Figure 1 | Scientific Reports

Figure 1

From: A bispecific antibody agonist of the IL-2 heterodimeric receptor preferentially promotes in vivo expansion of CD8 and NK cells

Figure 1

Bispecific antibodies specific to IL-2Rβ and IL-2Rγ induce phosphorylation of STAT5. (A) Summary of IL-2RB and IL-2RG monospecific UniAb binders identified during antibody lead discovery. (B,C,D) Heatmaps showing fold-induction of phosphorylated STAT5 (pSTAT5) in CD8+ T-cells from human PBMCs treated with individual monospecific UniAbs or αIL2Rβ and αIL2Rγ monospecific UniAbs in a 1:1 molar ratio mixture (B), αIL-2Rβ/γ bispecific UniAbs (C), or rhIL-2 positive control (D) at 50 nM for 1 h. In the antibody graphic on the right, the green and blue arms represent IL2RG and IL2RB binding domains, respectively. pSTAT5 levels were determined by flow cytometry and reported as geometric mean fluorescent intensity (gMFI) over the gMFI of unstimulated cells.

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