Pelvic floor dysfunction one year after first childbirth in relation to perineal tear severity

The aims of this study were to evaluate pelvic floor dysfunction symptoms one year after delivery and investigate whether adverse functional outcomes after childbirth were related to the degree of perineal injury. A prospective cohort of 776 primiparas were included. Self-reported pelvic floor function data were obtained using a web-based questionnaire. Women with no/first-degree injuries, second-degree injuries, third-/fourth-degree injuries (obstetric anal sphincter injury, OASI) and cesarean section were compared. A total of 511 women (66%) responded. Second-degree tears were a risk factor for stress incontinence (aOR 2.6 (95% CI 1.3–5.1)). Cesarean section was protective against stress incontinence (aOR 0.2 (95% CI 0.1–0.9)). OASI was a risk factor for urge incontinence (aOR 4.8 (95% CI 1.6–15)), prolapse (aOR 7.7 (95% CI 2.1–29)) and pelvic pain (OR 3.3 (95% CI 1.1–10)). Dyspareunia was reported by 38% of women, 63% of women in the OASI group (aOR 3.1 (95% CI 1.1–9.0)). Women with OASI reported that the injury affected daily life (OR 18 (95% CI 5.1–59)). Pelvic floor dysfunction is common after childbirth, even in women with moderate injury. Women with OASI had significantly higher risks of symptoms of prolapse, urge urinary incontinence, pain, dyspareunia and impacts on daily life.

www.nature.com/scientificreports/ The demographic and obstetric characteristics of the responders in the three different perineal trauma groups and the cesarean section group are presented and compared in Table 2. There were no differences among the three injury groups regarding maternal age, body mass index, infant head circumference, gestational age at delivery, induction rate, labor augmentation or epidural use. The duration of the active secondary stage of labor was significantly longer in patients with second-degree injuries than in those with an intact perineum or first-degree injuries. There was a significant difference in infant birthweight between patients with no or minor perineal trauma and OASI, with the highest infant birthweight among women affected by OASI. Occiput posterior presentation was more common in the OASI group than in the other groups, but the difference was not significant after post hoc testing.
Obstetrical vacuum extraction was most common in those with second-degree injuries. Mediolateral episiotomy was performed in 20.0% (85/424) of all women, resulting in iatrogenic injury in 45.5% (80/175) of the women with second-degree tears. Among women with second-degree lacerations, episiotomy was more common in deliveries by vacuum extraction (73.5% 25/34) than in spontaneous deliveries (38.7%, 55/142, p < 0.001). In the OASI group, the episiotomy rate was 26.3%, and none of the deliveries were vacuum assisted. There were no forceps deliveries.
Symptoms of prolapse were reported in 8.3% of the primiparas one year after delivery. OASI was a risk factor for developing symptoms of prolapse (aOR 7.7, Table 3). In total, 6.2% of the patients had to insert a finger in the vagina to assist in emptying their bowels.
Urinary stress incontinence was present in 31.0% of women, and 18.0% suffered from urge incontinence. Second-degree trauma was a risk factor for stress incontinence (aOR 2.6, Table 3), and giving birth by cesarean section was protective against stress incontinence (aOR 0.2, Table 3). The risk for urge (aOR 4.7, Table 3) incontinence was elevated in the group with the largest injuries. An impact on lifestyle was reported in 12.1% of the women with urinary incontinence, and in women with OASI, the risk of urinary incontinence that affected their lifestyle was significantly elevated (Table 3).
Anal incontinence was experienced by 13.9% of women. Severe incontinence with leakage of solid stool was reported after only vaginal delivery. The severity of the symptoms was more prominent among women in the OASI group, who also had an increased risk of anal incontinence affecting their lifestyle, than among women in the other groups (OR 23, Table 3).
Most of the women were sexually active, although 9.7% of the women had not resumed sexual relations. Four women chose not to answer the question. Dyspareunia was experienced by a large proportion, accounting for 38.3% of the women who were sexually active. The rate of dyspareunia ranged from 31.3-41.4% in the groups with first/second-degree injuries and cesarean section and reached 62.5% in the group with OASI. The risk of Table 2. Demographic and obstetric characteristics of analyzed responders in the three different groups of perineal trauma and cesarean section. Data are the mean ± SD or n (%). *na = not analyzed. www.nature.com/scientificreports/ experiencing dyspareunia was elevated in women with OASI (OR 3.1, Table 3). The feeling of being too tight (14.6%) was more common than the feeling of being too loose (8.6%). Perineal pain was experienced by 11.6% of women, and 21 women reported that pain that was severe enough to prevent most activities in the last 3 months. This type of severe pain was present in all groups, with an elevated risk in the OASI group (OR 3.3, Table 3). OASI was a risk factor for symptoms originating from the injury that had an impact on daily activities (OR 18, Table 3). Patients with OASI had the highest rate of complications.

Discussion
In this study, we evaluated pelvic floor function one year after birth in a nonselected cohort of primiparas and found that adverse functional outcomes were experienced in women with perineal lacerations of all grades as well as those with an intact perineum. Dyspareunia and urinary incontinence were the most common symptoms of pelvic floor dysfunction one year after childbirth. Those who suffered from OASI had a significantly higher risk of experiencing symptoms of prolapse, urinary urge incontinence, anal incontinence, dyspareunia and pain. Patients with OASI also had a significant risk of their injury impacting daily life one year after giving birth. Notably, women with moderate perineal lacerations (second-degree and episiotomy injuries) reported various symptoms of perineal dysfunction one year after delivery, and second-degree trauma was a risk factor for urinary stress incontinence.
Women who underwent cesarean section generally had a low incidence of dysfunction apart from dyspareunia (31%), for which the prevalence was in line with that in women with an intact perineum. Cesarean section was also protective against stress incontinence. The results may have been affected by the fact that this was an allencompassing cesarean section group including women who underwent elective (35%) and emergency cesarean sections; nevertheless, the prevalence of perineal dysfunction seemed to be lower in this group than in the other groups. Similar findings have been reported in evaluations of pelvic floor function 5 and 10 years after birth, in which cesarean section deliveries were associated with significantly lower hazards for stress urinary incontinence, overactive bladder, and pelvic organ prolapse 8 . However, birth by cesarean section is associated with a risk of pelvic floor morbidity coupled to the surgery itself 9 .
Pain during intercourse was reported in 38% of the women, which is in line with data from previous studies (22.4-37.3%) 6,10 . The etiology of dyspareunia is multifactorial; scar formation after perineal laceration is one possible cause; other causes include inflammatory states, infections, and lack of sexual arousal 11 . The incidence of dyspareunia has been reported to increase after childbirth, from 12% before pregnancy to 31% six months after birth 12 . Breastfeeding has traditionally been used by midwives and gynecologists as an explanation for postpartum dyspareunia and was found to be a risk factor for dyspareunia at 6 months after birth in a recent study 13 . In Sweden, more than 70% of mothers have ceased breastfeeding at 12 months after birth 2 . To obtain in-depth knowledge of sexual function after childbirth, one needs to assess prepregnancy function and perform repeated follow-ups with a validated assessment tool, such as the pelvic organ prolapse/incontinence sexual questionnaire-IUGA revised (PISQ-IR) 14 . www.nature.com/scientificreports/ In our study, the frequency of urinary incontinence was lowest in the subgroup who underwent cesarean section and was highest among patients with major injuries. Symptoms of prolapse were most prominent in patients with OASI. A previous study showed that vaginal delivery was associated with higher risks of prolapse and urinary incontinence than cesarean delivery 15 .
A similar trend was observed for anal incontinence. Excluding patients with OASI, severe anal incontinence with leakage of stool was found in only women with vaginal deliveries, which might indicate that they had undiagnosed sphincter injuries 16,17 . Various studies have postulated that the frequency of undiagnosed OASI is high, at 11-29% [16][17][18] . Women with OASI reported a significant impact of symptoms on daily life. Other studies have also shown that anal incontinence results in a decrease in overall quality of life 19 .
We recognize that there are limitations to our study due to the inclusion of measurements of rather subtle pelvic floor dysfunction characterized by symptoms occurring as little as once a month. There were no objective measures, such as in-person examinations, during follow-up. On the other hand, functional impairment is an easy way to identify patients who need further evaluation and treatment. In the aftermath of childbirth-related trauma, early identification of pelvic floor dysfunction enables us to intervene with effective strategies (such as pelvic floor rehabilitation) to prevent subsequent aggravation of pelvic floor dysfunction requiring surgery. Predicting and preventing long-term morbidity due to injury to the pelvic floor will also decrease healthcare costs 20 .
There are a number of biases inherent to investigations utilizing questionnaires that we tried to minimize by using a shortened version of an already existing and validated national questionnaire 21 . One possible bias is that respondents experiencing symptoms were more likely to respond, but those experiencing symptoms were comparable to the nonrespondents in the majority of baseline characteristics. The responders were older than the nonresponders, representing a potential source of responder bias given that age is a risk factor for pelvic floor dysfunction, but responder bias is unlikely due to the minor differences. Confidence intervals were wide in the OASI group due to an OASI prevalence of 4.5%, leading to a small sample size (n = 19).
The strengths of this study include its prospective cohort design and the nonselected study population since the hospital is the only one in the area. Higher fetal weight, a longer secondary active state and occiput posterior position are well-known risk factors for both OASI 22 and levator trauma 23 . Patients who stated that they were pregnant at the time of the questionnaire were excluded from the analysis of symptoms to ensure that the results were not affected by the impact of pregnancy on function; consequently, the population eligible for analysis was substantially reduced.
The average rate of vacuum extraction in the country is 9.5%; in this study, the prevalence was 11.1%. Responders had a higher rate of vacuum delivery than nonresponders, creating possible bias due to more traumatic birth experiences. The episiotomy rate was markedly higher than the national average, at 20% in this study compared to 10% nationally 2 . On the other hand, vacuum delivery and episiotomy were not risk factors for pelvic floor dysfunction in this study. Vacuum extraction is associated with an increased prevalence of OASI and second-degree tears 24 . Episiotomy was more common in vacuum deliveries than in other types of deliveries, probably because clinical practice has been influenced by studies suggesting the reduced risk of OASI when performing episiotomy in vacuum deliveries in nulliparas 25 . The benefits and risks of routine episiotomy have been widely debated 26 . Recent studies have concluded that selective episiotomy is recommended when normal delivery is anticipated 27,28 . A reduction in the episiotomy rate in unassisted vaginal deliveries led to an increase in second-degree injuries of anatomic damage less than or equal to that caused by episiotomy without and increased rate of severe second-degree injuries 28 . There is continuing support for the use of episiotomy in vacuum delivery in nulliparas 29 although the number needed to treat was 27, and the results from randomized controlled trials to validate these recommendations are awaited 30 . Episiotomy is an obstetric technique associated with levator injury 23 . Trauma to the levator ani during vaginal childbirth is associated with female pelvic organ prolapse 31 and has been suggested but not confirmed to have an association with symptoms of urinary and anal incontinence 32,33 . Regarding sexual function, a recent study found no association between postpartum levator hiatus and sexual dysfunction 34 .
In conclusion, OASI is an evident risk factor for pelvic floor dysfunction after childbirth, but symptoms of pelvic floor disorder were found to be common, even in women with mild to moderate perineal laceration. Second-degree lacerations have a wide range of perineal tear extension and a subclassification may be helpful further to stratify the risk of subsequent pelvic floor dysfunction 28 . An elevated risk associated with a larger injury was observed in our study, and women with OASI reported a significant impact of their symptoms on daily life. This indicates that strategies should remain focused on preventative measures and improved diagnostics for large perineal lacerations.
Since pelvic floor disorders are stigmatizing conditions causing great physical and emotional suffering, measures aiming to reduce postpartum morbidity and to improve women's sexual health are of great importance. The association between perineal trauma and levator trauma 35 and the increased risk of long-term pelvic dysfunction with composite trauma constitutes an important area for further research to increase confidence in vaginal births and mitigate the increased demand for cesarean sections.

Methods
This study was part of a large interdisciplinary project involving birth-related perineal injuries, endoanal ultrasonography and postpartum pelvic floor disorders. The present study is a follow-up study in the cohort of the aforementioned project. The project aimed to prospectively include all nulliparous women who gave birth from January 2016 to January 2018 at Östersund Hospital. This is the only hospital in the county of Jämtland-Härjedalen, Sweden, and approximately 98% of pregnant women residing in the area give birth at this hospital. Written consent to participate in the study was obtained at the routine fetal anomaly scan at 18-20 weeks of pregnancy. www.nature.com/scientificreports/ Women who agreed to participate in the study were clinically examined immediately after birth to diagnose perineal injury. The clinical examination was performed by a midwife or, in eligible cases, by a doctor, and consisted of inspection and bidigital palpation in the lithotomy position. The laceration grade was documented by the midwife or doctor according to the International Classification of Diseases Tenth Revision (ICD-10) World Health Organization (WHO) guidelines (Table 4). Only mediolateral episiotomy was performed. The findings were documented in a study protocol. Data on maternal, obstetric and neonatal characteristics were obtained from medical records.
Participants who were one year postpartum received an invitation to complete a follow-up questionnaire by mail. An information sheet that explained the purpose of the study, security standards, data storage, and the consent process and provided the contact information of the responsible investigators was included with the letter. Participants were asked to complete a web-based questionnaire regarding symptoms of prolapse, urinary incontinence, anal incontinence, and perineal pain as well as sexual function. The questionnaire consisted of 15 questions and contained the most important items of the questionnaire for the postoperative evaluation of perineal injury provided by the National Swedish Society of Obstetrics and Gynecology 21 . The scored items of the questionnaire were validated. The questionnaire was available in only Swedish (native language). All questions had "no" or "never" as response options, and for questions about sexual function, patients had the option of not answering without being excluded from the analysis. Adaptive questioning processes were used in cases where supplementary questions were required to obtain further details on occurring symptoms, such as anal incontinence. Completion of the survey was voluntary. The patients provided informed consent by logging in with their individual codes and submitting the results. Participants received two reminders by text message and/ or telephone call. Answers were automatically recorded in a database to which only the responsible investigators had access.
The participants were divided into three groups depending on the extent of perineal injury (no/first-degree injury, second-degree injury, and OASI). Women who underwent cesarean section comprised the fourth group. The period prevalence of self-reported symptoms of prolapse, urinary and fecal incontinence, dyspareunia and perineal pain were calculated based on the proportion of women who reported symptoms that matched the study definitions divided by the total number of women with available data for follow-up. In cases of multiple birth, the study considered the infant with the highest birthweight. Prolapse was defined as the feeling of something bulging out of the vagina or the need to press against the posterior vaginal wall to empty the bowel once a month or more. Stress urinary incontinence was defined as incontinence once a week to daily, urge incontinence as once a month to daily. Impact on daily activity was defined as noticeable symptoms once a month or more often. Involuntary passing of flatus was defined as the involuntary passing flatus once a month or more. Leakage of stool was defined as stool leakage once a month to daily. Sexually active was defined as intercourse within the last three months. Perineal pain was defined as pain that is impossible to ignore and that affects concentration on daily tasks and activities. The statement "very satisfied" or "satisfied" was used to determine satisfaction with suturing.
Statistical analyses were performed by analysis of variance for continuous data (ANOVA). The chi-square test or Fisher's exact test was used as needed for categorical data, and the independent-sample t test was used for normally distributed continuous data. When analysis with the chi-square test was performed with more than two groups and statistical significance was found, pairwise testing and post hoc testing was performed to verify the finding. Odds ratios were calculated with binary logistic regression analysis, with 95% confidence intervals (CIs). Odds ratios were adjusted for age, body mass index (BMI) and fetal weight when appropriate. After ANOVA analysis, the posthoc test was performed to identify the groups that demonstrate statistically significant differences. Patients with no/first-degree injuries were compared with patients with second-degree injuries and OASI, as well as with participants who gave birth by cesarean section. Significance was set at p < 0.05. Analysis of power was performed in the initial study of the project; because the current study is a follow-up study involving the same cohort, power analysis was not applicable. All the data were compiled in an encoded database using Microsoft Access for Office 365, Version 1908 (Microsoft Corp., Redmond, WA, USA). Analyses were performed using SPSS Version 24 (IBM SPSS Statistics, Armonk, NY).
Ethical approval was granted by the Regional Ethical Review Board of Umeå (Nr. 2016-458-31 M). Informed consent was obtained from all participants. All data were deidentified prior to analysis. All experiments were performed in accordance with relevant guidelines and regulations. Patients could choose at any stage of the study to terminate their participation.