Table 1 Analytical performance characteristics for ELISAs.

From: Serological assessment of SARS-CoV-2 infection during the first wave of the pandemic in Louisville Kentucky

  Spike (S) RBD Nucleocapsid (N)
IgG IgA IgM IgG IgA IgM IgG IgA IgM
Sensitivity 100 94.7 100 94.7 47.4 81.6 84.2 86.8 84.2
Specificity 96.6 100 100 100 100 96.6 76.0 86.2 48.3
Cutoff 0.418 0.297 0.177 0.216 0.125 0.084 0.465 0.101 0.117
  1. ROC analysis to determine ELISA cutoff values (arbitrary units (AU)) with optimal sensitivity and specificity. Data for S IgA, SIgM, RBD IgA, RBD IgM, N IgG, N IgA, N IgM includes a total of 38 RT-PCR confirmed positive patient sera and 29 RT-PCR confirmed negative patient sera for SARS-CoV-2 antibodies. Data for S IgG and RBD IgG includes a total of 38 RT-PCR confirmed positive patient sera and 80 confirmed negative patient sera (RT-PCR or pre COVID).