Statistical estimation of deltoid subcutaneous fat pad thickness: implications for needle length for vaccination

Current US Centers for Disease Control and Prevention intramuscular injection needle length guidelines for injection fo the deltoid muscle are based on weight and gender. The aims of this study are (1) to evaluate whether other biometric data (age, gender, height, weight and body mass index (BMI)) are better predictors of the thickness of the deltoid subcutaneous fat pad (DSFP) than weight and gender and (2) to evaluate the performance of the CDC weight-based needle length guidelines. This was a retrospective single center cohort study of 386 patients who underwent surveillance PET/CT between 01/01/2020 and 04/01/2021. Patient age, gender, height, weight, BMI and CT measurements of the DSFP were evaluated. DSFP was positively correlated with weight and BMI in men (r = 0.67, P < 0.001; r = 0.74, P < 0.001) and women (r = 0.69, P < 0.001; r = 0.75, P < 0.001) respectively. DSFP was negatively correlated with age in women (r =  − 0.19, P = 0.013). Age and BMI were better predictors of DSFP than weight. The best model to predict the DSFP is: \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$DSFP in mm= 6.267199 -2.711159*Male + 0.228407*BMI + 0.012295*{BMI}^{2} -0.294211*BMI*Male -0.110063*Age + 0.085589*Age*Male$$\end{document}DSFPinmm=6.267199-2.711159∗Male+0.228407∗BMI+0.012295∗BMI2-0.294211∗BMI∗Male-0.110063∗Age+0.085589∗Age∗Male A 1-inch needle is expected to reach the deltoid in 85.3% of women less than 200 pounds, and 98.6% of men less than 260 pounds. This rate differed between genders (P < 0.001, odds ratio (OR) 0.08, 95% CI (0.02, 0.29)). A 1.5-inch needle is expected to reach the deltoid in 76.7% of women greater than 200 pounds, and 75.0% of men greater than 260 pounds. Current CDC deltoid intramuscular injection needle length guidelines result in women and obese individuals being more likely to receive subcutaneous injections. Age and BMI based guidelines for needle length selection are more accurate.

Vaccines are one of the most effect tools available for preventing infectious diseases. Vaccine administration requires appropriate technique, including appropriate selection of needle length to administer the vaccine in the intended location. Intramuscular vaccines including Hepatitis A, Hepatitis B, Rabies, Influenza, Pneumococcal, Diptheria toxoid and Coronavirus-19 (COVID-19), are to be administered intramuscularly to have maximum efficacy, and are usually within the deltoid muscle 1,2 . Inappropriate (too short) needle length has been shown to be associated with subcutaneous administration of the vaccine injectate [3][4][5][6][7][8] . Subcutaneous administration of injectate results in decreased vaccine efficacy 1,2 and has been shown to lead to decreased seropositivity and decreased antibody titers compared to intramuscular injections [3][4][5]9 .
Several studies have tried to predict the optimal needle length for deltoid intramuscular injections 10,11 . The optimal needle length required for a deltoid intramuscular injection required estimation of the distance from the skin overlying the deltoid muscle to the deltoid muscle, which is the deltoid subcutaneous fat pad (DSFP) thickness and the distance from the skin overlying the deltoid to the humerus 10,11 . A study by Poland et al. evaluated the thickness of the deltoid subcutaneous fat pad (DSFP) in 220 healthy healthcare workers using ultrasound and showed that the DSFP varied by gender and weight, with heavier individuals having a thicker DSFP 10 . This study was the basis for the current United States Centers for Disease Control and Prevention (CDC) needle length guidelines for deltoid intramuscular injections. Current CDC guidelines recommend a needle length of 1 inch for women weighing less than 200 pounds, and for men weighing less than 260 pounds; and recommend a needle length of 1.5 inches for women weighing 200 pounds or more, and for men weighing 260 pounds or more 10 . The current CDC guidelines are based on gender and weight only. This means that the recommended We hypothesized that the CDC guidelines may be inadequate for clinical practice as they rely on weight, rather than on BMI. The goal of this study is to use statistical modeling using patient clinical and demographic factors (age, gender, height, weight and BMI) to predict DSFP thickness and to better predict the optimal needle length required to achieve an intramuscular deltoid injection.

Methods
The study experimental protocol was reviewed and approved by the local Institutional Review Board at the Mayo Clinic, Florida (IRB approval # 21-003745), and the need for signed informed consent from each patient was waived. All methods were carried out in accordance with the Health Insurance Portability and Accountability Act of 1996 (HIPAA) guidelines and regulations.
Prior reports have measured the DSFP using ultrasound and tried to avoid compression of the deltoid tissues by using "light" probe pressure or used a "stand-off pad" to increase the near field resolution of the skin line 10,11 . It is unclear how much pressure is "light" pressure and how much this "light" pressure could distort the subcutaneous fat, since by nature fat is soft and easily compressible. Ultrasound by nature of its compressive technique often distorts/compresses the subcutaneous tissues 10,11 , so ultrasound was not chosen as the modality to obtain measurements for this study. Magnetic resonance imaging (MRIs) of the shoulder were considered, however, MRIs of the shoulder utilize a shoulder coil. This shoulder coil also potentially compresses the subcutaneous tissues, and so MRIs were not chosen as the modality to obtain measurements. Chest computed tomography (CTs) were considered as these studies often include the shoulder, however, the arms are generally held above the head to minimize artifacts and radiation dose to the patient, so chest CTs were not utilized. The CT component of whole-body positron emission tomography/computed tomography (PET/CTs) often includes the shoulder in the anatomic position and generally have no mass effect on the subcutaneous tissues because the patient is lying supine, so this modality was chosen to obtain measurements.

DSFP measurement technique.
A fellowship-trained musculoskeletal radiologist with over 12 years of experience evaluated scans and made measurements on the left arm in all but two patients who had intramuscular lipomas in the deltoid. The left arm was chosen for measurements because it is the common non-dominant arm and usually chosen for deltoid injections. The distance from the skin overlying the deltoid to the deltoid muscle, d (equivalent to the DSFP); and the distance from the skin overlying the deltoid to the humerus, D, were measured at exactly 5 cm inferior to the acromion in the mid anterior-posterior shoulder at 90° perpendicular to the skin measured in the coronal plane, following the CDC guidelines 12 (Fig. 1). Two millimeters of the needle was assumed to be above the skin to facilitate retrieval of the needle in the event of a fractured needle. A previous manuscript noted that an injection would be considered intramuscular if the needle would penetrate the deltoid muscle by at least 5 mm 10 , so this threshold was used.
Statistical analyses. T-tests were used to compare mean weights and mean DSFP thickness for men and women. Pearson's correlation coefficients were used to evaluate the correlations between DSFP thickness and weight, BMI, age and height for men and women. Receiver operator characteristic (ROC) curves were used to evaluate the predictive performance of age, height, weight, and BMI to predict whether injections were intramuscular versus subcutaneous using the CDC current guidelines. DeLong's test was used to compare ROC curves. Next, logistic regression was used to predict the odds of an inadvertent subcutaneous model using the CDC guidelines, comparing patients above the CDC weight threshold (200 pounds for women, 260 pounds for men) compared to patients below the CDC weight threshold.
Predictive statistical modeling was used to estimate a patient's DSFP based on age, gender, height, weight, and BMI using generalized linear models. The Shapiro-Wilks test was performed to confirm the distribution of DSFP was not significantly different from a normal distribution. First, univariate linear regression models to predict DSFP using age, height, weight and BMI as covariates were created. Age (P < 0.001), height (P < 0.001), weight (P < 0.001) and BMI(P < 0.001) were significant univariate predictors of DSFP.
We built the best multivariable model to predict DSFP using age, gender, height, and weight (model 1). Variables with P-values < 0.20 in the univariate analysis were included in the first multivariable model, and we removed terms with P-values ≥ 0.05 in this multivariable model using stepwise backward regression. We We built the best multivariable model to predict DSFP using age, gender, height, and BMI (model 2). Variables with P-values < 0.20 in the univariate analysis were included in the first multivariable model, and we removed terms with P-values ≥ 0.05 in this multivariable model using stepwise backward regression. We subsequently included all squared terms for each remaining variable to the multivariable model. Variables which were not statistically significant (P ≥ 0.05) were removed from the model using stepwise backward regression. Next, all pairwise interaction terms between the remaining variables in the multivariable model were added to the multivariable model. Variables which were not statistically significant (P ≥ 0.05) were removed from the model using stepwise backward regression. The final model 2 included the following variables: age, gender, BMI, BMI-squared, gender*BMI interaction term, age and age*gender interaction term (Adjusted R-squared = 0.648, AIC = 2204.1). Model 2 was compared to model 1 using the Akaike Information Criterion (AIC). Model 2 was superior to model 1 and was used for predictive modeling.
The best model to predict the DSFP is: Model 2 (Eq. 1) was used to create plots of the predicted thickness of the DSFP in men and women aged 35 and 75 years with varying BMIs (Figure iv). Statistical analyses were conducted using Rv4.04. All tests were two-sided and P-values less than 0.05 were considered statistically significant.   3) mm). DSFP was positively correlated with weight in men (r = 0.67, P < 0.001) and women (r = 0.69, P < 0.001); and with BMI in men (r = 0.74, P < 0.001) and women (r = 0.75, P < 0.001). DSFP was negatively correlated with age in women (r = − 0.19, P = 0.013) and borderline negatively correlated with age in men (r = − 0.13, P = 0.054). We found no correlation between DSFP and height in either men (r = − 0.04, P = 0.601) or women (r = − 0.03, P = 0.691).
Our results show that a 1-inch needle is expected to reach the deltoid in 85.3% (116/136) of women less than 200 pounds, and 98.6% (207/210) of men less than 260 pounds. This rate differed between genders (P < 0.001, odds ratio (OR) 0.08, 95% CI (0.02, 0.29)). A 1.5-inch needle is expected to reach the deltoid in 76.7% (23/30) of women greater than 200 pounds, and 75.0% (6/8) of men greater than 260 pounds (Fig. 2). The odds of inadvertent subcutaneous injection in men greater than 260 pounds and women greater than 200 pounds (above the CDC weight thresholds) are over 4 times higher than those below the threshold (OR = 4.33, P = 0.002) when using the CDC-recommended needle lengths. BMI was the best predictor of DSFP in men but was not significantly better than weight as a predictor in women (Fig. 3, Supplementary Fig. 1). For women, the area under the receiver-operator curve (ROC) curve (AUC) for BMI (0.767) to predict an intramuscular injection was significantly better than the AUC for height (AUC = 0.527, P < 0.001) and the AUC for age (AUC = 0.562, P < 0.001) but was not significantly different from the AUC for weight (AUC = 0.750, P = 0.210). For men, we found that the AUC for BMI (AUC = 0.832) to predict an intramuscular injection was significantly better than the AUC for weight (AUC = 0.777, P = 0.001), the AUC for height (AUC = 0.546, P < 0.001) and the AUC for age (AUC = 0.612, P < 0.001).
The best model to predict the DSFP is: We used this model to predict the DSFP thickness for males (aged 30 and 75 years) and females (aged 30 and 75 years) with varying BMI (Fig. 4).

Discussion
In this study, we show that both weight and BMI were strongly correlated with DSFP in men and women. Despite weighing less on average than men, women tended to have thicker DSFPs than men. In our predictive modeling, we found that age, gender and BMI were more predictive of DSFP than the best model using age, gender and weight.
A deltoid intramuscular injection has been defined as an injection with penetration of the muscle by 5 mm or more, with 2 mm of needle superficial to the skin to aid in needle retrieval in the event of an accidental needle break 10 . Based on this, we conclude that the DFSP can be predicted using the following equation:  www.nature.com/scientificreports/ The predicted DSFP can then be used to find the appropriate needle length where the appropriate needle length is predicted DSFP + 7 mm. Therefore, if the predicted DSFP < 18.4 mm, then a 1-inch (25.4 mm) needle would be appropriate. Similarly, if the predicted DSFP > 18.4 mm but < 31.1 mm, then a 1.5-inch (38.1 mm) needle would be appropriate. Because of this, a 1-inch needle may be too short to reliably get to the deltoid muscle in women, particularly in heavier women and a 1.5-inch needle may be too short to achieve an intramuscular injection in women greater than 200 pounds, and men greater than 260 pounds. Age and BMI together are better than weight for predicting DSFP thickness. DSFP decreases with age faster in women.
More recently new vaccines have been developed for COVID-19 in the wake of the COVID-19 pandemic. Currently, there is a global effort to vaccinate individuals with these new COVID-19 vaccines. This effort requires appropriate needle length selection for vaccination administration. Our results suggest that women, and in particular younger and obese women would be more likely to have inadvertent subcutaneous injections and be at increased risk for vaccine failures. Recent CDC Morbidity and Mortality Weekly Report (MMWR) data show that 63% of COVID-19 vaccine failures have been in women 13 , however, the relative proportions of males to females in the vaccinated population is unknown.
While this study and other studies have shown associations between the DSFP and weight and BMI 10,11 , there are a few differences between this study and previously published reports. The prior report by Poland et al. investigated healthcare workers aged 18-59 years, weight range of 59-118 kg and BMI of 17.1-49.9 kg/m 2 whereas this study investigated patients aged 19-93 years, weight range of 31.9-158 kg and BMI of 13.3-55.9 kg/ m 2 . This study has individuals over a greater age range, weight range and BMI range than the study by Poland et al. 10 . Similarly, Cook et al. 11 conclude that a 1-inch needle (25.4 mm) is an appropriate length for men of all BMI, whereas this study showed that this needle length was appropriate for 96.8% of men of all BMI (211/218) and only appropriate for 37.5% (3/8) men who weighed more than 260 pounds. This is likely because our study had larger weight and BMI ranges than these prior studies. Poland et al. suggest that a 5/8 inch, 1-inch (25.4 mm) and 1.5-inch (38.1 mm) needle is appropriate for women less than 60 kg, 60-90 kg and greater than 90 kg respectively, which contradicts the finding by Cook et al. who suggested that a 1-inch needle is appropriate for  www.nature.com/scientificreports/ all women with BMI < 35 11 . Our data suggest that the appropriate needle length for a woman is more complicated and depends on her age and BMI. This study utilizes CT rather than ultrasound for measuring DSFP. Prior reports used ultrasound to measure the DSFP 10,11 . The advantage of using ultrasound is that it does not involve radiation, and it can be used prospectively to evaluate patients. However, ultrasound compresses the subcutaneous tissues 10,11 and in our experience this compression likely results in underestimation of the DSFP. The advantage of using CT is that the DFSP measurement is unaffected by compressive forces on the subcutaneous tissues 6-8 , however one disadvantage of CT is that the radiation would make it difficult to prospectively evaluate healthy individuals.
One interesting finding that we have noted is that there seem to be differences in the adiposity patterns between males and females. The prior study by Cook et al. 11 noted that females with the same BMI as males had significantly thicker subcutaneous layers (P < 0.001) and thinner muscle layers (P < 0.001). Poland et al. also noted on average thicker DSFPs in women compared to men 10 . Our analysis confirms these findings. We also find that there was a statistically significant interaction between age and gender, with the DSFP decreasing at a faster rate in women than men with aging. Prior studies have shown that with age, there is increasing internal adiposity resulting in an increase in BMI, while simultaneously a decreasing in subcutaneous fat 14 . This change is thought to be related to adipose tissue shifts from primarily subcutaneous depots to visceral depots, which are more closely associated with the development of metabolic syndrome/insulin resistance 14 . This redistribution of adipose tissue also results in sunken cheeks, prominence of wrinkles and thinning of the skin over the hands and legs due to subcutaneous fat loss 14 . Further research is required to understand the differences in whole body adiposity patterns between males and females, and to understand how these differences change with age.
This study has a few limitations. One limitation is the study sample size; however, this study is 75% larger than the study used in creating the CDC needle length guidelines 10 . There are several additional factors that may contribute to vaccine failure, including an individual's genetic makeup, pathogen genetic variants (viral and bacterial genetic variants), injection technique including education and training of personnel who administer vaccines, and injection location. This study used the injection guidelines proposed by the CDC, which may or may not be adhered to in clinical practice. Another limitation of this study is that most of the patients underwent PET/CT for surveillance imaging for malignancy. It is unclear how a history of malignancy could affect the DSFP. However, our study population is an important population because most early deaths related to COVID-19 in the United States were related to patients with medical comorbidities including cancer and obesity 15 . Finally, most of the patients in our study were White individuals of European ancestry. It is unclear whether these results port to members of all races/ethnicities equally.
In summary, current CDC guidelines for deltoid intramuscular injections may result in subcutaneous injection and potentially decreased vaccine efficacy in women and obese individuals.