The virucidal effects of 405 nm visible light on SARS-CoV-2 and influenza A virus

The germicidal potential of specific wavelengths within the electromagnetic spectrum is an area of growing interest. While ultra-violet (UV) based technologies have shown satisfactory virucidal potential, the photo-toxicity in humans coupled with UV associated polymer degradation limit their use in occupied spaces. Alternatively, longer wavelengths with less irradiation energy such as visible light (405 nm) have largely been explored in the context of bactericidal and fungicidal applications. Such studies indicated that 405 nm mediated inactivation is caused by the absorbance of porphyrins within the organism creating reactive oxygen species which result in free radical damage to its DNA and disruption of cellular functions. The virucidal potential of visible-light based technologies has been largely unexplored and speculated to be ineffective given the lack of porphyrins in viruses. The current study demonstrated increased susceptibility of lipid-enveloped respiratory pathogens of importance such as SARS-CoV-2 (causative agent of COVID-19) and influenza A virus to 405 nm, visible light in the absence of exogenous photosensitizers thereby indicating a potential alternative porphyrin-independent mechanism of visible light mediated viral inactivation. These results were obtained using less than expected irradiance levels which are considered safe for humans and commercially achievable. Our results support further exploration of the use of visible light technology for the application of continuous decontamination in occupied areas within hospitals and/or infectious disease laboratories, specifically for the inactivation of respiratory pathogens such as SARS-CoV-2 and Influenza A.

www.nature.com/scientificreports/ Taking this information into consideration, several methods have been shown to effectively inactivate SARS-CoV-2. Chemical methods, which focus on surface disinfection, utilize 70% alcohol and bleach and their benefits are well established. These methods are also episodic (or non-continuous) meaning that in-between applications, the environment is not being treated 13 .
In addition to chemicals, one of the most utilized methods for whole-room disinfection is germicidal ultraviolet C (UVC; ~ 254 nm) 14 . This technology is well established 15 and has been shown to inactivate a range of pathogens including bacteria 16 , fungi 17 and viruses 18 . The mechanism of action of UVC is photodimerization of genetic material such as RNA (relevant for SARS-CoV2 and IAV) and DNA (relevant for DNA viruses and bacterial pathogens, among others) 19 . Unfortunately, this effect has been associated with deleterious effects in exposed humans such as photokeratoconunctivitis in eyes and photodermatitis in skin 20 . For these reasons, UVC irradiation requires safety precautions and cannot be used to decontaminate fomites and high contact areas in the presence of humans 21 .
Germicidal properties of violet-blue visible light (380-500 nm), especially within the range of 405 to 450 nm have been shown as an alternative to UVC irradiation in human occupied whole-room disinfection scenarios where it has shown reduction of bacteria 22,23 and surgical site infections 24 . Although 405 nm or closely related wavelengths have been shown to be less germicidal than UVC, its inactivation potential has been assessed in pathogenic bacteria such as Listeria spp. and Clostridium spp. 24,25 , and in fungal species such as Saccharomyces spp. and Candida spp. 26 . It is thought that the underlying mechanism of blue-light mediated inactivation is associated with absorption of light via photosensitizers such as porphyrins which results in the release of reactive oxygen species (ROS) 27,28 . The emergence of ROS is associated with direct damage to biomolecules such as proteins, lipids and nucleic acids which are essential constituents of bacteria, fungi and viruses. Further studies have shown that ROS can also lead to the loss of cell membrane permeability mediated by lipid oxidation 29 . Given the lack of endogenous photosensitizers such as porphyrins in virions, it was expected that efficient decontamination of viruses (both enveloped and non-enveloped) required the addition of exogenous photosensitizers 23 . With the use of media suspensions (e.g. saliva, blood, etc.) containing both endogenous and/or exogenous photosensitizers (to create the ROS required for inactivation), 405nm light was shown to inactivate viruses such as feline calcivirus (FCV) 30 , viral hemorrhagic septicemia virus (VHSV) 31 and murine norovirus-1 32 using unsafe, commercially impractical irradiance levels. This highlights the importance of answering the basic scientific questions related to viral inactivation within the context of the applied science required for clinical application. Our study specifically focused on three questions: (1) does the same wavelength of light (405 nm) that inactivates bacteria also inactivate enveloped viruses, (2) is a porphyrin rich medium required for this inactivation, and (3) can this inactivation be achieved using safe, commercially practical irradiance levels?
In the current study, we show the impact of 405 nm irradiation on inactivation of SARS-CoV-2 and influenza A H1N1 viruses without the use of photosensitizers making it directly relevant to the clinical environment. We show this using a commercially available visible light disinfection system ensuring that the irradiance used is both safe and practically achievable in a clinical setting supporting the possible use of 405 nm irradiation as a tool to confer continuous decontamination of respiratory pathogens such as SARS-CoV-2 and influenza A viruses. We further show the increased susceptibility of lipid-enveloped viruses for irradiation in comparison to non-enveloped viruses, further characterizing the virucidal effects of visible light.
Materials and methods 405 nm exposure system. The visible light disinfection product used in this study was a commercially available 6″ LED downlight (Indigo-Clean, Kenall, Kenosha, WI) to allow for use within a BSL-3 level containment hood. Within the hood, the distance between the face of the fixture and the sample was 10″-much less than the normal 1.5 m used in normal, whole-room disinfection applications. The output of the fixture was modified electronically during its manufacture to match this difference and ensure that the measurements would represent the performance of the device in actual use. This test setup is shown in a BSL-2 hood in Fig. 1A below. Note the spectroradiometer and the bottom portion of the rig (~ 6″) used for calibration were removed during the actual study (Fig. 1B).
Controls were placed outside the test rig but within the BSL hood as shown in Fig. 1B. Note that this picture contains the bottom portion of the test rig to highlight the position of the radiometer. For the range of output used in this study, multiple discrete levels were created using pulse width modulation within the LED driver itself. These levels were made to be individually selectable using a simple knob on the attached control module.
For reference, the amount of visible light within the 400-420 nm bandwidth, measured in mW cm −2 , is a measurement of the "dose" delivered to the target organism and is used to quantify this relationship similar to that used in UV disinfection applications.
To fully examine this effect, a range of irradiance values were used representing actual product deployment conditions in occupied rooms. The lowest value (0.035 mW cm −2 ) represents a single-mode, lower wattage used in general lighting applications while the highest value (0.6 mW cm −2 ) represents a dual-mode, higher wattage used in critical care applications such as an operating room.
The device was placed in a rig to ensure a consistent distance (10″) between the fixture and the samples. The output of the fixture in the test rig was measured using a Stellar-RAD Radiometer from StellarNet configured to make wavelength and irradiance measurements from 350 to 1100 nm with < 1 nm spectral bandwidth using a NIST traceable calibration. To ensure that the regular white light portion of the illumination (which is nondisinfecting) was not measured, the measurement was electronically limited to a 1 nm bandwidth over the 400-420 nm range. The normalized spectral profile is shown in Fig. 1C below. The absolute value of the measurement was determined using a NIST traceable calibration as previously described. Samples were irradiated with the range of wavelengths depicted in Fig. 1C. This was deliberately done for two reasons: (1) prior work  (2) to emphasize the applied science associated with actual clinical use where a virus in the environment could be exposed to both 405 nm and regular white light in an occupied room.
To isolate the contribution of 405 nm light, the control samples were placed outside the field of irradiation created by the disinfection product but within the biosafety hood. Accordingly, these samples were exposed to the overhead lights within the room which contained virtually no 405 nm light (< 0.001 mW cm −2 ).
In any assessment of viral inactivation, thermal denaturing of the organism is a concern. Older lighting technologies such as incandescent sources heat a resistive element and were widely used in a variety of applications. This creates heat at both the source and to objects within its field. Fortunately, the disinfecting light (sample) and the overhead lights in the room (control) did not use this technology and therefore contain no infrared emissions (> 800 nm) a commonly known benefit associated with LED lighting. As a confirmation, the temperature beneath the disinfecting light was measured using a commercially available thermocouple during a 24-h period. During this time, the temperature at the sample position was constant at 20 °C ± 0.5 °C even at the highest disinfecting power (0.6 mW cm −2 ).
For reference, two spectral profiles, one for traditional fluorescent lighting and the other for standard LED lighting are provided in Fig. 4 below. While both types of lighting are commonly used in the overhead lights within buildings, the lights in the BSL-3 laboratory were traditional fluorescent. As shown in Fig. 1B, the controls were exposed only to traditional fluorescent lighting with a negligible amount of disinfecting light (< 0.001 mW cm −2 ) between 400 and 420 nm. Due to the inherent differences between fluorescent and LED lighting, the standard LED spectra has a small, but measurable amount of disinfecting light (0.006 mW cm −2 ) between 400 and 420 nm. As will be later shown, this amount of light can have a measurable disinfecting effect. The bottom portion of the test rig was removed during the actual experiment to ensure the 10″ distance used in the study. (C) Normalized spectral power distribution showing peak irradiance at 405 nm. (D) Normalized spectral power distribution for the fluorescent control light (non-disinfecting) and standard LED light (without 405 nm) used in the study. Each spectrum is normalized relative to its own peak value. All experiments involving SARS-CoV2 (USA-WA1/202, BEI resource-NR52281) were conducted within a biosafety-level 3 (BSL3) containment facility at Icahn school of medicine at Mount Sinai by trained workers upon authorization of protocols by a biosafety committee. Amplification of SARS-CoV-2 viral stocks was done in Vero-E6 cell confluent monolayers by using an infection medium composed of DMEM supplemented with 2% HI-FBS, Non-essential amino acids (NEAA), Hepes and penicillin-streptomycin at 37 °C with 5% CO 2 for 72 h. Influenza A virus used here was generated using plasmid based reverse genetics system as previously described 34 . The backbone used in the study was A/Puerto Rico/8/34/Mount Sinai (H1N1) under the GenBank accession number AF389122. IAV-PR8 virus was grown and titrated in MDCK as previously described 34 . As a non-enveloped virus, the cell culture adapted murine Encephalomyocarditis virus (EMCV; ATCC ® VR-12B) was propagated and titrated in Vero-CCL81 cells with DMEM and 2% HI-FBS and penicillin-streptomycin at 37 °C with 5% CO 2 for 48 h 35 .

nm inactivation of viruses.
The SARS-CoV-2 virus was exclusively handled at the Icahn school of Medicine BSL-3 and studies involving IAV and EMCV were handled in BSL-2 conditions. Indicated PFU amounts were mixed with sterile 1× PBS and were irradiated in 96 well format cell culture plates in triplicates.
In these studies, A starting dose of 5 × 10 5 PFU for SARS-CoV-2 and starting doses of 1 × 10 5 PFU for IAV and EMCV were used. The final volumes for inactivation were 250 μl per replicate. The untreated samples were prepared the same way and were left inside the biosafety cabinet isolated from the inactivation device at room temperature. The plates were sealed with qPCR plate transparent seal and an approximate 10% reduction of the intensity was observed due to the sealing film. The distance from the lamp and the samples was measured to be 10″. All samples were extracted at indicated times and were frozen at − 80 °C and were thawed together for titration via plaque assays.
Plaque assays. For SARS-CoV-2 studies, confluent monolayers of Vero-E6 cells in 12-well plate format were infected (with an inoculum volume 150 μl) with tenfold serially diluted samples in 1× phosphate-buffered saline (PBS) supplemented with bovine serum albumin (BSA) and penicillin-streptomycin for an hour while gently shaking the plates every 15 min. Afterwards, the inoculum was removed, and the cells were incubated with an overlay composed of MEM with 2% FBS and 0.05% Oxoid agar for 72 h at 37 °C with 5% CO 2 . The plates were subsequently fixed using 10% formaldehyde overnight and the formaldehyde was removed along with the overlay. Fixed monolayers were blocked with 5% milk in Tris-buffered saline with 0.1% tween-20 (TBS-T) for an hour. Afterwards, plates were immunostained using a monoclonal antibody against SARS-CoV2 nucleoprotein (Creative-Biolabs; NP1C7C7) at a dilution of 1:1000 followed by 1:5000 anti-mouse IgG monoclonal antibody and was developed using KPL TrueBlue peroxidase substrate for 10 min (Seracare; 5510-0030). After washing the plates with distilled water, the number of a plaques were counted. Plaque assays for IAV and EMCV were done in a similar fashion. For IAV, confluent monolayers of MDCK cells supplemented with MEM-based overlay with TPCK-treated trypsin was used and was incubated for 48 h at 37 °C with 5% CO 2 . For EMCV, Vero-CCL81 cells were used to do plaque assays in 6 well plate format with an inoculum volume of 200 μl and was incubated for 48 h at 37 °C with 5% CO 2 . Plaques for IAV and EMCV were visualized using crystal violet. Data shown here is derived from three independent experimental setups.

Results
Dose and time dependent inactivation of SARS-CoV-2 in the absence of photosensitizers. The lowest irradiation dose of 0.035 mW cm −2 was applied for SARS-CoV-2 and when compared to the T 4H untreated control, a reduction of 0.3288 log 10 was seen as early as 4 h and after 24 h of irradiation, an inactivation of 1.0325 log 10 (approximately 10 times reduction in infectivity) was observed for SARS-CoV-2 via plaque assays ( Fig. 2A). A slightly higher dose of 0.076 mW cm −2 yielded 0.4123 log 10 , 0.6118 log 10 and 1.5393 log 10 reduction by 4, 8 and 12 h post irradiation when compared to the respective untreated controls (Fig. 2B). Subsequent increase of the irradiation dose to 0.150 mW cm −2 resulted in a reduction of 0.4771 log 10 after 4 h which then had a 1.1206 log 10 after 12 h. Irradiation for 24 h at 0.150 mW cm −2 suggested a total reduction of 2.0056 log 10 (256 times) for SARS-CoV-2 and (Fig. 2C). As a final experiment, a high irradiation dose of 0.6 mW cm −2 was used to assess the inactivation potential within a much shorter time frame. Irradiation for 1 h resulted in a reduction of 0.4150 log 10 which reached 1.2943 log 10 reduction by 4 h and 2.309 log 10 (385 times) after 8 h in comparison to untreated controls samples at the corresponding times. (Fig. 2D,E). All experimental conditions demonstrated the stability of untreated SARS-CoV-2 which was left at room temperature in PBS, as shown by the marginal reduction of viral titer over time.
Influenza A virus is susceptible to 405 nm inactivation in the absence of photosensitizers. Given the observations derived from SARS-CoV-2, a separate inactivation study using a different lipidenveloped RNA virus was conducted by using influenza A Puerto Rico (A/H1N1/PR8-Mount Sinai) virus strain. Irradiation with a high dose of 0.6 mW cm −2 suggested a time dependent reduction of infectious titers as calculated by the 0.1619 log 10 , 0.5609 log 10 , and 1.6115 log 10 (66 times) reductions at 1, 2, 4 and 8 h respectively (Fig. 3A). And the reduction of plaques was apparent (Fig. 3B). www.nature.com/scientificreports/ The stability of IAV virus at room temperature for a period of 8 h was found to be the negligible in untreated IAV spiked PBS samples (Fig. 3A).    www.nature.com/scientificreports/ In this case however, a total reduction of 0.0969 (approximately 2 times) in comparison to the untreated after 8 h of irradiation was observed (Fig. 4A,B) indicating a lower rate of inactivation (despite identical dosing) in contrast to the lipid-enveloped RNA viruses tested in this study. The plaque reduction at 8 h did not indicate the same dramatic reduction as observed with the latter studies.

Encephalomyocarditis virus (EMCV) as a model non-enveloped virus indicates reduced sus
Disinfection potential of standard LED lighting containing spectral wavelengths from 400 to 420 nm. As shown in Fig. 1D, standard LED lighting (without the specific addition of 405 nm light) has a small but measurable amount of disinfecting light (0.006 mW cm −2 ) in the 400 nm to 420 nm range.
To quantify the disinfecting effect of this light, irradiations were performed for 4 h, 8 h, 12 h, and 24 h. A reduction of 0.7 Log was observed by 4 h in comparison to the controls. By 24 h, a total of 1.7 log was observed using a standard LED light (Fig. 5).

Discussion
The ongoing SARS-CoV-2 pandemic has affected day-to-day functions in the entire world, raising concerns not only with regards to therapeutics but also in the context of virus survivorship and decontamination 36 . Taking into consideration the rapid spread of SARS-CoV-2 from person to person by droplets, aerosols, and fomites, whole-room disinfection systems can be viewed as a supplement to best practices for interrupting transmission of the virus.
Given the ongoing COVID-19 pandemic, we wanted to explore the impact of 405 nm enriched visible light technology on inactivation of respiratory pathogens such as SARS-CoV-2 and influenza A virus.
Without the use of exogenous photosensitizers, we were able to show that irradiation with low intensity (0.035 mW cm −2 ) visible light yielded a reduction of log 10 0.3288 inactivation after 4 h (0.5 J cm −2 ) and a total log 10 1.0325 inactivation of SARS-CoV-2 after 24 h (3.02 J cm −2 ). A slightly higher dose (0.076 mW cm −2 ) resulted in log 10 1.5393 reduction after 24 h (6.56 J cm −2 ) while an irradiation dose of 0.150 mW cm −2 showed a reduction log 10 2.0056 after 24 h (12.96 J cm −2 ) of irradiation. Finally, increasing the dose to 0.6 mW cm −2 yielded log 10 2.3010 reduction only after 8 h (17.28 J cm −2 ), indicating a both time and dose dependent inactivation of infectious viruses.
The irradiations using standard LED lighting raise some interesting questions for further discussion. With nearly 6× the amount of disinfecting light as compared to traditional fluorescent lighting but 1/6th the lowest amount of 405 nm used in the study; it is conceivable that a reduction could be observed. The magnitude of this effect at less than 24 h was larger than expected based on other irradiations performed in this study (Fig. 5). These results at 24 h were largely consistent with other irradiation levels and reinforce the general observation of a 405 nm dose-dependent effect. The intermediate irradiation levels (4 h, 8 h, and 12 h) were generally larger than expected even when accounting for experimental uncertainty.
A similar effect was observed by Bache, et al. in whole-room bacterial reduction studies 37 . They saw a disinfecting effect for irradiance values as low as 0.0023 mW cm −2 . This effect was shown to be uncorrelated to the level of irradiance used suggesting that the mere abundance of 405 nm light can initiate the oxidizing chemical reaction. Nevertheless, our results clearly show a dose-dependent effect. One possible explanation for this observation is that the lower irradiance levels expose different responses by specific organisms within a population based on the individual biology of that organism. Clearly, these results suggest that additional factors may be at work and warrant further investigation.
We selected conventional plaque assays as the read out to specifically estimate infectious virus titers upon disinfection. Methods based in the quantification of viral RNA via PCR based techniques might be misleading as they detect viral RNA from both infectious and noninfectious virions.
SARS-CoV-2 is a lipid-enveloped virus composed of a ssRNA genome and our data indicates its susceptibility to visible light mediated inactivation. To further confirm these observations, we used influenza A virus. which is another human respiratory virus with a lipid envelope and a segmented-RNA genome. Upon irradiating for 1 h at 0.6 mW cm −2 (2.16 J cm −2 ), we observed a total reduction of log 10 0.1619 for the influenza A virus compared to the reduction of log 10 0.4150 for SARS-CoV-2 under the same conditions. While both viruses have lipid envelopes, there is clearly a difference here that will require further study. One possible explanation is the difference in the virion size creating a physically smaller cross-section for absorption. (IAV ~ 120 nm and SARS-CoV-2 ~ 200 nm) 38,39 . Nevertheless, both viruses were largely inactivated after 8 h achieving more 1.5 log 10 reduction. Intriguingly, it was observed that both RNA viruses were able to remain stable at room temperature for at least 24 h, indicating minimal decay which is consistent with previous studies 36,40 . We next irradiated a non-enveloped RNA virus, EMCV. Previous results for visible light against non-enveloped viruses demonstrated the need for external photosensitizers such as artificial saliva, blood, feces, etc 30,36 . Without a porphyrin containing medium, we expected little to no inactivation when this virus was irradiated with visible light. For these measurements, we used the highest available irradiance of 0.6 mW cm −2 . As anticipated, we observed only a log 10 0.0969 reduction after 8 h, however, this appears to be with the statistical precision of the measurement based on the results obtained from shorter irradiations (1, 2, and 4 h). For comparison, a study involving the M13-bacteriophage virus (a non-enveloped virus) showed a 3-Log reduction using an irradiance of 50 mW cm −2 (almost 100 times greater than the highest irradiance used in this study) for 10 h at 425 nm further supporting the idea that non-enveloped viruses may require higher doses of visible light 41 .
Our study was conducted using a neutral liquid media composed of PBS without any photosensitizers and we were able to show that visible light can indeed inactivate lipid-enveloped viruses, differing from the theory that states that photosensitizers are a requirement for inactivation. While these results provide insight into the basic science involved, they were performed within the context of the applied science needed to show the potential www.nature.com/scientificreports/ impact of this technology upon the current COVID-19 pandemic. By using safe, commercially practical irradiance levels, our results are more directly translatable to occupied rooms in the clinical environment.
Other studies which used visible light-based irradiation have shown similar results in the absence of photosensitizers, indicating the possibility of an alternative inactivation mechanism 23,25,30 . Studies have proposed two theories for this observation primarily due to non-405 nm wavelengths emitted by the source: (1) some amount of 420-430 nm emitted from the source is contributing to the viral inactivation 42 , and (2) the presence of UV-A (390 nm) within the source. This wavelength is known to create oxidative stress upon viral capsids 43 .
Longer wavelengths, such as 420-430 nm, have shown inactivation of the murine leukemia virus (MRV-A) 42 . While this is an intriguing study, it used a broad-spectrum lamp with optical filters to selectively identify the spectrum primarily responsible with their results. Unfortunately, they did not quantify the amount of light (using radiometric units) within the spectrum of interest used to irradiate the virus. While the transmission profile of the filters used was provided, it does not consider the spectral composition of the source itself making any direct quantitative comparison between our studies impossible. It is interesting to note that they did observe viral inactivation in their controls from wavelengths less than 420 nm confirming the qualitative findings of our study without confirming the specific use of 405 nm. This suggests that the viral inactivation is a likely a broad response (> 20 nm) with relative contributions unique to the chemistry of each organism. They also considered much longer exposures (~ 7 days) and much higher illuminance (> 200 lx) than that used in our study although this is again difficult to compare given the lack of radiometric quantification of their light source. It is important to note that the control samples used in our study were exposed to the same overhead (non-405 nm) lights as the irradiated samples and our results are the observed difference between the two demonstrating the contribution from 405 nm over and above that potentially from 420 to 430 nm. Future experiments can further quantify the potential effect.
The other theory, potential UV-A irradiation, was historically applied to lamp-based sources with broad spectral (> 100 nm) outputs. Again, the use of LED technology addresses this question as the peak irradiance at 390 nm of the device used in this study was < 1% of its peak irradiance at 405 nm without the need for any additional filtration. Future experiments can further quantify the potential effect.
Another consideration to be addressed is thermal heating of the virus by the LED source. Tsen and Achilefu used a pulsed laser method at 425 nm 44 with ~ 100 mW cm −2 average power density for < 2 h while simultaneously measuring the sample temperature with a thermocouple. They detected less than a 2 °C demonstrating minimal temperature impact even under a power density nearly 9 orders of magnitude larger than that used in this study. This was confirmed by our thermocouple measurements as stated earlier. Nazari et al. used an 805 nm source with an average power density of > 0.3 W cm −2 for 10 s, nearly 1000 times that used in this study 45 . While the total energy delivered was more comparable to that used in our study, they did not make explicit temperature measurements, their analysis ruled out any potential thermal effects.
One possible explanation for the observed differences between the enveloped and non-enveloped organisms is absorption of the 405 nm light by the lipid envelope itself. This could, in turn, lead to the creation of reactive oxygen species (causing an oxidative effect) or simply destruction of the envelope leading to a denaturing of the organism. This question could serve as the basis for a range of future studies.
The results obtained suggest that the performance of visible light against SARS-CoV-2 is similar to other organisms commonly found in the environment such as S. aureus. Previous studies have shown that the visible light irradiance levels used in this study (0.035 mW cm −2 to 0.6 mW cm −2 ) reduce bacteria levels in occupied rooms and improve outcomes for surgical procedures. It is therefore reasonable to conclude that visible light might be an effective disinfectant against SARS-CoV-2. More importantly, this disinfection can operate continuously as it is safe for humans based upon the exposure guidelines in IEC 62471 46 . This means that once it has been in use for a period of time, the environment will be cleaner and safer at all future times including when it is occupied by humans.
One limitation of this study is that the inactivation assays were performed in static liquid media as opposed to aerosolized droplets. While the use of visible light in air disinfection has been briefly studied where it was shown that its effectiveness increased approximately fourfold 47 , further studies involving dynamic aerosolization are needed to better understand the true potential of visible light mediated viral inactivation.
In any case, our study shows the increased susceptibility of enveloped respiratory viral pathogens to 405 nm mediated inactivation in the absence of photosensitizers. The irradiances used in this study are very low and might be easily applied to disinfect occupied areas safely and continuously within hospitals, schools, restaurants, offices and other locations. Of particular interest is the potential for standard LED lighting to play a role in reducing the presence of SARS-CoV-2 in the environment.

Conclusions
We have demonstrated the basic science of inactivation of enveloped viruses such as SARS-CoV-2 and Influenza-A using 405 nm visible light within the context of the applied science required for this technology to have an impact upon the current COVID-19 pandemic. Without the need for exogenous photosensitizers and by using safe, commercially practical irradiance levels, our results can be easily translated to the clinical environment.

Future efforts
Future work should focus on explaining the difference between the enveloped and non-enveloped results. This may include transmission electron microscopy (TEM), hemagglutination assay (HA), or other methods focusing on the potential role that a mediated reaction (due to the envelope itself) might play. The size of the virion particle may play a role in photoelectric absorption and could be studied for different viral species. We acknowledge that while unlikely, other wavelengths of visible light, beyond 400-420 nm may play a role in the inactivation process