Obesity and high-fat diet (HFD) consumption result in hypothalamic inflammation and metabolic dysfunction. While the TLR4 activation by dietary fats is a well-characterized pathway involved in the neuronal and glial inflammation, the role of its accessory proteins in diet-induced hypothalamic inflammation remains unknown. Here, we demonstrate that the knockdown of TLR4-interactor with leucine-rich repeats (Tril), a functional component of TLR4, resulted in reduced hypothalamic inflammation, increased whole-body energy expenditure, improved the systemic glucose tolerance and protection from diet-induced obesity. The POMC-specific knockdown of Tril resulted in decreased body fat, decreased white adipose tissue inflammation and a trend toward increased leptin signaling in POMC neurons. Thus, Tril was identified as a new component of the complex mechanisms that promote hypothalamic dysfunction in experimental obesity and its inhibition in the hypothalamus may represent a novel target for obesity treatment.
The hypothalamus is a key brain region involved in the regulation of food intake and systemic homeostasis. In diet-induced obesity (DIO), a chronic and low-grade proinflammatory response in the arcuate nucleus (ARC) of the hypothalamus impairs the neuronal control of energy balance1,2. Thus, to understand how such inflammation develops in the hypothalamus is pivotal in determining obesity treatment.
Saturated fatty acids from hypercaloric diets can serve as ligands for the toll-like receptor-4 (TLR4)3 and genetic mouse models lacking either TLR44 or its downstream accessory protein myeloid differentiation primary response gene 88 (MyD88)5 are protected from DIO and hypothalamic inflammation. Despite progress towards characterization of TLR4 signaling, the role of other critical components of TLR4 signaling in the hypothalamus remain poorly understood.
The TLR4-interactor with leucine-rich repeats (Tril) is a single transmembrane spanning 89 kDa protein that contains 13 leucine-rich repeats and is highly expressed in the brain6. It plays an important role mediating TLR4 signal transduction, and whole-body Tril knockout results in defective inflammatory cytokine production in response to LPS and E. coli, particularly in the central nervous system (CNS)7. A previous study has shown an enrichment of Tril in proopiomelanocortin-expressing (POMC) neurons8, a leptin-responsive and appetite-suppressant group of hypothalamic neurons largely affected by obesity9,10,11. Here, we hypothesized that Tril might be involved in diet-induced hypothalamic inflammation and in the regulation of POMC neurons. We show that knockdown of hypothalamic Tril protects mice from diet-induced body mass gain and systemic glucose intolerance.
Hypothalamic Tril expression is increased in diet-induced obesity
Tril mRNA expression was detected in 44% of POMC neurons and only 25% of AgRP neurons (Fig. 1A,B); the major Tril overlap with POMC neurons was further confirmed in hypothalamic slices from POMC-GFP mice (Suppl. Fig. 1A,D) in comparison with AgRP-tdTomato mice (Suppl. Fig. 1B). Tril was also detected in Iba1-expressing glial cells (Suppl. Fig. 1C). In outbred obesity-prone Swiss mice (Fig. 1C), the hypothalamic (Fig. 1D) but not hippocampal (Fig. 1E) expression of Tril underwent increase one and two weeks after the introduction of HFD. This was accompanied, in the first week, by the increased expression of hypothalamic Tlr4 (Fig. 1F). In isogenic C57BL/6J mice, the hypothalamic expression of Tril was increased after refeeding in mice fed a HFD (Fig. 1G) but not in mice fed a standard chow (Fig. 1H).
Inhibition of hypothalamic Tril protects from diet-induced obesity
Three distinct lentiviral clones containing a shRNA to knockdown Tril were injected in the ARC and a scramble was used as a negative, non-silencing control (Fig. 2A,B); the lentivirus clone #3 (sequence depicted in Fig. 2A and Tril protein inhibition in Fig. 2B) generated a significant Tril knockdown. The protocol (using lentivirus #3) was designed to evaluate whether the knockdown of hypothalamic Tril could prevent the effect of a HFD on metabolic parameters, since the introduction of a HFD occurred after the injection of the lentivirus particles (Fig. 2C). The inhibition of hypothalamic Tril resulted in the reduction of transcript expression of hypothalamic Il1ß, Nlrp3 and Hspa5 (Fig. 2D); no changes were detected in baseline expression of transcripts encoding for Pomc, Agrp and Mch (Fig. 2E). The inhibition of hypothalamic Tril resulted in reduced body mass gain (Fig. 2F) and no change in caloric intake (Fig. 2G and 2H); in addition, inhibition of hypothalamic Tril resulted in reduced absolute (Fig. 2I) and relative (Fig. 2J) epididymal white adipose tissue mass.
Inhibition of hypothalamic Tril improves systemic glucose tolerance
In mice under hypothalamic knockdown of Tril expression and fed a HFD (as depicted in the experimental protocol in Fig. 2C), there was a trend in the increase of energy expenditure (Fig. 3A–C). The absolute (Fig. 3D) and relative (Fig. 3E) mass of the interscapular brown adipose tissue (iBAT) were reduced in comparison to control, but no changes neither in the temperature (Fig. 3F–H) of iBAT nor in the expression of iBAT transcripts encoding proteins involved in thermogenesis (Fig. 3I) were found. Moreover, the inhibition of hypothalamic Tril improved systemic glucose tolerance (Fig. 3J,K), promoted a trend to increase systemic insulin sensitivity (Fig. 3L) and reduced liver steatosis (Fig. 3M), which was accompanied by reduced hepatic expression of transcripts encoding for Scd1 and CD36 (Fig. 3N).
POMC-specific knockdown of Tril reduces body adiposity and increases hypothalamic responsiveness to leptin
A Cre-dependent AAV was designed to selectively knockdown Tril in POMC neurons of the ARC of POMC-Cre mice (Fig. 4A,B). Since the introduction of a HFD occurred after the injection of rAAV, this protocol was designed to evaluate whether the knockdown of Tril in POMC neurons could prevent the detrimental effects of HFD feeding on metabolic parameters (Fig. 4C). This approach resulted in a trend to overcome HFD-induced body mass gain (Fig. 4D,E) and a significant reduction of fat (Fig. 4F) but not lean mass (Fig. 4G). In addition, there were trends to reduce absolute (Fig. 4H) and relative (Fig. 4I) epididymal fat mass and significant reductions of epididymal adipose tissue expression of the inflammatory transcripts Il1β and Tnfα (Fig. 4J). The knockdown of Tril in POMC neurons resulted in a trend to reduce cumulative food intake throughout the experimental period (Fig. 5A) and a significant reduction of food intake acutely after a period of prolonged fasting (Fig. 5B). This was accompanied by increased leptin-induced activation of STAT3 in POMC neurons (Fig. 5C,D) but not in the retrochiasmatic hypothalamus (Fig. 5E). Inhibiting Tril in POMC neurons promoted no modification in the density of αMSH (Fig. 5H, upper panels and Fig. 5I) and AgRP (Fig. 5H, lower panels and Fig. 5I) projections to the paraventricular hypothalamus and it did not alter POMC number (Fig. 5F,G).
POMC-specific knockdown of Tril increases the thermogenic gene expression in brown adipose tissue
No changes in whole-body energy expenditure were observed after POMC-specific knockdown of Tril either in mice fed a chow (Fig. 6A–C) or in mice fed a HFD (Fig. 6D–F). There was a trend to reduce absolute brown adipose tissue (Fig. 6G) but not relative mass (Fig. 6H). Brown adipose tissue temperature was not modified (Fig. 6I–K), but the expression of the thermogenic genes Lhx8 and Zic1 were increased in mice under the inhibition of Tril in POMC neurons (Fig. 6L). In addition, the inhibition of Tril in POMC neurons led to lower fasting blood glucose (Fig. 6M) but no change in whole body glucose tolerance (Fig. 6N). In mice fed a chow, the knockdown of Tril in AgRP-expressing neurons did not change food intake (Suppl. Fig. 4A,B), body weight (Suppl. Fig. 4C), VO2 consumption (Suppl. Fig. 4D), VCO2 production (Suppl. Fig. 4E) and energy expenditure (Suppl. Fig. 4F).
Inhibition of Tril in POMC neurons does not revert the diet-induced obesity phenotype but increases the expression of thermogenic genes in brown adipose tissue
In all preceding experiments, the inhibition of Tril either in the ARC or specifically in POMC neurons occurred two weeks before the introduction of a HFD; thus, the approaches aimed at preventing the harmful effects of the diet. In order to test the hypothesis that the inhibition of Tril in POMC neurons could revert the metabolically adverse effects of long-term consumption of a HFD, mice were fed a HFD for 14 weeks and then injected with the Cre-dependent rAAVmiTril. After 2 weeks of recovery, mice were maintained for another 8 weeks on HFD and metabolic parameters were evaluated (Suppl. Fig. 2A). As depicted in Suppl. Fig. 2B and C, the inhibition of Tril in POMC neurons could neither revert obesity nor change caloric intake in this group of mice. In addition, there were no changes in epididymal fat mass (Suppl. Fig. 2D,E), brown adipose tissue mass (Suppl. Fig. 2F,G) and brown adipose tissue temperature (Suppl. Fig. 2H–J). Nevertheless, in mice submitted to the inhibition of Tril in POMC neurons, there was a trend to increase brown adipose tissue expression of Ucp1 and a significant increase in the expression of Tmem26 (Suppl. Fig. 2K).
Inhibition of Tril in POMC neurons of obese mice did not change the number of POMC neurons in the arcuate nucleus
In experimental obesity, increased apoptosis of neurons in the ARC predominantly affects the number of POMC neurons9,12,13. In obese mice submitted to the protocol of inhibition of Tril in POMC neurons (Suppl. Fig. 2A), there were neither changes in the number of POMC neurons (Fig. 7A,B) nor in the cellular expression of cleaved caspase-3 (Fig. 7C,D).
The stability of body mass depends on the functional and structural fitness of ARC neurons, as demonstrated by a number of different experimental approaches14,15,16. Many advancements have been made towards the molecular programs involved in the obesity-associated hypothalamic inflammation and how the neuronal control of energy balance is affected during the course of HFD feeding3,17,18.
In DIO, POMC neurons are affected at the functional and structural levels by distinct but integrated mechanisms9,19,20,21. Inflammatory signaling pathways are largely associated with DIO-associated POMC abnormalities22,23,24, and its drivers include the activation of RNA stress granules25, endoplasmic reticulum stress3,26, PKCt27 and TLR43,4. The long-term persistence of hypothalamic inflammation, as it occurs when mice are fed a HFD for longer than 8 weeks, leads to a progressive and potentially irreversible deterioration of the melanocortin system28,29. Interestingly, despite the fact that POMC and AgRP neurons share the same anatomical localization, and thus, are potentially exposed to similar harmful factors, POMC undergoes more dramatic changes in response to DIO9,21, raising the possibility that POMC-specific factors could be involved in this increased sensitivity to HFD.
Using a POMC-specific transcriptomics8, we identified Tril, a TLR4-associated membrane protein previously shown to mediate the inflammatory response to LPS in the brain7. Our analysis of in situ mRNA expression revealed that Tril is present in 44% of POMC-expressing neurons and 25% of AgRP-expressing neurons in the ARC. We also employed POMC and AgRP reporter mice to anatomically define the expression of Tril in the hypothalamus and confirmed that Tril is mostly expressed in POMC neurons8. In addition, as it could be expected for a TLR4-associated protein, Tril was detected in microglia30. It has been recently shown that dietary excess facilitates the infiltration of bone-marrow-derived myeloid cells into the ARC and the activation of resident microglia, leading to an inflammatory responsiveness and neuronal and metabolic dysfunction31,32. Using two distinct mouse strains, we showed that hypothalamic Tril mRNA expression is regulated by the consumption of a HFD, as other inflammatory proteins involved in the development of hypothalamic abnormalities in DIO33,34,35,36.
In order to explore the potential involvement of hypothalamic Tril in the development of HFD-associated obese and metabolic phenotypes, we used two distinct approaches. First, we employed a shRNA-based lentiviral clone to selectively knockdown Tril in a non-cell-specific fashion in the arcuate nucleus. Second, we employed Cre-dependent AAV to specifically inhibit Tril in POMC-expressing neurons. When either inhibition was performed in mice fed chow, there were virtually no changes in the phenotype. Conversely, when either inhibition was performed in mice fed a HFD, there were considerable beneficial changes in the obese and metabolic phenotypes. The different outcomes obtained in mice fed the distinct diets support the potential involvement of Tril in the diet-induced inflammatory response.
The action of Tril is similar to CD14 (serving as an accessory molecule that facilitates the binding of LPS to TLR4), mediating at least part of the inflammatory response triggered in this context7. Studies have previously shown that Tril is expressed in glial cells6,7. However, as first shown by Henry and coworkers using RNA-seq of hypothalamic neurons8 and now, confirmed in this study, Tril is also expressed in POMC neurons. One important aspect of TLR4-associated protein expression in the hypothalamus is that, whether CD14 is expressed in glial cells, it is not expressed in POMC neurons37; thus, in this particular cell population, Tril may be essential for the neuronal activation of TLR4.
The non-specific (lentiviral clones) and POMC-specific (Cre-dependent AAV) Tril inhibition in the ARC generated similar but not completely overlapping phenotypes. In both approaches, we found reduction of body adiposity and reduction of BAT mass. In addition, there was a significant reduction of body mass gain after non-specific Tril inhibition and trend for reduction in POMC-specific Tril inhibition. As Tril is also expressed in glial cells7,30 and possibly in other ARC neuronal subtypes, some of the effects obtained in the non-specific Tril inhibition might be related to the diet-induced activation of this protein in metabolism-controlling cells other than POMC neurons. For example, GABAergic/non-AgRP neurons were recently identified as the main leptin-responsive, appetite-suppressant subset of ARC neurons38. A transcriptomic study revealed that short-term HFD activates hunger-promoting prepronociceptin (PNOC) neurons in the ARC and induces transient overfeeding39. Classical neurons, such as those expressing kisspeptin, are involved in the circadian feeding behavior, locomotor activity and thermoregulation40.
Previous reports have shown that the inhibition of distinct components of the HFD-induced inflammatory machinery specifically in POMC neurons, such as Myd88, IKK/NFkB, HIF and TGF-βR2,5,25,41, resulted in beneficial changes in the metabolic phenotype placing POMC as a direct target for the harmful effects of a HFD. Although recent data have shown that leptin signaling in POMC neurons is dispensable for body weight control42,43, the deletion of leptin receptor (LepR) in POMC neurons leads to defective sympathetic innervation of adipose tissue and glucose homeostasis44. Here, we showed that inhibition of Tril resulted in a trend to increase STAT3 phosphorylation, suggesting that preserved leptin signaling in POMC neurons might have a role in the improved fasting glucose and increased expression of thermogenic genes in iBAT of mice fed a HFD.
In the final part of the study, we asked if the POMC-specific inhibition of Tril would revert the detrimental effects of long-term DIO. For that, mice were fed a HFD for 14 weeks before the inhibition of Tril in POMC neurons was undertaken. As previously shown, the metabolic outcomes of feeding mice a HFD for shorter than 8 weeks can be reverted by returning mice to chow; however, longer periods on a HFD lead to irreversible metabolic outcomes28. In concert with human obesity, studies show that the longer patients remain obese, the more severe the clinical outcomes and the more resilient the obese phenotype is, irrespective of the therapeutic approach employed45,46. Here, the inhibition of Tril in POMC neurons in long-term obese mice resulted in no change in body mass, adiposity and food intake. There was only a trend to increase Ucp1 and a significant increase in Tmem26 in BAT, suggesting that, despite the lack of effect on the obese phenotype, the inhibition of Tril in long-term obese mice may retain its thermogenic-inducing effect. In addition, the inhibition of Tril in POMC neurons in long-term obese mice was insufficient neither to change the number of arcuate nucleus POMC neurons nor the expression of cleaved-caspase 3. In conclusion, we found that Tril is a component of the HFD-associated hypothalamic dysfunction and its inhibition may partially restore the central control of peripheral metabolism. Pharmacological approaches aimed at inhibiting Tril in the hypothalamus could provide advance in the treatment of obesity.
Limitations of study
The main limitation of our study is the lack of total and precise deletion of Tril in POMC neurons and some of the marginal effects on metabolic parameters may have a relationship with residual expression of Tril after the knockdown. This gap could be bridged by either crossing a Cre-line with a Tril-floxed mice or employing a selective deletion of Tril in POMC neurons using CRISPR-Cas9.
Six-week-old, male, Swiss and C57BL/6J mice were obtained from the University of Campinas experimental animal breeding facility. AgRP-IRES-Cre mice (#012899, Agrptm1(cre)Lowl/J, Jackson Laboratories) were crossed with the Cre-inducible tdTomato reporter mouse (#007909,B6;129S6-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J, Jackson Laboratories), and POMC-Cre (#005965, Jackson Laboratories) were used in Cre-dependent rAAV experiments or crossed with Cre-inducible GFP-reporter mice (#004178, The Jackson Laboratory) to determine the colocalization of Tril with fluorescently labeled AgRP and POMC neurons, respectively. Mice were housed individually at 22 °C (± 1 °C) using a 12 h light/12 h dark cycle. All mice had ad libitum access to chow (3.7 kcal g−1) or a HFD (60% kcal from fat, 5.1 kcal g−1) and water. For refeeding experiments, mice were deprived of the respective diets for 12 h during the dark cycle and thereafter the hypothalamus was harvested 1, 3, 6, 12 and 24 h after refeeding; these experiments were performed after a five-day washout period to eliminate the novelty and the initial overfeeding associated with HFD introduction. All experiments were performed in compliance with current Brazilian legislation, approved by the Ethics Committee of University of Campinas (#4069-1 and #4985-1) and are consistent with the policies and regulations for animal experimentation described in the ARRIVE guidelines.
To determine O2 consumption, CO2 production and energy expenditure, mice were acclimatized for 48 h in an open circuit calorimeter system, the LE 405 Gas Analyzer (Panlab—Harvard Apparatus, Holliston, MA, USA). Thereafter, data were recorded for 24 h. Results are presented as the average of light and dark cycles. Analysis of covariance (ANCOVA) was used for the comparison of energy expenditure and body mass in mice.
Glucose and insulin tolerance tests
Following 6 h of fasting, mice received intraperitoneal (ip) injections with solutions containing glucose (2.0 g/kg body weight) or insulin (1.5 IU/kg body weight) and then blood samples were collected for ip-glucose tolerance test (ipGTT) or ip-insulin tolerance test (ipITT), respectively. Glucose concentrations were measured in tail blood using a portable glucose meter (Optium Xceed, Abbott) at 0, 15, 30, 60 and 120 min after glucose administration or 0, 5, 10, 15, 20, 25 and 30 min after insulin administration.
Assessment of body composition
To determine total body fat and lean mass, time-domain nuclear magnetic resonance (TD-NMR) was applied using the LF50 body composition mice analyzer (Bruker, Germany). Measurements were performed on the last day of the experiment.
The estimated iBAT temperatures were determined using an infrared (IR) camera (FLIR T450sc, FLIR systems, Inc. Wilsonville, USA) and analyzed with FLIR-Tools software.
Tissue collection and histology
C57BL/6J, AgRP tdTomato and POMC-GFP mice were deeply anesthetized with ketamine (100 mg/kg) and xylazine (10 mg/kg) and submitted to transcardiac perfusion with 0.9% saline followed by 4% paraformaldehyde (PFA). Brains were removed, postfixed 24 h in 4% PFA solution and then transferred to a solution containing 20% sucrose in 0.1 M PBS (pH 7.4) for 12 h. Perfused brains were frozen at − 30 °C and sectioned on a cryostat at a thickness of 30 µm. For immunohistochemistry, free-floating sections were washed three times for 10 min with 0.1 M PBS. Next, sections were blocked in 5% donkey serum (Jackson ImmunoResearch, #017-000-121) and 0.2% Triton X-100 in 0.1 M PBS for 1 h at room temperature, followed by incubation in goat anti-TRIL primary antibody (sc-244489, Santa Cruz Biotechnology, Santa Cruz, CA, USA), rabbit anti-IBA1 (Wako Chemicals, #019-19741), rabbit anti-cleaved caspase-3 (Cell Signaling, #9661S), rabbit anti-POMC (Phoenix Pharmaceuticals, #H-029-30), sheep anti-αMSH (Chemicon, #AB5087) and rabbit anti-AgRP (Phoenix Pharmaceuticals, #H-003-53) for 24 h. The sections were then washed three times in 0.1 M PBS and incubated for 2 h at room temperature in donkey anti-goat AlexaFluor546 (1:500, Invitrogen, #A-11056), donkey anti-goat FITC (1:500, Santa Cruz, sc-2025), donkey anti-rabbit FITC (1:500, Abcam, ab6798), donkey anti-rabbit IgG AlexaFluor594 (1:500, Jackson Immuno Research, #711-585-152) and donkey anti-sheep AlexaFluor488 (1:500, Jackson Immuno Research, #713-545-003) conjugated secondary antibodies. Thereafter, the sections were mounted onto slides, and the nuclei were labeled with TOPRO (Life Technologies, T3605). The sections were analyzed with a LEICA TCS SP5 II confocal laser-scanning microscope (Leica Microsystems, Wetzlar, Germany).
Multiplex fluorescent in situ hybridization (FISH)
Brains from C57BL/6J mice were quickly harvested and frozen in OCT at − 20 °C and stored at − 80 °C. The slides containing a 15-µm-thick brain sections were processed for multiplex FISH using the RNAscope system (ACDBio) and probes for the following mRNA: Tril (C-1), Pomc (C-2) and AgRP (C-3). The pretreatment was performed by sequential incubations of slides in 4% PFA, 50% EtOH, 70% EtOH and 100% EtOH at room temperature. Probe hybridization was achieved by incubation of 30 µl mRNA target probes for 2 h at 40 °C using a HyBez oven. The signal was then amplified by subsequent incubation of Amp-1, Amp-2 and Amp-3 for 30, 30 and 15 min, respectively, at 40 °C using a HyBez oven. Each incubation step was followed by RNAscope washing buffer washes. The slides were coverslipped and mounted using DAPI Fluoromount-G (SouthernBiotech). Slides were visualized with an inverted Zeiss LSM 780 laser scanning confocal microscope using a × 20 lens.
Mice were submitted to 12 h of fasting and then received ip injections with mouse leptin (5 mg/kg body weight, Calbiochem, Billerica, MA, USA). After 1 h, mice were submitted to transcardiac perfusion with 0.9% saline followed by 4% PFA. After sectioning on a microtome, the brain sections were rinsed in 0.02 M KPBS (pH 7.4), followed by pretreatment in a water solution containing 1% hydrogen peroxide and 1% sodium hydroxide for 20 min. After extensive washings in 0.02 M KPBS, the sections were incubated in 0.3% glycine for 10 min and then 0.03% lauryl sulfate for 10 min. Thereafter, the sections were blocked in 3% normal donkey serum for 1 h, followed by incubation in rabbit anti-pSTAT3Tyr705 (1:1000, Cell Signaling, #91315) for 48 h. For the immunofluorescence reactions, sections were rinsed in KPBS and incubated for 120 min in Fab fragment donkey anti-rabbit AlexaFluor488 (1:500, Jackson Immuno Research, #711-547-003). Thereafter, sections were washed three times in 5% formalin for 10 min and then washed three times in 0.02 M KPBS for 5 min. Sections were then incubated with POMC antibody (1:1000. Phoenix Pharmaceuticals, #H-029-30) overnight at room temperature. After washing in 0.1 M PBS, the sections were incubated for 30 min with the secondary antibody AlexaFluor594 (1:500, Jackson Immuno Research, #711-585-152) diluted in 0.02 M KPBS. After three washes in 0.02 M KPBS for 10 min, the sections were mounted onto gelatin-coated slides and coverslipped with Fluoromount G (Electron Microscopic Sciences, Hatfield, PA, USA). The percentage of pSTAT3-positive POMC neurons was determined in blind counting by three distinct researchers using hemisections of the middle ARC from four animals for statistical comparison. The sections were processed simultaneously under identical conditions and analyzed with the same microscope set-up.
Liver histological analysis
Fragments of left lobe were collected and fixed in 10% buffered formalin, embedded in paraffin and stained with hematoxylin and eosin (H&E, Merck, USA), and analyzed and photographed using a light microscope (Axio Observer D1, Zeiss, USA).
Three different shRNA-based U6-driven lentiviral clones targeting Tril—TRCN0000191275 target sequence: CCAGTATCTTACTGTGTTATT; TRCN0000201889 target sequence: CTGGATTACCTGGATGACCAA; TRCN0000202144 target sequence: CCAACATCTCCTATGCACCAA (Sigma-Aldrich, St Louis, MO, USA) and one scramble (non-targeting) lentiviral particles were used for the overall knockdown experiments. For arcuate nucleus (Arc) bilateral lentiviral delivery, 8–12 week old male C57BL/6J mice were anesthetized with ketamine (100 mg/kg body weight) and xylazine (10 mg/kg body weight) intraperitoneally, and the stereotaxic surgery was carried out using a stereotaxic frame (Stoelting Apparatus, Wood Dale, IL, USA) set at AP − 1.7 mm, ML ± 0.3 mm and DV − 5.6 mm coordinates from Bregma.
Cre-dependent recombinant adeno-associated viral (rAAV) vectors
With regard to the POMC-specific knockdown of Tril, referred to here as POMC rAAVmiTRIL, a TRIL-based miRNA construct was constructed by modifying the Cre-dependent AAV-FLEX-EGFP-mir30 (Scn9a) driven by CAG promoter (Addgene plasmid # 79672). Briefly, the plasmid was modified by replacing the self-complementary sequences of Tril shRNA, using EcoRI and Xhol. All miR sequences were preserved. The sequence used was the following, where complementary sequences are underlined: 5′CGAGGCAGTAGGCACCAGTATCTTACTGTGTTATTTACA TCTGTGGCTTCACTAAATAACACAGTAAGATACTGGCGCTCACTGTCAACAGCAATATACCTT-3′. The rAAV particles were produced in the LNBio (Brazilian Biosciences National Laboratory) facility and titrated using an Addgene protocol by qPCR. Briefly, purified AAV particles were treated with DNAse by incubation for 30 min at 37 °C. A standard curve was generated using the AAV-FLEX-EGFP-mir30 (TRIL) plasmid serial diluted. SYBR Green quantitative PCR (qPCR) was performed using the primers: fwd ITR primer 5′-GGAACCCCTAGTGATGGAGTT-3′ and rev ITR primer 5′-CGGCCTCAGTGAGCGA-3′. The Cre-dependent bilateral injections of the rAAV vectors in the Arc were performed in POMC-Cre mice at the following stereotaxic coordinates: AP − 1.7 mm, ML ± 0.3 mm and DV − 5.6 mm from Bregma. All AAVs were allowed 2 weeks for expression before experiments were initiated.
Gene expression analysis
Total RNA was extracted from the hypothalamus, inguinal and epididymal white adipose tissue, brown adipose tissue and liver using TRIzol reagent (Invitrogen). cDNA synthesis was performed using 2 µg of total RNA. The PCR containing 25 ng of reverse-transcribed RNA was performed using the ABI Prism 7500 sequence detection system (Applied Biosystems). For RT-PCR calculation, the delta CT was used, and the relative gene expression was normalized to that of GAPDH in all samples. In Fig. 1E,F and G the mRNA expression of Tril in mice fed on HFD was also normalized for the respective control group of mice fed on chow. The primers used were Tril (Mm01330899_s1), Ucp1 (Mm01244861_m1), PGC1α (Mm01188700_m1), Zic1 (Mm00656094_m1), LHX8 (Mm00802919_m1), Tfam (Mm00447485_m1), PPARγ (Mm01184322_m1), Scd1 (Mm00772290_m1), Srebf1 (Mm01138344_m1), Cd36 (Mm01135198_m1, Tnfα (Mm00443258_m1), F4/80 (Mm00802529_m1), Il1-β (Mm00434228_m1), Nlrp3 (Mm00840904_m1), Hsp90 (Mm00441926_m1), Hspa5 (Mm00517691_m1), Caspase-3 (Mm01195085_m1), Pomc (Mm00435874_m1), AgRP (Mm00475829_g1), Mch (Mm01242886_g1), Tmem26 (Mm01173641_m1), Th (Mm00447557_m1), Ccl2 (Mm99999056_m1) and Tbx1 (Mm00448949_m1).
Hypothalamic specimens were homogenized in solubilization buffer (1% Triton X-100, 100 mM Tris (pH 7.4), 100 mM sodium 22 pyrophosphate, 100 mM 4 sodium fluoride, 10 mM EDTA, 10 mM sodium vanadate, 2 mM 23 PMSF and 0.1 mg/mL 5 aprotinin). A total of 100 µg of protein per sample were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE), transferred to nitrocellulose membranes and blocked in 3% BSA solution in TBST for 2 h. After a washing step, the membranes were blotted with goat-anti TRIL antibody (sc-24489, Santa Cruz Biotechnology, Santa Cruz, CA, USA), and α-tubulin (Sigma-Aldrich, T5168) was used as loading control. Specific bands were labeled by chemiluminescence and were quantified by optical densitometry after exposure to Image Quant LAS4000 (GE Healthcare, Life Sciences).
Results are presented as mean ± standard error of the mean (SEM). Statistical comparisons between different times of refeeding and controls were performed using analysis of variance (ANOVA), followed by Tukey’s post hoc test. Student’s t tests were applied for comparisons between scramble and KDTRIL in experiments with lentiviral clones or POMC CremiNonTarget and POMC CremiTRIL in experiments with Cre-dependent rAAV.
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Alexandre Moura-Assis received financial support from the São Paulo Research Foundation (FAPESP #2016/01245-5 and #2019/12969-2). Pedro A. Nogueira received financial support from Coordenadoria de Aperfeicoamento de Pessoal de Nivel Superior through the graduate Course in Cellular and Structural Biology, Insititute of Biology, University of Campinas. The authors thank Erika Roman, Joseane Morari, Marcio Cruz and Gerson Ferraz for laboratory management.
LAV, São Paulo Research Foundation (FAPESP—2013/07607-8). FMS, São Paulo Research Foundation (FAPESP—2018/14818-9). JDJr, São Paulo Research Foundation (FAPESP—2017/02983-2). Conselho Nacional de Desenvolvimento Cientifico e Tecnológico, INCT-Neuroimmunomodulation. Coordenadoria de Aperfeiçoamento de Pessoal de Nível Superior.
The authors declare no competing interests.
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Moura-Assis, A., Nogueira, P.A.S., de-Lima-Junior, J.C. et al. TLR4-interactor with leucine-rich repeats (TRIL) is involved in diet-induced hypothalamic inflammation. Sci Rep 11, 18015 (2021). https://doi.org/10.1038/s41598-021-97291-7