Genomic epidemiology of SARS-CoV-2 in the UAE reveals novel virus mutation, patterns of co-infection and tissue specific host immune response

To unravel the source of SARS-CoV-2 introduction and the pattern of its spreading and evolution in the United Arab Emirates, we conducted meta-transcriptome sequencing of 1067 nasopharyngeal swab samples collected between May 9th and Jun 29th, 2020 during the first peak of the local COVID-19 epidemic. We identified global clade distribution and eleven novel genetic variants that were almost absent in the rest of the world and that defined five subclades specific to the UAE viral population. Cross-settlement human-to-human transmission was related to the local business activity. Perhaps surprisingly, at least 5% of the population were co-infected by SARS-CoV-2 of multiple clades within the same host. We also discovered an enrichment of cytosine-to-uracil mutation among the viral population collected from the nasopharynx, that is different from the adenosine-to-inosine change previously reported in the bronchoalveolar lavage fluid samples and a previously unidentified upregulation of APOBEC4 expression in nasopharynx among infected patients, indicating the innate immune host response mediated by ADAR and APOBEC gene families could be tissue-specific. The genomic epidemiological and molecular biological knowledge reported here provides new insights for the SARS-CoV-2 evolution and transmission and points out future direction on host–pathogen interaction investigation.

www.nature.com/scientificreports/ Genomic epidemiology using massively parallel high-throughput sequencing technologies (MPS) and associated analyses and bioinformatics tools have been used to understand the rapid spread and evolution of the virus at a larger scale than ever before 8,9 . Public repositories including GISAID have enabled fast release and sharing of SARS-CoV-2 genome sequences 10 . Those efforts provide valuable information to researchers and public health officials for global outbreak responses. Nevertheless, there are new questions arising regarding the virus' ongoing breadth of transmission, its evolution inter-and intra-host, as well as host-pathogen interactions. The genetic diversity of global viral strains is largely underestimated given the lack of real-time sequencing capability in most of the world, resulting in a disproportional under-study of viral populations in under-and recently-developed countries. As a consequence, there is limited information on novel and common genetic variation in those areas where virus rapidly evolves and is subjected to natural selection, as it encounters human hosts with diverse genetic background and an environment with varying temperature and humidity levels 11,12 . Most published research since the start of the pandemic has focused on inter-host phylogenetics based on the assumption that only one strain of the virus is present in the sample. Intra-host viral genetic diversity and the prevalence of coinfection has not been established via sufficiently large cohort despite the possibility that it might impact clinical outcomes and potentially enable higher resolution analysis in the who-infects-whom transmission chain 13 . Finally, while understanding how the host response to the virus will help to combat the disease, innate immune response process such as the host-dependent RNA-editing mechanism has only been investigated among limited sample cases 14 .
The United Arab Emirates (UAE) is one of the world's most famous international hubs for business and travel and is the first country to approve a Chinese COVID-19 vaccine. Despite a long-lasting period of epidemic, only a few of the SARS-CoV-2 samples were sequenced and the transmission and evolution patterns of the virus in this area is unknown. The first case of SARS-CoV-2 was detected in the country on January 29th, 2020 (Fig. 1). The subsequent outbreaks infected over sixty thousand individuals by the end of June 2020 and three hundred thousand individuals by the end of December 2020 3 . Since March 2020, the UAE public health authorities have adopted a series of strict regulations to reduce human-to-human transmission, including airport lockdown and national curfew. On the other hand, due to economic pressures, a few international flights reopened gradually in June 2020, which may be one of the reasons for the subsequent small second peak during June and August. The most outstanding third epidemic peak were observed during the December Christmas time in 2020. There have been 2-4 thousand newly confirmed cases in the country since Christmas. Since the very beginning, as a response to the pandemic, several high-throughput molecular technologies have been adopted in the UAE to extensively monitor the viral spread and for rapid screening of infected patients. A nationwide RT-qPCR screening program conducting ten thousand tests daily was launched on March 31st, 2020. Almost simultaneously, a high-throughput sequencing laboratory with 12-18 Tbases/day capacity was established in early April 2020, enabling meta-transcriptome sequencing of up to 192 samples in 24 h.
To understand the transmission and infection dynamics of SARS-CoV-2 within the UAE and in relation to other countries, during April and July, 2020, we randomly collected 1067 nasopharyngeal specimens from SARS-CoV-2 positive patients from the RT-qPCR screening program and conducted meta-transcriptomic sequencing. Our main scientific questions include (1) What is the virus genetic diversity and transmission pattern in the UAE during the first peak of the epidemic (2) What is the extent of co-infection of multiple SARS-CoV-2 variants in this international travel hub (3) Is there any innate immune host response to the SARS-CoV-2 infection that can be detected using the meta-transcriptomic sequencing, which contains both the host and the viral gene expression information.

Results
Assembly and variant detection of SARS-CoV-2 genome from deep meta-transcriptome sequencing of 1067 nasopharyngeal swab samples. A total of 1067 nasopharyngeal swab samples collected from SARS-CoV-2 positive patients between May 7th and June 29th 2020 in Abu Dhabi were sequenced (Fig. 1A). Their sequencing quality metrics were summarized in Fig. S1 and Table S1. We obtained high quality assemblies (gap proportion < 2%) for the majority of the samples (n = 896, 84.0%). In brief, using the 29891 nt SARS-CoV-2 reference genome (IVDC-HB-01), we have successfully assembled all 1067 SARS-CoV-2 consensus genomes as follows-896 assemblies with gaps less than 500 nt (gap proportion < 2%), 27 assemblies with gap less than 1000 nt (gap proportion < 4%), 14 assemblies with gaps less than 1500 nt (gap proportion ~ 5%) and 130 assemblies with gaps greater than 1500 nt (Fig. 1B). As expected, quality of the genome assemblies was closely related to the sample viral load as measured by reads per million (RPM) and qRT-PCR Ct values (Fig. 1B,  Fig. S2). A set of three samples (id:0555, 0919 and 0945) showed low viral loads (Ct < 19) with unexpectedly poor assemblies (gaps > 1500 nt), likely due to RNA degradation as many of the sequenced reads were filtered out due to low complexity, i.e. high polyA proportion (Table S1).
The distribution of gaps identified in the sequences indicates low sequencing coverage over the 5′ and the 3′ ends of the genomes, which was found to be a common occurrence in all world-wide assemblies reported in GISAID. We also notice a significantly higher number of gaps around the 20,000 nt position for 27.1% of the assemblies submitted to GISAID, which were not observed in our assemblies (Fig. S3). Among the selected 896 assemblies with the highest quality (gap proportion < 2%), we identified a total of 1245 genetic variants consisting of 698 non-synonymous and 547 synonymous variants when compared to the SARS-CoV-2 reference genome (IVDC-HB-01) (Fig. 1C, Table S2). The number of variants per sample ranged from 1 to 24 with a median number of 11 (Fig. S4). Very few genomes carried non-single nucleotide variants. There was one 2 nt insertion in one sample 1069 and six deletions identified in fourteen samples 0188,0236,0252, 0290, 0305, 0339, 0512, 0536, 0757, 0758, 0761, 0763, 0785 and 1092, the largest being a 4 nt deletion present in 7 of the 14 samples (Fig. S5). The consensus variants identified from the technical replicates were exactly the same (Table S3)   www.nature.com/scientificreports/ 4% alternative allele frequency threshold, the concordance rate of intra-host genetic variant detection reaches 100% (Fig. S6). The number of variants that we identified per sample did not correlate with the sequencing depth (squared pearson correlation coefficient R 2 ~ 0.02) (Fig. S7).
Global clade composition and five novel subclades associated with eleven novel common genetic variants in the UAE SARS-CoV-2 population. Likely due to fast population expansion with a short period, we discovered that 395 out of the 896 genomes (44.1%) assembled in our study shared an identical genome sequence with at least one other assembled genome (Table S4). For the purpose of downstream phylogenetic analysis, we filtered the 896 genome sequences as to keep only unique sequences, resulting in 637 unique genome sequences. We constructed a maximum likelihood phylogenetic tree including, (1) the 637 SARS-CoV-2 unique genomes and collected assembled in our study between May 7th and June 29th, 2020 in Abu Dhabi, (2) the 52 nearest relative world-wide genomes identified from GISAID between February 2nd and April 24th, 2020 (Table S6, Fig. S8), and (3) 25 genomes collected from the nearby Dubai Emirate between January 29th and March 18th, 2020 15 . We identified the five dominant clades worldwide 16,17 in the UAE viral population sequenced in this study ( Fig. 2A) (Table S6). The high level of mutations occurring in these samples compared to the rest of the UAE genomes, indicates a different introduction of strains within the same clade. There were five large sub-clades involving more than half of the collected samples (381 out of the 637 unique viral genomes, 59.81%) ( Fig. 2A), differentiated by eleven mutations that were common in the UAE viral population (allele frequency > 5%) and that were significantly less common among the worldwide viral population (P < 3.94e − 82, Fisher exact test) (Fig. 2B, Table 1). The five sub-clades were (1)  Fortunately, individuals classified as carrying certain subclades of the virus did not display significantly different viral loads in their samples as reflected by the RT-qPCR Ct values (Fig. 3). These 11 variants that defined the subclades tend to occur in highly conserved regions within the SARS-CoV-2 genome (Fig. S9). Molecular dynamic analysis of two of the missense variants in the spike protein did not suggest substantially different change of the protein structure between the mutant and the wildtype (Fig. S10, Table S7). Likely due to a recent occurrence, the temporal change of the mutation allele frequency for the subclade-definitive variants is smaller compared to the clade-definitive variants (Figs. S11 and S12).

Cross-settlement human-to-human transmission contributes to the UAE epidemic.
We further investigated human-to-human transmission across 14 settlements from three regions in the Abu Dhabi Emirate and 1 settlement in the Dubai Emirate by constructing the transmission network for 120 samples with geographical and sampling date information (Fig. 4A). The constructed transmission network indicates prevalent cross-settlement human-to-human transmissions contributing to the epidemic, as within each clade or sub-clade, samples from multiple geographical areas were observed (Fig. 4B). We also determined the genetic distance using the L1-norm metric that utilized intra-host genetic variation rather than merely the consensus genetic variation, among longitudinal samples (n = 24) defined as, same individuals (n = 7) sampled multiple times (avg = 5.2) over a determined period of time (avg = 4.06 days), and among samples from the same and varying settlements (Fig. 4C). The median L1-norm genetic distance was smallest among the 24 samples within the longitudinal sampling period, suggesting high levels of stability in viral composition within the same host. As expected, most samples within the same settlement had a genetic distance smaller than the cross-area distance with only two exceptions-samples from the Ghayathi settlement in the AI-Dhafra region and samples from Khabisi in the Dubai emirate, that displayed the largest genetic distance. This is consistent with the fact that those two settlements were relatively less populous compared to the settlements in the Abu Dhabi and AI-Ain regions. The spectrum and the scale of the L1-norm genetic distance is much larger than the genetic computed from the consensus genetic variants although the haplotype information is missing. Due to the small scale of sampling, we did not further resolve the transmission network to a finer scale.

Prevalent co-infection by multiple SARS-CoV-2 variants in the same host. The international
hub status of the UAE provides a good opportunity to study the prevalence of multiple SARS-CoV-2 variant coinfection within the same host. We have identified a total of 1268 intra-host single nucleotide variation (iSNV, with minor allele count of 4 and minor allele frequency greater than 5%) present in 625 out of the 896 samples, ranging from 1 to 26 iSNV per individual with an average of one per individual (Fig. S13). Although the technical replicates indicate 100% concordance of the iSNV detection at the above threshold, we chose a conservative way of evaluating the prevalence of multiple infection present in the sampled viral population by restricting the definition of co-infection by the co-occurrence of two clades including 19A, 19B, 20A, 20B and 20C (classified using the eleven clade-definitive variants in Fig. 2) or subclades (classified using the other eleven sub-clade www.nature.com/scientificreports/ 19B (C8782T and T28144C) www.nature.com/scientificreports/ definitive variants) in the same sample. We found that a total of 48 samples out of the 896 (5%) carried viral variants from more than two distinct clades or subclades (Fig. 5) (6) Al Shahama(4) Al Shamkha (5) Baniyas (9) Ghayathi (3) Hayer (2) Khabisi (2) Markhaniya (4) Mezyad (2) Mutaredh (3) Salamat (2) Sila (2) Wagan (2) Yahar ( Table S5, ten samples were not displayed because only one sample were collected from that settlement. The UAE map was obtained from the world-geo.js file (https:// gist. githu buser conte nt. com/ munaf-zz/ 46302 18/ raw/ 32a38 9a88f 990e0 1c2c7 661c5 51c84 af9ed a1a26/ world-geo. js) and plotted using jQuery JavaScript Library v1. 11 www.nature.com/scientificreports/ Fig. 5 largely maintain when using a 0.5% minor allele frequency threshold and the same 4 minor allele support (Figs. S14 and S15), showing a tremendous amount of intra-host genetic diversity underlying the consensus genomes of the host. For seven out of the eight samples, they identified a bias of the mutation towards transition, mainly A>G/T>C changes followed by C>T/G>A changes, indicating a deamination effect introduced by ADARs and APOBECs, respectively (WH BALF in Fig. 6A). In the nasopharyngeal swab sampling of 896 patients in our study, on the contrary, we identified the C>T/G>A as the predominant SNV type that were more likely to be mediated by APOBEC gene family rather than the A>G/T>C effects mediated by the ADARs (UAE in Fig. 6A). This held true when only mutations that occurred in more than two patients were considered. As expected, the C-to-U changes are biased toward the positive strand, i.e. more C-to-U was observed compared to G-to-A, as APOBECs are supposed to target single stranded RNA 20 . The observation of a dominant C-to-U changes were replicated in the nasopharyngeal swab samples collected in Spain, Virginia and Ruijin hospitals in Shanghai city, China and the 23164 high quality sequences collected in GISAID ("Supplementary Notes"), which consistently displayed an enrichment in the C>T/G>A mutations, same as the pattern in the UAE nasal swab samples but different from the Chinese BALF results reported by Giorgio et al. (Fig. 6A). Additional evidence can be obtained with the observation of cytosine depletion in viral sequences during the past ten months, reflected by an increasing of T and A bases and a decreasing of G and C bases (Fig. S16). We further investigated if the different patterns observed could be due to the differential gene expression of the APOBEC gene families and ADAR in the nasopharyngeal swab vs. BALF using public multi-tissue gene

The innate immune host response to SARS-CoV-2 infection may be tissue-specific and associ-
C1059T G25563T  www.nature.com/scientificreports/ expression information from GTEx repository 21 and by analyzing the gene expression of APOBEC and ADAR genes in our sequencing data. According to the GTEx gene expression data among 49 tissues and cells, ADAR demonstrated the highest gene expression compared to APOBEC gene family in the lung and in the minor salivary gland, the two most relevant tissue compared to the nasopharynx used in our study (Fig. S17). The GTEx information cannot directly explain the different mutation pattern between the BALF and the nasal swab samples. Distinct from the GTEx profile obtained from the uninfected individuals (Fig. S17), APOBEC4(A4) displayed the highest average gene expression in the nasal swab samples collected in our study, followed by ADAR and APOBEC3A, while there were very few samples expressed APOBEC1, APOBEC2 and APOBEC3H (Fig. 6B). The difference of gene expression is significant between A4 and the ADAR (Wilcoxon test P = 7.7e − 05) and the largest difference was observed among the individuals carrying clade 20A variants followed by the clade 19B variants (Fig. 6B, Table S8). In GTEx, A4 is expressed most prominently in testis, lowly expressed in lung and infrequently expressed in other tissues (Fig. S17).
The significantly up-regulated A4 gene expression in the nasopharynx could have been triggered by the SARS-CoV-2 infection. A4 was an under-studied putative cytidine-to-uridine editing enzyme, which cytidine deaminase activity was not as well-known as the APOBEC3A 22 . The sequencing data not aligned to the SARS-CoV-2 were filtered out from the BALF samples and therefore, we were not able to investigate the gene expression of those host genes in this tissue. That the A4 was previously reported to enhance the replication of HIV-1 indicates its involvement against the RNA virus infection. The high expression of A4 in nasopharynx may provide the first evidence that the enzyme may be involved as part of the host responses upon the SARS-CoV-2 infection and further experimental analysis is worthwhile to understand its exact functions.

Discussion
Our analysis of the 1067 viral genomes collected in the UAE suggest that, during the first quarter of 2020, there were multiple and likely independent introductions of SARS-COV-2. The five dominant global clades of SARS-CoV-2 were all commonly present in the sampled individuals (Fig. 2). The highest prevalence of the European dominant clade 20B, followed by the East Asian dominant clade 19B, indicates effects of either a larger founder population size or positive selection. There was substantial local transmission within and between areas in the Abu Dhabi emirate (Fig. 4). We have identified five new sub-clades, namely; 19B.1, 20B.1, 20B.2, 20B.3 and 20B.4, defined by 11 variants uniquely found within the UAE. Those variants are potentially neutral given that no significantly different viral loads (reflected by the RT-qPCR test) were detected between patients carrying the subclades and those did not (Fig. 3).
While consensus sequences tend to be highly similar, intra-host variation adds information which is a promising novel direction for resolving finer-scale transmission networks and studying co-infection of the patients. This study offers the first insight into the prevalence of co-infections of multiple SARS-CoV-2 strains in a large cohort. We observed that at least 5% of the patients were infected by more than one SARS-CoV-2 strain. Within-host co-infection of SARS-CoV-2 variants has been reported in very few studies and with limited sample size. The environment created by the UAE's "international hub" status also enables a reliable approach to study co-infection within an individual by different strains of SARS-CoV-2 using clade and sub-clade definitive genetic variants. This raises the importance of carefully collecting valuable epidemiological data worldwide, on the origin and clinical relevance of the multiple infections, and the possibility of further granularity when studying transmission dynamics by utilizing information from multiple strains.
While this study showed that SARS-CoV-2 successfully mutated in the two-month period collection in the United Arab Emirates, it is clear that a large number of mutational changes have taken place in the past 10 months of this pandemic. This would likely result in an immunologic battle between host response and changes in the viral genome potentially leading to important structural changes. We observed a significant accumulation of C-to-U mutations in the nasopharyngeal swab samples collected in this study compared to the early stages of sampling around the globe. This pattern is different to what has been reported in a recent study where an enrichment of A-to-G was followed by T-to-C mutations in seven out of eight BALF samples from Wuhan 19 . We suspect that tissue-specific gene expression of ADAR and member of the APOBEC protein family may contribute to this observation and discovered that APOBEC4 was highly expressed in the nasopharynx. Given that APOBEC4 was previously reported to enhance RNA virus replication and was mainly expressed in Testis in an ordinary status, it will be interesting and worthwhile to understand more about its exact function towards the SARS-CoV-2 infection using experimental analysis.
The genomic epidemiological insights from our study will provide a strong basis for the surveillance of emerging mutations within the local viral population. Following the gradual reopening of borders and worldwide travels, the continuous sequencing and identification of allele frequency changes of those variants and additional experimental validation are necessary to verify their biological impacts. Future efforts will be aimed at speeding up the process in providing near real-time molecular surveillance and in the coordination of epidemiological and genomic data to rapidly adapt to SARS-CoV-2 evolution to ensure public safety, adequate diagnosis and accurate pharmaceutical development.

Methods
Study design and population. Patients with positive RT-qPCR SARS-COV-2 diagnosis are referred to local designated hospitals administered by the Abu Dhabi Health Services Co (SEHA) and the Department of Health in Abu Dhabi (DOH) for quarantine and treatment. Through a routine surveillance system, all cases of SARS-CoV-2 are reported to the DOH.
In this population-based retrospective study, we have randomly selected 1067 patients testing positive for SARS-CoV-2 during the months of May and June 2020, regardless of their clinical symptoms. We collected the Genome assembly. The BetaCoV/Wuhan/IVDC-HB-01/2019|EPI_ISL_402119 sequence was used as the virus reference genome. The IVDC-HB-01 reference lacks 12 A nucleotides at the end compared to Wuhan/ Hu-1/2019 and consists of 24 more sequences at the 5′ beginning compared to Wuhan/WH01/2019. SARS-CoV-2 consensus sequences were generated using Pilon v1.23 (parameters: -changes -vcf -changes -vcf -mindepth 10 -fix all, amb). Nucleotide positions with sequencing depth < 10× were masked as ambiguous base N. We have also applied de novo assembly of the Coronaviridae-like reads from samples with < 100× average sequencing depth using SPAdes (v3.14.0) with the default settings. The Coronaviridae-like reads of samples with > 100× average sequencing depth across SARS-CoV-2 genome were subsampled to achieve 100× sequencing depth before being assembled. However, the assembled genomes are enriched of errors and therefore we did not use those assembled sequences in the downstream analysis.

Consensus variation detection and annotation.
Pilon generates a variant calling formatted file for recording the consensus variation. To verify the correctness of those consensus variation calls, we also applied freebayes (v1.3.1) (parameters: -p 1 -q 20 -m 60 -min-coverage 10 -V) to detect genetic variation from the bam file. The low-confidence variants were removed with snippy-vcf_filter (v3.2) (parameters: -minqual 100 -mincov 10 -minfrac 0.8). The correctness of those results was evaluated using the two technical replicates (Table S3). The remaining variants in VCF files generated by freebayes were annotated in SARS-CoV-2 genome assemblies and consensus sequences with SNVeff (v4.3) using default parameters. Jalview (v1.8.3) was used to perform multiple sequence alignment and estimate the conservativeness score of the mutations.

Intra-host variation detection.
We applied reditools to compute the sequencing depth of the four A, C, G, T bases (parameters: python2.7 reditools.py -f sample.bam -o sample.count.txt -S -s 0 -os 4 -r ref.fa -q 25 -bq 35 -mbp 15 -Mbp 15). The intra-host genetic variation was detected using reditools 24 with a minimum frequency of 5% and 4 copies of minor alleles. We have applied three technical replicates for two samples to evaluate the accuracy of the assembled sequence, the consensus and intra-host genetic variants. This conservative cutoff was decided based on the two sets of technical replicates with examination of concordance (SNV found in both samples) and discordance (SNV found in only one of the two samples) for different frequency thresholds.

Analysis of host ADAR and APOBEC gene expression.
Reads were aligned to the human genome reference (GRCh38) using hisat2 (parameters: -phred64 -no-discordant -no-mixed -I 1 -X 1000 -p 4). Reads aligned to the exons defined by UCSC (gencode.v29.annotation.gtf) were counted (parameters: -s no -f bam -t exon -m union -r name -i gene_id). TPM was defined by the following formula where where x refers to a gene or a transcript. R refers to the read length, C x indicates the number of read pairs aligned to the exons of the gene x. T indicates the length of the gene (kb) divided by the total length of all the genes (kb). L x indicates the length of gene x. www.nature.com/scientificreports/ Phylogenetic analysis and cross-area transmission inference. From the total 896 assembled highquality genomes (< 2% gap proportion), 637 were unique, therefore considered as different strains, and were used for further phylogenetic analysis. These were aligned to 46917 genome sequences collected outside of the UAE between January 10th and June 16th, 2020 and deposited to the GISAID EpiCoV database (https:// www. epicov. org/). As subset of genome sequences were selected for phylogenetic tree building, including the 637 strains sequenced in this study, the 52 most closely related genome sequences from the alignment analysis against the global 46,917 sequences, and 25 genome sequences also obtained from GISAID that were collected and sequenced in Dubai, UAE, from January 29th to March 15th, 2020. We built a maximum likelihood phylogenetic tree using the Nextstrain pipeline; Augur v6.4.3 and MAFFT v7.455 for multiple sequence alignment and IQtree v1.6.12 for phylogenetic tree construction 17 . FigTree v1.4.4 was used to visualize and annotate the phylogenetic tree. Clades were defined following the Nextstrain nomenclature 16 . Subclades were further defined in this study based on common variants (> 5%) in the UAE but is significantly rarely present in the rest of the world (fisher exact p-value < 4e − 82).
Samples with corresponding epidemiological data including patients' addresses and date of first sample collection were also used to generate median-joining networks for each clades and subclades using PopART (Population Analysis with Reticulate Trees) v1.7. L1-norm genetic distance was computed using the formula previously defined in the influenza study by Poon et al. 13 , reflecting the sum of the degree of difference for each variant nucleotide position of any two samples (see below). L1-norm genetic distance. We calculated the L1 genetic distance between sample pairs using the following formula.
For position k, d k was the distance measured for comparing base frequency vectors p and q from two samples. n is the total number of nucleotide configurations (A, C, G, T). For each pair of samples, D represented the sum of the difference in all positions, and N is the sum of the number of variants.
We calculated D for all pair-wise samples and compare the median between groups defined by areas.
Statistical analysis. Fisher exact tests were applied to the 637 unique genomes identified in this study and to 23,164 SARS-CoV-2 genomes collected worldwide from GISAID and curated in the China National Center for Bioinformation (CNCB) 25 . The tests were used to identify variants that display substantial allele frequency differences between the two sets of genomes sequences; UAE vs. rest of the world. Kruskai-Wallis test was used to compare the RT-qPCR Ct values between clades and subclades.
The distribution of the ten types of genetic mutations (e.g. A>C, C>G mutations) as well as the base contents for all 4 nucleotides (A, C, G and U) as a function of time was used to infer the RNA-editing functions of ADAR and APOBEC proteins within the host. The enrichment of a specific type of mutations were tested using chisq tests.
Mutation analysis related to the host response. The URL for data resources in investigating the nucleotide changes from Ruijin, Virginia, Spain, Wuhan and GISAID were detailed in "Supplementary Notes". Molecular dynamics simulation. The original structures (PDB format) of SARS-CoV-2 proteins were downloaded from Protein Data Bank (PDB, https:// www. rcsb. org/) with accession numbers, ORF3a: 6xdc, Spike: 6vyb and NSP12:7bv2. Point mutations were introduced into each protein sequence and generated the mutated sequence. The mutated sequence and the corresponding original template protein structure were then taken as inputs for SWISS-MODEL for Homology modeling. After the modeling was completed, the PDB files of the target mutated proteins were obtained for further analysis. Subsequently, Ions and waters are deleted from PDB files. The PDB files were then subjected to GROMACS 26 (Version: V5.1) and utilized for molecular dynamics simulation at the temperature 300 K. Gromacs output the free energy (KJ/mol) to measure the stability of candidate protein. A smaller value of free energy indicates a higher stability of protein.

Data availability
A total of 896 high quality consensus assemblies (with less than 2% gaps) were submitted to GISAID (EPI_ ISL_698105-698169, EPI_ISL_698172-699161, EPI_ISL_708827-708838) and raw sequencing data aligned to the SARS-CoV-2 reference genome were uploaded to NCBI (PRJNA687136). We combined our genomes with other publicly available sequences for a final dataset of 973 SARS-CoV-2 genomes (ncov_global.json, Supplementary file). The dataset can be visualized on the ''community'' Nextstrain page.