The HBOT protocol and its transient effect on A549 and Beas-2 cell lines. (A) The HBOT protocol was applied in A549 and Beas-2 cell lines. (B) The cell viability profiles were analyzed in A549 and Beas-2 cell lines. (C,D) The HBOT A549 and Beas-2 cells were treated with indicated conditions and then cell lysates were subject to the (C) Western analysis for antibodies against p53, LC3B, and control protein ACTN and (D) RT-PCR analysis for p53 mRNA and control GAPDH mRNA. The results were representative of two independent experiments. Quantitative analysis of the western blot was listed under each strip.