Figure 3 | Scientific Reports

Figure 3

From: PacBio sequencing output increased through uniform and directional fivefold concatenation

Figure 3

Overview of the post-sequencing data processing. The CCS function in the SMRT Link software v8.0 assimilated the consensus sequences from each well and removed the SMRTbell hairpin adapters. We developed a custom script to sort (demultiplex) and deconcatenate the sequences. Firstly, the sample-specific barcodes were used to separate each selection output from the mixed pool of sequences. Next, we extracted the five original library variants from the concatenated amplicon using the forward and reverse constant region at the 5’ and 3’ termini of each variant (not shown in the figure). A length filter was then applied to ensure the recovery of full-length library variants. See Supplementary Fig. S4 for further detail.

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