Double-immunostained labeling of KIF5B and GAP43 protein expression in the rat gastrocnemius muscle. (a) Representative image of KIF5B and GAP43 co-localization analysis showing GAP-43 (green), KIF5B (red) and myonuclei (blue). (b 1–3) Higher magnification of selected area (yellow box) in image (a). (c) GAP-43-KIF5B positive Cells (% GAP-43+) in different experimental groups. White bar = 50 µm. **p < 0.001. Statistical analysis was performed using the Graph-Pad Prism statistics software (Graph-Pad Software Inc., San Diego, CA, USA free demo version 5.04).