Figure 2 | Scientific Reports

Figure 2

From: The effects of exercise training on Kinesin and GAP-43 expression in skeletal muscle fibers of STZ-induced diabetic rats

Figure 2

Kif5B and GAP-43 expression in different experimental groups. (a) Representative image of Kif5B analysis. (e) Number of Kif5B+/100 fibers. (c) Correlation between Kif5B+/100 fibers and the number of nuclei per fiber. (d 1–4) Higher magnification of selected area (yellow box) in image (a) showing Laminin (red), Kif5B (green) and myonuclei (blue). (e) Correlation between Kif5B+/100 fibers and average gastrocnemius muscle CSA. (f) Representative image of GAP-43 analysis. (g) Number of GAP-43+/100 fibers. (h) Correlation between GAP-43+/100 fibers and the number of nuclei per fiber. (i 1–4) Higher magnification of selected area (yellow box) in image (f) showing Laminin (green), GAP-43 (red) and myonuclei (blue). (j) Correlation between GAP-43+/100 fibers and average gastrocnemius muscle CSA. (k) Representative Western blot images from different experimental groups. Expression of Kif5B (l), and GAP-43 (m) further demonstrated by Western blotting normalized to GAPDH. White bar = 50 µm. **p < 0.001. Full gel-images are available in Supplementary Material. Statistical analysis was performed using the Graph-Pad Prism statistics software (Graph-Pad Software Inc., San Diego, CA, USA free demo version 5.04).

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