Future intensification of extreme Aleutian low events and their climate impacts

Extreme Aleutian Low (AL) events have been associated with major ecosystem reorganisations and unusual weather patterns in the Pacific region, with serious socio-economic consequences. Yet, their future evolution and impacts on atmosphere–ocean interactions remain uncertain. Here, a large ensemble of historical and future runs from the Community Earth System Model is used to investigate the evolution of AL extremes. The frequency and persistence of AL extremes are quantified and their connection with climatic variables is examined. AL extremes become more frequent and persistent under the RCP8.5 scenario, associated with changes in precipitation and air temperature patterns over North America. Future changes in AL extremes also increase the variability of the sea surface temperature and net heat fluxes in the Kuroshio Extension, the most significant heat and energy flux region of the basin. The increased frequency and persistence of future AL extremes may potentially cause substantial changes in fisheries and ecosystems of the entire Pacific region as a knock-on effect.

www.nature.com/scientificreports/ the consequences of a warming scenario on the North Pacific mean state, the AL variability, and the El Niño teleconnections 26,34,35 . However, the future frequency of extreme AL events and their oceanic and atmospheric response still remain unclear. Here, we assess changes in AL extreme events by comparing the intensity and frequency of North Pacific extreme SLP patterns in past and future simulations of the Large Ensemble of the Community Earth System Model version 1 (CESM1-LENS 36 ). We show that extreme AL events become stronger and more frequent under the RCP8.5 scenario. To consider wider impacts due to future changes of the AL, its relationship with precipitation and SAT over North America, and the SST and net heat flux over the North Pacific is examined. We quantify the oceanic and atmospheric response that follows the atmospheric extremes by evaluating the change in the dominant period of common variability of the AL SLP and each one of these climate parameters.

Results
Increased persistence and frequency of future Aleutian Low extreme events. To quantify the persistence and frequency of extreme AL events, we use dynamical indicators to describe the dynamical state of the system (see "Methods"). Specifically, the inverse persistence indicates how a daily SLP pattern persists through time, whereas the instantaneous dimension represents the predictability and repeatability of that pattern throughout the time-series. The two dynamical indicators calculated for the future simulation of one example ensemble member (CESM1-LENS member 16) are used here for illustration purposes and are displayed in Fig. 1a. The two most extreme areas of the scatterplot (two red shaded upper (0.98) and lower (0.02) quantiles in Fig. 1a) represent the North Pacific daily SLP configurations that present extremely high and extremely low estimations for both dynamical indicators. The average of the points within the quantile of the extreme high dynamical properties, which represents conditions of low stability and predictability, displays a transitional North Pacific blocking pattern (Fig. 1b for the example member). This is consistent with the results found by Faranda et al. 37 for the North Atlantic, where blocking patterns were also associated with low persistence and high predictability. On the other hand, the extreme low quantile represents a deepened AL pattern (Fig. 1c for  www.nature.com/scientificreports/ the example member) and signifies increasing frequency and persistence of the pattern in the region. The deepening of the AL, in terms of magnitude, is also apparent when comparing the frequency distributions as well as the lowest 2% percentiles of the spatially averaged monthly AL SLP time-series of the historical simulations and the future RCP8.5 runs ( Supplementary Information, Fig. S1). Furthermore, an increase of points with extremely low dynamical properties in the future runs compared to the past is shown in 92% (33 out of 36) of the total of the ensemble simulations (Fig. 1d). The number of points (i.e. days of North Pacific SLP) falling within this extreme high quantile decreases in the future (Fig. 1e) in approximately 70% (25 out of 36) of the ensemble members. The dynamical indicators for the historical and RCP8.5 ensemble runs of the CESM1-LENS are presented in Fig. 2 (whole distribution, rather than just 2nd and 98th percentiles). The extension of both the upper and lower tails of the distribution of the inverse persistence indicates that future extreme North Pacific SLP patterns have an increased variability of their residence time in the area (Fig. 2a). A lower inverse persistence means that the dynamical system trajectory is slow leaving the neighborhood confined by one point 39 , and describe more stable dynamic fields that tend to have slower variations 40 . This means that when an SLP pattern emerges, it is more likely to persist in the region for a longer period in the future projections compared to the past simulations. The slight extension of the lower tail of the histogram in Fig. 2a suggests that the persistence of the stable SLP configurations governing the North Pacific (i.e. Aleutian Low and North Pacific High) increase under the RCP8.5 scenario. Similarly, extremely unstable SLP patterns, that are represented by trajectories rapidly leaving the neighborhood around one point, are equivalently increasing in the RCP8.5 scenario. The elongated upper tail of the inverse persistence histogram (Fig. 2a) indicates that these unstable SLP patterns in the area (i.e. spring transition pattern and North Pacific blocking pattern) will become more transient and more likely under global warming. On the other hand, a substantial shift occurs in the instantaneous dimension of the whole North Pacific SLP system (Fig. 2b). A low instantaneous dimension of a given atmospheric pattern suggests a higher likelihood for the pattern to emerge again in the system 37 , which describes the rarity of the daily SLP configuration examined in each time-step 40 . In other words, a lowering of the dimension indicates that the most unstable patterns will not be favored in the future climate and that the atmospheric circulation in this area will be more predictable. The lowering in dimension found here suggests that the dominant SLP patterns (i.e. a wintertime Aleutian Low and a summertime North Pacific High) occurring in the North Pacific will be more frequent in the future compared to unstable transitional patterns (i.e. the North Pacific blocking and the spring transition patterns). These results are pointing towards an increasing stability of atmospheric motions that are coherent with www.nature.com/scientificreports/ those found for the Atlantic basin 41 . The distribution differences in both indicators were tested with the two-sided Kolmogorov-Smirnoff test, suggesting significant distribution differences in each case (significance level of 99%).

Relationship of the intensified Aleutian Low with weather patterns over North America. Due
to its controls on atmospheric circulation, changes in the AL have the potential to impact weather patterns over North America. Here we examine the possible effects on surface air temperature (SAT) and precipitation. Regions where changes in the AL SLP significantly affect SAT over North America are presented in Fig. 3a. Atmospheric circulation patterns (e.g. anomalous winds) related to a deepened AL have been linked to warming trends over Canada and Alaska 42,43 . A negative correlation between the AL SLP and the SAT is predominant in most of the northern part of North America, which is stronger in the northwest. Contrastingly, southeastern North America is shown to be slightly positively correlated to the AL SLP. The coherence (see "Methods") between past and future simulations of the AL SLP and SAT is estimated in order to assess the change in the relationship between the fields at different periods. The frequency distribution of the global power of the wavelet coherence (i.e. distribution over frequencies averaged in time) between negatively correlated areas of AL SLP and SAT presents a shift towards higher power in the future members over all the periods considered ( Fig. 3b) (Kolmogorov-Smirnoff tests, 99% significance level; Supplementary Information, Figure S2). The intensified and more frequent extreme AL strengthens basin-scale winds resulting in warmer SAT  (Fig. 3c), emphasizing the influence of internal variability of the system, since the simulations are constrained by the same historical and RCP8.5 radiative forcing 44 . Still, the average global power of the wavelet coherence between AL SLP and SAT over North America is higher in the future compared to the past, during the periods between 4 and 40 months (Fig. 3c). This shift towards higher average global power suggests the enhanced influence of the future AL on the North American SAT (negatively correlated areas in Fig. 3a).
The differences between the frequency distributions of the global power of the wavelet coherence in the past and future simulations, as well as their average global power, were both significant (Kolmogorov-Smirnoff test, 99% significance level; Supplementary Information, Figure S2), however only minor discrepancies are noticeable between them (Fig. 3d,e). Although possible teleconnections with the AL may play some role, other mechanisms (e.g. the influence of ENSO and North Atlantic Oscillation) may be more important in driving the SAT variability over southeast North America 46 .
A deepened AL has been related to precipitation over North Pacific 3 and to the precipitation dipole over the U.S. west coast 5 . Specifically in California, an intensified AL increases the precipitation extremes through strengthened atmospheric vapor and enhanced atmospheric rivers 47 . The correlation between the AL SLP and precipitation over North America is presented in Fig. 4a, where precipitation in the northwest and southeast have a negative relationship with the AL SLP and the opposite stands for areas in continental US and Canada. The frequency distribution of the global power of the wavelet coherence between negatively correlated areas www.nature.com/scientificreports/ of AL SLP and precipitation presents a shift towards higher power in the future members over all the periods considered (Fig. 4b). The global power of individual past and future ensemble members follows similar patterns and present increasing global power in lower frequencies indicating higher common variability of the two fields on interannual temporal scales (Fig. 4c). Furthermore, the multi-ensemble average global power for the past and future members between AL SLP and precipitation over North America highlights an intensified relationship for periods greater than 10 months (Fig. 4c). Contrary to the results for the negatively correlated areas, the frequency distribution of the global power between the positively correlated areas of AL SLP and precipitation presents a shift towards lower power in the future simulations (Fig. 4d,e). Two-sided Kolmogorov-Smirnoff tests presented consistent distribution differences in each (positive and negative correlation) case (significance level of 99%; Supplementary Information, Figure S3). The evolution of the AL and precipitation and SAT over North America over different time scales in the future has also been examined through the calculation of their common variability during a near-future (2005-2050) and a far-future (2051-2100) period under the RCP8.5 scenario (Supplementary Information, Figure S5 and Figure S6). Breaking down the global power into separate future periods highlights that the future intensification of the relationship between the AL SLP and North American precipitation and SAT occurs throughout the whole time period (as opposed to only towards the end of the century).
Increased sea surface temperature and net heat flux in the Kuroshio Extension due to an intensified AL. The common variability of past and future simulations between the AL SLP and both net heat fluxes and SST in the Kuroshio Extension is analyzed in order to examine potential changes in their relationship at multiple frequencies. The global power of wavelet coherence of the AL SLP and the examined parameters is higher in the future members in all frequency bands (Fig. 5). It has been previously shown that the AL SLP affects the Kuroshio Extension jet and SST, as well as the variability of heat fluxes in the area 15,19,48 . Our results present the evolution of this relationship in time and highlight its intensification under the RCP8.5 future www.nature.com/scientificreports/ scenario. The intensified and more frequent extreme AL induce increased forcing to the Kuroshio Extension net heat flux and SST.
Although the global wavelet power patterns of the multiple ensemble members differ substantially from each other under the same scenarios (Fig. 5), on average they peak at the same frequencies (black dotted and dashed lines in Fig. 5a,b). The similar patterns on longer than annual scales indicate that the consistent mechanistic linkage between the AL SLP and the Kuroshio Extension net heat flux and SST is not altered by the internal variability of the system or the extreme variations of the individual fields (e.g. AL SLP extreme events). However, in periods shorter than the annual time scales (< 10 months) the individual ensemble members present a highly variable global power, showing that the internal variability of the system plays an important role in shaping the intra-annual relationships between the AL and SST and net hear flux. Periods larger than 64 months are not considered, due to the influence of the edge effects of the wavelet transform that become apparent in the low frequency bands. The evolution of the common variability between the AL and SST and net heat flux in the Kuroshio Extension at different time scales in the future under the RCP8.5 scenario has also been examined ( Supplementary Information, Figure S7). The comparison between historical simulations, the RCP8.5 runs during 2005-2050 and the RCP8.5 for the period 2051-2100 shows that the AL and SST increase in common variability happens mostly in the far-future (2051-2100). On the other hand, the near and far future periods are equally important for the increase in common variability between the AL SLP and the Kuroshio Extension heat flux.

Discussion and conclusions
A coupled climate model that includes physical, biogeochemical and ecosystem components was used to explore the intensification of North Pacific atmospheric extremes and their impact on atmospheric and oceanic parameters under climate change. We compared the historical runs and the 'business-as-usual' RCP8.5 scenario simulations of the Community Earth System Model-Large Ensemble. This analysis reveals an intensification of the North Pacific SLP under increased anthropogenic forcing, expressed as a future increase in frequency and persistence of AL extreme events.
The overall deepening of the North Pacific SLP in the future CESM1-LENS simulations and the increase of low SLP extreme events indicate that the AL pattern is strengthened under anthropogenic warming. AL variability is primarily linked to the North Pacific decadal climate variability 23,24 , which is a major source of uncertainty in the near-future model SLP projections 49 . Observational and modelling studies have demonstrated that internal variability alone can generate ENSO-like responses in the North Pacific atmospheric system 50 . Furthermore, remote connections with ENSO indirectly contribute to the AL variability via the atmospheric bridge 25 . ENSO events drive the AL SLP through nonlinear extra-tropical teleconnections triggered by the increased tropical SST anomalies 25,26 . Anomalously warm SSTs in the eastern tropical Pacific induce increased rainfall and heat from enhanced atmospheric convection. This results in upper-tropospheric divergence and vorticity which excite stationary Rossby wave trains moving poleward across the North Pacific resulting in a deepened AL 51 . Specifically, changes in the location and magnitude of ENSO SST anomalies in the equatorial Pacific alter the strength, position and persistence of the AL 34,52 . Gan et al. 34 also suggested a deepening of the AL in the twenty-first century, stressing the importance of the effects of tropical SST anomalies. As the AL drivers (i.e. North Pacific internal dynamics and teleconnections to ENSO) are shown to be highly linked to anthropogenic greenhouse gas forcing 52,53 , any associated change can potentially lead to enhanced AL extreme events. The deepening of the AL SLP projected by the future simulations shown here is consistent with the intensified and more frequent ENSO events predicted under increased anthropogenic forcing [30][31][32]35 . It is interesting to note that, unlike the CMIP5 inter-model comparison conducted by Gan et al. 34 associating climate model differences with natural variability, here we show that the internal variability combined with the signal of the radiative forcing alone can generate extreme changes in North Pacific SLP patterns under the RCP8.5 future emission scenario.
The changes detected in the dynamical indicators of the North Pacific SLP suggest that the persistence and frequency of SLP patterns will vary in the future. Specifically, the semi-permanent SLP patterns (i.e. Aleutian Low and North Pacific High) will become more persistent and more frequent, whereas the transitional patterns (i.e. North Pacific blocking and spring transition patterns) will become less stable and less frequent. These changes are likely to impact the dynamics between large-scale atmospheric fluctuations and local weather extremes 39 . Deepened AL have been related to changing weather patterns, temperature and wind field fluctuations over North America and Asia 1,2 . In response to an intensified AL and its associated basin-scale cyclonic flows, cold air temperatures, strong northerly winds and stormy conditions dominate East Asia; whereas warmer conditions caused by strong southerly winds are favored on the west coast of North America 1,3,54 . The wintertime North Pacific pressure variability also affects large-scale precipitation changes on the west coast of North America 55 . Here, we present the historical common variability between the intensified AL, SAT and precipitation both spatially and temporally and we further identify the future development of these connections. Air temperature and precipitation conditions in northwest North America are closely related to the AL variability, making them highly susceptible to changes due to an intensified and more frequent extreme AL formation in the future.
As strong and stable atmospheric patterns more commonly emerge in the North Pacific, an intensification of the oceanic response is likely to occur. The AL SLP variability and its extreme deepening have been linked to the Kuroshio Extension net heat flux and SST fluctuations 10,15,19 . The AL controls the variability of the physical parameters in the Kuroshio Extension through basin-scale changes in wind and sea surface height 19,56 . An extreme deepening of the AL increases the westerlies and causes anomalous positive wind stress curl in the central North Pacific 15 . This enhances the southward Ekman drift 11 and produces negative sea surface height anomalies in the central North Pacific. The westward propagation of these anomalies through baroclinic Rossby waves cause lagged responses of the SST of approximately 3-4 years and further destabilize the dynamical state of the Kuroshio Extension system 14,15 . The SST fluctuations in the region generate anomalous heat fluxes 19  www.nature.com/scientificreports/ in an area of maximum ocean-atmosphere heat exchange. Our results suggest that under anthropogenic forcing the common variability of the fields contributing to the above-mentioned mechanism (AL SLP, Kuroshio Extension net heat flux and SST) will substantially increase. Net heat flux and SST in the Kuroshio Extension are also proxies for the strengthening of North Pacific storms 57 . Anomalous heat flux due to fluctuations in the Kuroshio Extension SST front cause changes in the near surface baroclinicity and the lower levels of the troposphere and may result in further genesis of storms in the region 57,58 . Furthermore, changes in the atmospheric pressure conditions and the wind patterns over the North Pacific as well as ENSO events have been correlated with the generation of marine heat waves and extreme sea surface temperature anomalies that governed the area in the last decade 59,60 . The intensification of extreme AL may contribute to explaining the prolonged and more frequent presence of marine heat waves in the region over the past years 61 . It may also assist in further understanding and predicting such events, since changes in the sea level pressure have been directly linked to previous marine heatwave events 62 .
An AL extreme deepening has been previously linked to the major marine regime shift in the North Pacific in the late 1970's 9,10 . Abrupt shifts are predicted to increase in magnitude and consequences under climate change, depending on the severity of the emissions scenario 63,64 . As such, the intensity and frequency of biological shifts in the North Pacific communities could increase in the future, following the late 1970's example 10 . Such shifts have the potential to significantly affect fishing activities 65,66 and have profound socio-economic impacts both on regional as well as global scales [67][68][69] .
Our findings reveal a climate change-induced intensified atmospheric and oceanic variability over the North Pacific, where a strengthening of the AL SLP corresponds to changes in temperature and precipitation patterns and affects the North Pacific oceanic conditions. The potential impacts of such extreme events on the biological and physical conditions of the region stresses the increasing need for continuous monitoring of oceanic conditions, and a rapid advance in our predictive and adaptive capabilities.

CESM1-LENS datasets.
The North Pacific region from 20° N to 60° N and 100° E to 90° W is considered here. The CESM1-LENS is specifically designed to provide information on internal climate variability 36 . All the ensemble members use the same model parameters; however, each member represents a distinctive climate trajectory. This is achieved by initializing the simulations with small round-off differences in the air temperature 50 . Here the daily and monthly SLP, surface air temperature, precipitation, sea surface temperature and net heat flux from 36 members of both the historical and RCP8.5 scenario simulations of the CESM1-LENS from 1920 to 2100 are used. Despite the slightly different initial atmospheric conditions, the ensemble members share the same external historical and future RCP8.5 forcing scenarios and use the same model components. Consequently, the resulting uncertainty in the projections is due to internal climate variability alone, giving the advantage of identifying details of processes, such as the PDO 70 . The effects of AL on the long-term North Pacific climate variability 21,34 highlight the importance of the SLP internal variability, which becomes evident in the multiple simulations of the CESM1-LENS, each of which is forced by an identical scenario of historical and RCP8.5 radiative forcing 42 .
Multiple realizations may also contribute to the understanding of extreme patterns by providing adequate statistical sampling power. The large ensemble size of the CESM1-LENS allows the diagnosis of physical mechanisms for intra-model differences, providing the advantage of accounting for both internal and model variability 36,49 and assessing the statistics of similar events in different realizations that do not reach full agreement with each other. Strong correlations between modeled and observed patterns in NCAR's Climate Analysis Section diagnostics reveal the realistic representation of the system by CESM1-LENS 71 . Specifically for the North Pacific, large-scale patterns such as the Pacific Decadal Oscillation (PDO) are highly related to the PDO index with an average correlation coefficient of 0.86. Furthermore, the SLP is one of best resolved phenomena with an average pattern correlation coefficient of 0.94 between the ensemble members and the observed time-series 71 . Similarly, the total precipitation and SST are two of the best represented parameters with average correlation coefficients of 0.8 and 0.75, respectively. Detailed information about the CESM1-LENS model can be found in Kay et al. 36 .

Dynamical indicators.
To examine the persistence and predictability of extreme events in both past and future simulations of the CESM1-LENS a dynamical approach is applied to all ensemble members. The idea of the approach is that each state of a system x(t) reaches a point ζ on the attractor and its neighbors are all the other states that have a small Euclidean distance with respect to x(t), defined by a threshold q. Dynamical systems exhibiting chaotic dynamics are characterized by strange attractors, i.e. compact geometric objects where the trajectories settle. The existence of attractors ensures the repeatability of a state ζ and the time the dynamics remain in the neighborhood of the state ζ of the system, represented here by the instantaneous dimension d(ζ ) and the inverse persistence θ(ζ) respectively. Specifying these two properties assists in understanding the behavior of the system. These can be estimated by setting a small distance as the threshold, q (2nd and 98th percentile of the time-series) and fitting a Generalized Pareto distribution (GPD) to the tail observations. The approach follows the Peaks Over Threshold method stating that the exceedances above an upper threshold follow a GPD, requiring that the cumulative distribution function of the variable belongs to the max-domain of attraction of the generalized extreme value distribution 72 .
Instantaneous dimension. The extreme values laws are used in this approach in order to characterize the point on the attractor: a fixed point ζ on a chaotic attractor presents a probability P that a trajectory x(t) approaches again the point ζ within a sphere with radius ε centered on ζ. The Euclidean distance between the state ζ and all other observations of the system is: www.nature.com/scientificreports/ where δ x, y is the Euclidean distance between two vectors, which tends to zero when x and y are close. The logarithm calculation increases the discrimination of small values of δ x, y , which correspond to large values of g(x(t)) . The exponential law can describe the probability of logarithmic returns: where location (µ) and scale (σ) parameters depend on the selected point ζ on the attractor. Specifically, σ (ζ ) = 1/d(ζ ) , where d(ζ ) is the instantaneous dimension around the point ζ 73 . Also, q is an upper threshold, and is related to the radius ε of the trajectory of the system via q = g −1 (ε) . Requiring that the series of g(x(t)) is over the threshold q (percentile selection) is similar to the requirement that the trajectory of the system falls within a sphere around the point ζ. Repeating several iterations for different points ζ makes it possible to obtain the dimension of the attractor: where d(ζ ) indicates the instantaneous dimensions averaged over all states ζ.
Inverse persistence. Estimation of inverse persistence in the phase space assists in testing whether the state ζ is in the neighborhood of a fixed point of the attractor or not. If the system were stuck in the same trajectory ( x(t + 1) = x(t) for all t) for an infinite time, then the previous results for the instantaneous dimensions do not hold. Persistence time can be estimated as an additional parameter in the previous law, the extremal index, θ: where θ represents the inverse of the mean residence time within the sphere. Low θ values (close to 0) imply a high persistence of the system, whereas high θ values (close to 1) denote that the trajectory immediately leaves the ζ neighborhood. The value of θ is estimated by using the Süveges maximum likelihood estimator 74 : where N are the observations exceeding a defined threshold, ρ represents the distribution function for the selected threshold, S i is the exceedance distances and N c = N−1 i=1 I(S i � = 0) , where I is the indicator function for the selected S i . For further details on the calculation of the extremal index see ref 74 .
Cross-wavelet coherence. Multi-scale atmospheric and oceanic variability in the North Pacific may present different spatial and temporal ranges, from local spatial events to multi-decadal temporal patterns, nonstationarity and persistence 1,75,76 . As such, quantifying the relationship between the average monthly Kuroshio Extension net heat flux and AL SLP time-series through classic cross-correlation methods that use a defined timelag and which assume independence may give spurious results 77 . Here we use cross-wavelet coherence analysis to detect the time-frequency space in which two time-series present high common power 78 . This approach is based on the time-series decomposition via wavelets and presents the association through phase relationships 79 . The Morlet wavelet is used as the 'mother' wavelet since it balances in an optimum way the localization both in time and frequency 78 . To estimate the significance level at each frequency, Monte Carlo methods were used, in which the wavelet coherence is calculated for pairs of parameters (i.e. SLP and net heat flux) of a large (order of 1000) surrogate dataset with the same AR(1) 78 . The Morlet wavelet used in the wavelet analysis is defined as where ψ 0 (η) is the wavelet function, i is the imaginary unit, ω 0 is dimensionless frequency and η is dimensionless time. The Continuous Wavelet Transform of a time-series x n ( n = 1, . . . , N) with uniform time steps δ t is the convolution of x n with the scaled and normalized wavelet: where W X n (s) 2 is defined as the wavelet power which can be interpreted as the local phase and represents the variance with respect to the frequencies in the signal, ψ 0 is the normalized wavelet, s is the wavelet scale, n is the localized time index, and n ′ the translated time index of the time ordinate x . The global power refers to the time integration of all the local wavelet spectra, if we considered a vertical slice through the wavelet plot as a local measure of the spectrum 80 . The global wavelet power is defined as: (1) g(x(t)) = −log(δ(x(t), ζ )) (2) P g(x(t)) > q, ζ ≈ exp − x − µ(ζ ) σ (ζ ) www.nature.com/scientificreports/ Cross wavelet coherence is analogous to the correlation coefficient in a specified spatial and temporal frequency space where S is a smoothing operator in the scale axis and time domain, and it is defined as where S scale and S time describe smoothing along the wavelet scale and time axes respectively, which should have a similar form to the mother wavelet, the Morlet wavelet here 81 . The statistical significance of the wavelet coherence is tested with Monte Carlo methods 78 .  www.nature.com/scientificreports/