Figure 7 | Scientific Reports

Figure 7

From: Hyperbaric oxygen suppressed tumor progression through the improvement of tumor hypoxia and induction of tumor apoptosis in A549-cell-transferred lung cancer

Figure 7

The reduced p53 protein level by HBOT was modulated through a ubiquitin-dependent proteasome degradation in A549 cells. The HBOT A549 and Beas-2 cell were treated with 3.5 ATA for 90 min in the presence of 5 mM MG132 (a proteasome degradation inhibitor) or 2 mM 3-MA(an autophagy inhibitor) for 4 h. These cell lysates were subject to the Western analysis for antibodies against p53, LC3B, HIF-1α, γH2A.x, Nrf2, PARP, and control protein ACTN. The results are representative of two independent experiments. Quantitative analysis of the western blot was listed under each strip.

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