Muscle fiber cross-sectional area (CSA) and satellite cell content in different experimental groups. (a) Sections of gastrocnemius muscles immunolabeled for Laminin (red) and Hoechst (blue). (b) Representative image of satellite cell showing Laminin (red), Pax7 (green) and myonuclei (blue). (c) Average gastrocnemius muscle CSA and (d) myonuclei numbers. (e) Number of Pax7+/100 fibers. White bar = 50 µm. **p < 0.001. Statistical analysis was performed using the Graph-Pad Prism statistics software (Graph-Pad Software Inc., San Diego, CA, USA free demo version 5.04).