A standardized extract of Asparagus officinalis stem improves HSP70-mediated redox balance and cell functions in bovine cumulus-granulosa cells

Heat shock (HS) protein 70 (HSP70), a well-known HS-induced protein, acts as an intracellular chaperone to protect cells against stress conditions. Although HS induces HSP70 expression to confer stress resistance to cells, HS causes cell toxicity by increasing reactive oxygen species (ROS) levels. Recently, a standardized extract of Asparagus officinalis stem (EAS), produced from the byproduct of asparagus, has been shown to induce HSP70 expression without HS and regulate cellular redox balance in pheochromocytoma cells. However, the effects of EAS on reproductive cell function remain unknown. Here, we investigated the effect of EAS on HSP70 induction and oxidative redox balance in cultured bovine cumulus-granulosa (CG) cells. EAS significantly increased HSP70 expression; however, no effect was observed on HSP27 and HSP90 under non-HS conditions. EAS decreased ROS generation and DNA damage and increased glutathione (GSH) synthesis under both non-HS and HS conditions. Moreover, EAS synergistically increased HSP70 and HSF1 expression and increased progesterone levels in CG cells. Treatment with an HSP70 inhibitor significantly decreased GSH level, increased ROS level, and decreased HSF1, Nrf2, and Keap1 expression in the presence of EAS. Furthermore, EAS significantly increased progesterone synthesis. Thus, EAS improves HSP70-mediated redox balance and cell function in bovine CG cells.


Effect of EAS on the specific induction of HSP70.
To determine the concentration of EAS required to induce the expression of HSP27, HSP70, and HSP90 in bovine CG cells, we examined various concentrations of EAS (0.5, 1, and 5 mg/mL). A significant increase (P < 0.05) in HSP70 expression was observed after treatment with 5 mg/mL EAS (Fig. 1a). However, the expression of HSP90 and HSP27 was not influenced by EAS (Fig. 1b,c). Since HSPs are induced by HS, we evaluated the effect of EAS on HSP expression after 6 h of HS (41 °C) treatment in comparison with that under non-HS conditions in bovine CG cells. Similar to the findings shown in Fig. 1, EAS treatment specifically increased HSP70 expression under non-HS control conditions (Fig. 2a). Moreover, in these experiments, the expression of HSP90 (Fig. 2b) and HSP27 (Fig. 2c) did not change.
HS induced an increase in HSP70 and HSP90 expression. Interestingly, HSP70 expression was synergistically increased by HS and EAS (Fig. 2). Similar to the induction of HSP70, HSP70 protein levels were significantly increased (P < 0.05) after treatment with 5 mg/mL EAS and synergistically increased (P < 0.01) by HS and EAS (Fig. 3a,b). The immunodetection of HSP70 also showed a similar increase in HSP70 gene expression (Fig. 3c,d).
HSF1 is a transcription factor that induces HSP70 expression by HS 23 . As shown in Fig. 4a, HSF1 expression was significantly increased by HS and HS + EAS, whereas no effect of HS and HS + EAS on HSF2 expression was observed (Fig. 4b).
Effect of EAS on ROS generation and DNA damage. After HSP70 expression was enhanced by EAS treatment in bovine CG cells, we investigated the effect of EAS on HSP70 expression and oxidative and redox balance 24 . As shown in Fig. 5a and c, EAS treatment significantly reduced ROS levels under both non-HS and HS conditions. ROS are known to cause apoptosis associated with mitochondrial dysfunction and single and double-stranded DNA breaks 25 . Therefore, we hypothesized that EAS could reduce DNA damage caused by HS-induced ROS generation. The levels of γH2AX were significantly increased by HS. However, these levels were significantly decreased by combining HS and EAS (P < 0.05, Fig. 5b,d).
Effect of EAS on GSH synthesis. GSH was detected more strongly in the nuclei than in the cytoplasm (Fig. 6a). EAS treatment significantly increased GSH levels (P < 0.05) in non-HS cells (Fig. 6a,b).

Effect of EAS on expression of genes related to oxidative stress and redox balance.
To determine whether EAS protects against DNA damage by maintaining the redox status, we focused on genes related to oxidative stress and redox balance. The expression of glutamate cysteine ligase (GCL) and glutathione synthetase (GS),, which are involved in GSH synthesis in mammalian cells 26 , was significantly increased by EAS treatment under non-HS conditions (Fig. 7a,b). Since GSH synthetic pathways, including GCL and GS activation, are dependent on nuclear factor erythroid 2-related factor2 (Nrf2) regulation 27 , we analyzed the expression of Nrf2 and Kelch-like ECH-associated protein 1 (Keap1). The expression of both Nrf2 and Keap1 was significantly increased by HS treatment (Fig. 7c,d). The Nrf2 levels in the HS and HS + EAS (5 mg/mL) groups were significantly higher than those in the control group (P < 0.01). In contrast, the HS + EAS (5 mg/mL) group had a lower Keap1 mRNA expression than the control, EAS (5 mg/mL), and HS groups (P < 0.01) (Fig. 7d). www.nature.com/scientificreports/ EAS is extracted from Asparagus officinalis L., which contains high levels of antioxidants, such as carotenoids, steroidal saponins, and flavonoids, and antioxidant activity has been demonstrated in other extracts of this vegetable 28 . Therefore, we analyzed the expression of antioxidant enzymes, including superoxide dismutase (SOD)1, SOD2, peroxiredoxin (PRDX)2 and PRDX6, to evaluate the antioxidant effect of EAS under non-HS conditions. The expression of SOD1 and SOD2 was significantly increased by HS treatment (P < 0.05) (Fig. 7e,f). Although no effect of EAS was observed on SOD1 and SOD2 expression under non-HS conditions, EAS treatment significantly increased the expression of both SOD1 and SOD2 under HS conditions. Similar to the expression patterns of SOD1 and SOD2, EAS treatment significantly increased the expression of PRDX2 and PRDX6 (P < 0.05) (Fig. 7g,h). www.nature.com/scientificreports/ plays a role in the balance of the cellular redox status in bovine CG cells. We hypothesized that HSP70 induction by EAS triggers the regulation of the balance between ROS and GSH levels, which is required for maintaining the cellular redox status in bovine CG cells. To test this hypothesis, cells were treated with EAS (5 mg/mL) and 10 µM pifithrin-µ (PES). PES has been reported to inhibit HSP70 by interacting with the C-terminal peptide substrate-binding domain 29 . The viability of cells treated with 10 µM PES was 77% (Supplemental Fig. 1). ROS levels decreased following EAS + PES treatment (Fig. 8a,c). In contrast, the levels of GSH that had been increased by EAS treatment were significantly decreased (Fig. 8b,d). Moreover, the expression of HSF1 and Nrf2 increased by EAS treatment was significantly decreased by the inhibition of HSP70 (Fig. 9a,b). In addition, Keap1 expression was significantly decreased by the inhibition of HSP70 (Fig. 9c).

Effect of HSP70 inhibition on the redox status of bovine CG cells treated with EAS.
Effect of EAS on progesterone synthesis. Bovine CG cells not only play a role in oocyte development but also perform an endocrine role of steroid hormone secretion 30   www.nature.com/scientificreports/ regulating pregnancy and conceptus growth in cattle 31 . We measured P4 levels to further investigate the effect of EAS treatment on steroidogenesis and found that P4 levels were significantly increased by EAS supplementation compared with that in the control group (P < 0.05) (Fig. 10).

Discussion
EAS has been shown to increase the expression of HSP70 mRNA and protein in HeLa cells, neuronal cell NG108-15, and hepatocyte cells [32][33][34] . Moreover, HSP70 overexpression increased the GSH/GSH disulfide ratio while reducing ROS levels under hypoxia and glucose deprivation conditions 18 . Therefore, the current study investigated whether EAS treatment increases HSP70 expression. GSH is a tripeptide of gamma-glutamylcysteinylglycine with antioxidant properties, whose synthesis reduces HS-induced ROS generation with DNA damage to maintain the redox status in bovine CG cells.
In the present study, EAS at a concentration of 5 mg/mL significantly increased HSP70 mRNA and protein levels in bovine CG cells following 6 h of incubation. This result is similar to that of a previous study, in which hepatocyte cells were treated with 4 mg/mL EAS during incubation for 4 h 34 . In addition, HeLa cells showed increased HSP70 mRNA and protein expression when treated with a lower dose of EAS (4 mg/mL) but after a longer incubation time (24 h) than that in the current study (6 h) 32 . The variation in the effect of EAS dose in cells may be because of differences in cell type and culture conditions. HSP70 expression-enhancing activity by EAS may arise from asparagine, which has been found to elevate HSP70 expression in human monocyte HL-60 cells 20 . On the other hand, resistance to anti-cancer drugs due to the overexpression of HSP27 and HSP90 has been reported in several studies, but the expression of HSP27 and HSP90 in bovine CG cells was not affected by EAS treatment 35 . Therefore, our results confirm the unique effect of EAS on HSP70 induction in bovine CG cells under non-HS conditions.   37 . In this study, HSP70 induction by EAS occurred under both non-HS and HS conditions, and it was highest under HS conditions 35 . These data suggest that the combination of EAS and HS synergistically affects HSP70 induction in bovine CG cells. The induction of HSF1, which is one of the main transcription factors of HSP70, by EAS has been reported in neuronal cells NG108-15 34 . The significant increase in HSF1 gene expression by EAS in the present study supports the involvement of EAS in the HSP70 inducing pathway in bovine CG cells 37 . Under cellular stress, HSF1 is activated and releases HSP70 to prevent the formation of misfolded polypeptides 38 . In this study, HSF1 gene expression was induced by EAS supplementation in both non-HS and HS conditions, and it was highest with EAS supplementation under HS conditions. These data suggest that EAS and HS have synergistic effects on the induction of HSF1 in bovine CG cells. HSF2 is a HS transcription factor co-expressed with HSF1, which is activated in response to distinct developmental cues or differentiation stimuli 38 . However, HSF2 was not affected by EAS in the current study and supplementation with EAS led to increased HSP70 expression under non-HS conditions. However, HSF1 expression was only induced   34 . Hence, it is conceivable that an increase in HSP70 expression by EAS is induced as a result of the asparagine content and enhancement of HSF1, with EAS exerting synergistic effects with HS on HSP70 induction. Several studies have indicated that HSP70 has apoptosis-suppressive effects and anti-inflammatory activity, indicating that HSP70 exerts a cytoprotective function against various stresses 39 . In addition, the overexpression of HSP70 reduces ROS induced by hypoxia and glucose deprivation 18 . These results indicate that EAS with www.nature.com/scientificreports/ a unique inducible HSP70 could protect cells against ROS under various stress conditions. Moreover, EAS was found to significantly reduce the amyloid beta peptide-induced production of ROS in differentiated rat PC12 cells 40 . In this study, EAS treatment significantly inhibited ROS expression in both normal and HS conditions in bovine CG cells by activating GSH generation and antioxidant enzymes, such as SOD and PRDX. Moreover, ROS activates c-Jun N-terminal kinase protein, which also increases under HS conditions 41 . EAS clearly reduced the c-Jun N-terminal kinase protein induced by hydrogen peroxide in fibroblast cells 42

. In conclusion, EAS reduced HS-related ROS generation in bovine CG cells, which can be explained by the upregulation of GSH generation together with antioxidant enzymes and the downregulation of c-Jun N-terminal kinase protein.
ROS-induced damage can cause both single-and double-stranded DNA breaks 25 . In this study, EAS significantly reduced γH2AX levels under HS conditions. ROS generation from normal cellular metabolism and HS induces DNA damage in cells 43 . In a previous study, EAS was shown to reduce cell damage induced by nitric oxide donor sodium nitroprusside or the hypoxia mimic reagent cobalt chloride of NG108-15 cells 33 . HSP70 has the ability to repair DNA damage caused by HS 25 . In this study, HSP70 induction by the synergistic effect of EAS and HS contributed to the reduction of DNA damage in bovine CG cells supplemented with EAS under HS conditions. Moreover, GSH also contributes to DNA repair activity, and the expression of GSH in the nucleus enhances protection against DNA damage 44 . In the present study, GSH generation was increased by EAS in bovine CG cells under non-stress and HS conditions. These results indicate that EAS reduced DNA damage under non-HS conditions and was within the acceptable range of non-toxic levels of ROS production due to the enhancement of HSP70 and GSH levels.
In the present study, EAS increased GSH in both non-HS and HS conditions, but only cells supplemented with EAS under non-HS conditions were significantly different when compared with the control group. In agreement with this observation, previous reports have suggested that overexpression of HSP70 enhances GSH expression 18 . However, the GSH levels in the control group were similar to those in the HS group, which were exposed for 6 h at 41 °C. In HeLa cells, HS treatment for 1, 2, or 3 h at 42 °C resulted in the highest increase in GSH level at 1 h and the lowest increase at 3 h 27 . GSH biosynthesis is required by the action of two ATP-dependent enzymes: GCL, which assembles the formation of c-glutamyl-cysteine from glutamate-cysteine, and GS, which is involved in the ligation of c-glutamyl-cysteine to glycine in another ATP-dependent reaction to yield GSH 26 . Our results showed that EAS increased the expression of GCL and GS under non-HS conditions. The transcription factor Nrf2, which has the potential to induce GCL and GS, is activated by ROS production 45 . Moreover, HSF1 may induce Nrf2 by activating p62, which can separate Nrf2 from Keap1 46 . In this study, GSH levels were not decreased by HS because of the compensatory effect of HSF1 with Nrf2, which can influence GSH levels after 6 h of HS treatment. Therefore, there is a need to study the effect of HS treatment on GSH synthesis and the induction of genes. A previous study showed that reduced Keap1 expression is logically related to the induction of Nrf2 47 . Together with the induction of Nrf2, EAS supplementation reduced Keap1 and induced HSF1 expression in this study. EAS treatment increased Nrf2 protein levels in NG108-15 neuronal cells 33 . In conclusion, EAS increased GSH expression and γ-glutamyl cycle mRNA expression due to Nrf2, which was induced by the compensation effect of HSF1 with Nrf2 and the antioxidant content of EAS.
The higher expression of antioxidant enzyme genes in the HS group in the present study was similar to that reported in a previous study, in which HS induced SOD and PRDX expression in pig skeletal muscle 48 and HeLa cells 27 , respectively. In another study, HS was reported to upregulate HSP70 and SOD2 in Chinese hamster lung fibroblast V79 cells 41 . Interestingly, the HSP family and antioxidant system reduced the harmful effects of ROS 49 . In this study, EAS increased HSP70 expression, antioxidant enzymes, and reduced ROS levels. Nrf2 expression has been shown to induce PRDX activity in HeLa cells 26 and SOD activity in mesenchymal stem cells 50 . In this study, Nrf2 expression was highest in bovine CG cells treated with EAS under HS conditions, indicating that the induction of PRDX and SOD was due to Nrf2 expression. EAS extracted from asparagus (Asparagus officinalis L.) contains high levels of antioxidants, including carotenoids, steroidal saponins, and flavonoids 28 . Therefore, the www.nature.com/scientificreports/ enhanced antioxidant activity of EAS treatment results from the induction of Nrf2 expression and the antioxidant content of asparagus (Asparagus officinalis L.). The superoxide dismutase-like activity of Trapa japonica shell extract was lower than that of ascorbic acid 51 . In addition, the 1,1-diphenyl-2-picryl hydrazyl radical scavenging activity of saffron petals, a by-product of saffron, was also significantly lower than that of ascorbic acid 52 . Thus, the improvement of antioxidant activity by EAS supplementation is not caused by the antioxidant components contained in EAS, but by EAS itself.
PES has been shown to inhibit HSP70 29 . In this study, PES reversed the effect of EAS on GSH and ROS levels, indicating that HSP70 induction by EAS regulated the levels of GSH and ROS in bovine CG cells. A previous study reported a correlation between the transcription factors Nrf2 and HSF1 for the protection of cells 53 . There is evidence that Nrf2 and HSF1 compensate each other; the induction of HSP70 by methionine deprivation is dependent on Nrf2 but independent of HSF1 54 . Both Nrf2 and HSF1 play important roles in cellular redox processes because of their ability to influence the levels of HSP70 and GSH. Therefore, distinct cell survival pathways, such as the HS response and Keap1/Nrf2/ARE pathway, are regulated by Nrf2 and HSF1 55 . In the present study, PES also reversed the induction effect of both Nrf2 and HSF1. PES reduces the nuclear translocation of the nuclear factor-κB (NF-κB) pathway, which regulates the transcription of various gene families including: stress response, apoptosis, and receptor genes, and influences cell survival, differentiation, and proliferation 56 . Nuclear translocation of NF-κB p65 enhances the ability of Nrf2 and plays a role in the antioxidant response in human kidney-2 cells 57 . In addition, HSF1 activation in intestinal epithelial cells during HS is regulated by the NF-κB pathway 58 . Moreover, PES reduced the levels of Keap1, which combined with Nrf2 to regulate the antioxidative protection system 55 . These results indicate that HSP70 induction by EAS improved the redox balance by regulating the ROS and GSH levels through HSF1, and Nrf2/Keap1 pathway in bovine CG cells.
The beneficial effect of EAS on P4 synthesis demonstrated in the study was similar to that reported in a previous study, in which the oral administration of asparagus root extract enhanced P4 levels in rats 59 . This indicates that bovine CG cells are important for reproductive functions, especially steroidogenesis. These findings indicate that EAS contributes to the improvement of P4 synthesis in bovine CG cells.
In conclusion, EAS was shown to induce HSP70 under non-HS conditions, exerting a synergistic effect with HS on HSP70 induction in bovine CG cells. Furthermore, EAS had beneficial effects, reducing the DNA damage induced by ROS, and increasing GSH synthesis and antioxidant enzyme levels to maintain the redox status, in addition to the P4 levels in bovine CG cells. HSP70 induced by EAS regulated the Nrf2/Keap1 pathway and HSF1 transcription factor levels, which contributed to the ROS and GSH levels in bovine CG cells.
The findings presented in this study collectively demonstrate that EAS has potential uses in the regulation of reproductive functions by reducing physical stress and improving the properties of reproductive cells.

Methods
EAS, which is produced from asparagus (A. officinalis L.) grown in Hokkaido, was provided by Amino Up Co., Ltd. (Sapporo, Japan). EAS was manufactured according to a previously described method 60 .
Bovine CG cell culture. Bovine ovaries were collected from a local abattoir and transported to the laboratory at 20 °C. The ovaries were washed several times with sterile saline solution. Follicular contents were aspirated from follicles (2-8 mm in diameter), which lacked evident signs of atresia using a disposable 18-gauge needle attached to a 10-ml syringe. These follicular contents were released into a sterile plastic Petri dish, and cumulus-oocyte complexes were picked up. After picking up the cumulus-oocyte complexes, the suspension of follicular fluid with floating CG cells was cultured in 5% fetal bovine serum (FBS) in Dulbecco's modified Eagle's medium (high glucose) (DMEM) (Wako, Osaka, Japan) supplemented with 0.06 g/l penicillin G potassium (Nacalai Tesque, Kyoto, Japan) and 0.1 g/L streptomycin sulfate (Nacalai Tesque) at 38.5 °C under 5% CO 2 in air. After overnight incubation, a network of theca cell clusters was gently peeled from the culture dish surface. Bovine CG cells remaining in the culture dish were washed with calcium-and magnesium-free phosphate-buffered saline (PBS) (-) and cultured in 5% FBS in DMEM at 38.5 °C under 5% CO 2 in air. After reaching confluency, bovine CG cells were washed with PBS (-) and dissociated from the substratum with PBS (-) containing 0.05% trypsin and 0.53 mM EDTA for 2 min at 38.5 °C in a CO 2 incubator. After supplementation with 5% FBS in DMEM to inhibit trypsin activity, the cell suspension was centrifuged at 1,200 × g for 3 min.
Viable cells were plated at a density of 1.0 × 10 5 cells/mL onto 4-well culture plates (Thermo Fisher Scientific) or 8-well slides and chambers (SPL Life Sciences Co., Ltd, Pocheon, Korea) and cultured at 38.5 °C under 5% CO 2 in air. After cells became 70% confluent, the medium was replaced with 0.9 ml of 5% FBS in DMEM together with 0.1 ml of EAS stock solution in PBS (-), and 0.1 ml of PBS (-) was added in control group. The cells were then cultured at 38.5 °C or at 41 °C under 5% CO 2 in air under HS conditions. Experimental design. Cells were cultured at 38.5 °C for 6 h with 0.5, 1, and 5 mg/mL of EAS for mRNA expression of HSP27, HSP90 and, HSP70.To compare the effect of EAS under non-HS and HS conditions, cells were cultured at 38.5 °C and 41 °C for 6 h with 5 mg/mL of EAS for western blotting, immunostaining, fluorescent staining and mRNA expression.
To inhibit HSP70 activity, cells with 5 mg/mL of EAS supplementation were treated with or without 10 µM PES under non-HS conditions for 12 h.
To determine the optimal concentration of PES, cells were treated with 5 µM, 10 µM, or 20 µM PES under non-HS conditions for 12 h and cell viability was analyzed using the Live-Dead Cell Staining Kit (ALX-850-249, Enzo Life Sciences AG, Lausen, TX, USA) according to the manufacturer's instructions.
To determine the influence of EAS on P4 synthesis, CG cells were cultured at 38.5 °C for 12 h with 5 mg/ mL of EAS. The primary and secondary antibodies were diluted with an immunoreaction enhancer, Can Get Signal (Toyobo). Membranes were washed with TBS-T before detection of bound antibodies using the WSE-7120EzWestLumi plus (Atto Corporation, Tokyo, Japan). Chemiluminescent signals were captured using LumiCube (Liponics, Inc., Tokyo, Japan). The intensity of the bands was analyzed using ImageJ software v1.52A (National Institutes of Health; http:// imagej. nih. gov/ ij/). Fluorescence images were acquired using an EVOS™ M5000 imaging system (Thermo Fisher Scientific Inc.). Fluorescence intensity was quantified using ImageJ software v1.52A (National Institutes of Health; http:// imagej. nih. gov/ ij/). For corrected total cell fluorescence (CTCF), we used the following formulas 61 :

Immunostaining for HSP70 and γH2AX.
1. CTCF = integrated density (total area of selected cells × mean fluorescence of background readings) 2. CTCF per cell = CTCF/ Ncells where "Integrated Density" is the integrated intensity of the pixels for all cells in the image, total cell area is the number of pixels of all of the cells, background fluorescence is the average mean gray value of nearby regions containing no cells, and Ncells is the number of cells that was measured by counting fluorescently labeled nuclei from images.

Measurement of progesterone.
The culture medium was collected and centrifuged. The supernatant of the culture medium was used to measure progesterone through enzyme-linked immunosorbent assay using commercial kits following manufacturer's instructions (ADI-900-011, Enzo Life Sciences, Inc., USA).