enhance the sampling density, each A-line was four times upsampled using linear interpolation. Image reconstruction and processing were done by MATLAB R2016a (MathWorks, Natick, MA).

Statistical analysis

Data are expressed as mean ± standard deviation unless otherwise indicated. Statistical analysis was carried out with one-way repeated measures ANOVA to compare OCT measurements between seven different time points, followed by post hoc tests using Bonferroni correction to detect significant differences between the time points. The p-values less than 0.05 were considered significant in all tests. For high-speed recording analysis, Pearson's correlation analysis was conducted to examine the strength of a linear relationship between OCT measurements at different time points. All statistical analyses were performed with Origin 2020b (OriginLab, Northampton, MA).