Dietary carbohydrates interact with AMY1 polymorphisms to influence the incidence of type 2 diabetes in Korean adults

The relationship between AMY1 single nucleotide polymorphisms (SNPs), dietary carbohydrates, and the risk of type 2 diabetes is unclear. We aimed to evaluate this association using an ongoing large-scale prospective study, namely the Korean Genome and Epidemiology Study. We selected six genetic variants of the AMY1 gene: rs10881197, rs4244372, rs6696797, rs1566154, rs1930212, and rs1999478. Baseline dietary data were obtained using a semi-quantitative food frequency questionnaire. Type 2 diabetes was defined according to the criteria of the World Health Organization and American Diabetes Association. During an average follow-up period of 12 years (651,780 person-years), 1082 out of 4552 (23.8%) patients had type 2 diabetes. Three AMY1 SNPs were significantly associated with diabetes incidence among patients with carbohydrate intake > 65% of total energy: rs6696797, rs4244372, and rs10881197. In multivariable Cox models, Korean women with the rs6696797 AG or AA genotype had 28% higher incidence of type 2 diabetes (hazard ratio 1.28, 95% confidence interval 1.06–1.55) than Korean women with the rs6696797 GG genotype. We did not observe significant associations between AMY1 SNPs, dietary carbohydrates, and diabetes incidence in Korean men. We conclude that AMY1 genetic variants and dietary carbohydrate intake influence the incidence of type 2 diabetes in Korean women only. Korean women who are minor carriers of the AMY1 rs6696797, rs4244372, and rs10881197 genotypes may benefit from a low-carbohydrate diet to prevent the future risk of type 2 diabetes.


Scientific Reports
| (2021) 11:16788 | https://doi.org/10.1038/s41598-021-96257-z www.nature.com/scientificreports/ increased 12 . Low variation in AMY1 copy number is correlated with high insulin resistance in healthy Korean men 13 . AMY1 copy number and carbohydrate intake differentially influence the development of type 2 diabetes. Single nucleotide polymorphisms (SNPs) near amylase genes are highly correlated with AMY1 copy number 14 . Thus, nonsynonymous SNPs in the AMY1 gene may be an additional genetic influence on amylase expression 5 . It is important to explore both SNPs and copy number variations when analyzing the genetic basis of disease risk 15 . Although the link between AMY1 copy number variations and the risk of type 2 diabetes has been widely explored, few studies have examined the associations between AMY1 genetic variants, dietary carbohydrate intake, and the incidence of type 2 diabetes. Understanding how these factors interact will provide insight regarding the role of genetic polymorphisms in type 2 diabetes etiology, with implications for further research on the physiological effect of AMY1 SNPs. In particular, examining the role of AMY1 genetic polymorphisms in the digestion of dietary carbohydrates is of great importance for improving our understanding of the type 2 diabetes pathophysiology. Using a prospective cohort study, we tested the hypothesis that AMY1 genetic polymorphisms and carbohydrate intake interact to alter the incidence of type 2 diabetes.

Participant baseline characteristics.
During a follow-up period of 12 years on average, 1082 out of 4552 (23.8%) patients developed type 2 diabetes. General characteristics of the subjects grouped by carbohydrate intake are shown in Table 2. Participants with carbohydrate intake > 65% of total energy intake were more likely to live in Ansan, were non-smokers, older, more physically engaged, and less likely to drink than participants with carbohydrate intake ≤ 65%. Those with > 65% carbohydrate of total energy also had lower intake of total energy, total protein, animal protein, fat, sugar, and dietary fiber, and expectedly, had a higher intake of carbohydrates. We also found lower fasting glucose levels in participants with carbohydrate intake > 65% of total energy intake.
Relationship between AMY1 SNPs, dietary carbohydrates, and incidence of type 2 diabetes in men. Table 3 shows the adjusted HRs and corresponding 95% CIs for the prospective association between six AMY1 SNPs and diabetes incidence by carbohydrate intake in Korean men. After adjusting for age, residence area, education level, smoking status, alcohol consumption, physical activity, BMI, and family history of diabetes, we did not observe significant associations between individual AMY1 SNPs and the incidence of type 2 diabetes in either low-or high-carbohydrate groups in men.
Relationship between AMY1 SNPs, dietary carbohydrates, and incidence of type 2 diabetes in women. Table 4 presents the adjusted HRs and corresponding 95% CIs for associations between AMY1 SNPs and incidence of type 2 diabetes by carbohydrate intake among Korean women. Three AMY1 SNPs were significantly associated with the incidence of type 2 diabetes in women with > 65% carbohydrate intake from energy: rs6696797, rs4244372, and rs10881197. In the > 65% group, women with the rs6696797 AG or AA genotype had a 28% higher incidence of type 2 diabetes (HR 1.28, 95% CI 1.06-1.55) than women with the rs6696797 GG genotype. Women with the rs4244372 AT or AA genotype had a 29% higher incidence of type 2 diabetes than those with the TT genotype (HR 1.29, 95% CI 1.07-1.56). Women with the rs10881197 GC or GG genotype had a 30% greater incidence of type 2 diabetes than those with the CC genotype (HR 1.30, 95% CI 1.08-1.57).
Cumulative incidence of type 2 diabetes by AMY1 rs10881197 genotypes and dietary carbohydrates. Figure 1 shows the Kaplan-Meier plot of the cumulative type 2 diabetes incidence, grouped by AMY1 rs10881197 genotypes (CC vs. GC or GG) and carbohydrate intake groups (≤ 65% or > 65% energy). Among the carbohydrate intake ≤ 65% group, cumulative incidence of type 2 diabetes was 10%, 17.9%, and 23.9% in 5, 10, and 15 years, respectively, for individuals with the rs10881197 CC genotype. For those with the rs10881197 www.nature.com/scientificreports/ GC or GG genotype, cumulative incidence of type 2 diabetes was 12%, 19.2%, and 24.1% in 5, 10, and 15 years, respectively. Among the carbohydrate intake > 65% group, the cumulative incidence of type 2 diabetes was 8.1%, 16.3%, and 21.9% in 5, 10, and 15 years for those with the rs10881197 CC genotype, respectively. For the rs10881197 GC or GG genotype, the cumulative incidence of type 2 diabetes was 9.7%, 18.7%, and 24.9% in 5, 10, and 15 years, respectively. Overall, the cumulative incidence of type 2 diabetes was the highest in participants who had a carbohydrate intake > 65% and carried the rs10881197 GC or GG genotype.

Discussion
In this average of 12-year, prospective population-based cohort study of middle-aged Korean adults, we demonstrated that carriers of minor alleles in AMY1 (rs6696797, rs4244372, and rs10881197) had significantly elevated incidence of type 2 diabetes if they were women who obtained > 65% of their total energy intake from carbohydrates. To the best of our knowledge, this is the first study to test the hypothesis that AMY1 genetic variants, coupled with higher carbohydrate intake, are associated with a higher incidence of type 2 diabetes. Notably, we only observed these significant associations in women and not in men, suggesting a potential sex-specific mechanism related to sex hormones or sex chromosome complement 16 . The Toronto Nutrigenomics and Health Study reported that AMY1 rs10881197 and AMY1 copy numbers were both associated with lower energy intake among 20-29-year-old Caucasian adults 17 . Thus, AMY1 SNPs may contribute to starch and energy intake. Perry et al. 5 reported that although AMY1 protein expression is correlated with AMY1 copy number, the latter does not fully explain a considerable degree of variation in the expression of the AMY1 protein (R 2 = 0.351). Therefore, other genetic factors, such as regulatory-region SNPs, could influence AMY1 expression. Both AMY1 copy number and AMY1 SNPs may explain between-individual differences in AMY1 protein expression, and accordingly, the reason for some individuals having a higher risk of developing type 2 diabetes than others. Here, we observed the greatest disease incidence in women who consumed high-carbohydrate diets and possessed the A allele of rs6696797, A allele of rs4244372, and G allele of rs10881197. In healthy, non-obese individuals, those with low amylase activity did not have increased insulin levels before glucose absorption began, whereas Table 2. General subject characteristics, stratified by carbohydrate intake. Data are presented as mean ± standard deviation or n (%). P values are from chi-square tests for categorical variables and t-tests for continuous variables. SD standard deviation, MET metabolic equivalent task, HOMA-IR homeostatic model assessment of insulin resistance.   www.nature.com/scientificreports/ individuals with high amylase activity had lower postprandial blood glucose concentrations 9 . Evolutionarily, individuals with high AMY1 copy numbers and salivary amylase activity have more rapid and efficient starch digestion mechanisms, which ensure the quick availability of blood glucose for extreme energy expenditure activities like farming or hunting 9 .
A previous study reported that high AMY1 copy number is associated with enhanced glucose absorption following an oral glucose load 19 . The authors suggested that the co-evolution of high AMY1 copy number with increased carbohydrate resulted in an enhanced salivary digestion of starch to maltose, followed by conversion to glucose. This enhancement optimized the efficiency of glucose absorption across the upper gastrointestinal tract. Thus, both genetic and dietary factors are implicated in the development of type 2 diabetes. Here, we found that the cumulative incidence of type 2 diabetes was highest at year 15 for participants regardless of the rs10881197 genotypes, but significant difference in cumulative incidence by rs10881197 genotypes (CC vs. GC or GG) was found only in the high carbohydrate-intake group. Cumulative incidence at year 15 was lowest among the participants with the rs10881197 CC genotype and their counterparts in the low-carbohydrate-intake group. This result emphasizes the combined effects of AMY1 genotypes and dietary carbohydrates on the incidence of type 2 diabetes. Type 2 diabetes is strongly linked with obesity 9 , a major cause of insulin resistance 20,21 . Hence, a growing body of literature has investigated the relationship between AMY1 copy number [22][23][24][25][26][27][28] and obesity, but the outcome has been inconsistent, either failing to find any associations [22][23][24] or identifying an inverse relationship [25][26][27][28] . These inconsistencies may be partially due to between-study differences in techniques for evaluating the AMY1 copy numbers and in the racial/ethnic compositions of the subject populations. Although the link between AMY1 copy numbers and obesity remains uncertain, AMY1 copy number is associated with salivary amylase concentrations 6 , and this relationship may contribute to individual differences in dietary starch intake. Chronically elevated blood glucose, induced by high starch intake, may cause hormonal, receptor, and physiological changes that would eventually result in type 2 diabetes 9 . Furthermore, a diet rich in sugars impaired cardiac systolic and diastolic function in the mouse 29 . Besides SNPs in AMY1 gene, several SNPs in susceptibility genes have been associated to insulin sensitivity or insulin resistance. As genes involved in adipose tissue metabolism may influence insulin sensitivity, the polymorphisms of adiponectin gene (−11,391G>A, − 11,377C>G, and +45T>G) were associated with insulin resistance state in overweight/obese children 18 . It is significant to explore a wide variety of genetic factors for insulin resistance for future studies.
Our study has several limitations. First, we lacked the data to analyze salivary amylase concentration; thus, we could not further explore the linkage between AMY1 SNPs and salivary amylase concentrations. Additionally, the study was limited to participants who were over 40 years old and living in Ansan or Ansung. Therefore, our findings may not be applicable to the general Korean population.
Nevertheless, this study has several strengths. To our knowledge, it is the first to examine the association between AMY1 genetic variants and the incidence of type 2 diabetes after the stratification of the study population based on carbohydrate intake, using a 16-year prospective follow-up design. We were also able to control for multiple confounding variables because KoGES provided relevant health-and disease-related phenotypic data 30 . Finally, exploring the genetic basis of type 2 diabetes risk requires measuring all forms of genetic variation, including SNPs. Accordingly, this work contributes to expanding the existing knowledge on the combined role of AMY1 and dietary carbohydrates on the development of type 2 diabetes.
In conclusion, we demonstrated that the AMY1 genetic variants and dietary carbohydrate intake influenced the incidence of type 2 diabetes among Korean women. The underlying mechanisms linking the genes, diet, and disease remain unclear, but our findings suggest that AMY1 SNPs and dietary factors are both important for type 2 diabetes etiology. This study provides a sound empirical basis for screening individuals who are minor carriers of AMY1 (rs6696797, rs4244372, and rs10881197) and have a high-carbohydrate diet, to detect future risk of type 2 diabetes. Figure 1. Cumulative incidence of type 2 diabetes by carbohydrate intake. (A) Comparison of cumulative incidence between rs10881197 CC and rs10881197 GC or GG genotypes for carbohydrate intake ≤ 65% of total energy intake. (B) Comparison of cumulative incidence between rs10881197 CC and rs10881197 GC or GG genotypes for those with carbohydrate intake > 65% of total energy intake. , we excluded those who did not participate during the follow-up at least once (n = 912), had a diagnosis of type 2 diabetes at baseline (n = 1098), had a diagnosis of cancer (n = 192), lacked dietary information (n = 253), had energy intake < 500 kcal/day or > 5000 kcal/day (n = 66), had missing data for confounding variables (n = 104), or had no SNP data (n = 2853). The final analytical sample comprised 4552 individuals (Fig. 2). The KoGES study was reviewed and approved by the Institutional Review Board of the Korea Centers for Disease Control and Prevention. All participants enrolled in the study voluntarily and all gave written-informed consent. All study methods and protocols were conducted in accordance with the relevant institutional guidelines and regulations. The protocol was reviewed and approved by the Institutional Review Board of Inha University on January 31, 2020 (IRB No. 2001291A).
Dietary assessment. Dietary data were collected by well-trained interviewers at baseline, using a 103 item semi-quantitative food frequency questionnaire (FFQ). This validated FFQ was developed to assess the usual dietary intake of Korean adults who participated in the KoGES 31 . All participants were asked how often they consumed each food item during the previous year. They could give nine possible responses ranging from never or seldom to ≥ 3 times per day 31 . To calculate the typical intake of foods and nutrients, including carbohydrates, consumption frequency for each unit of food was multiplied by the nutrient content of each food item, by referring to a nutrient database (CAN-Pro 2.0) developed by the Korean Nutrition Society 32 . For this study, carbohydrate intake (g/day) and percentage contribution in terms of energy (% energy) were assessed using the FFQ. Assessment of type 2 diabetes. During the biennial follow-up examination, type 2 diabetes was categorized as fasting blood glucose ≥ 126 mg/dL after at least 8 h of fasting and 2 h plasma glucose level of 75 g oral glucose tolerance test ≥ 200 mg/dL, in accordance with the criteria of the World Health Organization 33 and American Diabetes Association 34 . Other acceptable criteria were diagnosis of diabetes by a physician, use of insulin treatment, or use of diabetic medication.
Men and women were analyzed separately. Subjects were categorized into two groups: ≤ 65% carbohydratederived energy (maximum AMDR for carbohydrates) and > 65%, according to the Dietary Reference Intakes for Koreans 2020 36 . Baseline sociodemographic and lifestyle characteristics of participants were compared using chisquare tests for categorical variables and ANOVA for continuous variables. Hazard ratios (HRs) and 95% confidence intervals (CIs) for the incidence of type 2 diabetes were estimated with Cox proportional hazard models, using individual AMY1 SNPs under a dominant genetic model stratified by carbohydrate intake (≤ 65% or > 65%). Covariates were age (years), area of residence (Ansan or Ansung), education level [elementary school or lower (< 7 years of www.nature.com/scientificreports/ school completed), middle/high school (7-12 years), college or higher (> 12 years)], smoking status (never, former smoker, current smoker), alcohol consumption (g/day), physical activity [metabolic equivalent task (MET)-h/week], body mass index (BMI; kg/m 2 ), and family history of diabetes (determined from self-reports: yes, no). For each AMY1 SNP, we further employed Kaplan-Meier plots to examine the cumulative incidence of type 2 diabetes during follow-up periods, stratified by carbohydrate intake (≤ 65% and > 65%). All statistical analyses were performed in SAS software (version 9.4; SAS Institute, Cary, NC, USA). Significance was set at P value < 0.05 (two-sided).